高性能紫外可見分光光度計(jì)生產(chǎn)廠家客戶至上上海儀電分析儀器供應(yīng)-b2b網(wǎng)站_b2b電子商務(wù)平臺【買產(chǎn)品】-上?？谄蚴称酚邢薰?/title><meta name="description" content="紫外-可見吸收光譜為什么有些化合物是有色的，而另一些化合物卻沒有？共軛與顏色有什么關(guān)系？我們必須對光譜中可見光部分和附近的不同波長處的光吸收進(jìn)行精確測量。商業(yè)光譜儀可對光譜中近紫外和可見部分光吸收進(jìn)行精確測量?？梢姽鈪^(qū)域的光子能量為36-7"><meta name="keywords" content="高性能紫外可見分光光度計(jì)生產(chǎn)廠家,紫外可見分光光度計(jì)"><link rel="canonical" href="http://www.cdcfah.com/"/><meta name="applicable-device" content="pc"><link rel="shortcut icon" href="/static/new_net_01/images/favicon.ico"><link rel="stylesheet" href="/static/new_net_01/css/slick.css" /><link rel="stylesheet" href="/static/new_net_01/css/addnew.css"><link rel="stylesheet" href="/static/new_net_01/css/sidenav.css"><link rel="stylesheet" href="/static/new_net_01/css/style.css" /><link rel="stylesheet" href="/static/new_net_01/css/tab.css"><link rel="stylesheet" href="/static/new_net_01/css/404.css"> <meta name="baidu-site-verification" content="OBVzrC9aaQ" /> <meta http-equiv="Cache-Control" 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class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>高性能紫外可見分光光度計(jì)生產(chǎn)廠家,紫外可見分光光度計(jì)</p><p><span>***更新：</span>2020-11-25 12:42:42</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="3bnuwtf" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海儀電分析儀器有限公司</a></p><p><span>聯(lián)系人：</span>薛馬駿</p><p><span>郵箱: </span>ydfxwx@qq.com</p><p><span>電話: </span>18001994173</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span>http://www.inesa-a.com</p><p><span>手機(jī): </span>021-34530361</p><p><span>地址: </span>徐塘路88號7幢4層、9幢</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="3yfc2rt" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="atqxegi" class="box"><p><p> 紫外-可見吸收光譜為什么有些化合物是有色的，而另一些化合物卻沒有？共軛與顏色有什么關(guān)系？我們必須對光譜中可見光部分和附近的不同波長處的光吸收進(jìn)行精確測量。商業(yè)光譜儀可對光譜中近紫外和可見部分光吸收進(jìn)行精確測量。 </p> <p> 可見光區(qū)域的光子能量為36-72kcal/mol，近紫外線區(qū)域（至200nm）的能量范圍擴(kuò)展至143kcal/mole。波長小于200nm的紫外線輻射難以處理，因此很少用作結(jié)構(gòu)分析的常規(guī)工具。 </p> <p> 當(dāng)一束光照射物質(zhì)時，上述能量會激發(fā)分子電子至更高能量的軌道。下圖顯示了有機(jī)分子中發(fā)生的各種電子激發(fā)的示意圖，其包含六個躍遷。通常，只有三個低能量躍遷是通過200至800nm光的能量實(shí)現(xiàn)的，也就是說，能夠吸收200-800nm區(qū)域光的分子應(yīng)具有π電子系統(tǒng)和具有未成對電子對的雜原子。這種吸光基團(tuán)稱為生色團(tuán)，高性能紫外可見分光光度計(jì)生產(chǎn)廠家。 </p> <p> 當(dāng)樣品分子暴露于具有與分子內(nèi)可能的電子躍遷相匹配能量的光時，高性能紫外可見分光光度計(jì)生產(chǎn)廠家，電子受光子激發(fā)從高的占據(jù)分子軌道（HOMO）躍遷到低的未占據(jù)分子軌道（LUMO），一些光能將被吸收，所產(chǎn)生的物質(zhì)稱為激發(fā)態(tài)物質(zhì)。光譜儀記錄吸收波長以及每個波長的吸收程度，所得光譜用吸光度（A）與波長的關(guān)系圖表示，高性能紫外可見分光光度計(jì)生產(chǎn)廠家。吸光度通常在0（無吸收）到2（99％吸收）的范圍內(nèi)。在水質(zhì)的常規(guī)監(jiān)測中，紫外可見分光光度法占有較大的比重，待測物的濃度和干擾物的濃度差別很大。高性能紫外可見分光光度計(jì)生產(chǎn)廠家</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="高性能紫外可見分光光度計(jì)生產(chǎn)廠家,紫外可見分光光度計(jì)" src="https://tyunfile.71360.com/WaterMark/UploadFile/ydfx123/637396593808795839/2949755.png"></div><p> 紫外可見分光光度計(jì)條件設(shè)定 </p> <p> 在定量分析中，為了提高測定的靈敏度，入射光的波長應(yīng)選擇被測物的MAX吸收波長λmax，如果λmax有干擾，可選擇另一條靈敏度稍低、但能避免干擾的譜線，所以，適當(dāng)選擇入射光的波長，不僅能提高測定的靈敏度，還能提高測定的準(zhǔn)確度。 </p> <p> 狹縫寬度過大，入射光的單色性差；狹縫寬度太小，入射光的強(qiáng)減弱。狹縫寬度過大或過小均會造成靈敏度降低，選擇是產(chǎn)生MIN誤差情下的MAX狹縫。一般選用儀器的狹縫寬琶度應(yīng)小于待測樣品吸收帶的半寬度，否則測得的吸光度值會偏低，狹縫寬度的選擇應(yīng)以減少狹縫寬度時供試品的吸光度不再；加為準(zhǔn)，對于大部分被測品種，可以使用2nm縫寬。 </p> <p> 可通過控制被測物濃度或改變吸收池厚度來實(shí)現(xiàn)吸光度合理的范圍，以盡量減小測量誤差。一般吸光度盡量控制在0.1-0.8范圍。 </p> <p> 空白溶液是用來調(diào)節(jié)吸光度測量工作零點(diǎn)即A=0，T=100%的溶液，以消除溶液中其它基體組分以及吸收池和溶劑對入射光的反射和吸收所帶來的誤差。根據(jù)情況不同，常用空白溶液有如下幾種選擇。 </p> <p> <br /> 專業(yè)紫外可見分光光度計(jì)初次使用紫外可見分光光度計(jì)的人員，一般來說，一定要詳細(xì)閱讀儀器安全操作手冊。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="高性能紫外可見分光光度計(jì)生產(chǎn)廠家,紫外可見分光光度計(jì)" src="https://tyunfile.71360.com/WaterMark/UploadFile/ydfx123/637396593771266494/7369055.png"></div><p> 色譜技術(shù)比它起步晚了二百多年，但如今的色譜儀器（氣相、液相、氣質(zhì)、液質(zhì)）占了分析界的大半壁江山。相較璀璨如星的各類的色譜儀器，紫外-可見分光光度計(jì)黯然了很多。 </p> <p> 紫外-可見分光光度計(jì)可以被更精確更高級的檢測手段所取代？可以退出“分析史”的舞臺？ </p> <p> NO！NO！NO！ </p> <p> 事實(shí)上紫外-可見分光光度計(jì)（下文簡稱紫外分光光度計(jì)），依然是“分析舞臺”的美玉，低調(diào)有內(nèi)涵，親民有價值。一起來發(fā)現(xiàn)它在日化產(chǎn)品分析中的美和價值吧。原理分子的紫外可見吸收光譜是由于分子中的某些基團(tuán)吸收了紫外可見輻射光后，發(fā)生了電子能級躍遷而產(chǎn)生的吸收光譜。由于各種物質(zhì)具有各自不同的分子、原子和分子空間結(jié)構(gòu)，其吸收光能量的情況也就不會相同，因此，每種物質(zhì)都有其特有的、固定的吸收光譜曲線，可根據(jù)吸收光譜上某些特征波長處吸光度的高低判別或測定該物質(zhì)的含量，這就是分光光度定性和定量分析的基礎(chǔ)。 </p><p> 當(dāng)以空白試劑為參比調(diào)節(jié)儀器零點(diǎn)時，比色皿窗材料的吸收、內(nèi)外窗面的散射以及溶劑的吸收等都被抵消，所得吸光度值完全由顯色溶液中待測離了濃度所決定。此時吸光度值與試液濃度一般呈線性關(guān)系。吸光度值與測定濃度不成直線關(guān)系，發(fā)生向下彎曲(負(fù)偏離)或向上彎曲(正偏離)的現(xiàn)象而偏離朗伯一比爾定律的主要原因如下。 </p> <p> <br /> </p> <p> (1)非單色光的影響：紫外一可見分光光度訃使用連續(xù)光源和分光器分光，不可能得到真正理想的單色光；為保證在測量中能得到充分的光強(qiáng)，儀器必須保持一定的狹縫寬度。由狹縫射出后投射到被測物質(zhì)上的光，是一個有限寬度的譜帶——光譜通帶。隨光譜通帶寬度的增大，吸收光譜的分辨率降低，并偏離朗伯一比爾定律。 </p> <p> (2)非吸收光的影響：當(dāng)來自出射狹縫的光的光譜通帶寬度大于吸收光譜譜帶時，投射在被測物質(zhì)上的光就含有非吸收光。這不僅能使靈敏度降低，且能使校準(zhǔn)曲線向橫坐標(biāo)軸彎曲而偏離朗伯一比爾定律。傳統(tǒng)單光束UV紫外測試，測試過程全程封閉，單光束光源只通過光柵直接照射樣品，再經(jīng)光電倍增管檢測器檢測。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="高性能紫外可見分光光度計(jì)生產(chǎn)廠家,紫外可見分光光度計(jì)" src="https://tyunfile.71360.com/WaterMark/UploadFile/ydfx123/637396593980700101/5381945.png"></div><p> 紫外可見分光光度計(jì)的比色皿的洗滌方法 </p> <p> 1分光光度法中比色皿潔凈與否是影響測定準(zhǔn)確度的因素之一。因此，必須重視選擇正確的洗凈方法。 </p> <p> 俺的經(jīng)驗(yàn)是：要是你測定溶液是酸，如果不干凈，就用弱堿溶液洗，要是你測定溶液是堿，如果不干凈，就用弱酸溶液洗，要是你測定溶液是有機(jī)物質(zhì)，如果不干凈，就用有機(jī)溶劑，比如酒精等溶液洗。 </p> <p> 2選擇比色皿洗滌液的原則是去污效果好，不損壞比色皿，同時又不影響測定。 </p> <p> 2.1分析常用的鉻酸洗液不宜用于洗滌比色皿，這是因?yàn)閹谋壬笤谠撓匆褐杏袝r會局部發(fā)熱，致使比色皿膠接面裂開而損壞。同時經(jīng)洗液洗滌后的比色皿還很可能殘存微量鉻，其在紫外區(qū)有吸收，因此會影響鉻及其他有關(guān)元素的測定。 </p> <p> 一般主張使用硝酸和過氧化氫(5：1)的混合溶液泡洗，然后用水沖洗干凈。對一般方法難以洗凈的比色皿，還可以采取以下兩種方法。 </p> <p> 2.2先將比色皿侵入含有少量陰離子表面活性劑的碳酸鈉(20克/升)溶液泡洗，經(jīng)水沖洗后，再于過氧化氫和硝酸(5：1)混合溶液中侵泡半小時。在分光光度計(jì)的使用或檢定中,透射比(吸光度)的準(zhǔn)確度直接關(guān)系到所測數(shù)據(jù)的可信性及科學(xué)性。高性能紫外可見分光光度計(jì)生產(chǎn)廠家</p><p style="text-indent:25px">實(shí)驗(yàn)室研究人員希望省錢購入專門儀器定量核酸、蛋白或者細(xì)菌的生長情況。高性能紫外可見分光光度計(jì)生產(chǎn)廠家</p><p> 紫外可見分光光度計(jì)的特點(diǎn)分光光度法對于分析人員來說，可以說是常用和有效的工具之一。幾乎每一個分析實(shí)驗(yàn)室都離不開紫外可見分光光度計(jì)。分光光度法具有以下主要特點(diǎn)。 </p> <p> 1靈敏度高 </p> <p> 由于新的顯色劑的大量合成，并在應(yīng)用研究方面取得了可喜的進(jìn)展，使得對元素測定的靈敏度有所推進(jìn)，特別是有關(guān)多元絡(luò)合物和各種表面活性劑的應(yīng)用研究，使許多元素的摩爾吸光系數(shù)由原來的幾萬提高到數(shù)十萬。 </p> <p> 2選擇性好 </p> <p> 目前已有些元素只要利用控制適當(dāng)?shù)娘@色條件就可直接進(jìn)行光度法測定，如鈷、鈾、鎳、銅、銀、鐵等元素的測定，已有比較滿意的方法了。 </p> <p> 3準(zhǔn)確度高 </p> <p> 對于一般的分光光度法，其濃度測量的相對誤差在1～3%范圍內(nèi)，如采用示差分光光度法進(jìn)行測量，則誤差可減少到0.X%。 </p> <p> 4適用濃度范圍廣 </p> <p> 可從常量(1%～50%)(尤其使用示差法)到痕量(10-8～10-6%)(經(jīng)預(yù)富集后)。 </p> <p> 5分析成本低、操作簡便、快速、應(yīng)用廣 </p> <p> 由于各種各樣的無機(jī)物和有機(jī)物在紫外可見區(qū)都有吸收，因此均可借此法加以測定。到目前為止，幾乎化學(xué)元素周期表上的所有元素(除少數(shù)放射性元素和惰性元素之外)均可采用此法。在國際上發(fā)表的有關(guān)分析的論文總數(shù)中，光度法約占28%，我國約占所發(fā)表論文總數(shù)的33%。高性能紫外可見分光光度計(jì)生產(chǎn)廠家</p><p style="text-indent:25px">上海儀電分析儀器有限公司是一家儀器儀表、電子元器件及相關(guān)的系統(tǒng)集成的生產(chǎn)、服務(wù)及銷售，計(jì)算機(jī)軟件開發(fā)及銷售，從事貨物進(jìn)出口及技術(shù)進(jìn)出口業(yè)務(wù)?！疽婪毥?jīng)批準(zhǔn)的項(xiàng)目，經(jīng)相關(guān)部門批準(zhǔn)后方可開展經(jīng)營活動】儀器儀表、電子元器件及相關(guān)的系統(tǒng)集成的生產(chǎn)、服務(wù)及銷售，計(jì)算機(jī)軟件開發(fā)及銷售，從事貨物進(jìn)出口及技術(shù)進(jìn)出口業(yè)務(wù)。【依法須經(jīng)批準(zhǔn)的項(xiàng)目，經(jīng)相關(guān)部門批準(zhǔn)后方可開展經(jīng)營活動】的公司，是一家集研發(fā)、設(shè)計(jì)、生產(chǎn)和銷售為一體的專業(yè)化公司。儀電分析深耕行業(yè)多年，始終以客戶的需求為向?qū)В瑸榭蛻籼峁?**的分光光度計(jì)，氣相色譜，原子吸收，食品安全。儀電分析不斷開拓創(chuàng)新，追求出色，以技術(shù)為先導(dǎo)，以產(chǎn)品為平臺，以應(yīng)用為重點(diǎn)，以服務(wù)為保證，不斷為客戶創(chuàng)造更高價值，提供更優(yōu)服務(wù)。儀電分析始終關(guān)注自身，在風(fēng)云變化的時代，對自身的建設(shè)毫不懈怠，高度的專注與執(zhí)著使儀電分析在行業(yè)的從容而自信。</p></p><br /><p>文章來源地址: 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