上海食品安全熒光定量PCR技術(shù)服務(wù) 歡迎來(lái)電上海朝瑞生物科技供應(yīng)-b2b網(wǎng)站_b2b電子商務(wù)平臺(tái)【買產(chǎn)品】-上?？谄蚴称酚邢薰?/title><meta name="description" content="純度檢測(cè) RNA溶液的A260/A280的比值即為RNA純度，上海食品安全熒光定量PCR技術(shù)服務(wù)，比值范圍1.8到2.1。變性瓊脂糖凝膠電泳測(cè)定制膠 &emsp"><meta name="keywords" content="上海食品安全熒光定量PCR技術(shù)服務(wù),熒光定量PCR"><link rel="canonical" href="http://www.cdcfah.com/"/><meta name="applicable-device" content="pc"><link rel="shortcut icon" href="/static/new_net_01/images/favicon.ico"><link rel="stylesheet" href="/static/new_net_01/css/slick.css" /><link rel="stylesheet" href="/static/new_net_01/css/addnew.css"><link rel="stylesheet" href="/static/new_net_01/css/sidenav.css"><link rel="stylesheet" href="/static/new_net_01/css/style.css" /><link rel="stylesheet" href="/static/new_net_01/css/tab.css"><link rel="stylesheet" href="/static/new_net_01/css/404.css"> <meta name="baidu-site-verification" content="OBVzrC9aaQ" /> <meta http-equiv="Cache-Control" content="no-transform" /> <meta 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href="http://www.cdcfah.com/cp/">產(chǎn)品供應(yīng)</a>?<a href="http://www.cdcfah.com/cp/second-_nongye/">農(nóng)業(yè)</a>?<a href="http://www.cdcfah.com/cp/third-nongye_nlmyxmhz/">農(nóng)林牧漁項(xiàng)目合作</a>?上海食品安全熒光定量PCR技術(shù)服務(wù) 歡迎來(lái)電上海朝瑞生物科技供應(yīng)</p><div id="fu32qwj" class="leftbox clearfix"><h1>上海食品安全熒光定量PCR技術(shù)服務(wù) 歡迎來(lái)電上海朝瑞生物科技供應(yīng)</h1><div id="kei2taw" class="proBig"><div id="kerdfm7" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253043593750/2359026.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="8nprfmt" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253043593750/2359026.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="kzlnzgx" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>上海食品安全熒光定量PCR技術(shù)服務(wù),熒光定量PCR</p><p><span>***更新：</span>2020-09-19 13:03:03</p><p><span id="click" >瀏覽次數(shù)：1次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="s3aspgs" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="87rtpw3" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="7jqnzgn" class="box"><p><p> 純度檢測(cè) </p> <p> RNA溶液的A260/A280的比值即為RNA純度，上海食品安全熒光定量PCR技術(shù)服務(wù)，比值范圍1.8到2.1。 </p> <p> 變性瓊脂糖凝膠電泳測(cè)定 </p> <p> 制膠 </p> <p> 1g瓊脂糖溶于72ml水中，冷卻至60℃，10 ml的10× MOPS電泳緩沖液和18 ml的37% 甲醛溶液(12.3 M)。 </p> <p> 灌制凝膠板，預(yù)留加樣孔至少可以加入25 μl溶液。膠凝后取下梳子，將凝膠板放入電泳槽內(nèi)，上海食品安全熒光定量PCR技術(shù)服務(wù)，加足量的1×MOPS電泳緩沖液至覆蓋膠面幾個(gè)毫米。 </p> <p> 準(zhǔn)備RNA樣品 </p> <p> 取3μgRNA，加3倍體積的甲醛上樣染液，加EB于甲醛上樣染液中至終濃度為10μg/ml。加熱至70℃孵育15分鐘使樣品變性。 </p> <p> 電泳 </p> <p> 上樣前凝膠須預(yù)電泳5min，隨后將樣品加入上樣孔，上海食品安全熒光定量PCR技術(shù)服務(wù)。5–6V/cm電壓下2h，電泳至溴酚蘭指示劑進(jìn)膠至少2–3cm。 </p> <p> 紫外透射光下觀察并拍照上海食品安全熒光定量PCR技術(shù)服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海食品安全熒光定量PCR技術(shù)服務(wù),熒光定量PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253043593750/2359026.png"></div><p> Scienceladder是讓知識(shí)、技術(shù)、專長(zhǎng)、成果、資源流淌起來(lái)的平臺(tái)！ Scienceladder是聚天下技術(shù)服務(wù)天下的平臺(tái)！ Scienceladder是你的知識(shí)、技術(shù)、專長(zhǎng)、成果實(shí)現(xiàn)價(jià)值轉(zhuǎn)化的平臺(tái)! 發(fā)布你的技術(shù)、成果、專長(zhǎng)，資源讓需求者得到幫助！發(fā)布你的需求，讓供給者幫助你！為科學(xué)做點(diǎn)事、為科學(xué)家做點(diǎn)事、為技術(shù)推廣做點(diǎn)事！ </p> <p> 歡迎來(lái)到上海朝瑞生物科技有限公司網(wǎng)站，聯(lián)系人是仲先生。 </p> <p> 主要經(jīng)營(yíng)科研技術(shù)服務(wù)|應(yīng)用技術(shù)服務(wù)|科研成果轉(zhuǎn)化|科學(xué)產(chǎn)品銷售。 </p> <p> <br /> 上海食品安全熒光定量PCR技術(shù)服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海食品安全熒光定量PCR技術(shù)服務(wù),熒光定量PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253049667969/2728037.png"></div><p style="text-indent:25px">有關(guān)轉(zhuǎn)基因及其產(chǎn)品的安全性的爭(zhēng)論在國(guó)內(nèi)外愈演愈烈，各國(guó)**紛紛出臺(tái)一系列的政策、法規(guī)，要求對(duì)轉(zhuǎn)基因作物進(jìn)行標(biāo)識(shí)，有些國(guó)家對(duì)轉(zhuǎn)基因的比較低含量做了限定，其閾值大小從1-5％不等。這就要求必須對(duì)轉(zhuǎn)基因及其產(chǎn)品進(jìn)行檢測(cè)。常規(guī)的檢測(cè)方法多采用PCR法，如PCR-凝膠電泳、PCR-ELISA等。這些方法普遍存在著PCR產(chǎn)物污染、特異性不高及速度慢等問題。熒光PCR技術(shù)是一種新近發(fā)展起來(lái)的檢測(cè)技術(shù)，它的應(yīng)用將從根本上解決這些問題。 1.收集了國(guó)內(nèi)外商品化的轉(zhuǎn)基因作物品種，并通過查閱有關(guān)文獻(xiàn)及網(wǎng)站初步確定了各種作物的轉(zhuǎn)基因構(gòu)建圖； 2．于反應(yīng)體系中引入U(xiǎn)NG去污染酶，建立了無(wú)污染熒光PCR擴(kuò)增體系； 3．以植物內(nèi)源基因18SrRNA為靶標(biāo)，設(shè)計(jì)了特異性引物和熒光探針，建立了以18SrRNA基因的擴(kuò)增與否來(lái)判斷核酸提取質(zhì)量好壞的方法； 4．以FAM熒光素為標(biāo)記，建立了以35S、NOS、NPTⅡ、Pat、Bar、FMV和CryⅢa為篩選目標(biāo)的轉(zhuǎn)基因農(nóng)作物熒光PCR定性檢測(cè)體系； 5．分別以FAM和TET兩種熒光素標(biāo)記外源基因和內(nèi)源基因，建立了轉(zhuǎn)基因大豆及轉(zhuǎn)基因玉米的熒光定量PCR檢測(cè)體系。</p><p style="text-indent:25px">實(shí)時(shí)熒光定量PCR技術(shù)原理將熒光基團(tuán)加入到PCR反應(yīng)體系中，通過不斷累積熒光信號(hào)從而使對(duì)PCR全程的實(shí)時(shí)監(jiān)測(cè)實(shí)現(xiàn)，再根據(jù)標(biāo)準(zhǔn)曲線定量分析未知模板的方法，即是熒光定量PCR技術(shù)。在實(shí)時(shí)熒光定量PCR中，實(shí)時(shí)檢測(cè)全程PCR擴(kuò)增過程，按照反應(yīng)時(shí)間和熒光信號(hào)的變化能夠?qū)⒁粭l曲線繪制成。混合模板DNA和有熒光素的Taqman探針，遵守聚合酶鏈反應(yīng)規(guī)律使高溫變性，低溫復(fù)性，適溫延伸的熱循環(huán)完成，切斷和模板DNA互補(bǔ)配對(duì)的Taqman探針，熒光素在反應(yīng)體系中游離。熒光在特定光激發(fā)下發(fā)出，被擴(kuò)增的目的基因片段隨著循環(huán)次數(shù)的增加呈級(jí)增長(zhǎng)，Ct值根據(jù)實(shí)時(shí)檢測(cè)與之對(duì)應(yīng)的隨擴(kuò)增而變化熒光信號(hào)強(qiáng)度求取出來(lái)，同時(shí)對(duì)照多個(gè)已知模板濃度的標(biāo)準(zhǔn)品，就能夠使待測(cè)標(biāo)本目的基因的拷貝數(shù)得出。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海食品安全熒光定量PCR技術(shù)服務(wù),熒光定量PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253043593750/2901163.png"></div><p style="text-indent:25px">溫度設(shè)置：在設(shè)置熒光 PCR 程序時(shí)，要仔細(xì)核對(duì)程序中的目標(biāo)溫度、恒溫時(shí)間和循環(huán)次數(shù)等參數(shù)，參數(shù)設(shè)置不正確將直接影響 PCR 檢測(cè)結(jié)果。延伸過程中要設(shè)置讀取收集熒光信號(hào)，如不設(shè)置收集熒光，將無(wú)法保存試驗(yàn)數(shù)據(jù)，無(wú)法判定檢測(cè)結(jié)果，造成試驗(yàn)失敗操作規(guī)范：在對(duì)樣品進(jìn)行核酸提取和實(shí)時(shí)熒光擴(kuò)增時(shí)要規(guī)范操作，防止樣品的交叉污染、酶的降解、假陽(yáng)性的出現(xiàn) 離心管、***頭和 PCR 管均需要高壓滅菌或使用商品化的無(wú) 污染的離心管、***頭和PCR 管。核酸提取結(jié)束后應(yīng)立即進(jìn)行儀器擴(kuò)增，提取的核酸不可長(zhǎng)時(shí)間置于室溫，易受空氣中酶的降解，短期可保存于－20 ℃冰箱，長(zhǎng)期應(yīng)保存于－70 ℃冰箱上海食品安全熒光定量PCR技術(shù)服務(wù)</p><p style="text-indent:25px">上海食品安全熒光定量PCR技術(shù)服務(wù)</p><p> 實(shí)時(shí)熒光定量常用Taqman探針法和SYBR Green I法兩類。 </p> <p> 1.TaqMan探針法 </p> <p> TaqMan 探針法是具有高度特異性的定量PCR技術(shù)。它的工作原理為有一對(duì) PCR 引物和一條探針存在于PCR 反應(yīng)體系中，有熒光淬滅基團(tuán)存在于3′ </p> <p> 端標(biāo)記，有報(bào)告基團(tuán)存在于探針的5′ 端標(biāo)記，探針*和模板特異結(jié)合，兩條引物之間是其結(jié)合點(diǎn)。因此信號(hào)儀器搜集不到，伴隨反應(yīng)的進(jìn)展，探針在外切核酸酶的活性的作用下被切斷，從而造成淬滅基團(tuán)不能吸收基團(tuán)的熒光能量，從而使熒光信號(hào)產(chǎn)生，所以模板 DNA 的拷貝數(shù)取決于信號(hào)的強(qiáng)度。 </p> <p> 2.SYBR Green I法 </p> <p> SYBR Green I為一種具有綠色激發(fā)波長(zhǎng)的染料，其發(fā)射波長(zhǎng)比較大約為 520 nm，比較大吸收波長(zhǎng)約為 497 nm，能夠結(jié)合所有的雙螺旋小溝區(qū)域。由于處于游離狀態(tài)，SYBR Green I發(fā)出的熒光較弱，然而其結(jié)合雙鏈 DNA之后，就會(huì)使得熒光**增強(qiáng)，熒光信號(hào)的增加完全同步PCR 產(chǎn)物的增加。上海食品安全熒光定量PCR技術(shù)服務(wù)</p><p style="text-indent:25px">上海朝瑞生物科技有限公司致力于化工，是一家服務(wù)型的公司。公司業(yè)務(wù)涵蓋科研技術(shù)服務(wù)，應(yīng)用技術(shù)服務(wù)，科研成果轉(zhuǎn)化，科學(xué)產(chǎn)品銷售等，價(jià)格合理，品質(zhì)有保證。公司秉持誠(chéng)信為本的經(jīng)營(yíng)理念，在化工深耕多年，以技術(shù)為先導(dǎo)，以自主產(chǎn)品為重點(diǎn)，發(fā)揮人才優(yōu)勢(shì)，打造化工良好品牌。上海朝瑞科技立足于全國(guó)市場(chǎng)，依托強(qiáng)大的研發(fā)實(shí)力，融合前沿的技術(shù)理念，飛快響應(yīng)客戶的變化需求。</p></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/1344016.html</p></div></div></div></div></div><div id="fpmykgs" class="page-right twoColRt"><div id="pe89n8y" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="dctqxjj" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img 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