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style="color: #f30;"></span></p><p><span>產品關鍵詞：</span>江蘇四色免疫組化技術服務,免疫組化</p><p><span>***更新：</span>2020-08-22 10:08:19</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="mwoqlsp" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網址: </span></p><p><span>手機: </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="ap2axub" class="page-proshow02"><p class="title">詳細說明</p><div id="pjbd2qx" class="box"><p><p> 收藏查看我的收藏0有用+1已投票0免疫組化編輯鎖定討論免疫組化，是應用免疫學基本原理——抗原抗體反應，即抗原與抗體特異性結合的原理，通過化學反應使標記抗體的顯色劑（熒光素、酶、金屬離子、同位素）顯色來確定組織細胞內抗原（多肽和蛋白質），對其進行定位、定性及相對定量的研究，稱為免疫組織化學技術(immunohistochemistry)或免疫細胞化學技術(immunocytochemistry)。中文名免疫組化外文名immunocytochemistry應用免疫學基本原理稱為免疫組織化學技術目錄1基本原理2分類3作用?標本?抗體?常用染色方法4操作步驟?操作步驟?化染色步驟5判定分析6意義?免疫組化鏡檢?細胞凋亡檢測?關于掉片7經驗總結?實驗為例?免疫個人感悟免疫組化基本原理編輯抗體和抗原之間的結合具有高度的特異性，免疫組織化學正是利用了這一原理。先將組織或細胞中的某種化學物質提取出來，以此作為抗原或半抗原，通過免疫動物后獲得特異性的抗體，江蘇四色免疫組化技術服務，再以此抗體去探測組織或細胞中的同類的抗原物質，江蘇四色免疫組化技術服務，江蘇四色免疫組化技術服務。由于抗原與抗體的復合物是無色的，因此還必須借助于組織化學的方法將抗原抗體結合的部位顯示出來，以其達到對組織或細胞中的未知抗原進行定性，定位或定量的研究。江蘇四色免疫組化技術服務</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇四色免疫組化技術服務,免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750107422/8042334.png"></div><p> 中級——問題求助和不斷總結；高級——難題解答和經驗分享這三個階段，也學到了不少知識，積累了很多寶貴經驗，與大家一起分享下。免疫組化實驗為例從我接觸的很多本科生和研究生中發(fā)現(xiàn)，他們的確是免疫組化“新手”，他們只注重如何做和**終的結果，但對為什么這樣做不太感興趣。他們常按照網上所說的方法，摸索好的抗體濃度和條件后做出很漂亮的染色結果后，他們就認為自己已經掌握了免疫組化方法了，結果不求上進，更換一種新抗體后或實驗出現(xiàn)異常結果后就很難做出來了。當然，免疫組化對于我來說，做過很多，但也不能說什么都會，只不過我做的多了，失敗多了和思考多了，可能比新手多了解一些其中的訣竅，拿來與大家進行分享。此外，我個人經驗不可能面面俱到，還需要更多有經驗的高手一起分享、糾正和補充。在這里，我需要感謝中華病理網、丁香通、小木蟲論壇給我很大的幫助，還有上海舜田生物技術人員老師給我實驗很多指導和幫助，讓我受益匪淺。免疫組化免疫個人感悟1、其實免疫組化，我個人感覺方法和操作都不是很難，關鍵是結果出現(xiàn)異常時該如何去解決？我認為首先需要掌握好免疫組化實驗的原理，知道每一個操作步驟的目的。江蘇四色免疫組化技術服務</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇四色免疫組化技術服務,免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899749443360/7546740.png"></div><p> 我一般初次做一種新抗體，喜歡先用說明書建議的低濃度和高濃度做一次，然后決定是向高還是低方向摸索。一般先決定二抗的濃度（工作液就好辦了，直接滴加），重點摸索一抗的**適濃度。注意：免疫反應中存在前帶和后帶效應，這提示不是濃度越高，陽性率就越高，反之亦然。⑷重要原因排除之后，也不要忽視抗體的質量：原裝抗體一般比較穩(wěn)定，效果較好；而進口分裝次之；工作液可能要注意質量問題。3、同時，DAB的孵育時間可能要適當延長，在鏡下觀察，有時可延長至30min。但一般3-10min比較好，此時背景也較淺。否則，說明抗體濃度不合適。4、***，血清封閉時間也可相應縮短。一般10-30min，但這個時間可以調整，封閉主要是降低切片的總體背景著色。5、此外，細胞通透也不可忽視。許多戰(zhàn)友認為石蠟切片一般不需細胞通透，因為切片時可能已經把細胞切開了，但對于胞**白，建議還是通透一下，促進抗體等試劑充分進入參與反應。6、以上原因，都是針對實際中常見原因來進行分析的，前提是排除操作者的操作錯誤而引起陰性結果，這就需要新手還是要設置陽性對照以排除方法的問題。還有抗體稀釋液的pH值過低等其它原因的干擾?？傊?，要想把免疫組化做好，可能每一個環(huán)節(jié)都很重要。</p><p> 具體時間需要摸索，而濃度一般有工作液，若是濃縮液還要摸索濃度，切記要避光反應。但在免疫熒光中我們一般先把二抗?jié)舛群头跤龝r間先定下，然后去摸索一抗?jié)舛群头跤龝r間。***，熒光素標記的二抗隨著保存時間的延長，可能后有大量的游離熒光素殘留，需要注意配制時小包裝和并進行適當?shù)碾x心。6）復染目的是形成細胞輪廓，從而更好對目標蛋白進行定位。一般常用DAPI復染。7）封片為了長期保存，我們一般用緩沖甘油等封片，此外還有專門的抗熒光萃滅封片液。避免產生氣泡，方法是直接在載玻片組織上滴一滴封片液，然后一手拿住蓋片某一拐角，而另一手拿對面的那個拐角，接近封片液近端的拐角先降低，直至接觸到液體時為止；當發(fā)現(xiàn)液體接觸面在不斷彌散時，則可以緩慢降低另一拐角，這樣一般不會產生氣泡。8）切片清洗為了防止一抗、二抗等試劑殘留而引起非特異性染色，所以適當?shù)丶訌娗逑矗ㄑ娱L時間和增多次數(shù)）尤為重要，我一般在一抗孵育前的清洗是3min*3次，而一抗孵育后的清洗均為5次*5min。注意：⑴單獨沖洗，防止交叉反應造成污染。⑵溫柔沖洗，防止切片的脫落。我喜歡用浸洗方式；⑶沖洗的時間要足夠，才能徹底洗去結合的物質。⑷PBS的PH和離子強度的使用和要求。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇四色免疫組化技術服務,免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750458985/5221034.png"></div><p style="text-indent:25px">【多色免疫組化染色樣本要求】1福爾馬林固定的蠟塊或玻片，大塊組織或TMA都可以，封蠟不能有明顯的破損。2玻片樣本，組織需要緊貼玻片，避免褶皺，玻片不能有破損、劃傷或污漬。3組織**小應包含大于1000個細胞。4蠟塊需包埋實體瘤組織，壞死瘤組織、細針穿刺物、細胞甩片樣品會影響染色效果。5組織應當用10%中性福爾馬林固定，正常固定時間為18～24小時。6切片厚度為4μm左右，使用防脫玻片。建議玻片在固定后一周內制備為佳。7不要在水浴撈片環(huán)節(jié)添加任何粘合劑，樣本應置于玻片正面、**。將撈片豎直置于吸水紙上去除水分，輕敲去除水滴，不要用紙擦拭玻片。8玻片置于45°C熱盤上放置30min（自然風干玻片時長不小于1小時）。【設備耗材】1檢驗所需設備器材：熒光顯微鏡、通風櫥、移液器、恒溫干燥箱、微波爐、計時器2檢測所需耗材器皿：免疫組化筆、微波修復杯、染色缸、孵育濕盒、蓋玻片、洗瓶、量筒100ml、量筒1000ml等【試劑制備】1通用試劑：DMSO、miejun去離子水、二甲苯、乙醇（全部、95%、70%）、10%中性福爾馬林、抗原修復液（推薦使用佰諾全景酸性或堿性抗原修復液）、一抗封閉液等。天津多標記免疫組化染色服務</p><p style="text-indent:25px">江蘇四色免疫組化技術服務</p><p> 在肌肉、皮下、靜脈或腹腔內注射；·抗體效價的檢測：加強免疫一周后，耳緣靜脈**–抗體效價測定可用環(huán)狀沉淀試驗、瓊脂雙向擴散法等方法。前者抗體效價在1:4000以上，后者在1:16以上，即可**，取血清進一步提純。舉例2：微量抗原淋巴結內注射免疫家兔·一般選擇～–先在其兩腳墊注射完全福氏佐劑(卡介苗每兔合5mg)；–10天后，見胭窩淋巴結腫大，然后將抗原注射至腫大的淋巴結內，***次注射抗原用完全福氏佐劑混合乳化；–以后每隔20天用不完全福氏佐劑乳化抗原，以同樣方法加強2次，各次注射的抗原均為25～50?g；–末次注射兩周后兔耳放血測價(可達1:128)。舉例3：豚鼠和大鼠的免疫·初次免疫–用10～100?gAg加入FCA，在背部皮內注射4～6點，每點，也可肌肉內或皮下注射；·加強免疫–每隔7～8天，將10～50?gAg于PBS或FIA中。在肌肉、皮下或靜脈注射；·抗體效價的檢測(4)免疫劑量·抗原性強的抗原量應小，過大反會引起免疫***；·免疫周期長的動物可少量多次注射，短的可大量少次。(4)免疫劑量(續(xù))·各種動物***免疫抗原劑量和加強免疫的劑量(5)抗體效價的檢測多采免疫雙向擴散法【基本原理】·指抗原和抗體在同一凝膠內都擴散，彼此相遇后形成特異性的沉淀線。江蘇四色免疫組化技術服務</p><p style="text-indent:25px">上海朝瑞生物科技有限公司是一家1.發(fā)布科研技術 2.發(fā)布應用技術 3.發(fā)布專業(yè)專長 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗檢測服務 7.發(fā)布協(xié)同代研發(fā)服務 8.發(fā)布升級改造產品服務 9.發(fā)布實驗室及儀器設備共享 10.發(fā)布培訓基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項目團隊招聘13.發(fā)布研究生婚戀 14.發(fā)布項目整包服務 15.發(fā)布資源交換業(yè)務的公司，是一家擁有雇員人數(shù)優(yōu)異員工的企業(yè)。公司自成立以來，以質量為發(fā)展，讓匠心彌散在每個細節(jié)，公司旗下[ "科研技術服務", "應用技術服務", "科研成果轉化", "科學產品銷售" ]深受客戶的喜愛。公司將不斷增強企業(yè)重點競爭力，努力學習行業(yè)先進知識，遵守行業(yè)規(guī)范，植根于化工行業(yè)的發(fā)展。公司憑借創(chuàng)新的產品、專業(yè)的服務、眾多的成功案例積累起來的聲譽和口碑，讓企業(yè)發(fā)展再上新高。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/1000289.html</p></div></div></div></div></div><div id="hbtvmjv" class="page-right twoColRt"><div id="pzr2h7e" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="n8lp77a" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" alt="福建ZF配件哪家強信息推薦自業(yè)物資供應" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></div><p>福建ZF配件哪家強信息推薦自業(yè)物資供應</p></a></div><div id="otvspb7" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/846569.html"><div><img src="https://tyunfile.71360.com/WaterMark/UploadFile/nbzxsw/637298235378037109/764794.png" alt="寧波寧海直接考A2有幾個科目真誠推薦寧波助學商務信息咨詢供應" class="vcenter" 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