上海微滴式數(shù)字PCR定量檢測(cè)服務(wù) 來電咨詢上海朝瑞生物科技供應(yīng)-b2b網(wǎng)站_b2b電子商務(wù)平臺(tái)【買產(chǎn)品】-上?？谄蚴称酚邢薰?/title><meta name="description" content="數(shù)字PCR對(duì)環(huán)境目標(biāo)基因的監(jiān)測(cè)，需要對(duì)復(fù)雜樣品中低濃度的目標(biāo)進(jìn)行準(zhǔn)確檢測(cè)。ddPCR每次運(yùn)行中能創(chuàng)建數(shù)千個(gè)PCR反應(yīng)室，帶來了目標(biāo)的準(zhǔn)確測(cè)定，即使它們濃度很低，因此可以實(shí)現(xiàn)對(duì)土壤、污水、淤泥、酒泥等為樣本的環(huán)境生物學(xué)研究和病原微生物監(jiān)測(cè)。數(shù)"><meta name="keywords" content="上海微滴式數(shù)字PCR定量檢測(cè)服務(wù),數(shù)字PCR"><link rel="canonical" href="http://www.cdcfah.com/"/><meta name="applicable-device" content="pc"><link rel="shortcut icon" href="/static/new_net_01/images/favicon.ico"><link rel="stylesheet" href="/static/new_net_01/css/slick.css" /><link rel="stylesheet" href="/static/new_net_01/css/addnew.css"><link rel="stylesheet" href="/static/new_net_01/css/sidenav.css"><link rel="stylesheet" href="/static/new_net_01/css/style.css" /><link rel="stylesheet" href="/static/new_net_01/css/tab.css"><link rel="stylesheet" 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class="container clearfix tcPro"><section class="home-main container clearfix"><div id="7dr2sjf" class="twoCol"><div id="irdkbn3" class="page-proshow clearfix"><p class="current"> 當(dāng)前位置:<a href="http://www.cdcfah.com/">首頁?<a href="http://www.cdcfah.com/cp/">產(chǎn)品供應(yīng)</a>?<a href="http://www.cdcfah.com/cp/second-_nongye/">農(nóng)業(yè)</a>?<a href="http://www.cdcfah.com/cp/third-nongye_nlmyxmhz/">農(nóng)林牧漁項(xiàng)目合作</a>?上海微滴式數(shù)字PCR定量檢測(cè)服務(wù) 來電咨詢上海朝瑞生物科技供應(yīng)</p><div id="ck7j8py" class="leftbox clearfix"><h1>上海微滴式數(shù)字PCR定量檢測(cè)服務(wù) 來電咨詢上海朝瑞生物科技供應(yīng)</h1><div id="3yl28mt" class="proBig"><div id="8qhtqhv" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909800390625/81991.jpg" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="8nt3jah" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909800390625/81991.jpg" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="zbiuqct" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>上海微滴式數(shù)字PCR定量檢測(cè)服務(wù),數(shù)字PCR</p><p><span>***更新：</span>2020-08-24 13:11:59</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="dfh3jwt" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="edpgsub" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="2r88kb2" class="box"><p><p> 數(shù)字PCR對(duì)環(huán)境目標(biāo)基因的監(jiān)測(cè)，需要對(duì)復(fù)雜樣品中低濃度的目標(biāo)進(jìn)行準(zhǔn)確檢測(cè)。ddPCR每次運(yùn)行中能創(chuàng)建數(shù)千個(gè)PCR反應(yīng)室，帶來了目標(biāo)的準(zhǔn)確測(cè)定，即使它們濃度很低，因此可以實(shí)現(xiàn)對(duì)土壤、污水、淤泥、酒泥等為樣本的環(huán)境生物學(xué)研究和病原微生物監(jiān)測(cè)。數(shù)字PCR也可以用于檢測(cè)一個(gè)生物中是否存在某特定DNA序列，上海微滴式數(shù)字PCR定量檢測(cè)服務(wù)，這被稱為基因型鑒定。例如，基因型鑒定可通過發(fā)現(xiàn)樣品中是否存在某種群特異性序列來判斷樣本的真實(shí)性?；蛐丸b定還可用于法醫(yī)分析判斷在現(xiàn)場(chǎng)發(fā)現(xiàn)的DNA樣品是否和嫌疑人的吻合，上海微滴式數(shù)字PCR定量檢測(cè)服務(wù)，可用于刑偵基因?qū)W領(lǐng)域。掌握數(shù)學(xué)，上海微滴式數(shù)字PCR定量檢測(cè)服務(wù)、物理、化學(xué)等方面的基本理論和基本知識(shí)。上海微滴式數(shù)字PCR定量檢測(cè)服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海微滴式數(shù)字PCR定量檢測(cè)服務(wù),數(shù)字PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909800390625/81991.jpg"></div><p style="text-indent:25px">PCR擴(kuò)增過程在芯片上實(shí)現(xiàn)。**終對(duì)陽性微滴計(jì)數(shù)從而得到精細(xì)的核酸***數(shù)量。作為***三個(gè)熒光通道檢測(cè)的數(shù)字PCR平臺(tái)，Naica? crystal數(shù)字PCR系統(tǒng)可在2小時(shí)內(nèi)實(shí)現(xiàn)多達(dá)36個(gè)目標(biāo)基因的檢測(cè)。Naica? Crystal系統(tǒng)由Geode微滴生成；擴(kuò)增儀和Prism3微滴閱讀分析系統(tǒng)組成，*需***耗材——Sapphire芯片，即可完成從加樣、微滴生成和擴(kuò)增，直到采集數(shù)據(jù)獲得結(jié)果。 r> Naica? crystal數(shù)字PCR可用于：基因表達(dá)差異檢測(cè)；拷貝數(shù)變異(CNV)；低豐度及稀有序列的精確定量；甲基化含量鑒定；*****的伴隨診斷；無創(chuàng)產(chǎn)前篩查；病原微生物的檢測(cè)(***、細(xì)菌等)；二代測(cè)序輔助建庫；*****的實(shí)時(shí)監(jiān)控 (ctDNA檢測(cè))等。合肥高靈敏數(shù)字PCR定量檢測(cè)服務(wù)將大量稀釋后的核酸溶液分散至芯片的微反應(yīng)器或微滴中。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海微滴式數(shù)字PCR定量檢測(cè)服務(wù),數(shù)字PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909586328125/5976755.jpg"></div><p> 采用***靶向技術(shù)，一次性針對(duì)樣本中2500余種代謝物進(jìn)行穩(wěn)定檢測(cè)，***覆蓋氨基酸、有機(jī)酸、核酸、碳水化合物、甾醇脂、脂肪酰、鞘脂、甘油脂、甘油磷脂、輔酶及維生素等多個(gè)類別，能夠挖掘更豐富、更精細(xì)、更有效的代謝組學(xué)數(shù)據(jù)。 </p> <p> 樣本要求：1.血清、血漿：200ul/sample;液氮速凍-80℃保存，干冰寄送;應(yīng)盡量避免溶血。2.組織：200mg/sample;液氮速凍后-80℃保存，干冰寄送。 3.糞便及腸道內(nèi)容物：500mg/sample;液氮速凍后-80℃保存，干冰寄送。 4.細(xì)胞：≥1×107/sample;細(xì)胞樣本請(qǐng)分裝成兩份，分別用于兩種提取方法。 </p><p style="text-indent:25px">當(dāng)前核酸分子的定量有三種方法，光度法基于核酸分子的吸光度來定量；實(shí)時(shí)熒光定量PCR（Real Time PCR）基于Ct值，Ct值就是指可以檢測(cè)到熒光值對(duì)應(yīng)的循環(huán)數(shù)；數(shù)字PCR是***的定量技術(shù)，基于單分子PCR方法來進(jìn)行計(jì)數(shù)的核酸定量，是一種***定量的方法。主要采用當(dāng)前分析化學(xué)熱門研究領(lǐng)域的微流控或微滴化方法，將大量稀釋后的核酸溶液分散至芯片的微反應(yīng)器或微滴中，每個(gè)反應(yīng)器的核酸模板數(shù)少于或者等于1個(gè)。這樣經(jīng)過PCR循環(huán)之后，有一個(gè)核酸分子模板的反應(yīng)器就會(huì)給出熒光信號(hào)，沒有模板的反應(yīng)器就沒有熒光信號(hào)。根據(jù)相對(duì)比例和反應(yīng)器的體積，就可以推算出原始溶液的核酸濃度。DNA聚合酶依賴的酶促合成反應(yīng)，擴(kuò)增的特異性取決于引物與模板DNA的特異結(jié)合。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海微滴式數(shù)字PCR定量檢測(cè)服務(wù),數(shù)字PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909658583985/2801442.jpg"></div>數(shù)字PCR是一種核酸分子精確定量技術(shù)。是一個(gè)在模板DNA、引物(模板片段兩端的已知序列)和四種脫氧核苷酸等存在的情況下，DNA聚合酶依賴的酶促合成反應(yīng)，擴(kuò)增的特異性取決于引物與模板DNA的特異結(jié)合。相較于qPCR，數(shù)字PCR可讓你能夠直接數(shù)出DNA分子的個(gè)數(shù)，是對(duì)起始樣品的精確定量。 <p> 數(shù)字 PCR 的工作原理在于將 DNA 或 cDNA 樣品分割為許多單獨(dú)、平行的 PCR 反應(yīng)，部分這些反應(yīng)包含了靶標(biāo)分子（陽性），而其他不包含（陰性）。 </p> <p> 單個(gè)分子可以被擴(kuò)增一百萬倍或更多。 </p> <p> 在擴(kuò)增期間，TaqMan® 化學(xué)試劑及染料標(biāo)記探針可用于檢測(cè)特定序列的靶標(biāo)。當(dāng)不存在任何靶標(biāo)序列時(shí)，沒有信號(hào)累積。 PCR 分析后，陰性反應(yīng)片段用于生成樣品中靶標(biāo)分子的精確計(jì)數(shù)，而無需標(biāo)準(zhǔn)品或內(nèi)標(biāo)。以改良物種質(zhì)量和生命大分子特性或生產(chǎn)特殊用途的生命大分子物質(zhì)等。上海微滴式數(shù)字PCR定量檢測(cè)服務(wù)</p><p style="text-indent:25px">Ct值就是指可以檢測(cè)到熒光值對(duì)應(yīng)的循環(huán)數(shù)；數(shù)字PCR是***的定量技術(shù)，基于單分子PCR方法來進(jìn)行計(jì)數(shù)的核酸。上海微滴式數(shù)字PCR定量檢測(cè)服務(wù)</p><p> 數(shù)字PCR實(shí)際上是一個(gè)在模板DNA、引物(模板片段兩端的已知序列)和四種脫氧核苷酸等存在的情況下，DNA聚合酶依賴的酶促合成反應(yīng)，擴(kuò)增的特異性取決于引物與模板DNA的特異結(jié)合。bio-radQX200微滴式數(shù)字PCR(ddPCR)系統(tǒng)可對(duì)DNA或RNA分子進(jìn)行定量分析，QX200Droplet數(shù)字PCR(DigitalPCR)系統(tǒng)的應(yīng)用領(lǐng)域：**生物標(biāo)志物研究和拷貝數(shù)變異分析以高靈敏度和準(zhǔn)確度測(cè)量**突變的變異程度、檢測(cè)稀有的DNA靶拷貝以及解析拷貝數(shù)變異狀態(tài)。病原體檢測(cè)精確地對(duì)靶DNA或RNA分子中的細(xì)微變化進(jìn)行定量分析，從而檢測(cè)和監(jiān)測(cè)病原體。與新一代測(cè)序無縫對(duì)接無需使用標(biāo)準(zhǔn)曲線，直接對(duì)NGS庫執(zhí)行定量分析。上海微滴式數(shù)字PCR定量檢測(cè)服務(wù)</p><p style="text-indent:25px">上海朝瑞生物科技有限公司始建于2003-01-22，坐落于上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室，現(xiàn)有員工5~10人余人。在上海朝瑞科技近多年發(fā)展歷史，公司旗下現(xiàn)有品牌zrbiorise,SPRICELL,scienceladde等。公司堅(jiān)持以客戶為中心、1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗(yàn)檢測(cè)服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級(jí)改造產(chǎn)品服務(wù) 9.發(fā)布實(shí)驗(yàn)室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項(xiàng)目團(tuán)隊(duì)招聘13.發(fā)布研究生婚戀 14.發(fā)布項(xiàng)目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù)市場(chǎng)為導(dǎo)向，重信譽(yù)，保質(zhì)量，想客戶之所想，急用戶之所急，全力以赴滿足客戶的一切需要。上海朝瑞科技始終以質(zhì)量為發(fā)展，把顧客的滿意作為公司發(fā)展的動(dòng)力，致力于為顧客帶來***的[ "科研技術(shù)服務(wù)", "應(yīng)用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學(xué)產(chǎn)品銷售" ]。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/1023687.html</p></div></div></div></div></div><div id="f8kkwno" class="page-right twoColRt"><div id="smtlsuq" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="pdelc8u" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" alt="福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></div><p>福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)</p></a></div><div id="hrdpwi7" class="recomPic likePro"><a 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