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[加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="7fcjqsz" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="bqipmyq" class="box"><p><p> 草害是制約農(nóng)作物產(chǎn)量的主要因子之一。目前,雜草的防治主要依賴化學(xué)除草劑,但其對(duì)環(huán)境的負(fù)面影響日益受到人們的關(guān)注,減少化學(xué)除草劑的使用是未來(lái)農(nóng)業(yè)的發(fā)展趨勢(shì)。發(fā)展微生物除草劑,實(shí)現(xiàn)雜草的生物防治,可以大幅度減少化學(xué)除草劑的使用,實(shí)現(xiàn)農(nóng)業(yè)的可持續(xù)發(fā)展。禾長(zhǎng)蠕孢稗草?；?HelhosporiumgramineumRabenh),HGE對(duì)稗草的致病力強(qiáng),對(duì)水稻安全,是稗草生物防治的潛力菌。為進(jìn)一步提高該菌種的孢子產(chǎn)量和代謝產(chǎn)物產(chǎn)量,采用紫外誘變對(duì)該菌種進(jìn)行了改良，江蘇農(nóng)業(yè)微生物菌種定向改良合成途徑。兩突變菌株均具有良好的遺傳穩(wěn)定性,對(duì)水稻、玉米等作物安全。利用RAPD-PCR方法對(duì)出發(fā)菌株和突變菌株M1和M3進(jìn)行了分子鑒定,初步說(shuō)明突變菌株的遺傳物質(zhì)發(fā)生了改變。對(duì)出發(fā)菌株和突變菌株的固體發(fā)酵和液體發(fā)酵工藝進(jìn)行了初步研究，江蘇農(nóng)業(yè)微生物菌種定向改良合成途徑,確定了相應(yīng)的比較好發(fā)酵條件。其中固體發(fā)酵的比較好條件是稗草基質(zhì)培養(yǎng)基中添加生長(zhǎng)因子③,以2菌塊/克稗草干物質(zhì)接種,27℃培養(yǎng)箱中黑暗培養(yǎng)10天，江蘇農(nóng)業(yè)微生物菌種定向改良合成途徑。突變菌株M1菌絲體產(chǎn)量的比較好發(fā)酵條件組合為:3號(hào)培養(yǎng)基,初始pH值為7,發(fā)酵溫度為24℃,150r/min振蕩培養(yǎng)6d;***產(chǎn)量的比較好發(fā)酵條件組合為:4號(hào)培養(yǎng)基,初始pH值為7,發(fā)酵溫度為26℃,靜置培養(yǎng)14d。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇農(nóng)業(yè)微生物菌種定向改良合成途徑" src="http://tyunfile.71360.com/UploadFile/shchaorui/637190230318701172/9190410.png"></div><p> 綠色農(nóng)業(yè)的提出,有效微生物(EffectiveMicranims,EM)制劑越來(lái)越受到人們的重視并被廣泛應(yīng)用于畜牧生產(chǎn)當(dāng)中,以避免不合理使用***對(duì)動(dòng)物以及人類造成的負(fù)面影響。在以往的研究中往往是在某一個(gè)較短的時(shí)間段對(duì)動(dòng)物飼喂EM制劑,有些試驗(yàn)并沒(méi)有注明在動(dòng)物飼養(yǎng)的飼料中是不是含有***等添加劑。在此情況下研究EM制劑的營(yíng)養(yǎng)和免疫調(diào)節(jié)作用等,很難消除***對(duì)動(dòng)物造成的影響，本研究選取400只大小一致的健康海蘭褐蛋雞初生雛,從一日齡開(kāi)始飼喂經(jīng)EM制劑發(fā)酵過(guò)的飼料,直到飼養(yǎng)試驗(yàn)結(jié)束。在整個(gè)飼養(yǎng)過(guò)程中未使用任何***和化學(xué)***。然后對(duì)蛋雞的生長(zhǎng)性能和免疫功能進(jìn)行定時(shí)檢測(cè),得出數(shù)據(jù)并總結(jié)整理,試驗(yàn)一日糧中添加EM制劑對(duì)蛋雞免疫水平的影響從體液免疫和細(xì)胞免疫兩方面綜合研究了EM制劑對(duì)海蘭蛋雞免疫水平的影響。結(jié)果表明,隨著日糧中EM制劑發(fā)酵飼料比例的提高,海蘭蛋雞的***也隨之增強(qiáng),當(dāng)發(fā)酵飼料比例達(dá)到40%左右時(shí),海蘭蛋雞的免疫能力***增強(qiáng)。試驗(yàn)二飼喂EM制劑對(duì)蛋雞免疫***的影響從蛋雞免疫***的**切片可以看出,各組試驗(yàn)動(dòng)物生理狀況良好,沒(méi)有發(fā)生病理變化,**內(nèi)的淋巴細(xì)胞數(shù)量也沒(méi)有***差異。同時(shí),各試驗(yàn)組與對(duì)照組之間免疫***重量沒(méi)有***差異。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇農(nóng)業(yè)微生物菌種定向改良合成途徑" src="http://tyunfile.71360.com/UploadFile/shchaorui/637190230317294922/2104477.png"></div><p> 從常溫研磨、蛋白酶K和R nase的加入對(duì)傳統(tǒng)CTAB法進(jìn)行改良,并結(jié)合試劑盒法,提出了一種***提取高溫大曲微生物總DNA的方法。該方法獲得的總基因片段大小約21Kb;A260/A280=1.878;A260/A230=1.706;PCR反應(yīng)***物少,可直接用于16S rDNA的擴(kuò)增;并通過(guò)構(gòu)建克隆文庫(kù)技術(shù)和變性梯度凝膠電泳(DGGE)分析后發(fā)現(xiàn)高溫大曲中細(xì)菌多樣性豐富,能一定程度上反映微生物群落結(jié)構(gòu)和組成。研究結(jié)果表明該方法提取的DNA適用于芝麻香高溫大曲中微生物的分子生態(tài)學(xué)研究。 </p> <p> <br /> </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇農(nóng)業(yè)微生物菌種定向改良合成途徑" src="http://tyunfile.71360.com/UploadFile/shchaorui/637190230317539062/7830398.png"></div></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/132293.html</p></div></div></div></div></div><div id="3gdah7b" class="page-right twoColRt"><div id="gl7wtax" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="dxzbykm" class="recomPic likePro"><a 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