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id="wp2jqxu" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>天津IMAB362特異人源Claudin18.2蛋白,人源Claudin18.2蛋白</p><p><span>***更新：</span>2020-09-01 06:16:01</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="fzqx7sk" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="lpmyipw" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="3ipbiug" class="box"><p><p> 3%H202:30uL。h.終止液H2S04:lmol/L。操作方法包被先取96孔ELISA板，將(ug/ml)，按100uL/孔加入板孔中，4。C包被過(guò)夜；倒掉包被液，加入封閉液，室溫下封存2h;棄去封閉液，用洗滌液洗6次，每次2min;分別加入倍比稀釋的山羊抗人(500ug/ml)和抗血清，100wL/孔，室溫下孵育lh;棄去抗體和抗血清，用洗滌液洗6次，每次3min;加入HRP標(biāo)記的抗山羊二抗，100nL/孔，天津IMAB362特異人源Claudin18.2蛋白，室溫孕育lh;棄去二抗，用洗滌液洗6次，每次3min;加入底物顯色液，100uL/孔，室溫，顯色10-20min;加入終止液，天津IMAB362特異人源Claudin18.2蛋白，50"L/孔，終止反應(yīng)；酶標(biāo)儀讀數(shù)，測(cè)定沒(méi)孔在4卯nm的吸光值。免疫印跡反應(yīng)測(cè)定血清抗，結(jié)束后，按Bio-Rad產(chǎn)品說(shuō)明，凝膠靠近陰極一側(cè)，硝酸纖維膜(NC膜)靠近陽(yáng)極一側(cè)，置轉(zhuǎn)移緩沖液中(25mmol/LTris、192mmol/LGlycine、20%甲醇)，100V恒壓lh，將蛋白從凝膠電轉(zhuǎn)移至NC膜上，電轉(zhuǎn)移結(jié)束后，取出NC膜，用洗滌液TBST(含mol/TBS、Tween20)室溫洗3次，浸入封閉液(含2%BSA的TBST)中37。C，1h，洗滌液(TBST)室溫洗3次，加荷瘤小鼠抗血清，37t孵育1h，TBST室溫洗膜3次，加入兔抗山羊IgG-HRP二抗(中杉公司)，天津IMAB362特異人源Claudin18.2蛋白，37'C孵育1h，TBST室溫洗膜3次，再用TBS洗3次，NC膜浸入OPD顯色液中，室溫避光顯色10min，蒸餾水沖洗終止反應(yīng)。天津IMAB362特異人源Claudin18.2蛋白</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津IMAB362特異人源Claudin18.2蛋白,人源Claudin18.2蛋白" src="http://tyunfile.71360.com/UploadFile/shchaorui/637123969950605469/9979953.png"></div><p> MNaH2P04，MTris()充分平衡Ni柱，先用含10mmo1咪唑的6M尿素，，()洗脫雜蛋白，再用含250mmo1咪唑的6M尿素，，(pH8,0)洗脫目的蛋白。收集洗脫峰，純化產(chǎn)物透析至2M尿素，，Tris(pH)復(fù)性，終濃度lmg/ml。***透析入Tris(pH)中，PEG8000濃縮后，用Bradford法測(cè)定蛋白濃度。動(dòng)物模型實(shí)驗(yàn)小鼠分組與免疫方案分組將24只BALB/c小鼠分成4組，于小鼠背部皮下按照l(shuí)x^個(gè)/只，每組6只分別接種胃*MFC和胰腺*MPC-83細(xì)胞各2組。每個(gè)腫瘤細(xì)胞接種2組荷瘤小鼠，待成瘤后，按照常規(guī)免疫方法分別注射鹽水。在第4次免疫后10天摘眼球**，測(cè)定血清中抗。免疫方案背部皮下多點(diǎn)注射；rhClaudinl8,2:40ug/只+生理鹽水總體積為200^L/只，分別隔周注射；第4次，使用:40Hg/只+生理鹽水200liL/只，腹腔注射。ELISA法測(cè)定血清抗，收集抗血清，通過(guò)間接ELISA測(cè)定血清抗(:1311出。每個(gè)樣品均設(shè)6個(gè)孔。ELISA實(shí)驗(yàn)方法如下所需溶液的配制a.包被緩沖液碳酸鹽緩沖液取Na2C03:g(終濃度mol/L)，NaHC03:(終濃度mol/L)，純水定容至200mL(),有效期兩周。b.洗滌液含Tween-20的PBS()。c.封閉液含1%BSA的洗滌液。d.稀釋液含BSA的洗滌液。e.底物緩沖液Na2HP04*12H20:g，檸檬酸mL(pH)。f.底物顯色液OPD:8mg。北京高純度人源Claudin18.2蛋白****</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津IMAB362特異人源Claudin18.2蛋白,人源Claudin18.2蛋白" src="http://tyunfile.71360.com/UploadFile/shchaorui/637123969953671875/8173621.png"></div><p> 診斷、***或預(yù)防。因此，在一個(gè)方面，本**技術(shù)涉及一種分離的單克隆抗體(例如，人源化抗體)、或其抗原結(jié)合部分，其與，含有重鏈可變區(qū)，該重鏈可變區(qū)含有CDR1區(qū)、CDR2區(qū)和CDR3區(qū)，其中，CDR1區(qū)、CDR2區(qū)和CDR3區(qū)所含的氨基酸序列分別包含(1)SEQIDNOs：1、4和7；(2)SEQIDNOs：2、4和8；(3)SEQIDNOs：2、4和9；(4)SEQIDNOs：2、5和9；或(5)SEQIDNOs：3、6和8；或與其具有至少80％、85％、90％、95％、98％或99％同一性，其中該抗體或其抗原結(jié)合部分與。在一個(gè)方面，本**技術(shù)的分離的單克隆抗體或其抗原結(jié)合部分包含重鏈可變區(qū)，其所含的氨基酸序列為SEQIDNOs：17、18、19、20、21、22、23、24、25、26、27、28、29、30、31、32、33、34、35、36、37、38、39、40、41、42、43、44、45、46、47、48、或49，或與其具有至少80％、85％、90％、95％、98％或99％同一性，其中該抗體或其抗原結(jié)合部分與。在一個(gè)方面，本**技術(shù)的分離的單克隆抗體或其抗原結(jié)合部分含有輕鏈可變區(qū)，該輕鏈可變區(qū)含有CDR1區(qū)、CDR2區(qū)和CDR3區(qū)，其中，CDR1區(qū)、CDR2區(qū)和CDR3區(qū)所含的氨基酸序列分別為(1)SEQIDNOs：10、13和16；(2)SEQIDNOs：11、13和16；(3)SEQIDNOs：10、14和16；。</p><p> 免疫印跡反應(yīng)SDS-PAGE結(jié)束后，按Bio-Rad產(chǎn)品說(shuō)明，凝膠靠近陰極一側(cè)，硝酸纖維膜(NC膜)靠近陽(yáng)極一側(cè)，置轉(zhuǎn)移緩沖液中(25mmol/LTris、192mmol/LGlycine、20%甲醇)，100V恒壓lh，將蛋白從凝膠電轉(zhuǎn)移至NC膜上，電轉(zhuǎn)移結(jié)束后，取出NC膜，用洗滌液TBST(含mol/LTBS、Tween20)室溫洗3次，浸入封閉液(含2°/。BSA的TBST)中37°C，1h，洗滌液(TBST)室溫洗3次，加山羊抗人，37。C孵育1h，TBST室溫洗膜3次，加入兔抗山羊IgG-HRP二抗，37'C孵育lh，TBST室溫洗膜3次，再用TBS洗3次，NC膜浸入OPD顯色液中，室溫避光顯色10min，蒸餾水沖洗終止反應(yīng)。(3)融合蛋白的純化和復(fù)性取含，5000rpm離心10min,收菌，超聲裂菌；4'C，12000rpm，離心20min,分別收集上清液和沉淀。將沉淀用6mol/L尿素溶液重懸，fC下靜置溶解。將溶解的沉淀和上清分別取樣進(jìn)行SDS-PAGE電泳，對(duì)目的蛋白的表達(dá)形式進(jìn)行分析。在證實(shí)目的蛋白以包涵體的形式表達(dá)后，用Ni-NTA柱親和層析純化所溶解的沉淀(包涵體)。按1g菌體加10mL裂菌緩沖液的比例將菌體重懸，冰浴條件下進(jìn)行超聲裂菌。12000rpm，離心15min，棄上清，1g沉淀加10mL6M尿素，，MTris(pH)。4'C攪拌2h，12000rpm，離心15min，共離心2次，收集上清待用。用6M尿素。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津IMAB362特異人源Claudin18.2蛋白,人源Claudin18.2蛋白" src="http://tyunfile.71360.com/UploadFile/shchaorui/637123969946435547/5682602.png"></div><p> 再用含250mmol咪唑的6M尿素，,(pH8,0)洗脫目的蛋白。收集洗脫峰，純化產(chǎn)物透析至2M尿素，，Tris(pH)復(fù)性，終濃度lmg/ml。***透析入(pH)中，PEG8000濃縮后，用Bradford法測(cè)定蛋白濃度(參見(jiàn)圖2)。三、動(dòng)物模型實(shí)驗(yàn)(1)小鼠分組與免疫方案分組將24只BALB/c小鼠分成4組，于小鼠背部皮下按照口107個(gè)/只，每組6只分別接種胃*MFC和胰腺*MPC-83細(xì)胞各2組。每個(gè)腫瘤細(xì)胞接種2組荷瘤小鼠，待成瘤后，按照常規(guī)免疫方法分別注射鹽水。在第4次免疫后10天摘眼球**，測(cè)定血清中抗。免疫方案背部皮下多點(diǎn)注射；rhClaudinl8,2:40Wg/只+生理鹽水總體積為200txL/只，分別隔周注射；第4次，使用:40ug/只+生理鹽水200wL/只，腹腔注射。(2)￡03八法測(cè)定血清抗(:1&11&在第4次免疫后10天**，收集抗血清，通過(guò)間接ELISA測(cè)定血清抗。每個(gè)樣品均設(shè)6個(gè)孔。ELISA實(shí)驗(yàn)方法如下所需溶液的配制a.包被緩沖液碳酸鹽緩沖液取Na2C03:g(終濃度mol/L)，NaHC03:(終濃度mol/L)，純水定容至200mL()，有效期兩周。b.洗滌液含Tween-20的PBS()。c.封閉液含1%BSA的洗滌液。d.稀釋液含BSA的洗滌液。e.底物緩沖液Na2HP04*12H20:g，檸檬酸mL(pH)。f.底物顯色液OPD:8mg，3%H202:30uL。h.終止液H2S04:lmol/L。北京高純度人源Claudin18.2蛋白****</p><p style="text-indent:25px">天津IMAB362特異人源Claudin18.2蛋白</p><p> 25mmol/LTris、192mmol/LGlycine、20%甲醇)，100V恒壓lh，將蛋白從凝膠電轉(zhuǎn)移至NC膜上，電轉(zhuǎn)移結(jié)束后，取出NC膜，用洗滌液TBST(含mol/LTBS、)室溫洗3次，浸入封閉液(含2%BSA的TBST)中37°C，1h，洗滌液(TBST)室溫洗3次，加山羊抗人(Santacruze公司)，37'C孵育1h，TBST室溫洗膜3次，加入兔抗山羊IgG-HRP二抗(中杉公司)，37。C孵育1h，TBST室溫洗膜3次，再用TBS洗3次，NC膜浸入OPD顯色液中，室溫避光顯色10min，蒸餾水沖洗終止反應(yīng)(參見(jiàn)圖3)。(3)融合蛋白的純化和復(fù)性取含，5000rpm離心10min，收菌，超聲裂菌；4°C,12000rpm，離心20min，分別收集上清液和沉淀。將沉淀用6mol/L尿素溶液重懸，4'C下靜置溶解。將溶解的沉淀和上清分別取樣進(jìn)行SDS-PAGE電泳，對(duì)目的蛋白的表達(dá)形式進(jìn)行分析。在證實(shí)目的蛋白以包涵體的形式表達(dá)后，用Ni-NTA柱親和層析純化所溶解的沉淀(包涵體)。按1g菌體加10mL裂菌緩沖液的比例將菌體重懸，冰浴條件下進(jìn)行超聲裂菌。12000rpm,離心15min，棄上清，1g沉淀加10mL6M尿素，，MTris(pH)。4"C攪拌2h，12000rpm，離心15min，共離心2次，收集上清待用。用6M尿素，MNaH2P04，MTris()充分平衡Ni柱，先用含10mmo1咪唑的6M尿素，,()洗脫雜蛋白。天津IMAB362特異人源Claudin18.2蛋白</p><p style="text-indent:25px">上海朝瑞生物科技有限公司主要經(jīng)營(yíng)范圍是化工，擁有一支專業(yè)技術(shù)團(tuán)隊(duì)和良好的市場(chǎng)口碑。上海朝瑞科技致力于為客戶提供良好的科研技術(shù)服務(wù)，應(yīng)用技術(shù)服務(wù)，科研成果轉(zhuǎn)化，科學(xué)產(chǎn)品銷售，一切以用戶需求為中心，深受廣大客戶的歡迎。公司注重以質(zhì)量為中心，以服務(wù)為理念，秉持誠(chéng)信為本的理念，打造化工良好品牌。上海朝瑞科技憑借創(chuàng)新的產(chǎn)品、專業(yè)的服務(wù)、眾多的成功案例積累起來(lái)的聲譽(yù)和口碑，讓企業(yè)發(fā)展再上新高。</p></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/1114412.html</p></div></div></div></div></div><div id="jifhzbd" class="page-right twoColRt"><div id="f3fctqc" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="j4gdp7o" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" alt="福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></div><p>福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)</p></a></div><div 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