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src="https://tyunfile.71360.com/UploadFile/saisitaike/637382890836210937/2290151.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="iu7299m" class="smallimg"><div><p class="pic"><img src="https://tyunfile.71360.com/UploadFile/saisitaike/637382890836210937/2290151.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="m9zjfho" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>阜新培養(yǎng)基制備器直銷,培養(yǎng)基制備器</p><p><span>***更新：</span>2020-12-27 05:16:53</p><p><span id="click" >瀏覽次數(shù)：8次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="3o9u8se" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">賽鍶鈦氪(上海)貿(mào)易有限公司</a></p><p><span>聯(lián)系人：</span>郭經(jīng)理</p><p><span>郵箱: </span>1071226099@qq.com</p><p><span>電話: </span>15601635108</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span>http://www.systec-lab.com.cn</p><p><span>手機(jī): </span>021-60190256</p><p><span>地址: </span>沈磚公路6000弄1號(hào)206室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="z43azln" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="uakm2uw" class="box"><p><p style="text-indent:25px">培養(yǎng)基的分裝：培養(yǎng)基的分裝，應(yīng)按使用的目的和要求，分裝于試管、燒瓶等適當(dāng)容器內(nèi)。分裝量不得超容器裝盛量的2/3。容器口可用墊有防濕紙的棉塞封堵，其外還須用防水紙包扎（現(xiàn)試管一般多有用螺旋蓋者）。分裝時(shí)好好能使用半自動(dòng)或電動(dòng)的定量分裝器。分裝瓊脂斜面培養(yǎng)基時(shí)，分裝量應(yīng)以能形成2/3底層和1/3斜面的量為洽當(dāng)。分裝容器應(yīng)預(yù)先清洗干凈并經(jīng)干烤消毒，以利于培養(yǎng)基的徹底滅菌。每批培養(yǎng)基應(yīng)另外分裝20ml培養(yǎng)基于一小玻璃瓶中，阜新培養(yǎng)基制備器直銷，隨該批培養(yǎng)基同時(shí)滅菌，阜新培養(yǎng)基制備器直銷，以為測(cè)定該批培養(yǎng)基好終pH之用，阜新培養(yǎng)基制備器直銷。培養(yǎng)基制備器應(yīng)存放于冷暗處，較好能放于普通冰箱內(nèi)。阜新培養(yǎng)基制備器直銷</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="阜新培養(yǎng)基制備器直銷,培養(yǎng)基制備器" src="https://tyunfile.71360.com/UploadFile/saisitaike/637382890836210937/2290151.png"></div><p style="text-indent:25px">培養(yǎng)基制備方法與注意事項(xiàng):1、培養(yǎng)基配方選擇同一種培養(yǎng)基的配方在不同著作中常會(huì)有某些差別。因此，除所用的是標(biāo)準(zhǔn)方法，應(yīng)嚴(yán)格按其規(guī)定進(jìn)行配制外，一般均應(yīng)盡量收集有關(guān)資料，加以比較核對(duì)，再依據(jù)自己的使用目的，加以選用，記錄其來(lái)源。 2、培養(yǎng)基制備記錄每次制備培養(yǎng)基均應(yīng)有記錄，包括培養(yǎng)基名稱，配方及其來(lái)源，和各種成份的牌號(hào)，好終pH值、消毒的溫度和時(shí)間制備的日期和制備者等，記錄應(yīng)復(fù)制一份，原記錄保存?zhèn)洳?，?fù)制記錄隨制好的培養(yǎng)基一同存放、以防發(fā)生混亂。 3、培養(yǎng)基成份稱量培養(yǎng)基的各種成分必須精確稱取并要注意防止錯(cuò)亂，好好一次完成，不要中斷。可將配方置于傍側(cè)，每稱完一種成分即在配方上做出記號(hào)，并將所需稱取的藥品一次取齊，置于左側(cè)，每種稱取完畢后，即移放于右側(cè)。完全稱取完畢后，還應(yīng)進(jìn)行一次檢查。阜新培養(yǎng)基制備器直銷培養(yǎng)基制備器解凍血清時(shí)，請(qǐng)按照的逐步解凍法(-20℃至4℃至室溫)。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="阜新培養(yǎng)基制備器直銷,培養(yǎng)基制備器" src="https://tyunfile.71360.com/UploadFile/saisitaike/637382890967626953/2525624.png"></div><p style="text-indent:25px">血清解凍后發(fā)現(xiàn)有絮狀沉淀物出現(xiàn)，該如何處理：血清中沉淀物的出現(xiàn)有許多種原因，但好普遍的原因是由于血清中脂蛋白的變性所造成的，而血纖維蛋白（形成凝血的蛋白之一）在血渭解凍后，也會(huì)存在于血清中，亦是造成沉淀物的主要原因之一。但這些絮狀沉淀物并不影響血清本身的質(zhì)量。若想去除這些絮狀沉淀物，可以將血清分裝至無(wú)菌離心管內(nèi)，以400g稍微離心，上清液即可接著加入培養(yǎng)基內(nèi)一起過(guò)濾。不建議以過(guò)濾的方法去除這些絮狀沉淀物，因?yàn)樗赡軙?huì)阻塞過(guò)濾膜。何謂熱滅活：一般是以56℃, 30分鐘來(lái)處理已解凍的血清，因?yàn)榇思訜岵襟E可以使補(bǔ)體去活化，而補(bǔ)體所參與的反應(yīng)有：溶細(xì)胞活性，平滑肌的收縮，肥大細(xì)胞和血小板組胺的釋放，增強(qiáng)吞噬作用，淋巴細(xì)胞、巨噬細(xì)胞的趨化和啟動(dòng)。</p><p style="text-indent:25px">微生物限度: 細(xì)胞培養(yǎng)基不是無(wú)菌產(chǎn)品，其中的微生物在一定條件下會(huì)吸收細(xì)胞培養(yǎng)基中的營(yíng)養(yǎng)物質(zhì)滋生繁殖，導(dǎo)致細(xì)胞培養(yǎng)基變質(zhì)失效?？刂萍?xì)胞培養(yǎng)基中細(xì)菌和霉菌，是延長(zhǎng)細(xì)胞培養(yǎng)基有效期的方法之一。清大天一在參考《中華人民當(dāng)?shù)厮幍洹?005版二部附錄第100頁(yè)的口服給藥制劑的微生物限度標(biāo)準(zhǔn)，并嚴(yán)于該標(biāo)準(zhǔn)，規(guī)定細(xì)胞培養(yǎng)基產(chǎn)品的細(xì)菌數(shù)每1g不得超過(guò)200個(gè)，霉菌數(shù)每1g不得超過(guò)50個(gè)。稱取樣品1g，加入無(wú)菌純化水10mL溶解，混勻即得試樣。按中華人民當(dāng)?shù)厮幍?005年版二部附錄ⅪJ微生物限度檢查法進(jìn)行，檢查項(xiàng)目為細(xì)菌數(shù)、霉菌數(shù)。檢查法采用平皿法。培養(yǎng)基制備器解凍血清時(shí)，請(qǐng)隨時(shí)將之搖晃均勻，使溫度及成分均一，減少沉淀的發(fā)生。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="阜新培養(yǎng)基制備器直銷,培養(yǎng)基制備器" src="https://tyunfile.71360.com/UploadFile/saisitaike/637382891282480469/5765409.png"></div><p style="text-indent:25px">培養(yǎng)基的滅菌：一般培養(yǎng)基可采用121℃ 高壓蒸汽滅菌15分鐘的方法。在各種培養(yǎng)基制備方法中，如無(wú)特殊規(guī)定，即可用此法滅菌。某些畏熱成分，如糖類，應(yīng)另行配成20% 如或更高的濃液，以過(guò)濾或間歇滅菌法消毒，以后再用無(wú)菌操作技術(shù)、定量加于培養(yǎng)基。明膠培養(yǎng)基亦應(yīng)用較低溫度滅菌。血液、體液和物品等則應(yīng)從無(wú)菌操作技術(shù)抽取和加入于經(jīng)冷卻約50℃ 左右的培養(yǎng)基中。瓊脂斜面培養(yǎng)基應(yīng)在滅菌后立即取出、待冷至55-60℃時(shí)，擺置成適當(dāng)斜面、待其自然凝固。培養(yǎng)基制備器分裝好的培養(yǎng)基應(yīng)立即進(jìn)行滅菌。蘇州培養(yǎng)基制備器哪家便宜</p><p style="text-indent:25px">培養(yǎng)基制備器功能強(qiáng)大的加熱器，能夠迅速加熱。阜新培養(yǎng)基制備器直銷</p><p style="text-indent:25px">過(guò)濾：配成的培養(yǎng)基，若無(wú)特殊要求，可省略此步。若有沉淀或渾濁，需要澄清的，液體培養(yǎng)基可用濾紙過(guò)濾。而固體培養(yǎng)基可用中間夾一薄層脫脂棉的雙層紗布過(guò)濾。如果過(guò)濾法還不能達(dá)到澄清的要求，則可用蛋清澄清法，即培養(yǎng)基加熱冷卻到50℃~60℃，裝入三角瓶?jī)?nèi)（不超過(guò)容量的二分之一），按1000ml加入1個(gè)~2個(gè)雞蛋的蛋清，用力搖晃3min~5min，高壓蒸汽滅菌121℃、20min，取出趁熱過(guò)濾即可。擺斜面：裝在試管中的瓊脂培養(yǎng)基，在滅菌完成后，趁熱立即擺放在木棒（或玻璃棒）上，并完成適時(shí)地斜度，冷卻后，瓊脂凝固即成斜面。斜面長(zhǎng)度不要超過(guò)試管的二分之一。阜新培養(yǎng)基制備器直銷</p></p><br /><p>文章來(lái)源地址: 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