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</span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機: </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="g55gbgb" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="o3l8jiz" class="box"><p><p> 食品安全性毒理學(xué)評價試驗的內(nèi)容包括哪些? </p> <p> <br /> </p> <p> 食品安全性毒理學(xué)評價試驗的四個階段： </p> <p> （1）急性毒性試驗： </p> <p> ①用霍恩氏、幾率單位法或寇氏法測定經(jīng)口LD50，如劑量達10g/kgB.w.，仍不引起動物死亡，則不必測定LD50； </p> <p> ②7天喂養(yǎng)試驗。 </p> <p> （2）蓄積毒性和致突變試驗： </p> <p> ①分別用蓄積系數(shù)法和20天試驗法進行蓄積毒性試驗； </p> <p> ②致突變試驗包括細(xì)菌致突變試驗，微核試驗和骨髓細(xì)胞染色體畸變分析試驗中任一項，顯性致死試驗、睪丸生殖細(xì)胞染色體畸變分析試驗和精子畸形試驗中任一項，以及DNA修復(fù)合成試驗。 </p> <p> （3）亞慢性毒性和代謝試驗： </p> <p> ①亞慢性毒性試驗包括90天喂養(yǎng)試驗、喂養(yǎng)繁殖試驗、喂養(yǎng)致畸試驗和傳統(tǒng)致畸試驗； </p> <p> ②代謝試驗包括胃腸道吸收，測定血液濃度并計算生物半減期和其他動力學(xué)指標(biāo)，主要***和組織中的分布，排泄（尿，上海遺傳毒理學(xué)安全性評價實驗服務(wù)、糞、膽汁）。有條件時可進一步進行代謝產(chǎn)物的分離和鑒定。 </p> <p> （4）慢性毒性試驗：將兩年慢性毒性試驗和致*試驗結(jié)合在一個動物試驗中進行，上海遺傳毒理學(xué)安全性評價實驗服務(wù)。 </p> <p> <br /> 非臨床研究，系指為評價***安全性，上海遺傳毒理學(xué)安全性評價實驗服務(wù)，用實驗系統(tǒng)進行的各種毒性試驗，包括單次給藥的毒性試驗。上海遺傳毒理學(xué)安全性評價實驗服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海遺傳毒理學(xué)安全性評價實驗服務(wù),毒理學(xué)安全性評價" src="http://tyunfile.71360.com/UploadFile/shchaorui/637129699839287110/5826880.png"></div><p> 經(jīng)口半數(shù)致死量），了解受試物的毒性強度、性質(zhì)和可能的靶***，為進一步進行毒性試驗的劑量和毒性觀察指標(biāo)的選擇提供依據(jù)，并根據(jù)LD50進行毒性分級。試驗項目：①霍恩氏法、機率單位法或寇氏法測定經(jīng)口半數(shù)致死量（LD50）；②7天喂養(yǎng)試驗。以上兩個項目分別用兩種性別的小鼠和（或）大鼠進行結(jié)果判定：①LD50或7天喂養(yǎng)試驗的**小有作用劑量小于人的可能攝入量的10倍者，則放棄不再繼續(xù)試驗；②大于10倍者，可進入下一階段試驗。為慎重起見，凡LD50在10倍左右時，應(yīng)進行重復(fù)試驗，或用另一種方法進行驗證。[2]安全毒理學(xué)評價（2）第二階段遺傳毒性試驗考慮原核細(xì)胞與真核細(xì)胞、體內(nèi)試驗和體外試驗相結(jié)合的原則選擇試驗組合，對受試物的遺傳毒性以及是否具有潛在致*作用進行篩選。食品毒理學(xué)中的傳統(tǒng)致畸試驗是限定于受孕動物的觀察，了解受試物是否具有致畸作用。在急性毒性試驗的基礎(chǔ)上，通過30天喂養(yǎng)試驗，進一步了解其毒性作用，觀察對生長發(fā)育的影響，并可初步估計比較大未觀察到有害作用的劑量。1.蓄積毒性試驗①目的：了解受試物在體內(nèi)的蓄積情況。②試驗項目：包括蓄積系數(shù)法和20天試驗法。蓄積系數(shù)法是用兩種性別的大鼠或小鼠，各20只。天津毒理學(xué)安全性評價實驗系統(tǒng)，系指用于毒性試驗的動物、植物、微生物以及***、組織、細(xì)胞、基因等。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海遺傳毒理學(xué)安全性評價實驗服務(wù),毒理學(xué)安全性評價" src="http://tyunfile.71360.com/UploadFile/shchaorui/637129699844843750/4385430.png"></div><p style="text-indent:25px">通常選兔、小型豬，否則應(yīng)闡明合理性。兔每組不低于4只。一般應(yīng)進行相同備皮面積的正常皮膚和破損皮膚局部刺激性試驗。采用同體左右側(cè)自身對比法，將受試物直接涂于備皮處，敷料覆蓋固定。貼敷時間至少4小時。多次給藥皮膚刺激性試驗應(yīng)連續(xù)在同一部位給藥，每次給藥時間相同，給藥期限一般不超過4周。破損皮膚試驗中皮膚破損程度以損傷表皮層為限。在自然光線或全光譜燈光下肉眼觀察皮膚反應(yīng)。根據(jù)受試物的特點和刺激性反應(yīng)情況選擇觀察時間。通常單次給藥皮膚刺激性試驗觀察時間點為去除***后30～60分鐘，24、48和72小時。多次給藥皮膚刺激性試驗，為每次去除***后1小時以及每次給藥前，以及末次貼敷去除***后30～60分鐘，24、48和72小時。如存在持久性損傷，有必要延長觀察期限以評價恢復(fù)情況和時間，延長期一般不超過2周。對出現(xiàn)中度及中度以上皮膚刺激性的動物應(yīng)在觀察期結(jié)束時進行組織病理學(xué)檢查，并提供病理照片。單次給藥皮膚刺激性試驗，計算各組每一時間點皮膚反應(yīng)積分的平均值，按表3進行刺激強度評價。多次給藥皮膚刺激性試驗，首先計算每一觀察時間點各組積分均值，然后計算觀察期限內(nèi)每天每只動物刺激積分均值，按表4進行刺激強度評價。</p><p style="text-indent:25px">銀杏葉提取物膠囊的食用安全性進行毒理學(xué)實驗評價。方法采用小鼠急性經(jīng)口毒性試驗、遺傳毒性試驗（Ames試驗、小鼠骨髓微核試驗、小鼠精子畸形試驗）和30d喂養(yǎng)試驗進行檢測評價。結(jié)果小鼠急性經(jīng)口毒性MTD〉20g／kg·bw，三項遺傳毒性試驗結(jié)果均為陰性。30d喂養(yǎng)試驗未顯示明顯毒性。結(jié)論銀杏葉提取物膠囊急性毒性分級屬無毒級，無遺傳毒性，按推薦量食用是安全的。Objective To assess the edible safety oI Propolis capsule. Nletllotls [l~e toxicity was Oetermmed Dy a- cute toxicity test and thirty day feeding tests. The mutations of Propolis capsule was tested using micronucleus test in bonemarrow cell,sperm shape abnormality test and Ames test. The teratogenicity was also assessed. Results The acute oral MTD in mice was over 20.0 g/kg · bw. The results of micronucles test in bone marrow ceil, sperm shape abnormali- ty, Ames test were negative, without significant differences, i-Conclusion]Ginkgo biloba extract capsule belongs to the actu- al innocuous level no-genetic toxicity,being used for food according to recommending amounts is sMe.藥用輔料系指生產(chǎn)藥品和調(diào)配***時使用的賦形劑和附加劑。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海遺傳毒理學(xué)安全性評價實驗服務(wù),毒理學(xué)安全性評價" src="http://tyunfile.71360.com/UploadFile/shchaorui/637129699834218750/8309701.png"></div>***代謝是指***在吸收過程或進入體循環(huán)后，受腸道菌叢或體內(nèi)酶系統(tǒng)的作用，結(jié)構(gòu)發(fā)生轉(zhuǎn)變的過程稱為代謝或生物轉(zhuǎn)化，其產(chǎn)物為代謝產(chǎn)物。 <p> 影響***代謝的因素1、給藥途徑：代謝與***代謝酶在體內(nèi)的分布以及局部***和組織的血流量有關(guān)。給藥途徑是影響***代謝的重要因素之一。 </p> <p> 2、給藥劑量和劑型：代謝與***代謝酶在體內(nèi)的分布以及局部***和組織以的血流量有關(guān)。給藥途徑是影響***代謝的重要因素之一。 </p> <p> 3、***的光學(xué)異構(gòu)特性的影響：***在體內(nèi)代謝反應(yīng)大多都是酶的反應(yīng)，因此，集體對***的代謝能力主要取決于體內(nèi)各種***代謝酶的活力和數(shù)量。 </p> <p> 4、酶抑或酶促作用：酶***劑是使代謝減慢的物質(zhì)，酶誘導(dǎo)劑是使代謝加快的物質(zhì)。藥理學(xué)是生命科學(xué)的重要組成部分。上海遺傳毒理學(xué)安全性評價實驗服務(wù)</p><p style="text-indent:25px">專題負(fù)責(zé)人系指負(fù)責(zé)組織實施某項研究工作的人員。上海遺傳毒理學(xué)安全性評價實驗服務(wù)</p><p style="text-indent:25px">***組織病理檢查（huotizuzhibinglijiancha）簡稱“活檢”，是采取***組織進行形態(tài)學(xué)檢查是作出疾病診斷的重要方法?；顧z主要用于**和非**性疾病、良性和惡性**的鑒別，判斷惡性**生長、侵犯、轉(zhuǎn)移的程度和范圍，以及對疾病的發(fā)展程度或***反應(yīng)進行觀察等。根據(jù)取活檢時應(yīng)用的器械和方式方法的不同，有切取活檢，是切取部分病變及其鄰近的正常組織；切除活檢，是將全部病變切除送檢；內(nèi)窺鏡活檢，為經(jīng)內(nèi)窺鏡鉗取少許病變組織；針吸活檢，是用穿刺針吸取少量組織。此外，還有刮取、鉆取等方式。然后選取活檢組織制成石蠟切片，在顯微鏡下作組織學(xué)檢查，必要時借助電子顯微鏡或免疫組織化學(xué)法檢查。此外，尚有一種快速活檢，又稱冰凍切片檢查，多用于手術(shù)過程中及時作出判斷，以幫助確定手術(shù)范圍或方式等。上海遺傳毒理學(xué)安全性評價實驗服務(wù)</p><p style="text-indent:25px">上海朝瑞生物科技有限公司總部位于上海市松江區(qū)廣富林路697號昂立大廈2113室，是一家1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗檢測服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級改造產(chǎn)品服務(wù) 9.發(fā)布實驗室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項目團隊招聘13.發(fā)布研究生婚戀 14.發(fā)布項目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù)的公司。上海朝瑞科技作為1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗檢測服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級改造產(chǎn)品服務(wù) 9.發(fā)布實驗室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項目團隊招聘13.發(fā)布研究生婚戀 14.發(fā)布項目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù)的企業(yè)之一，為客戶提供良好的科研技術(shù)服務(wù)，應(yīng)用技術(shù)服務(wù)，科研成果轉(zhuǎn)化，科學(xué)產(chǎn)品銷售。上海朝瑞科技致力于把技術(shù)上的創(chuàng)新展現(xiàn)成對用戶產(chǎn)品上的貼心，為用戶帶來良好體驗。上海朝瑞科技始終關(guān)注化工市場，以敏銳的市場洞察力，實現(xiàn)與客戶的成長共贏。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/1118627.html</p></div></div></div></div></div><div id="qede5ye" class="page-right twoColRt"><div id="yguvqir" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="advhsve" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img 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