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href="http://www.cdcfah.com/zh/"><span>展會(huì)信息</span></a></li></ul></div></nav><section class="wrap clearfix"><section class="container clearfix tcPro"><section class="home-main container clearfix"><div id="lrxlj75" class="twoCol"><div id="lrxvtr9" class="page-proshow clearfix"><p class="current"> 當(dāng)前位置:<a href="http://www.cdcfah.com/">首頁(yè)?<a href="http://www.cdcfah.com/cp/">產(chǎn)品供應(yīng)</a>?<a href="http://www.cdcfah.com/cp/second-_nongye/">農(nóng)業(yè)</a>?<a href="http://www.cdcfah.com/cp/third-nongye_nlmyxmhz/">農(nóng)林牧漁項(xiàng)目合作</a>?上海微量蛋白Western Blot技術(shù)服務(wù) 歡迎來(lái)電上海朝瑞生物科技供應(yīng)</p><div id="fbz9jlr" class="leftbox clearfix"><h1>上海微量蛋白Western Blot技術(shù)服務(wù) 歡迎來(lái)電上海朝瑞生物科技供應(yīng)</h1><div id="fvbzhnl" class="proBig"><div id="h7zpft7" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637115400031269532/7671383.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="jxvlrnb" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637115400031269532/7671383.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="jhflbhf" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>上海微量蛋白Western Blot技術(shù)服務(wù)</p><p><span>***更新：</span>2020-06-05 12:13:46</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="ptbz9v7" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="vt79vlb" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="pfndb9l" class="box"><p><p> 收集蛋白樣品(Protein sample preparation) </p> <p> 可以使用適當(dāng)?shù)牧呀庖?，Western及IP細(xì)胞裂解液、RIPA裂解液等，裂解貼壁細(xì)胞、懸浮細(xì)胞或組織樣品。對(duì)于某些特定的亞細(xì)胞組份蛋白，例如細(xì)胞**白、細(xì)胞漿蛋白、線粒體蛋白等，上海微量蛋白Western Blot技術(shù)服務(wù)，可以參考相關(guān)文獻(xiàn)提取這些亞細(xì)胞組份蛋白，也可以使用試劑盒進(jìn)行抽提。收集完蛋白樣品后，為確保每個(gè)蛋白樣品的上樣量一致，需要測(cè)定每個(gè)蛋白樣品的蛋白濃度，上海微量蛋白Western Blot技術(shù)服務(wù)。根據(jù)所使用的裂解液的不同，需要采用適當(dāng)?shù)牡鞍诐舛葴y(cè)定方法，上海微量蛋白Western Blot技術(shù)服務(wù)。因?yàn)椴煌牡鞍诐舛葴y(cè)定方法對(duì)于一些去垢劑和還原劑等的兼容性差別很大。如果使用Western及IP細(xì)胞裂解液或RIPA裂解液，可以使用BCA蛋白濃度測(cè)定試劑盒 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海微量蛋白Western Blot技術(shù)服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637115400031269532/7671383.png"></div><p> 免疫印跡法（immunoblotting ）因與Southern早先建立的檢測(cè)核酸的印跡方法Southern </p> <p> blot相類似，亦被稱為Western blot。免疫印跡（western blotting）是在蛋白質(zhì)電泳分離和抗原抗體檢測(cè)的基礎(chǔ)上發(fā)展起來(lái)一項(xiàng)檢測(cè)蛋白質(zhì)的技術(shù)。它將SDS聚丙烯酰胺凝膠電泳的辨力與抗原抗體反應(yīng)的特異性相結(jié)合。典型的印跡實(shí)驗(yàn)包括三個(gè)步驟①蛋白質(zhì)的電泳分離；②將電泳后凝膠上的蛋白質(zhì)轉(zhuǎn)移至固體膜上，用非特異性，非反應(yīng)活性分子封閉固體膜上未吸附蛋白質(zhì)區(qū)域；③免疫學(xué)檢測(cè)。免疫印跡克服了聚丙烯酰胺凝膠電泳后直接在凝膠上進(jìn)行免疫學(xué)分析的弊端，極大地提高了其利用率、分辨率和靈敏度，使其成為使用**的免疫化學(xué)方法之一。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海微量蛋白Western Blot技術(shù)服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637115400026982422/5756533.png"></div><p> (1) SDS-PAGE凝膠配制 </p> <p> SDS-PAGE凝膠可以使用商業(yè)生產(chǎn)的SDS-PAGE凝膠配制試劑盒。 </p> <p> (2) 樣品處理：在收集的蛋白樣品中加入適量濃縮的SDS-PAGE蛋白上樣緩沖液。例如2X或5X的SDS-PAGE蛋白上樣緩沖液。使用5X的SDS-PAGE蛋白上樣緩沖液可以減小上樣體積，在相同體積的上樣孔內(nèi)可以上樣更多的蛋白樣品。5X的SDS-PAGE蛋白上樣緩沖液可以參考相關(guān)文獻(xiàn)資料配制100℃或沸水浴加熱3-5分鐘，以充分變性蛋白。 </p> <p> (3) 上樣與電泳：冷卻到室溫后，把蛋白樣品直接上樣到SDS-PAGE膠加樣孔內(nèi)即可。為了便于觀察電泳效果和轉(zhuǎn)膜效果，以及判斷蛋白分子量大小，比較好使用預(yù)染蛋白質(zhì)分子量標(biāo)準(zhǔn)(P0066)。電泳時(shí)通常推薦在上層膠時(shí)使用低電壓恒壓電泳，而在溴酚藍(lán)進(jìn)入下層膠時(shí)使用高電壓恒壓電泳。對(duì)于Bio-Rad的標(biāo)準(zhǔn)電泳裝置或類似電泳裝置，低電壓可以設(shè)置在80-100V，高電壓可以設(shè)置在120V左右。SDS-PAGE可以采用普通的電泳儀就可以滿足要求。通常電泳時(shí)溴酚藍(lán)到達(dá)膠的底端處附近即可停止電泳，或者可以根據(jù)預(yù)染蛋白質(zhì)分子量標(biāo)準(zhǔn)的電泳情況，預(yù)計(jì)目的蛋白已經(jīng)被適當(dāng)分離后即可停止電泳。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海微量蛋白Western Blot技術(shù)服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637115400031748047/9643065.png"></div></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/157156.html</p></div></div></div></div></div><div id="xn9v7rh" class="page-right twoColRt"><div 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