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href="http://www.cdcfah.com/cp/third-nongye_nlmyxmhz/">農(nóng)林牧漁項目合作</a>?江蘇四步完成RNA提取試劑盒信賴推薦 **** 上海朝瑞生物科技供應(yīng)</p><div id="ge2xrie" class="leftbox clearfix"><h1>江蘇四步完成RNA提取試劑盒信賴推薦 **** 上海朝瑞生物科技供應(yīng)</h1><div id="ksjarsz" class="proBig"><div id="7wpbxof" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739418066406/2610716.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="veljkbh" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739418066406/2610716.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="2pghiug" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>江蘇四步完成RNA提取試劑盒信賴推薦,RNA提取試劑盒</p><p><span>***更新：</span>2020-08-30 16:07:21</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="cvm7vvf" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機: </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="esexiou" class="page-proshow02"><p class="title">詳細說明</p><div id="8ag8vqh" class="box"><p><p> Trizol法提取RNA實驗步驟及原理 </p> <p> 1.在提取組織RNA時,每50～100mg組織用1mL Trizol試劑對組織進行裂解；提取細胞RNA時,先用PBS洗滌/離心沉淀細胞，江蘇四步完成RNA提取試劑盒信賴推薦,每5-10 ╳106個細胞加1mL Trizol后, 反復(fù)用吹打或劇烈振蕩以裂解細胞. </p> <p> <br /> </p> <p> 2、將組織或細胞的Trizol裂解液轉(zhuǎn)入EP管中，江蘇四步完成RNA提取試劑盒信賴推薦, 在室溫15~30℃下放置5分鐘；在EP管中,按照每1mL TRIZOL加0.2mL氯仿的量加入氯仿(即為1/5體積), 蓋上EP管蓋子, 在手中用力震蕩15秒(10次), 在室溫下（15℃～30℃）放置2～3分鐘后, 14000rpm（4℃）離心15分鐘. </p> <p> 【注：離心后分為3層，RNA在上層水相, DNA在中間層, 蛋白質(zhì)在下層有機相】 </p> <p> <br /> </p> <p> 3，江蘇四步完成RNA提取試劑盒信賴推薦、取上層水相置于新EP管中(～500uL, 用200uL的黃色*頭小心吸取, 約吸取3次, 杜絕取到中間層/下層), 按照每1mL Trizol加0.5mL異丙醇的量加入異丙醇(即為1/2體積), 4℃下靜置20分鐘, 沉淀RNA；14000rpm（4℃）離心15分鐘. </p> <p> </p> <p> 4、按照每1mL TRIZOL加1mL75%乙醇進行洗滌(即為1倍體積, 注:用DEPC水配置75%乙醇), 混合(#用移液器輕輕吹打即可,不用將沉淀打碎), 14000rpm（4℃）離心20分鐘, 棄上清(#比較好用真空泵吸去上清)；讓沉淀的RNA在室溫下自然干燥(#或在超凈臺中小風(fēng)吹干,比較好置于干凈的環(huán)境中，防止灰塵雜質(zhì)進入)；用45uL Rnase-free water 溶解RNA沉淀. 但是均存在價格高、訂貨周期長的問題（如果碰上國內(nèi)無貨的時候，要從國外發(fā)貨。江蘇四步完成RNA提取試劑盒信賴推薦</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇四步完成RNA提取試劑盒信賴推薦,RNA提取試劑盒" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739418066406/2610716.png"></div><p> 上海朝瑞生物科技有限公司是一家成立于2003年的****，總部坐落于上海。公司自成立以來，秉承“真誠服務(wù)、合作雙贏”的經(jīng)營理念和良好的運作模式，現(xiàn)已發(fā)展成為產(chǎn)品種類豐富、技術(shù)力量雄厚、配套設(shè)施齊全的前列生物技術(shù)企業(yè)。我公司擁有良好的進貨渠道及國外采購合作伙伴，能夠為您提供全球數(shù)百家公司的數(shù)百萬種產(chǎn)品的現(xiàn)貨及期貨訂購服務(wù) </p> <p> 產(chǎn)品主要應(yīng)用于分子生物學(xué)、免疫學(xué)、細胞生物學(xué)、**、神經(jīng)生物學(xué)、干細胞、***等相關(guān)領(lǐng)域的生命科學(xué)研究以及***研發(fā)。同時，致力于生物技術(shù)***如單克隆抗體、重組蛋白***、***菌株、快速診斷等研究開發(fā)與技術(shù)服務(wù)。上海9分鐘完成RNA提取試劑盒50T/690元離心收集細胞。每106動物、植物和酵母細胞或每107細菌細胞加1ml裂解液混勻。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇四步完成RNA提取試劑盒信賴推薦,RNA提取試劑盒" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739423857422/1376989.png"></div><p style="text-indent:25px">. 樣品處理a. 植物組織：取新鮮或-70℃凍存100mg組織在液氮中研磨，把粉末加入到1ml裂解液中混勻。b. 動物組織：取新鮮或-70℃凍存100mg組織加1ml裂解液，用組織研磨杵或勻漿器勻漿處理。c. 貼壁細胞：直接在培養(yǎng)板中加入裂解液裂解細胞，每106細胞加1ml 裂解液。用取樣器吹打混勻。d. 細胞懸液：離心收集細胞。每106動物、植物和酵母細胞或每107細菌細胞加1ml裂解液混勻。e. 血液處理：取0.2-1ml新鮮血液加3倍體積紅細胞裂解液，混勻后室溫放置10分鐘， 10000rpm離心1分鐘。棄上清，若沉淀含有紅細胞，可加入2倍體積紅細胞裂解液重復(fù)裂解步驟。離心后沉淀加入1 ml裂解液混勻。</p><p> <br /> </p> <p> </p> <p> <br /> </p> <p> RNA 常用提取方法（1） </p> <p> 1、異硫氰酸胍氯化銫超速離心法: </p> <p> 原理：使用蛋白質(zhì)變性劑異硫氰酸胍有效制抑RN A 酶的活性，經(jīng)過起始密度為1.78g/m l 的氯化銫介質(zhì)，進行密度梯度超速離心，RNA沉淀于管底，而DNA與蛋白質(zhì)在上清中。使用這種方法，不但能得到高質(zhì)量的RNA ，而且能同時分離出細胞染色體DNA。 </p> <p> </p> <p> 2、鹽酸胍-有機溶劑法： </p> <p> 原理：本法有MacDonald1987年在Strohman報道的方法基礎(chǔ)上改進而成的，適用于沒有超速離心及設(shè)備的情況下提取細胞總RNA。它利用鹽酸胍制抑RNA酶，勻漿裂解細胞，有機溶劑抽提去除蛋白質(zhì),通過選擇性沉淀RNA分子而去除DNA。 </p> <p> </p> <p> 3、氯化鋰-尿素法： </p> <p> 原理：本法首先由Auffray報道,利用高濃度尿素變性蛋白質(zhì)同時制抑RNA酶，3 mol/L LiCl選擇性沉淀RNA。 </p> <p> </p> <p> <br /> </p> <p> <br /> 當(dāng)前提取效果好的是RNA提取試劑盒，但其售價較高，單次實驗費用花費太大。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇四步完成RNA提取試劑盒信賴推薦,RNA提取試劑盒" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739425371094/6565299.png"></div><p> 懸浮細胞：離心收集細胞，吸盡液體，每5×106-1×107 個細胞加入1ml Trizol，再用移液器反復(fù)吹打?！咀ⅰ考尤?Trizol 前應(yīng)避免洗滌細胞，會增加 mRNA 降解的可能性。 </p> <p> c. 動、植物組織：取-80°C 凍存的組織在液氮中充分研磨后加入適量的Trizol混勻；或者取新鮮動物或植物組織盡量剪碎，然后加入適量的 Trizol 進行勻漿處理。 </p> <p> 將勻漿樣品劇烈震蕩后在室溫條件下放置 5 分鐘以使**白體完全裂解?！咀ⅰ看瞬讲荒苁÷?，時間可以長于 5 分鐘。 </p> <p> （可選步驟）4°C，12000rpm 離心 10 分鐘，取上清?！咀ⅰ咳绻麡悠分泻休^多蛋白、脂肪、多糖等可離心除去。離心得到的沉淀中包括細胞外膜、多糖、高分子量的 DNA 等物質(zhì)，上清中含有 RNA。處理脂肪組織樣品時，上層是大量油脂，應(yīng)除去，然后再取澄清的勻漿溶液進行下一步的操作。高度依賴進口，成本居高不下，這也導(dǎo)致我國大量基因檢測企業(yè)技術(shù)門檻低、盈利困難。江蘇四步完成RNA提取試劑盒信賴推薦</p><p style="text-indent:25px">應(yīng)用場景快速延伸，**靶向診斷、病原體檢測、個體化用藥、**早篩等多個熱點方向得到了重點關(guān)注。江蘇四步完成RNA提取試劑盒信賴推薦</p><p> 向勻漿樣品中加0.2ml氯仿，蓋好管蓋，劇烈振蕩15秒，室溫放置3-5分鐘。 </p> <p> 2-8℃ 12000 rpm離心10分鐘。RNA主要在上層無色的水相中，把水相轉(zhuǎn)移到新管中，不要吸到沉淀。 </p> <p> 吸附柱前處理：在吸附柱中加入500ul 洗柱液，室溫放置2分鐘，2-8℃ 12,000 rpm離心2min，棄廢液。 </p> <p> 第4步收集的上清中加入200ul無水乙醇混勻，加入吸附柱靜置2分鐘，2-8℃ 12000rpm離心2min，棄廢液。 </p> <p> 向吸附柱中加入600ul漂洗液(使用前請先檢查是否已加入無水乙醇)，2-8℃ 12,000 rpm離心2min，棄廢液。 </p> <p> 向吸附柱中加入600ul漂洗液，2-8℃ 12,000 rpm離心2min，棄廢液。江蘇四步完成RNA提取試劑盒信賴推薦</p><p style="text-indent:25px">上海朝瑞生物科技有限公司創(chuàng)立于2003-01-22，總部位于上海市，是一家1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗檢測服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級改造產(chǎn)品服務(wù) 9.發(fā)布實驗室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項目團隊招聘13.發(fā)布研究生婚戀 14.發(fā)布項目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù)的公司。公司自2003-01-22成立以來，投身于科研技術(shù)服務(wù)，應(yīng)用技術(shù)服務(wù)，科研成果轉(zhuǎn)化，科學(xué)產(chǎn)品銷售，是化工的主力軍。上海朝瑞科技始終以本分踏實的精神和必勝的信念，影響并帶動同事和團隊取得成功。上海朝瑞科技創(chuàng)始人仲緒軍，始終關(guān)注客戶，以優(yōu)化創(chuàng)新的科技，竭誠為客戶提供極好的服務(wù)。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/1096132.html</p></div></div></div></div></div><div id="slxlc3c" class="page-right twoColRt"><div id="untklmn" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="nbxi2so" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img 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