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src="http://tyunfile.71360.com/UploadFile/shchaorui/637115400028759766/3475292.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="pcijuaz" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637115400028759766/3475292.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="um3kvqa" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>上海長(zhǎng)條帶Western Blot實(shí)驗(yàn)外包</p><p><span>***更新：</span>2020-06-06 00:24:32</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="3wm3dfz" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="spv9a8m" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="7ee9pcc" class="box"><p><p> 收集蛋白樣品(Protein sample preparation) </p> <p> 可以使用適當(dāng)?shù)牧呀庖海琖estern及IP細(xì)胞裂解液、RIPA裂解液等，裂解貼壁細(xì)胞、懸浮細(xì)胞或組織樣品。對(duì)于某些特定的亞細(xì)胞組份蛋白，例如細(xì)胞**白，上海長(zhǎng)條帶Western Blot實(shí)驗(yàn)外包、細(xì)胞漿蛋白、線粒體蛋白等，可以參考相關(guān)文獻(xiàn)提取這些亞細(xì)胞組份蛋白，也可以使用試劑盒進(jìn)行抽提，上海長(zhǎng)條帶Western Blot實(shí)驗(yàn)外包。收集完蛋白樣品后，為確保每個(gè)蛋白樣品的上樣量一致，需要測(cè)定每個(gè)蛋白樣品的蛋白濃度。根據(jù)所使用的裂解液的不同，上海長(zhǎng)條帶Western Blot實(shí)驗(yàn)外包，需要采用適當(dāng)?shù)牡鞍诐舛葴y(cè)定方法。因?yàn)椴煌牡鞍诐舛葴y(cè)定方法對(duì)于一些去垢劑和還原劑等的兼容性差別很大。如果使用Western及IP細(xì)胞裂解液或RIPA裂解液，可以使用BCA蛋白濃度測(cè)定試劑盒 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海長(zhǎng)條帶Western Blot實(shí)驗(yàn)外包" src="http://tyunfile.71360.com/UploadFile/shchaorui/637115400028759766/3475292.png"></div><p> 1.為SDS—聚丙烯酰胺凝膠電泳（SDS-PAGE）?？乖鹊鞍讟悠方?jīng)SDS處理后帶陰電荷，在聚丙烯酰膪凝膠中從陰極向陽(yáng)極泳動(dòng)，分子量越小，泳動(dòng)速度就越快。此階段分離效果肉眼不可見(jiàn)（只有在染色后才顯出電泳區(qū)帶）。2.電轉(zhuǎn)移。將在凝膠中已經(jīng)分離的條帶轉(zhuǎn)移至硝酸纖維素膜上，選用低電壓（100V）和大電流（1～2A），通電45min轉(zhuǎn)移即可完成。此階段分離的蛋白質(zhì)條帶肉眼仍不可見(jiàn)。3.酶免疫定位。將印有蛋白質(zhì)條帶的硝酸纖維素膜（相當(dāng)于包被了抗原的固相載體）依次與特異性抗體和酶標(biāo)第二抗體作用后，加入能形成不溶性顯色物的酶反應(yīng)底物，使區(qū)帶染色。常用的HRP底物為3，3，—二氨基聯(lián)苯胺（呈棕色）和4-氯-1-萘酚（呈藍(lán)紫色）。陽(yáng)性反應(yīng)的條帶清晰可辨，并可根據(jù)SDS-PAGE時(shí)加人的分子量標(biāo)準(zhǔn)，確定各組分的分子量。本法綜合了SDS-PAGE的辨力和ELISA法的高特異性和敏感性，是一個(gè)有效的分析手段，不僅廣泛應(yīng)用于分析抗原組分及其免疫活性，并可用于疾病的診斷。在**中此法作為確診試驗(yàn)。抗原經(jīng)電泳轉(zhuǎn)移在硝酸纖維素膜上后，將膜切成小條，配合酶標(biāo)抗體及顯色底物制成的試劑盒可方便地在實(shí)驗(yàn)室中供檢測(cè)用。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海長(zhǎng)條帶Western Blot實(shí)驗(yàn)外包" src="http://tyunfile.71360.com/UploadFile/shchaorui/637115400026757813/4194644.png"></div><p style="text-indent:25px">全自動(dòng)單細(xì)胞western blot檢測(cè)分析原理：全自動(dòng)單細(xì)胞western blot分析是單細(xì)胞水平，蛋白質(zhì)表達(dá)定量分析系統(tǒng)。系統(tǒng)采用**的微流控western blot芯片，通過(guò)單細(xì)胞微孔設(shè)計(jì)，采集單細(xì)胞，然后原位裂解細(xì)胞，釋放蛋白，進(jìn)行蛋白質(zhì)電泳，將不同分子量蛋白進(jìn)行分離，提高免疫學(xué)檢測(cè)特異性。之后，采用**技術(shù)進(jìn)行蛋白質(zhì)原位捕獲，使用western blot驗(yàn)證抗體及熒光標(biāo)記二抗直接雜交，掃描儀進(jìn)行芯片掃描后，分析軟件對(duì)掃描結(jié)果進(jìn)行深度定量分析。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海長(zhǎng)條帶Western Blot實(shí)驗(yàn)外包" src="http://tyunfile.71360.com/UploadFile/shchaorui/637115400028710938/8932273.png"></div></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/158993.html</p></div></div></div></div></div><div id="fcsgzfe" class="page-right twoColRt"><div id="8r8yujt" class="box01 margin10 clearfix"><h3><a 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