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src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750380860/9179133.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="pos8d77" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750380860/9179133.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="t8dfhj7" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>天津多色熒光免疫組化分析服務(wù),免疫組化</p><p><span>***更新：</span>2020-08-31 01:14:26</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="qub8hok" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="yrnm3wb" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="3gpl9qm" class="box"><p><p> 而高離子強(qiáng)度則有利于分解）11)DAB顯色背景的深淺和特異性染色的深淺均可以由DAB孵育條件決定。DAB顯色時間不是固定的，主要由顯微鏡下控制顯色時間，到出現(xiàn)特異性染色較強(qiáng)而本底著色較淺時即可沖洗；DAB顯色時間很短（如幾秒或幾十秒）就出現(xiàn)很深的棕褐色，這很可能說明你的抗體濃度過高或抗體孵育時間過長，天津多色熒光免疫組化分析服務(wù)，需要下調(diào)抗體濃度或縮短你的抗體孵育時間；此外，若很短時間就出現(xiàn)背景很深，還有可能你前面的封閉非特異性蛋白不全，需要延長封閉時間；DAB顯色時間很長（如超過十幾分鐘）才出現(xiàn)陽性染色，一方面可能說明你的抗體濃度過低或孵育時間過短（比較好一抗4℃過夜）；另一方面就是封閉時間過長。12）復(fù)染目的是形成細(xì)胞輪廓，從而更好地對目標(biāo)蛋白進(jìn)行定位，經(jīng)常用蘇木素復(fù)染（胞核染料）。注意蘇木素復(fù)染時間要看當(dāng)時的室溫、溶液的新舊、目標(biāo)抗原的定位等情況，一般數(shù)秒-數(shù)分鐘，胞漿蛋白可以適當(dāng)時間長一點(diǎn)，而胞**白則要短。不過這個如果染色不理想可以補(bǔ)救的，天津多色熒光免疫組化分析服務(wù)。方法是：染色深則分化時間稍長些即可；染色淺則再置于蘇木素中染色即可。鹽酸酒精是分化，天津多色熒光免疫組化分析服務(wù)，氨水是返蘭。作用不同。片子復(fù)染完后流水振洗，然后置于鹽酸酒精中數(shù)秒（一定動作要快）后拿出流水振洗。天津多色熒光免疫組化分析服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津多色熒光免疫組化分析服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750380860/9179133.png"></div><p> 中級——問題求助和不斷總結(jié)；高級——難題解答和經(jīng)驗(yàn)分享這三個階段，也學(xué)到了不少知識，積累了很多寶貴經(jīng)驗(yàn)，與大家一起分享下。免疫組化實(shí)驗(yàn)為例從我接觸的很多本科生和研究生中發(fā)現(xiàn)，他們的確是免疫組化“新手”，他們只注重如何做和**終的結(jié)果，但對為什么這樣做不太感興趣。他們常按照網(wǎng)上所說的方法，摸索好的抗體濃度和條件后做出很漂亮的染色結(jié)果后，他們就認(rèn)為自己已經(jīng)掌握了免疫組化方法了，結(jié)果不求上進(jìn)，更換一種新抗體后或?qū)嶒?yàn)出現(xiàn)異常結(jié)果后就很難做出來了。當(dāng)然，免疫組化對于我來說，做過很多，但也不能說什么都會，只不過我做的多了，失敗多了和思考多了，可能比新手多了解一些其中的訣竅，拿來與大家進(jìn)行分享。此外，我個人經(jīng)驗(yàn)不可能面面俱到，還需要更多有經(jīng)驗(yàn)的高手一起分享、糾正和補(bǔ)充。在這里，我需要感謝中華病理網(wǎng)、丁香通、小木蟲論壇給我很大的幫助，還有上海舜田生物技術(shù)人員老師給我實(shí)驗(yàn)很多指導(dǎo)和幫助，讓我受益匪淺。免疫組化免疫個人感悟1、其實(shí)免疫組化，我個人感覺方法和操作都不是很難，關(guān)鍵是結(jié)果出現(xiàn)異常時該如何去解決？我認(rèn)為首先需要掌握好免疫組化實(shí)驗(yàn)的原理，知道每一個操作步驟的目的。上海多靶點(diǎn)免疫組化技術(shù)服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津多色熒光免疫組化分析服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899748603516/652413.png"></div><p> 但也存在主次之分，出現(xiàn)問題了，需要通過先排除主要的，再依次排除次要的--這是衡量你對免疫組化原理掌握與否的關(guān)鍵所在。案例三石蠟切片免疫熒光染色呈陰性結(jié)果或非特異性結(jié)果背景：因?qū)嶒?yàn)需要，于2008年07月04日初次做肝臟組織ZO-1（一種胞膜蛋白，緊密連接蛋白）免疫熒光染色，以前我對酶免疫組化非常熟悉，在丁香園子內(nèi)也有不少置頂貼和精華帖，但免疫熒光一直還沒做過（原理知道，但細(xì)節(jié)不太了解）。我先用石蠟切片做了5次，結(jié)果一直不理想（表現(xiàn)為未見特異性強(qiáng)染色）；近幾日，在舜田生物老師的幫助下，我又切了冰凍切片，做了2次，終于基本成功了。在這個過程中，我對免疫熒光染色技術(shù)有了全新的認(rèn)識，而前些天發(fā)出免疫熒光求助貼，回復(fù)的很少，所以有必要加強(qiáng)對免疫熒光方面的討論），不妥之處敬請指正。有人會問酶免疫組化做的好好的，為何要做免疫熒光？其實(shí)，這也是我以前思考的難題，現(xiàn)在我認(rèn)為可能胞膜蛋白含量不高，用普通免疫免疫組化可能做不出來，需要敏感的免疫熒光方法來進(jìn)行蛋白檢測（我是看許多外文文獻(xiàn)都是這樣做的，因此選擇免疫熒光染色。</p><p> 在堿性條件下，易與蛋白質(zhì)的賴氨酸?-氨基反應(yīng)而標(biāo)記在蛋白分子上。(4)碘化丙啶(PI)·是常用的DNA熒光標(biāo)記探針，可作為FITC的胞核對比染色?！I可嵌入到雙鏈DNA和RNA堿基對中并與之結(jié)合，但對堿基無特異性選擇?！け容^大吸收光譜是493nm，比較大發(fā)射光譜是630nm，呈紅色熒光。2、熒光抗體的保存·一要防止抗體失活，二要保持熒光素不脫落和不受激發(fā)猝滅。–一般認(rèn)為0～4?C可保存1～2年，-20?C可保存3～4年。–要小量分裝，防止反復(fù)凍融。–保存前需加防腐劑(濃度為1:5000～10000的硫柳汞或1:1000～5000疊氮化鈉)。3、免疫熒光的染色方法·免疫熒光染色法常用的有–直接法–間接法?原理：將熒光素標(biāo)記在相應(yīng)的抗體上，直接與相應(yīng)抗原反應(yīng)(用來檢測未知抗原)。?直接免疫熒光法的操作步驟·標(biāo)本的處理：–細(xì)胞涂片、細(xì)胞爬片浸入冷**或4%的多聚甲醛固定10min，然后用PBST(含pH)漂洗5min×3/次；–石蠟切片經(jīng)脫蠟、梯度酒精脫水后，進(jìn)行抗原修復(fù)，然后用PBST漂洗5min×3/次；·2%BSA或10%BSA37?C濕盒內(nèi)封閉30min·抗體染色：–在標(biāo)本片上滴加適當(dāng)稀釋的熒光標(biāo)記抗體(1:8或1:16稀釋)，放在濕盒中，37?C孵育30min；?直接免疫熒光法的操作步驟(續(xù))·PBS。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津多色熒光免疫組化分析服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899754550782/2606693.png"></div><p> 多光譜成像技術(shù)多光譜成像技術(shù)，可俗稱免疫熒光多標(biāo)，即將我們常見的免疫熒光雙標(biāo)提高到六標(biāo)，實(shí)現(xiàn)了在同一張組織切片上同時標(biāo)記3~6種蛋白，解決了科研中經(jīng)常遇到的標(biāo)記蛋白種類太少，無法在同一張切片上觀察不同細(xì)胞或者多種蛋白分布情況的難題。那么多光譜成像技術(shù)是什么工作原理，同時又具有哪些更加強(qiáng)大的功能？ </p> <p> 多光譜成像技術(shù)由多標(biāo)染色、圖像采集和圖像分析三大模塊組成。多標(biāo)染色采用的是Opal/TSA多標(biāo)記免疫熒光技術(shù)，打破了常規(guī)免疫熒光技術(shù)的局限性，不受一抗的種屬來源限制，能夠在同一組織切片樣本上復(fù)染多達(dá)7種熒光標(biāo)記（含DAPI在內(nèi)）。圖像采集采用了Vectra全光譜圖像采集技術(shù)，這是***一代的組織切片成像分析系統(tǒng)，創(chuàng)新性的將光譜成像和切片全景掃描功能完美融合，實(shí)現(xiàn)了**性的技術(shù)跨越，通過光譜拆分能夠去除自發(fā)熒光的干擾，有效識別多重?zé)晒庑盘?，從而獲得***圖像，確保能夠進(jìn)行精細(xì)的定量定位分析。 </p> <p> <br /> 江蘇五色免疫組化分析服務(wù)</p><p style="text-indent:25px">天津多色熒光免疫組化分析服務(wù)</p><p> 是一種小分子維生素，分子量為244，是轉(zhuǎn)氨甲?；^程中的輔酶?！た股锼?avidin)，又稱卵白素或親和素，是一種分子量為67000的堿性蛋白，對生物素具有很強(qiáng)的親和力，比抗原抗體間的親和力要高出100萬倍。它由4個亞基組成，每個亞基都有生物素的結(jié)合位點(diǎn)?！烧呔膳c抗體等大分子生物活性物質(zhì)相偶聯(lián)，又可被酶類等多種示蹤物所標(biāo)記，形成生物素-抗生物素系統(tǒng)。–該系統(tǒng)一端偶聯(lián)大分子生物反應(yīng)體系，另一端連結(jié)標(biāo)記物，后者加入酶的底物，產(chǎn)生顏色反應(yīng)。(1)標(biāo)記抗生物素—生物素法(labelledavidin-biotinmethod,LAB)：分為直接法和間接法?！ぶ苯臃ㄓ蒙锼貥?biāo)記***抗體，與抗原結(jié)合；酶標(biāo)記抗生物素，與生物素結(jié)合，然后進(jìn)行酶呈色反應(yīng)。·間接法用生物素標(biāo)記二抗，酶標(biāo)記抗生物素，先用***抗體與組織抗原結(jié)合，再將第二抗體與***抗體相連結(jié)，***進(jìn)行呈色反應(yīng)。(2)橋抗生物素一生物素法(bridgeavidin-biotinmethod，BRAB)–此法是用生物素分別標(biāo)記抗體和酶，以抗生物素為橋，把二者連接起來，進(jìn)行呈色反應(yīng)。(3)抗生物素-生物素-過氧化物酶法(ABC法)·ABC法是在BRAB和LAB的基礎(chǔ)上改良的方法?！BC復(fù)合物是將過氧化物酶結(jié)合在生物素上。天津多色熒光免疫組化分析服務(wù)</p><p style="text-indent:25px">上海朝瑞生物科技有限公司致力于化工，以科技創(chuàng)新實(shí)現(xiàn)***管理的追求。上海朝瑞科技作為1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗(yàn)檢測服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級改造產(chǎn)品服務(wù) 9.發(fā)布實(shí)驗(yàn)室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項(xiàng)目團(tuán)隊招聘13.發(fā)布研究生婚戀 14.發(fā)布項(xiàng)目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù)的品牌企業(yè)，為客戶提供質(zhì)量的科研技術(shù)服務(wù)，應(yīng)用技術(shù)服務(wù)，科研成果轉(zhuǎn)化，科學(xué)產(chǎn)品銷售。上海朝瑞科技致力于把技術(shù)上的創(chuàng)新展現(xiàn)成對用戶產(chǎn)品上的貼心，為用戶帶來***體驗(yàn)。上海朝瑞科技始終關(guān)注化工行業(yè)。海納百川，有容乃大，國內(nèi)外同行的智慧都是促使我們前行的力量。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/1100337.html</p></div></div></div></div></div><div id="ibiunj3" class="page-right twoColRt"><div id="r2hedub" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="sr7awio" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" alt="福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)" class="vcenter" 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