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class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="zdac2n8" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909759482422/522171.jpg" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="sqcegic" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>溫州專業(yè)數(shù)字PCR檢測服務(wù),數(shù)字PCR</p><p><span>***更新：</span>2020-08-28 04:12:32</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="7utfho9" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="8uwyvxr" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="3surtkp" class="box"><p><p style="text-indent:25px">applied biosystems于2013年也推出了產(chǎn)品，Quant Studio 3D數(shù)字PCR系統(tǒng)，溫州專業(yè)數(shù)字PCR檢測服務(wù)，這也是目前數(shù)字PCR市場上***型號(hào)的產(chǎn)品，溫州專業(yè)數(shù)字PCR檢測服務(wù)。采用高密度的納升流控芯片技術(shù)，樣本均勻分配至20,000個(gè)單獨(dú)的反應(yīng)孔中。在整個(gè)工作流程中，樣本之間保持完全隔離，可以有效地防止樣品交叉污染，溫州專業(yè)數(shù)字PCR檢測服務(wù)，減少移液過程，簡化操作步驟。同時(shí)芯片式設(shè)計(jì)避免了微滴式系統(tǒng)可能面臨的管路堵塞問題。作為applied biosystems在OpenArray芯片平臺(tái)之外推出的全新的芯片式數(shù)字PCR系統(tǒng)，值得一提的是，這個(gè)全新的系統(tǒng)在設(shè)計(jì)理念上綜合考慮了系統(tǒng)穩(wěn)定性與運(yùn)行成本因素，直接反映了該系統(tǒng)“適合所有分子生物學(xué)實(shí)驗(yàn)室使用的數(shù)字PCR系統(tǒng)”的市場定位。2013年，Thermo Fisher收購applied biosystems。Ct值就是指可以檢測到熒光值對應(yīng)的循環(huán)數(shù)；數(shù)字PCR是***的定量技術(shù)，基于單分子PCR方法來進(jìn)行計(jì)數(shù)的核酸。溫州專業(yè)數(shù)字PCR檢測服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="溫州專業(yè)數(shù)字PCR檢測服務(wù),數(shù)字PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909759482422/522171.jpg"></div><p style="text-indent:25px">DNase I footprinting是一種精確測定DNA結(jié)合蛋白在DNA分子上的結(jié)合位點(diǎn)的方法。凝膠阻滯實(shí)驗(yàn)（EMSA）和DNase I足跡分析實(shí)驗(yàn)是目前兩種常用體外研究核酸與蛋白相互作用的方法，凝膠阻滯實(shí)驗(yàn)可以確定轉(zhuǎn)錄因子是否與DNA直接結(jié)合，而DNase I足跡分析實(shí)驗(yàn)則可以精確鑒定其結(jié)合的DNA序列，從而幫助我們研究基因轉(zhuǎn)錄調(diào)控的機(jī)制。本技術(shù)服務(wù)利用熒光標(biāo)記法結(jié)合測序的方法來進(jìn)行DNase I足跡分析實(shí)驗(yàn)。檢測靈敏高，獲得的保護(hù)區(qū)域清晰，圖片的質(zhì)量好。溫州專業(yè)數(shù)字PCR檢測服務(wù)由于生物技術(shù)將會(huì)為解決人類面臨的重大問題如糧食、健康、環(huán)境、能源等開辟廣闊的前景。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="溫州專業(yè)數(shù)字PCR檢測服務(wù),數(shù)字PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909630185547/4431438.jpg"></div><p style="text-indent:25px">數(shù)字PCR(d PCR)是近年來引起重視并迅速發(fā)展起來的一種突破性的核酸定量分析技術(shù)。該技術(shù)先將核酸模板進(jìn)行稀釋,分配到大量**的反應(yīng)單元中,使每個(gè)反應(yīng)單元中只有單個(gè)模板分子,然后進(jìn)行PCR擴(kuò)增反應(yīng),擴(kuò)增結(jié)束后對每個(gè)反應(yīng)室的熒光信號(hào)進(jìn)行統(tǒng)計(jì)學(xué)分析,來定量DNA拷貝數(shù)。數(shù)字PCR采用先擴(kuò)增后定量,因此不依賴擴(kuò)增曲線的循環(huán)閾值(Ct),也無需采用內(nèi)參基因和標(biāo)準(zhǔn)曲線,準(zhǔn)確度高、重現(xiàn)性好,可以實(shí)現(xiàn)精確定量分析。由于其獨(dú)特的技術(shù)優(yōu)勢,已經(jīng)在臨床診斷、轉(zhuǎn)基因成分定量、單細(xì)胞基因表達(dá)分析、環(huán)境微生物檢測和下一代測序等研究領(lǐng)域顯示出巨大的優(yōu)勢和應(yīng)用前景。</p><p> 數(shù)字PCR技術(shù)目前在糖尿病中的研究熱點(diǎn)，通過定量檢測非甲基化DNA的水平，對I型糖尿病β細(xì)胞死亡進(jìn)行定量，從而監(jiān)控病情發(fā)展。無創(chuàng)傷的產(chǎn)前診斷, 如21號(hào)染色體為3倍體的唐氏綜合征、已知父母基因型的胎兒地中海貧血或胎兒遺傳性耳聾基因檢測等。目前無創(chuàng)檢測標(biāo)本來源主要為孕婦血液，而待檢測的胎兒DNA受到母體DNA的干擾，影響了檢測的準(zhǔn)確性。數(shù)字PCR技術(shù)可以作為二代測序法的補(bǔ)充來進(jìn)行無創(chuàng)傷產(chǎn)前診斷，從而提高工作效率、降低檢測成本。因此特別適用于依靠Ct值不能很好分辨的應(yīng)用領(lǐng)域：拷貝數(shù)變異、突變檢測、基因相對表達(dá)研究。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="溫州專業(yè)數(shù)字PCR檢測服務(wù),數(shù)字PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909691865235/2119471.jpg"></div><p style="text-indent:25px">QX200 Droplet數(shù)字PCR(Digital PCR) 系統(tǒng)的應(yīng)用領(lǐng)域： **生物標(biāo)志物研究和拷貝數(shù)變異分析以***的靈敏度和準(zhǔn)確度測量**突變的變異程度、檢測稀有的 DNA 靶拷貝以及解析拷貝數(shù)變異狀態(tài)。病原體檢測精確地對靶 DNA 或 RNA 分子中的細(xì)微變化進(jìn)行定量分析，從而檢測和監(jiān)測病原體。與新一代測序無縫對接無需使用標(biāo)準(zhǔn)曲線，直接對 NGS 庫執(zhí)行***定量分析。基因表達(dá)分析可對少量 mRNA 和 miRNA 的細(xì)微變化進(jìn)行準(zhǔn)確及重復(fù)性較好的檢測。環(huán)境監(jiān)測使用 QX200 系統(tǒng)可測試多種環(huán)境樣品，例如土壤和水。食品檢測采用經(jīng)過驗(yàn)證的 ddPCR 方法對轉(zhuǎn)基因生物體 (GMO) 進(jìn)行評(píng)估。這樣的好處是每個(gè)樣品形成20,000個(gè)液滴，而其他系統(tǒng)只能分成760-3,000個(gè)部分。分得越多，意味分析越準(zhǔn)確。溫州專業(yè)數(shù)字PCR檢測服務(wù)</p><p style="text-indent:25px">如今，你無需糾結(jié)了，因?yàn)閿?shù)字PCR（digital PCR）來了。溫州專業(yè)數(shù)字PCR檢測服務(wù)</p><p> PCR實(shí)際上是一個(gè)在模板DNA、引物(模板片段兩端的已知序列)和四種脫氧核苷酸等存在的情況下，DNA聚合酶依賴的酶促合成反應(yīng)，擴(kuò)增的特異性取決于引物與模板DNA的特異結(jié)合。 </p> <p> 數(shù)字PCR(Digital PCR)儀器伯樂生命bio-rad QX200 微滴式數(shù)字PCR (ddPCR) 系統(tǒng)可對DNA或RNA分子進(jìn)行***定量分析，適用于 EvaGreen 或基于探針的數(shù)字PCR應(yīng)用領(lǐng)域。溫州專業(yè)數(shù)字PCR檢測服務(wù)</p> 數(shù)字 PCR 的工作原理在于將 DNA 或 cDNA 樣品分割為許多單獨(dú)、平行的 PCR 反應(yīng)，部分這些反應(yīng)包含了靶標(biāo)分子（陽性），而其他不包含（陰性）。<p style="text-indent:25px">上海朝瑞生物科技有限公司創(chuàng)立于2003-01-22，總部位于上海市，是一家1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗(yàn)檢測服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級(jí)改造產(chǎn)品服務(wù) 9.發(fā)布實(shí)驗(yàn)室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項(xiàng)目團(tuán)隊(duì)招聘13.發(fā)布研究生婚戀 14.發(fā)布項(xiàng)目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù)的公司。上海朝瑞科技擁有一支經(jīng)驗(yàn)豐富、技術(shù)創(chuàng)新的專業(yè)研發(fā)團(tuán)隊(duì)，以高度的專注和執(zhí)著為客戶提供科研技術(shù)服務(wù)，應(yīng)用技術(shù)服務(wù)，科研成果轉(zhuǎn)化，科學(xué)產(chǎn)品銷售。上海朝瑞科技不斷開拓創(chuàng)新，追求***，以技術(shù)為先導(dǎo)，以產(chǎn)品為平臺(tái)，以應(yīng)用為**，以服務(wù)為保障，不斷為客戶創(chuàng)造更高價(jià)值，提供更優(yōu)服務(wù)。上海朝瑞科技創(chuàng)始人仲緒軍，始終關(guān)注客戶，以優(yōu)化創(chuàng)新的科技，竭誠為客戶提供極好的服務(wù)。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/1066411.html</p></div></div></div></div></div><div id="otq3ebv" class="page-right twoColRt"><div id="77fe8fm" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="dct739f" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" alt="福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)" class="vcenter" 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