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id="f3wfbnu" class="twoCol"><div id="v2jvm8j" class="page-proshow clearfix"><p class="current"> 當(dāng)前位置:<a href="http://www.cdcfah.com/">首頁?<a href="http://www.cdcfah.com/cp/">產(chǎn)品供應(yīng)</a>?<a href="http://www.cdcfah.com/cp/first-_yyby/">醫(yī)藥健康</a>?<a href="http://www.cdcfah.com/cp/second-yyby_swzp/">生物制品</a>?<a href="http://www.cdcfah.com/cp/third-swzp_qtswzp/">其他生物制品</a>?江蘇特色熙寧生物專業(yè)大分子生物分析平臺(tái) 寧波熙寧檢測(cè)技術(shù)供應(yīng)</p><div id="3mdfwnk" class="leftbox clearfix"><h1>江蘇特色熙寧生物專業(yè)大分子生物分析平臺(tái) 寧波熙寧檢測(cè)技術(shù)供應(yīng)</h1><div id="ud7hzbi" class="proBig"><div id="besta7h" class="bigimg"><div><p class="pic"><img src="https://tyunfile.71360.com/WaterMark/UploadFile/Accurantbio/637413944123728184/5882543.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="lp3oa7c" class="smallimg"><div><p class="pic"><img src="https://tyunfile.71360.com/WaterMark/UploadFile/Accurantbio/637413944123728184/5882543.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="ngkbx3u" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>江蘇特色熙寧生物專業(yè)大分子生物分析平臺(tái),熙寧生物專業(yè)大分子生物分析平臺(tái)</p><p><span>***更新：</span>2020-12-16 07:11:47</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="7p28m4n" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">寧波熙寧檢測(cè)技術(shù)有限公司</a></p><p><span>聯(lián)系人：</span>王蒙</p><p><span>郵箱: </span>meng.wang@accurantbio.com</p><p><span>電話: </span>13585708897</p><p><span>傳真: </span>0574_</p><p><span>網(wǎng)址: </span>https://www.accurantbio.com</p><p><span>手機(jī): </span>0574-87878487</p><p><span>地址: </span>浙江省寧波市鄞州區(qū)聚賢路587弄A5幢4樓</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a 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style="text-indent:25px">3.光電管和檢測(cè)系統(tǒng)：經(jīng)熒光染色的細(xì)胞受合適的光激發(fā)后所產(chǎn)生的熒光是通過光電轉(zhuǎn)換器轉(zhuǎn)變成電信號(hào)而進(jìn)行測(cè)量的。光電倍增管（PMT）常用。4.計(jì)算機(jī)和分析系統(tǒng)：經(jīng)放大后的電信號(hào)被送往計(jì)算機(jī)分析器。流式細(xì)胞檢測(cè)的臨床應(yīng)用1.免疫學(xué)領(lǐng)域：淋巴細(xì)胞亞群檢測(cè)，用以評(píng)估機(jī)體的免疫狀態(tài)、輔助疾病如AIDS/HIV的診斷等，是目前各醫(yī)療單位開展和應(yīng)用的項(xiàng)目。2.血液學(xué)領(lǐng)域：血液系統(tǒng)疾病的免疫分型及白血病微小殘留病變(minimalresidualdisease，MRD)監(jiān)測(cè)，用于血液系統(tǒng)疾病的診斷、評(píng)估和復(fù)發(fā)監(jiān)測(cè)。FCM的細(xì)胞免疫分型是國(guó)際公認(rèn)的診斷造血細(xì)胞疾病必不可少的重要標(biāo)準(zhǔn)之一，是目前被接受和認(rèn)可的免疫分型方法。3.臨床：主要是DNA倍體和細(xì)胞周期分析，了解細(xì)胞DNA倍體和增殖能力，異常倍體的出現(xiàn)意味著DNA合成的異常，江蘇特色熙寧生物專業(yè)大分子生物分析平臺(tái)，可能是病變前發(fā)生的重要標(biāo)志。另外，F(xiàn)ACS還可以檢測(cè)分析相關(guān)標(biāo)志物（細(xì)胞的增殖活性標(biāo)志分子、分化標(biāo)志分子、凋亡標(biāo)志分子以及免疫學(xué)標(biāo)志物），用于發(fā)病機(jī)制的研究、個(gè)性化方案的制定以及預(yù)后判斷等。4.臨床細(xì)胞：流式細(xì)胞儀是在細(xì)胞領(lǐng)域的一個(gè)重要的研究和研發(fā)依托，江蘇特色熙寧生物專業(yè)大分子生物分析平臺(tái)，江蘇特色熙寧生物專業(yè)大分子生物分析平臺(tái)。熙寧生物專注于大分子生物檢測(cè)。精翰生物采用相同的Raji細(xì)胞和Blinatumomab類似物抗體，建立了Blinatumomab的細(xì)胞平臺(tái)的生物分析方法。江蘇特色熙寧生物專業(yè)大分子生物分析平臺(tái)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇特色熙寧生物專業(yè)大分子生物分析平臺(tái),熙寧生物專業(yè)大分子生物分析平臺(tái)" src="https://tyunfile.71360.com/WaterMark/UploadFile/Accurantbio/637413944123728184/5882543.png"></div><p style="text-indent:25px">色譜和質(zhì)譜技術(shù)在醫(yī)藥研究中扮演著越來越重要的角色，從早期研發(fā)、表征分析、工藝優(yōu)化到CMC、QC分析均有應(yīng)用。熙寧生物，致力于更好的生物質(zhì)譜檢測(cè)技術(shù)開發(fā)與應(yīng)用，從組學(xué)全覆蓋研究到單分子深入分析，逐步聚焦，只為實(shí)現(xiàn)更好的生物質(zhì)譜應(yīng)用；從科技服務(wù)到醫(yī)藥研究，打通機(jī)制研究到臨床應(yīng)用的全通路。熙寧生物以生物科技成就他人、造福大眾。圖1質(zhì)譜在科技服務(wù)和生物醫(yī)藥中的應(yīng)用我們所提供的的服務(wù)包括生物藥表征檢測(cè)（蛋白、多肽）、臨床前DMPK研究及生物分析。生物藥又稱為大分子藥，生物制品主要分為生物藥物和預(yù)防性生物藥物。預(yù)防性生物藥物主要指疫苗。生物藥物主要包括單克隆抗體、酶、干擾素、細(xì)胞因子和胰島素等。生物藥表征檢測(cè)服務(wù)在蛋白研究中，指的是蛋白質(zhì)的各項(xiàng)特性，包括結(jié)構(gòu)、理化、活性等一系列內(nèi)容。準(zhǔn)確的氨基酸序列、分子量、價(jià)態(tài)變化、糖基化、聚集水平以及氧化水平都是蛋白質(zhì)藥物表征的關(guān)鍵要素。具體服務(wù)項(xiàng)目如下1.純度、肽圖分析（HPLC）采用高效液相色譜法對(duì)蛋白多肽的純度和肽圖譜進(jìn)行分析，從而實(shí)現(xiàn)對(duì)蛋白質(zhì)純度和酶解后肽段分布情況的測(cè)定，對(duì)于蛋白多肽類藥物的進(jìn)一步研究具有重要意義。純度可用于工業(yè)蛋白多肽樣品雜質(zhì)與含量分析。江蘇項(xiàng)目熙寧生物專業(yè)大分子生物分析平臺(tái)ELISA的基本類型。ELISA可用于測(cè)定抗原，也可用于測(cè)定抗體，精翰生物專注于臨床大分子檢測(cè)。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇特色熙寧生物專業(yè)大分子生物分析平臺(tái),熙寧生物專業(yè)大分子生物分析平臺(tái)" src="https://tyunfile.71360.com/WaterMark/UploadFile/Accurantbio/637413944234284835/688044.png"></div><p> 簡(jiǎn)明扼要地討論了LBA測(cè)試方法在蛋白質(zhì)生物藥開發(fā)中的應(yīng)用，以及與其它生物分析平臺(tái)的比較。自上世紀(jì)60年代對(duì)胰島素進(jìn)行定量測(cè)定以來，基于與其它生物分子結(jié)合作用的LBA方法已被地用于生物分子的定量分析。例如，在蛋白質(zhì)生物藥開發(fā)過程中，這些方法為定量分析或檢測(cè)蛋白質(zhì)提供了一種準(zhǔn)確和經(jīng)濟(jì)有效的方法。測(cè)試試劑（MAbs或PAbs）生成/選擇是LBA方法開發(fā)過程中的關(guān)鍵步驟。一旦選擇好了試劑，不同測(cè)試平臺(tái)上的LBA方法能夠提供的時(shí)間和成本效益。如前所述，另一個(gè)主要的大分子生物分析平臺(tái)是液相色譜-質(zhì)譜（LC-MS/MS）。在小分子藥物的生物分析中，LC-MS/MS方法是無可置疑的；在大分子分析中，LC-MS/MS的重要性也在不斷地增加，特別是在與LBA方法以某種形式組合之后?？梢钥隙ǎ锓治鰳I(yè)界將繼續(xù)探索LC-MS/MS在蛋白質(zhì)藥物的生物分析中的應(yīng)用。生物分析行業(yè)必須證明LC-MS/MS，在藥物開發(fā)的整個(gè)過程中，與LBA方法相比較，具有重現(xiàn)性（reproducibility）、可轉(zhuǎn)移性（transferability），可靠性（reliability），和成本效益（cost–effectiveness），等方面的優(yōu)勢(shì)。</p><p> 2價(jià)），而IgM有10個(gè)抗原結(jié)合位點(diǎn)（10價(jià)）。親和度表示IgG或IgM的，分別對(duì)于2個(gè)或10個(gè)抗原分子，的整體結(jié)合強(qiáng)度（overallstrength）。圖5.競(jìng)爭(zhēng)式ELISA的詳細(xì)原理和流程圖?？乖?jìng)爭(zhēng)式ELISA（a）；抗體競(jìng)爭(zhēng)式ELISA（b）。關(guān)鍵試劑一個(gè)ELISA方法較重要的組成部分是測(cè)試試劑，它決定了ELISA方法的靈敏度、特異性和方法的質(zhì)量。ELISAs常用于藥物開發(fā)：在PK評(píng)估中，定量測(cè)定蛋白生物藥的濃度；測(cè)定內(nèi)源性蛋白和生物標(biāo)志物；檢測(cè)抗藥物抗體的存在以進(jìn)行免疫原性評(píng)估，等。LBA方法優(yōu)先的關(guān)鍵試劑是單克隆抗體（MAbs）或多克隆抗體（PAbs），而不是重組靶標(biāo)蛋白。為了產(chǎn)生PAbs或MAbs，需要將生物藥，或生物標(biāo)志物蛋白，及其佐劑或載體，根據(jù)相關(guān)應(yīng)用，給到合適的宿主動(dòng)物物種上進(jìn)行免疫。對(duì)于PAbs，兔子，山羊和綿羊是較常用的宿主物種；因?yàn)樗鼈兊拇篌w型（產(chǎn)生的抗體量也大），容易找到血管和強(qiáng)健的免疫反應(yīng)。用于免疫的每一個(gè)抗原都是高度復(fù)雜的，因此它可以呈現(xiàn)大量的，可以被不同的淋巴細(xì)胞識(shí)別的表位；這些淋巴細(xì)胞隨后被。了的淋巴細(xì)胞增殖，分化成漿細(xì)胞，并分泌出多克隆抗體的混合體。PAb庫（混合體）了不同抗體的**群體，這些抗體可以識(shí)別抗原的多個(gè)表位(用于ELISA分析)。中和抗體檢測(cè)方法中具備很好的適應(yīng)性，熙寧生物具有自主知識(shí)產(chǎn)權(quán)。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇特色熙寧生物專業(yè)大分子生物分析平臺(tái),熙寧生物專業(yè)大分子生物分析平臺(tái)" src="https://tyunfile.71360.com/WaterMark/UploadFile/Accurantbio/637413944169568322/7111777.png"></div><p> 大多數(shù)生物分析方法都基于免疫測(cè)試方法（Immunoassays），或者更廣義地稱為，配體結(jié)合式測(cè)試方法（ligandbindingassays,LBA）。這些方法涉及一系列試劑的使用，如抗藥物抗體，其它抗體，生物藥的靶標(biāo)蛋白，等。另一個(gè)主要的大分子生物分析平臺(tái)是液相色譜-質(zhì)譜（LC-MS/MS），以及LBA與LC-MS/MS在一定程度上的組合（LBA-LC-MS/MS）。總之，在大分子生物分析中，目標(biāo)分析物（analyteofinterest）是處于一個(gè)非常復(fù)雜的生物基質(zhì)背景中的蛋白質(zhì)；因此，其定量分析存在著一系列特有的挑戰(zhàn)。本系列以介紹LBA的相關(guān)基礎(chǔ)知識(shí)開始。目前，中國(guó)生物醫(yī)藥行業(yè)對(duì)于生物分析**的需求在日益增加，反映了更多的創(chuàng)新生物藥進(jìn)入臨床前動(dòng)物和臨床研究。希望此文起到分享相關(guān)知識(shí)與經(jīng)驗(yàn)，拋磚引玉的目的，引發(fā)更多的相關(guān)討論，交流和研究，并吸引有志于從事此類事業(yè)的同道加入，共同發(fā)展，加速振興中國(guó)的生物醫(yī)藥產(chǎn)業(yè)。1.生物分析中LBA測(cè)試方法的概述配體結(jié)合式測(cè)試方法（LBA，也稱為immunoassays）是一種常用的分析工具，用于，根據(jù)與其他生物分子的結(jié)合相互作用（bindinginteraction），定量測(cè)定生物分子（目標(biāo)分析物，Analyte）在生物體液中的濃度。從穩(wěn)轉(zhuǎn)細(xì)胞株構(gòu)建，方法開發(fā)優(yōu)化，到方法驗(yàn)證和樣本分析，熙寧提供一體化全流程細(xì)胞學(xué)生物分析方法服務(wù)。江蘇項(xiàng)目熙寧生物專業(yè)大分子生物分析平臺(tái)</p><p style="text-indent:25px">熙寧生物創(chuàng)始人曾參與《中國(guó)藥典2015版》“生物樣品分析方法驗(yàn)證指導(dǎo)原則”；江蘇特色熙寧生物專業(yè)大分子生物分析平臺(tái)</p><p> 用于這樣的定量分析方法。由于這些bindinginteractions的內(nèi)在性質(zhì)，LBA的標(biāo)（校）準(zhǔn)曲線的動(dòng)態(tài)（定量）范圍比較狹窄，同時(shí)也是非線性，S型的（sigmoidalcurve）。圖1.使用放射性標(biāo)記檢測(cè)試劑的液相（A）和固相（B）結(jié)合測(cè)試的原理圖。在液相測(cè)試中，結(jié)合反應(yīng)在溶液中發(fā)生；然后，結(jié)合了的與未結(jié)合的檢測(cè)試劑被分離。在固相測(cè)試中，一個(gè)關(guān)鍵試劑被動(dòng)地固定到固體表面，如微孔板或樹脂。此試劑結(jié)合目標(biāo)分析物，而目標(biāo)分析物則結(jié)合到標(biāo)記檢測(cè)試劑。2.放射免疫測(cè)定方法的誕生Yalow和Benson于1960年開發(fā)的測(cè)定人類胰島素的方法，是放射免疫測(cè)定（RIA）的標(biāo)志性例子；Yalow因此于1977年獲得諾貝爾獎(jiǎng)。簡(jiǎn)單地說，用魚精蛋白鋅牛胰島素或商業(yè)常規(guī)牛胰島素免疫豚鼠，以獲得作為該RIA方法關(guān)鍵試劑的胰島素結(jié)合抗體。在這種方法中，由于缺乏人類胰島素的結(jié)晶體，I131標(biāo)記的結(jié)晶牛胰島素（胰島素-I131）被用作示蹤劑。此RIA的原理是基于樣本中的人胰島素與豚鼠血清中的胰島素結(jié)合抗體的強(qiáng)力結(jié)合（圖2中的步驟1），并與已知濃度的胰島素-I131與該抗體的結(jié)合競(jìng)爭(zhēng)（圖2步驟2-3）。胰島素-I131與胰島素結(jié)合抗體的結(jié)合在溶液中進(jìn)行。江蘇特色熙寧生物專業(yè)大分子生物分析平臺(tái)</p><p style="text-indent:25px">寧波熙寧檢測(cè)技術(shù)有限公司坐落在外高橋自由貿(mào)易試驗(yàn)區(qū)加楓路8號(hào)503-505/浙江省寧波市鄞州區(qū)聚賢路587弄A5幢4樓，是一家專業(yè)的一般項(xiàng)目：技術(shù)服務(wù)、技術(shù)開發(fā)、技術(shù)咨詢、技術(shù)交流、技術(shù)轉(zhuǎn)讓、技術(shù)推廣；醫(yī)學(xué)研究和試驗(yàn)發(fā)展；實(shí)驗(yàn)分析儀器銷售；儀器儀表銷售（除依法須經(jīng)批準(zhǔn)的項(xiàng)目外，憑營(yíng)業(yè)執(zhí)照依法自主開展經(jīng)營(yíng)活動(dòng)）。許可項(xiàng)目：貨物進(jìn)出口，技術(shù)進(jìn)出口，進(jìn)出口代理（依法須經(jīng)批準(zhǔn)的項(xiàng)目，經(jīng)相關(guān)部門批準(zhǔn)后方可開展經(jīng)營(yíng)活動(dòng)，具體經(jīng)營(yíng)項(xiàng)目以審批結(jié)果為準(zhǔn)）。公司。公司目前擁有專業(yè)的技術(shù)員工，為員工提供廣闊的發(fā)展平臺(tái)與成長(zhǎng)空間，為客戶提供高質(zhì)的產(chǎn)品服務(wù)，深受員工與客戶好評(píng)。公司業(yè)務(wù)范圍主要包括：生物分析，臨床檢測(cè)，生物藥檢測(cè)，免疫原性檢測(cè)等。公司奉行顧客至上、質(zhì)量為本的經(jīng)營(yíng)宗旨，深受客戶好評(píng)。公司力求給客戶提供全數(shù)良好服務(wù)，我們相信誠實(shí)正直、開拓進(jìn)取地為公司發(fā)展做正確的事情，將為公司和個(gè)人帶來共同的利益和進(jìn)步。經(jīng)過幾年的發(fā)展，已成為生物分析，臨床檢測(cè)，生物藥檢測(cè)，免疫原性檢測(cè)行業(yè)出名企業(yè)。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/2426976.html</p></div></div></div></div></div><div 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