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</span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="hbfzbxb" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="dnrvrvh" class="box"><p><p> 研究?jī)?nèi)容：乳酸乳球菌是廣泛應(yīng)用于食品工業(yè)的一類重要工業(yè)菌株，它所產(chǎn)生的小分子多肽--乳鏈菌肽不僅是一種***、***的天然食品防腐劑，也是研究蛋白質(zhì)分子結(jié)構(gòu)與功能及翻譯后調(diào)控的理想材料。本組主要致力于乳鏈菌肽生物合成基因的克隆、表達(dá)及乳鏈菌肽分子結(jié)構(gòu)與功能關(guān)系的研究及其乳鏈菌肽的大規(guī)模工業(yè)化生產(chǎn)和產(chǎn)品的應(yīng)用開(kāi)發(fā)，并深入研究其產(chǎn)生菌乳酸乳球菌的分子遺傳學(xué)。 </p> <p> 承擔(dān)課題：國(guó)家"九五"攻關(guān)項(xiàng)目"特種保鮮劑-乳鏈菌肽"；國(guó)家自然科學(xué)基金面上項(xiàng)目"一個(gè)新***多肽生物合成基因的研究"；院重點(diǎn)項(xiàng)目"乳酸菌資源、遺傳基礎(chǔ)及重要產(chǎn)物應(yīng)用研究"。 </p> <p> 研究進(jìn)展：1、圓滿完成國(guó)家"九五"攻關(guān)任務(wù)。實(shí)現(xiàn)乳鏈菌肽在20噸發(fā)酵罐規(guī)模的工業(yè)化生產(chǎn)，通過(guò)中科院主持的**鑒定和科技部生物工程中心**的**驗(yàn)收，江蘇冶金微生物菌種定向改良實(shí)驗(yàn)服務(wù)。該項(xiàng)目已經(jīng)轉(zhuǎn)讓，轉(zhuǎn)讓總經(jīng)費(fèi)700萬(wàn)元，已到位50萬(wàn)元。2，江蘇冶金微生物菌種定向改良實(shí)驗(yàn)服務(wù)、在乳酸乳球菌的一個(gè)大質(zhì)粒上，發(fā)現(xiàn)和鑒定了對(duì)乳鏈菌肽具有抗性的決定子，正在將其用作選擇性標(biāo)記構(gòu)建成乳酸乳球菌食品級(jí)載體。3，江蘇冶金微生物菌種定向改良實(shí)驗(yàn)服務(wù)、從嗜酸乳桿菌中分離到一個(gè)廣譜的***多肽AP311，并對(duì)其性質(zhì)進(jìn)行了研究。 </p> <p> 主要成果：本年度發(fā)表學(xué)術(shù)論文2篇；參與編寫專著1部；申請(qǐng)發(fā)明專利1項(xiàng)。 </p> <p> <br /> 尤其針對(duì)近年來(lái)圍繞其開(kāi)展的組學(xué)研究進(jìn)展進(jìn)行詳細(xì)闡述。江蘇冶金微生物菌種定向改良實(shí)驗(yàn)服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇冶金微生物菌種定向改良實(shí)驗(yàn)服務(wù),微生物菌種定向改良" src="http://tyunfile.71360.com/UploadFile/shchaorui/637190230318886718/8257103.png"></div><p> 并對(duì)其進(jìn)行五輪基因組重排，**終獲得一株維吉尼亞霉素產(chǎn)量穩(wěn)定的重組子G5-103，產(chǎn)量約為251mg/L，比親本UV-1150及野生株提高.3、基因組重排技術(shù)存的問(wèn)題與展望目前，基因組重排技術(shù)面臨很多瓶頸，其中融合子的高通量篩選是**難攻克的環(huán)節(jié)，現(xiàn)尚無(wú)快速***的篩選方法，需要研究者借助已有知識(shí)及技術(shù)深入挖掘.另外，由于跨種屬親本之間同源性較差，重組概率較低，因此基因組重排技術(shù)很少用于不同種親本的融合.基因組重排技術(shù)自2002年問(wèn)世以來(lái)便受到人們的***關(guān)注，并應(yīng)用在各種微生物菌種改良中，取得了巨大的經(jīng)濟(jì)及社會(huì)效益.隨著自身技術(shù)的發(fā)展及生物信息學(xué)、蛋白質(zhì)組學(xué)等生物技術(shù)的成熟，基因組重排技術(shù)必然成為日后開(kāi)發(fā)新產(chǎn)品、新物種的有力技術(shù)，為加速微生物菌種改良，進(jìn)一步促進(jìn)產(chǎn)業(yè)化做出貢獻(xiàn).參考文獻(xiàn)[1]ZhangYX.,PerryK.,VinciVA.,[J].Nature,2002,415(6872):644-646.[2]PowellKA.,RamerSW.,StephenB.,etEvolutionandBiocatalysis[J].AngewandteChemieernationalEdition,2001,40(21):3948-3959.[3]StemmerWPBreedingofGenes,PathwaysandGenomesbyDnauffling[J].Biotechnology&BicessEngineering,2002,7(3):121-129.[4]李亮,林娟。天津食品微生物菌種定向改良合成途徑只有采取不同機(jī)制(高溫滅活、紫外線滅活、化學(xué)試劑滅活等)滅活的親本之間才能發(fā)生互補(bǔ)融合并恢復(fù)再生；</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇冶金微生物菌種定向改良實(shí)驗(yàn)服務(wù),微生物菌種定向改良" src="http://tyunfile.71360.com/UploadFile/shchaorui/637190230318701172/9190410.png"></div><p> 兩者值越高說(shuō)明蛋白質(zhì)質(zhì)量越好。核酸是所有細(xì)胞的必需成分，并且在快速分裂的細(xì)胞中呈現(xiàn)出相對(duì)高的水平，如果攝入過(guò)多RNA就會(huì)導(dǎo)致人體尿酸含量高于安全標(biāo)準(zhǔn)，所以去除SCP產(chǎn)品中的核酸是關(guān)乎安全性的重要問(wèn)題[11]。5、單細(xì)胞蛋白在食品工業(yè)的應(yīng)用前景展望、單細(xì)胞蛋白的應(yīng)用前景(1)SCP含有豐富的蛋白質(zhì)及多種維生素和無(wú)機(jī)鹽，是一種營(yíng)養(yǎng)較為***的理想蛋白質(zhì)來(lái)源，把它摻和在餅干、飲料、奶制品中，能提高這些傳統(tǒng)食品的營(yíng)養(yǎng)價(jià)值;(2)SCP除直接作為食品應(yīng)用外，還可作為一種食品蛋白質(zhì)組分添加到湯料或飲料中強(qiáng)化營(yíng)養(yǎng)、增加食品的強(qiáng)度;(3)利用單細(xì)胞蛋白質(zhì)的蛋白質(zhì)**形成性可用于制造“人造肉”等新食品;(4)SCP還常作為食品添加劑，用以補(bǔ)充蛋白質(zhì)或維生素、礦物質(zhì)等;(5)SCP運(yùn)用于肉制品和焙烤食品的制作中，可保持食品水分、口感和風(fēng)味等;(6)SCP能提高食品的某些物理性能，如意大利烘餅中加入活性酵母可提高餅的延薄性能，酵母濃縮鮮劑蛋白具有顯著的鮮味而***用作食品的增鮮劑。、單細(xì)胞蛋白的生產(chǎn)現(xiàn)狀及前景展望我國(guó)SCP生產(chǎn)始于1922年，1986年4月10日，我國(guó)***個(gè)SCP飼料廠在廣東省江門市建成并試產(chǎn)成功。1987年全國(guó)SCP(酵母)總產(chǎn)量，其中固體酵母達(dá)。</p><p style="text-indent:25px">微生物菌劑無(wú)論是用作土壤修復(fù)菌劑或者是微生物菌肥，都有一個(gè)不可回避的問(wèn)題，即微生物菌種在土壤環(huán)境中的競(jìng)爭(zhēng)存活能力。競(jìng)爭(zhēng)力強(qiáng)的菌種能夠很快適應(yīng)所處的土壤環(huán)境，迅速繁殖成為優(yōu)勢(shì)菌群，從而發(fā)揮其***土壤有機(jī)質(zhì)、抵御有害菌群、刺激植物生長(zhǎng)的作用，同時(shí)還可以達(dá)到修復(fù)土壤、培肥地力、防治土傳病害、提高作物產(chǎn)量和品質(zhì)之目的。競(jìng)爭(zhēng)力差的菌種，不僅作用大打折扣，而且在土壤菌群數(shù)量會(huì)迅速衰減，存活都較為困難。。。。。。基因組重排突變菌的***篩選和驗(yàn)證。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇冶金微生物菌種定向改良實(shí)驗(yàn)服務(wù),微生物菌種定向改良" src="http://tyunfile.71360.com/UploadFile/shchaorui/637190230319501953/3670731.png"></div><p> 原標(biāo)題：【觀點(diǎn)】微生物菌劑中的“野老虎”和“寵物貓”微生物菌劑無(wú)論是用作土壤修復(fù)菌劑或者是微生物菌肥，都有一個(gè)不可回避的問(wèn)題，即微生物菌種在土壤環(huán)境中的競(jìng)爭(zhēng)存活能力。競(jìng)爭(zhēng)力強(qiáng)的菌種能夠很快適應(yīng)所處的土壤環(huán)境，迅速繁殖成為優(yōu)勢(shì)菌群，從而發(fā)揮其***土壤有機(jī)質(zhì)、抵御有害菌群、刺激植物生長(zhǎng)的作用，同時(shí)還可以達(dá)到修復(fù)土壤、培肥地力、防治土傳病害、提高作物產(chǎn)量和品質(zhì)之目的。競(jìng)爭(zhēng)力差的菌種，不僅作用大打折扣，而且在土壤菌群數(shù)量會(huì)迅速衰減，存活都較為困難。鑒于國(guó)家相關(guān)政策的傾斜，一些不具備生產(chǎn)微生物菌劑的企業(yè)也跟風(fēng)上馬，加入到土壤修復(fù)菌劑和微生物菌肥的生產(chǎn)行列。企業(yè)沒(méi)有具有自主知識(shí)產(chǎn)權(quán)的菌種，大都采用外購(gòu)菌種的形式解決生產(chǎn)問(wèn)題，但是不清楚由于微生物個(gè)體很小，與外界環(huán)境的接觸面很大，在人工重復(fù)培養(yǎng)繁殖過(guò)程中極易發(fā)生變異，喪失其原來(lái)的一些特性，特別是野生環(huán)境下的競(jìng)爭(zhēng)存活能力。這樣生產(chǎn)出來(lái)的菌劑產(chǎn)品好比自家養(yǎng)的寵物貓，進(jìn)入自然的生態(tài)環(huán)境后往往自身難保，見(jiàn)了耗子都會(huì)嚇怕的情況下，怎么能抓住耗子。喪失野生環(huán)境下競(jìng)爭(zhēng)存活能力的菌劑，也是如此，根本無(wú)法發(fā)揮其抵御有害菌群、***土壤有機(jī)質(zhì)等作用。這種實(shí)用***的技術(shù)不僅是傳統(tǒng)育種方法的有效補(bǔ)充和延伸；北京工業(yè)微生物菌種定向改良耐受性</p><p style="text-indent:25px">可以突破微生物種屬間的限制；江蘇冶金微生物菌種定向改良實(shí)驗(yàn)服務(wù)</p><p> 動(dòng)物園里圈在籠子的老虎現(xiàn)在都叫做“大貓”，已經(jīng)完全喪失野外生存的本領(lǐng)，把它放出去不是被別人吃掉，而是自己把自己活活餓死。我們每次看到動(dòng)物園放歸野生動(dòng)物，都要讓它適應(yīng)很長(zhǎng)時(shí)間的野外生產(chǎn)環(huán)境才敢放它歸林，這就是為了恢復(fù)它的野性，北京八達(dá)嶺野生動(dòng)物園的老虎就比較具有野性。掌握了野性十足的菌種，還必須有與之配套能夠保持菌種野性的發(fā)酵培育方法、防止菌種變異辦法、菌種提純辦法、防止交叉污染辦法和菌種復(fù)壯保存辦法，可見(jiàn)菌劑的生產(chǎn)工藝也至關(guān)重要。良好的生產(chǎn)工藝不僅要比較大限度的保證活菌接種體的數(shù)量和活力，還要盡可能保留菌種生長(zhǎng)繁殖過(guò)程中分泌的次生代謝產(chǎn)物，這兩者的結(jié)合體才能保證菌種在自然界立于不敗之地，才是完整且具野性的合格菌劑。大凡了解微生物特性的企業(yè)負(fù)責(zé)人和技術(shù)人員，都很注重原始菌種的保藏和復(fù)壯，同時(shí)會(huì)花大力氣創(chuàng)新菌劑生產(chǎn)工藝，持續(xù)保存菌劑產(chǎn)品的野老虎特性。我們?cè)陂L(zhǎng)期的研究和生產(chǎn)實(shí)踐中，不僅要對(duì)具有自主知識(shí)產(chǎn)權(quán)的原菌種定期復(fù)壯，還要不斷創(chuàng)新菌劑生產(chǎn)工藝，保存恢復(fù)菌種的野老虎特性，同時(shí)還要根據(jù)不同地域和環(huán)境的差異，定向改良原菌種。江蘇冶金微生物菌種定向改良實(shí)驗(yàn)服務(wù)</p><p style="text-indent:25px">上海朝瑞生物科技有限公司是一家1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長(zhǎng) 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗(yàn)檢測(cè)服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級(jí)改造產(chǎn)品服務(wù) 9.發(fā)布實(shí)驗(yàn)室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項(xiàng)目團(tuán)隊(duì)招聘13.發(fā)布研究生婚戀 14.發(fā)布項(xiàng)目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù)的公司，致力于發(fā)展為創(chuàng)新務(wù)實(shí)、誠(chéng)實(shí)可信的企業(yè)。上海朝瑞科技深耕行業(yè)多年，始終以客戶的需求為向?qū)В瑸榭蛻籼峁?**的科研技術(shù)服務(wù)，應(yīng)用技術(shù)服務(wù)，科研成果轉(zhuǎn)化，科學(xué)產(chǎn)品銷售。上海朝瑞科技始終以本分踏實(shí)的精神和必勝的信念，影響并帶動(dòng)團(tuán)隊(duì)取得成功。上海朝瑞科技創(chuàng)始人仲緒軍，始終關(guān)注客戶，創(chuàng)新科技，竭誠(chéng)為客戶提供良好的服務(wù)。</p></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/1349659.html</p></div></div></div></div></div><div id="gfbsyvc" class="page-right twoColRt"><div id="7fqhdua" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="8rnjuaw" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img 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