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style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>上海動物細(xì)胞永生化</p><p><span>***更新：</span>2020-06-06 07:12:34</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="rnldvbn" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="t9x7ht7" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="lrjp9pd" class="box"><p><p> —個性化定制您理想的細(xì)胞系細(xì)胞永生化（Cellimmortalization）是指體外培養(yǎng)的細(xì)胞經(jīng)過自發(fā)的或受外界因素的影響從增殖衰老危機(jī)中逃離，從而具有無限增殖能力的過程。正常組織來源的細(xì)胞在通常的體外培養(yǎng)條件下可分裂生長，但經(jīng)過有限次數(shù)的傳代后，就會停止增殖，發(fā)生衰老和死亡。而細(xì)胞培養(yǎng)是一項基本的科研技術(shù)，無論是基礎(chǔ)的科學(xué)研究，還是在臨床***，上海動物細(xì)胞永生化，以及生物工程制藥，疫苗研發(fā)等方面，都發(fā)揮著重要的作用。利用基因轉(zhuǎn)染等技術(shù)將外源性永生化基因?qū)肽康募?xì)胞內(nèi)，從而建立永生化細(xì)胞株，以達(dá)到使體外培養(yǎng)的細(xì)胞具有無限增殖能力且細(xì)胞間無差異的目的。對細(xì)胞永生化進(jìn)行深入研究，不僅有利于我們了解細(xì)胞增殖與衰老的分子機(jī)制，上海動物細(xì)胞永生化，而且為探尋*****及***移植的研究奠定了堅實的基礎(chǔ)。此外，由于永生化細(xì)胞具有可以多次傳代的特性，可以利用各種細(xì)胞永生化的方法使那些傳代困難，上海動物細(xì)胞永生化、增殖緩慢、容易衰老的細(xì)胞獲得永生，從而為研究人員提供更多的細(xì)胞資源，為科學(xué)研究提供了良好的體外細(xì)胞模型。細(xì)胞永生化的機(jī)理與建立方法由于體外培養(yǎng)細(xì)胞自發(fā)性永生化率非常低，目前普遍采用基因轉(zhuǎn)染技術(shù)將外源性永生化基因?qū)肽康募?xì)胞內(nèi),以增加永生化。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海動物細(xì)胞永生化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256597216797/1743672.png"></div><p> 一、試劑 </p> <p> 1、淋巴細(xì)胞分離液：避光4度保存，用前在37度水浴中加溫。整個分離過程中，溫度應(yīng)控制在8-28度，溫度過高或過低均影響分離質(zhì)量。 </p> <p> 2、全培養(yǎng)基（用前配置）：RPM1640培養(yǎng)基，內(nèi)含20%胎牛血清（Gibco）{56℃滅活30分鐘}，1.6-1.8% 1M的HEPES，1.2% 0.2mol/L谷氨酰胺，1%青霉素和鏈霉素。 </p> <p> 3、環(huán)胞酶素A（CyA）：5ml(250mg)/瓶，用1640培養(yǎng)基稀釋成0.2mg/ml，使用時終濃度為2ug/ml(0.5%)。 </p> <p> 4、 EBV液購自中國科學(xué)院遺傳所，儲存于零下80度。使用時從冰箱中取出，37度迅速融化，用0.22um的濾膜過濾，不要超過0.5-1小時。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海動物細(xì)胞永生化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256594443359/8073132.png"></div><p> 二、建株方法 </p> <p> 1、取外周血3-4ml，肝素抗凝（血液室溫可放置48小時），應(yīng)用液濃度5u/ml，可加入5-10ml外周血。 </p> <p> 2、將血液與等量1640培養(yǎng)液混勻后，沿管壁漸漸加入到含有4ml淋巴細(xì)胞分離液的離心管中（混合血：淋巴細(xì)胞分離液=6：4），3000轉(zhuǎn)離心10分鐘。 </p> <p> 3、吸取白細(xì)胞層，移入另一支離心管中，加入不含血清的1640培養(yǎng)液6ml，輕輕混勻，進(jìn)行***次洗滌。1500轉(zhuǎn)離心15分鐘。 </p> <p> 4、棄上清液，再加6ml1640全培養(yǎng)液進(jìn)行第二次洗滌，離心1500轉(zhuǎn)15分鐘。 </p> <p> 5、棄上清液，加入2ml1640全培養(yǎng)基（全培養(yǎng)基加入前，置37度水浴10分鐘），然后加入環(huán)胞霉素A（4%）和1.2mlEBV液/份，充分混勻后移入到25平方厘米的培養(yǎng)瓶，置37度，5%CO2培養(yǎng)箱中。 </p> <p> 6、每2-3天加入3ml新鮮配制的培養(yǎng)基（1.6% 1M HEPES 緩沖液；15%胎牛血清（FBS）；1%青霉素和鏈霉素；PRMI 1640補(bǔ)足到**）。7天左右鏡下觀察，可見淋巴細(xì)胞明顯增大，出現(xiàn)聚集現(xiàn)象。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海動物細(xì)胞永生化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637122257125664062/2868592.png"></div></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/164571.html</p></div></div></div></div></div><div id="xpxxp9j" class="page-right twoColRt"><div id="95jrxff" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="lvvhdt9" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" alt="福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)" class="vcenter" 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