北京常規(guī)蛋白Western Blot免疫印跡服務(wù) **** 上海朝瑞生物科技供應(yīng)-b2b網(wǎng)站_b2b電子商務(wù)平臺【買產(chǎn)品】-上?？谄蚴称酚邢薰?/title><meta name="description" content="Westernblotting堪稱蛋白質(zhì)研究中的經(jīng)典方法。學(xué)生物的幾乎無人不曉，即使沒親手做過，也清楚其原理。經(jīng)典果然是經(jīng)典，30年來其操作步驟幾乎沒變過，依然是跑膠-轉(zhuǎn)膜-封閉-孵育-檢測。近兩年，北京常規(guī)蛋白WesternBlot免疫印"><meta name="keywords" content="北京常規(guī)蛋白Western Blot免疫印跡服務(wù),western blot"><link rel="canonical" href="http://www.cdcfah.com/"/><meta name="applicable-device" content="pc"><link rel="shortcut icon" href="/static/new_net_01/images/favicon.ico"><link rel="stylesheet" href="/static/new_net_01/css/slick.css" /><link rel="stylesheet" href="/static/new_net_01/css/addnew.css"><link rel="stylesheet" href="/static/new_net_01/css/sidenav.css"><link rel="stylesheet" href="/static/new_net_01/css/style.css" /><link rel="stylesheet" href="/static/new_net_01/css/tab.css"><link rel="stylesheet" href="/static/new_net_01/css/404.css"> <meta name="baidu-site-verification" content="OBVzrC9aaQ" /> 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clearfix"><h1>北京常規(guī)蛋白Western Blot免疫印跡服務(wù) **** 上海朝瑞生物科技供應(yīng)</h1><div id="8fgizlh" class="proBig"><div id="3j2k8u7" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637115400030400391/2584407.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="mbfgnjk" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637115400030400391/2584407.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="2sn227u" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>北京常規(guī)蛋白Western Blot免疫印跡服務(wù),western blot</p><p><span>***更新：</span>2020-09-18 05:07:01</p><p><span id="click" >瀏覽次數(shù)：1次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="9st79pn" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="8l3mijk" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="kxvw3xi" class="box"><p><p> Western blotting堪稱蛋白質(zhì)研究中的經(jīng)典方法。學(xué)生物的幾乎無人不曉，即使沒親手做過，也清楚其原理。經(jīng)典果然是經(jīng)典，30年來其操作步驟幾乎沒變過，依然是跑膠-轉(zhuǎn)膜-封閉-孵育-檢測。近兩年，北京常規(guī)蛋白Western Blot免疫印跡服務(wù)，各大公司陸續(xù)推出了Western blotting方面的新儀器，如新型轉(zhuǎn)印系統(tǒng)，北京常規(guī)蛋白Western Blot免疫印跡服務(wù)，確實能省時省力不少，但仍然不能完全克服研究人員在面臨的挑戰(zhàn)。Western </p> <p> blotting仍存在重復(fù)性差、實驗耗時長、無法準(zhǔn)確定量等問題。 </p> <p> 近日，美國proteinsimple公司推出了一種Simple Western?分析，徹底改變了整個Western blot。此分析在一臺名為Simon的儀器上開展，Simon將所有實驗步驟自動化，北京常規(guī)蛋白Western Blot免疫印跡服務(wù)，包括蛋白上樣和分離、免疫印跡、洗滌、檢測以及數(shù)據(jù)的定量分析，讓研究人員從繁重的勞動中解放出來，同時避免了可能影響實驗重復(fù)性的人為因素。北京常規(guī)蛋白Western Blot免疫印跡服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="北京常規(guī)蛋白Western Blot免疫印跡服務(wù),western blot" src="http://tyunfile.71360.com/UploadFile/shchaorui/637115400030400391/2584407.png"></div><p> 轉(zhuǎn)膜完畢后，立即把蛋白膜放置到預(yù)先準(zhǔn)備好的Western洗滌液(P0023C)中，漂洗1-2分鐘，以洗去膜上的轉(zhuǎn)膜液。從轉(zhuǎn)膜完畢后所有的步驟，一定要注意膜的保濕，避免膜的干燥，否則極易產(chǎn)生較高的背景。用微型臺式真空泵或滴管等吸盡封閉液，立即加入稀釋好的一抗，室溫或4℃在側(cè)擺搖床上緩慢搖動孵育一小時。如果一抗孵育一小時效果不佳，可以4℃緩慢搖動孵育過夜?；蚋鼡?jù)抗體的說明選擇適當(dāng)?shù)姆跤郎囟群蜁r間。微型臺式真空泵或滴管等吸盡洗滌液，立即加入稀釋好的二抗，室溫或4℃在側(cè)擺搖床上緩慢搖動孵育一小時。回收二抗。加入Western洗滌液，在側(cè)擺搖床上緩慢搖動洗滌5-10分鐘。吸盡洗滌液后，再加入洗滌液洗滌5-10分鐘。共洗滌3次。如果結(jié)果背景較高可以適當(dāng)延長洗滌時間并增加洗滌次數(shù)。使用 </p> <p> ECL化學(xué)發(fā)光試劑來檢測蛋白。壓片可以采用**的壓片暗盒進(jìn)行。洗片時可以使用X光片自動洗片機(jī)。如果沒有自動洗片機(jī)，可以用顯影定影試劑盒自行配制顯影液和定影液進(jìn)行手工洗片。X光片推薦選用柯達(dá)原裝的生物實驗**柯達(dá)X-OMAT BT膠片。北京常規(guī)蛋白Western Blot免疫印跡服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="北京常規(guī)蛋白Western Blot免疫印跡服務(wù),western blot" src="http://tyunfile.71360.com/UploadFile/shchaorui/637115400028710938/8932273.png"></div><p> 全自動western blot無需凝膠、無需轉(zhuǎn)印，放入您的樣品，按個按鈕，然后就沒事了。首先，將樣品與Simple </p> <p> Western樣品緩沖液混合，至終濃度1 μg/μL，還原并變性，然后將制備好的樣品、一抗和二抗、化學(xué)發(fā)光底物分配到384孔分析板的指定孔中。之后，將Simple </p> <p> Western分析緩沖液、毛細(xì)管和制備好的分析板放入Simon內(nèi)，它自動開展所有分析步驟。當(dāng)?shù)鞍籽刂?xì)管內(nèi)一個堆積和分離基質(zhì)遷移時，它們分離。分離后的蛋白固定到毛細(xì)管壁上。之后利用一抗鑒定目標(biāo)蛋白，用HRP結(jié)合的二抗和化學(xué)發(fā)光底物檢測蛋白。系統(tǒng)自動報告檢測蛋白的分子量和信號。每次實驗可同時分析**多12個樣品，結(jié)果可在3-5小時內(nèi)獲得。15-150 kDa的目標(biāo)蛋白均可檢測。軟件報告每個檢測蛋白的分子量、面積、面積百分比和信噪比。對于傳統(tǒng)的Western blot而言，結(jié)果的重復(fù)性一直是個挑戰(zhàn)，因為缺乏標(biāo)準(zhǔn)化的操作，且多個操作步驟引入了實驗可變性。而Simple Western分析是完全自動化的，因此結(jié)果的重復(fù)性更好。整體的定量也**改善了，因為省去了轉(zhuǎn)印步驟，從而避免了蛋白轉(zhuǎn)移的不一致。 </p><p style="text-indent:25px">全自動單細(xì)胞western blot檢測分析原理：全自動單細(xì)胞western blot分析是單細(xì)胞水平，蛋白質(zhì)表達(dá)定量分析系統(tǒng)。系統(tǒng)采用**的微流控western blot芯片，通過單細(xì)胞微孔設(shè)計，采集單細(xì)胞，然后原位裂解細(xì)胞，釋放蛋白，進(jìn)行蛋白質(zhì)電泳，將不同分子量蛋白進(jìn)行分離，提高免疫學(xué)檢測特異性。之后，采用**技術(shù)進(jìn)行蛋白質(zhì)原位捕獲，使用western blot驗證抗體及熒光標(biāo)記二抗直接雜交，掃描儀進(jìn)行芯片掃描后，分析軟件對掃描結(jié)果進(jìn)行深度定量分析。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="北京常規(guī)蛋白Western Blot免疫印跡服務(wù),western blot" src="http://tyunfile.71360.com/UploadFile/shchaorui/637115400028710938/2976777.png"></div><p> (1) SDS-PAGE凝膠配制 </p> <p> SDS-PAGE凝膠可以使用商業(yè)生產(chǎn)的SDS-PAGE凝膠配制試劑盒。 </p> <p> (2) 樣品處理：在收集的蛋白樣品中加入適量濃縮的SDS-PAGE蛋白上樣緩沖液。例如2X或5X的SDS-PAGE蛋白上樣緩沖液。使用5X的SDS-PAGE蛋白上樣緩沖液可以減小上樣體積，在相同體積的上樣孔內(nèi)可以上樣更多的蛋白樣品。5X的SDS-PAGE蛋白上樣緩沖液可以參考相關(guān)文獻(xiàn)資料配制100℃或沸水浴加熱3-5分鐘，以充分變性蛋白。 </p> <p> (3) 上樣與電泳：冷卻到室溫后，把蛋白樣品直接上樣到SDS-PAGE膠加樣孔內(nèi)即可。為了便于觀察電泳效果和轉(zhuǎn)膜效果，以及判斷蛋白分子量大小，比較好使用預(yù)染蛋白質(zhì)分子量標(biāo)準(zhǔn)(P0066)。電泳時通常推薦在上層膠時使用低電壓恒壓電泳，而在溴酚藍(lán)進(jìn)入下層膠時使用高電壓恒壓電泳。對于Bio-Rad的標(biāo)準(zhǔn)電泳裝置或類似電泳裝置，低電壓可以設(shè)置在80-100V，高電壓可以設(shè)置在120V左右。SDS-PAGE可以采用普通的電泳儀就可以滿足要求。通常電泳時溴酚藍(lán)到達(dá)膠的底端處附近即可停止電泳，或者可以根據(jù)預(yù)染蛋白質(zhì)分子量標(biāo)準(zhǔn)的電泳情況，預(yù)計目的蛋白已經(jīng)被適當(dāng)分離后即可停止電泳。上海常規(guī)蛋白Western Blot檢測服務(wù)</p><p style="text-indent:25px">北京常規(guī)蛋白Western Blot免疫印跡服務(wù)</p><p> 1.為SDS—聚丙烯酰胺凝膠電泳（SDS-PAGE）?？乖鹊鞍讟悠方?jīng)SDS處理后帶陰電荷，在聚丙烯酰膪凝膠中從陰極向陽極泳動，分子量越小，泳動速度就越快。此階段分離效果肉眼不可見（只有在染色后才顯出電泳區(qū)帶）。2.電轉(zhuǎn)移。將在凝膠中已經(jīng)分離的條帶轉(zhuǎn)移至硝酸纖維素膜上，選用低電壓（100V）和大電流（1～2A），通電45min轉(zhuǎn)移即可完成。此階段分離的蛋白質(zhì)條帶肉眼仍不可見。3.酶免疫定位。將印有蛋白質(zhì)條帶的硝酸纖維素膜（相當(dāng)于包被了抗原的固相載體）依次與特異性抗體和酶標(biāo)第二抗體作用后，加入能形成不溶性顯色物的酶反應(yīng)底物，使區(qū)帶染色。常用的HRP底物為3，3，—二氨基聯(lián)苯胺（呈棕色）和4-氯-1-萘酚（呈藍(lán)紫色）。陽性反應(yīng)的條帶清晰可辨，并可根據(jù)SDS-PAGE時加人的分子量標(biāo)準(zhǔn)，確定各組分的分子量。本法綜合了SDS-PAGE的辨力和ELISA法的高特異性和敏感性，是一個有效的分析手段，不僅廣泛應(yīng)用于分析抗原組分及其免疫活性，并可用于疾病的診斷。在**中此法作為確診試驗?？乖?jīng)電泳轉(zhuǎn)移在硝酸纖維素膜上后，將膜切成小條，配合酶標(biāo)抗體及顯色底物制成的試劑盒可方便地在實驗室中供檢測用。北京常規(guī)蛋白Western Blot免疫印跡服務(wù)</p><p style="text-indent:25px">上海朝瑞生物科技有限公司總部位于上海市松江區(qū)廣富林路697號昂立大廈2113室，是一家1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗檢測服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級改造產(chǎn)品服務(wù) 9.發(fā)布實驗室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項目團(tuán)隊招聘13.發(fā)布研究生婚戀 14.發(fā)布項目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù)的公司。上海朝瑞科技擁有一支經(jīng)驗豐富、技術(shù)創(chuàng)新的專業(yè)研發(fā)團(tuán)隊，以高度的專注和執(zhí)著為客戶提供科研技術(shù)服務(wù)，應(yīng)用技術(shù)服務(wù)，科研成果轉(zhuǎn)化，科學(xué)產(chǎn)品銷售。上海朝瑞科技致力于把技術(shù)上的創(chuàng)新展現(xiàn)成對用戶產(chǎn)品上的貼心，為用戶帶來良好體驗。上海朝瑞科技始終關(guān)注自身，在風(fēng)云變化的時代，對自身的建設(shè)毫不懈怠，高度的專注與執(zhí)著使上海朝瑞科技在行業(yè)的從容而自信。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/1324547.html</p></div></div></div></div></div><div id="c2f29du" class="page-right twoColRt"><div id="8m3m8xt" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="yb8mdof" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" alt="福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></div><p>福建ZF配件哪家強(qiáng) 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