浙江專業(yè)蛋白純化系統(tǒng)制造廠家蘇州英賽斯智能科技供應(yīng)-b2b網(wǎng)站_b2b電子商務(wù)平臺(tái)【買產(chǎn)品】-上?？谄蚴称酚邢薰?/title><meta name="description" content="蛋白質(zhì)分離純化步驟（二）2.4樣品的進(jìn)一步分離純化用等電點(diǎn)沉淀法、鹽析法所得到的蛋白質(zhì)一般含有其他蛋白質(zhì)雜質(zhì)，須進(jìn)一步分離提純才能得到有一定純度的樣品。常用的純化方法有：凝膠過濾層析、離子交換纖維素層析、親和層析等等。有時(shí)還需要這幾種方法聯(lián)"><meta name="keywords" content="浙江專業(yè)蛋白純化系統(tǒng)制造廠家,蛋白純化系統(tǒng)"><link rel="canonical" href="http://www.cdcfah.com/"/><meta name="applicable-device" content="pc"><link rel="shortcut icon" href="/static/new_net_01/images/favicon.ico"><link rel="stylesheet" href="/static/new_net_01/css/slick.css" /><link rel="stylesheet" href="/static/new_net_01/css/addnew.css"><link rel="stylesheet" href="/static/new_net_01/css/sidenav.css"><link rel="stylesheet" href="/static/new_net_01/css/style.css" /><link rel="stylesheet" href="/static/new_net_01/css/tab.css"><link rel="stylesheet" href="/static/new_net_01/css/404.css"> <meta name="baidu-site-verification" content="OBVzrC9aaQ" /> <meta 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id="omtirky" class="twoCol"><div id="2yfokzv" class="page-proshow clearfix"><p class="current"> 當(dāng)前位置:<a href="http://www.cdcfah.com/">首頁(yè)?<a href="http://www.cdcfah.com/cp/">產(chǎn)品供應(yīng)</a>?<a href="http://www.cdcfah.com/cp/first-_yiqiyibiao/">儀器儀表</a>?<a href="http://www.cdcfah.com/cp/second-yiqiyibiao_swyqxm/">生物儀器</a>?<a href="http://www.cdcfah.com/cp/third-swyqxm_qtswyq/">其他生物儀器</a>?浙江專業(yè)蛋白純化系統(tǒng)制造廠家蘇州英賽斯智能科技供應(yīng)</p><div id="knp33o7" class="leftbox clearfix"><h1>浙江專業(yè)蛋白純化系統(tǒng)制造廠家蘇州英賽斯智能科技供應(yīng)</h1><div id="w22hho7" class="proBig"><div id="g3cqhta" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UpLoadFile/szyss/2019/8/637020707435096926/6370207074350969265706238.jpg" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="o3ikri8" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UpLoadFile/szyss/2019/8/637020707435096926/6370207074350969265706238.jpg" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="eipjfm7" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>江蘇省</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>浙江專業(yè)蛋白純化系統(tǒng)制造廠家,蛋白純化系統(tǒng)</p><p><span>***更新：</span>2020-11-11 21:12:30</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="x7hjamq" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">蘇州英賽斯智能科技有限公司</a></p><p><span>聯(lián)系人：</span>李大為</p><p><span>郵箱: </span>570745634@qq.com</p><p><span>電話: </span>18606243033</p><p><span>傳真: </span>0512_63937379</p><p><span>網(wǎng)址: </span>http://www.inscinstech.com.cn</p><p><span>手機(jī): </span>0512-63937378</p><p><span>地址: </span>吳江經(jīng)濟(jì)技術(shù)開發(fā)區(qū)聯(lián)楊路南側(cè)、龍橋路西側(cè)</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="oeacoac" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="hpwd8ys" class="box"><p><p style="text-indent:25px">蛋白質(zhì)分離純化步驟（二） 2.4 樣品的進(jìn)一步分離純化用等電點(diǎn)沉淀法、鹽析法所得到的蛋白質(zhì)一般含有其他蛋白質(zhì)雜質(zhì)，須進(jìn)一步分離提純才能得到有一定純度的樣品。常用的純化方法有：凝膠過濾層析、離子交換纖維素層析、親和層析等等。有時(shí)還需要這幾種方法聯(lián)合使用才能得到較高純度的蛋白質(zhì)樣品。 1. 凝膠過濾層析凝膠過濾層析（gel-filtration chromatography）又稱為分子排阻層析或分子篩層析。它是將葡聚糖凝膠（Sephadex）裝入一個(gè)柱子中，制成凝膠柱。這種凝膠顆粒具有網(wǎng)狀結(jié)構(gòu)，不同類型凝膠的網(wǎng)孔大小不同。當(dāng)把蛋白質(zhì)混合樣品加到凝膠柱中時(shí)，比凝膠網(wǎng)孔小的蛋白質(zhì)可進(jìn)入網(wǎng)孔內(nèi)，而大于網(wǎng)孔的分子則不能進(jìn)入被排阻在凝膠顆粒之外。當(dāng)用洗脫液洗脫時(shí)，浙江專業(yè)蛋白純化系統(tǒng)制造廠家，被排阻的分子量大的蛋白質(zhì)直接通過凝膠之間的縫隙先被洗脫下來(lái)，而比網(wǎng)孔小的蛋白質(zhì)可連續(xù)不斷地進(jìn)入網(wǎng)孔內(nèi)。這樣的小分子不但流經(jīng)的路程長(zhǎng)，而且受到來(lái)自凝膠內(nèi)部的阻力也很大，浙江專業(yè)蛋白純化系統(tǒng)制造廠家，所以蛋白質(zhì)越小，浙江專業(yè)蛋白純化系統(tǒng)制造廠家，從柱子上洗脫下來(lái)所需時(shí)間越長(zhǎng)。由于不同蛋白質(zhì)的分子大小不同，進(jìn)入網(wǎng)孔的程度不同，因此流出的速度不同，洗脫所用體積及時(shí)間不同，從而達(dá)到分離的目的。浙江專業(yè)蛋白純化系統(tǒng)制造廠家</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="浙江專業(yè)蛋白純化系統(tǒng)制造廠家,蛋白純化系統(tǒng)" src="http://tyunfile.71360.com/UpLoadFile/szyss/2019/8/637020707435096926/6370207074350969265706238.jpg"></div><p style="text-indent:25px">實(shí)驗(yàn)室及中試放大規(guī)模蛋白純化系統(tǒng) Unique Autopure系列蛋白純化系統(tǒng) 主要應(yīng)用領(lǐng)域： – 蛋白純化系統(tǒng)主要應(yīng)用于單抗、重組蛋白、疫苗、生化藥、、天然產(chǎn)物和多糖等生物制藥領(lǐng)域。用于生物制品的下游工藝的分離純化。 Unique AutoPure 系列蛋白純化系統(tǒng) ：產(chǎn)品特點(diǎn)及優(yōu)勢(shì)： – 模塊化設(shè)計(jì)，提供多個(gè)配置，滿足特殊工藝流程需求 - 低脈動(dòng)高精度雙柱塞輸液泵，保證優(yōu)異的分離重現(xiàn)性 ; – 全系統(tǒng)與液體接觸的地方均由生物兼容性材料組成、符合FDA、USP VI等相關(guān)法規(guī)要求 ; – DAD全譜直讀檢測(cè)器，避免了傳統(tǒng)的機(jī)械切換式檢測(cè)器所帶來(lái)的不穩(wěn)定性及高故障率 ; – 固定波長(zhǎng)檢測(cè)器燈為長(zhǎng)壽命LED燈，壽命大于8000小時(shí)，降低維護(hù)成本 ; – **技術(shù)紫外檢測(cè)器流通池，低死體積、低峰拓展，提高了色譜分離度 ; – 高靈敏度在線pH、電導(dǎo)率、紫外檢測(cè)器，低死體積、低峰拓展，提高了色譜分離度 ; – 集中了buffer選擇閥，自動(dòng)進(jìn)樣閥、柱位閥等自動(dòng)化組件，提高了純化的自動(dòng)化程度 ; – 具有柱前、柱后、壓差報(bào)警，氣泡傳感器檢測(cè)等功能，極大的保護(hù)了系統(tǒng)及層析柱的安全性 ; – 層析工作站軟件是一款功能強(qiáng)大、性能先進(jìn)、高穩(wěn)定性的色譜數(shù)據(jù)管理系浙江專業(yè)蛋白純化系統(tǒng)制造廠家</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="浙江專業(yè)蛋白純化系統(tǒng)制造廠家,蛋白純化系統(tǒng)" src="http://tyunfile.71360.com/UpLoadFile/szyss/2020/1/637138171561815352/6371381715618153525073938.jpg"></div><p style="text-indent:25px">蛋白質(zhì)分離純化步驟（二） 2.4.2 纖維素柱層析法該法是利用蛋白質(zhì)的酸堿性質(zhì)作為分離的基礎(chǔ)。離子交換纖維素（cellulose ion-exchanger）是人工合成的纖維素衍生物，它具有松散的親水性網(wǎng)狀結(jié)構(gòu)，有較大的表面積，使蛋白質(zhì)大分子可以自由通過。因此常用于蛋白質(zhì)的分離。（1）羧甲基纖維素（CM-纖維素）在纖維素顆粒上帶有羧甲基基團(tuán)。在中性pH條件下，羧甲基上的質(zhì)子可解離下來(lái)（圖2-27a），而溶液中帶正電荷的蛋白質(zhì)分子可與纖維素顆粒上的羧甲基負(fù)電荷結(jié)合。可交換的基團(tuán)帶正電，因此是一種陽(yáng)離子交換劑。蛋白質(zhì)與離子交換纖維素之間結(jié)合能力的大小取決于彼此間相反電荷基團(tuán)之間靜電吸引。 (2）二乙氨基乙基纖維素（DEAE-纖維素）在中性pH條件下，它含有帶正電荷的基團(tuán)，可與溶液中的帶負(fù)電荷的蛋白質(zhì)結(jié)合，可交換的基團(tuán)帶負(fù)電荷，因此是一種陰離子交換劑。當(dāng)某一蛋白質(zhì)混合溶液通過裝有DEAE-纖維素的層析柱時(shí)，帶正電荷的蛋白質(zhì)不能結(jié)合而隨著洗脫液的流動(dòng)先被洗脫下來(lái)。帶負(fù)電荷的蛋白質(zhì)將被結(jié)合到柱上。結(jié)合力取決于彼此相反電荷基團(tuán)間的靜電吸引。然后選用一定pH和離子強(qiáng)度的緩沖液進(jìn)行洗脫，改變蛋白質(zhì)分子所帶的靜電荷，依次從層析柱流出達(dá)到相互分離的目的。</p><p style="text-indent:25px">三、蛋白質(zhì)分子量的測(cè)定 3.2 SDS-聚丙烯酰胺凝膠電泳法測(cè)定分子量蛋白質(zhì)在普通聚丙烯酰胺凝膠中的電泳速度取決于蛋白質(zhì)分子大小、所帶電荷的量以及分子形狀。而SDS-聚丙烯酰胺凝膠電泳與此不同的是在樣品及電泳緩沖液中加入了十二烷基***鈉（sodium dodecylsulfate,SDS）。SDS是一種陰離子去污劑，可使蛋白質(zhì)變性并解離成亞基。當(dāng)?shù)鞍踪|(zhì)樣品中加入SDS（一般加入量為0.1％）后，SDS與蛋白質(zhì)分子結(jié)合，使蛋白質(zhì)分子帶上大量的負(fù)電荷，這些電荷量遠(yuǎn)遠(yuǎn)超過蛋白質(zhì)分子原來(lái)所帶的電荷量，因而掩蓋了不同蛋白質(zhì)之間的電荷差異。所有結(jié)合SDS的蛋白質(zhì)-SDS復(fù)合物的形狀近似于長(zhǎng)的橢園棒，它們的短軸是恒定的，而長(zhǎng)軸與蛋白質(zhì)分子量的大小成正比。這樣，消除了蛋白質(zhì)之間原有的電荷和形狀的差異，電泳的速度只取決于蛋白質(zhì)分子量的大小。進(jìn)行凝膠電泳時(shí)，常常用一種染料作前沿物質(zhì)，蛋白質(zhì)分子在電泳中的移動(dòng)距離和前沿物質(zhì)移動(dòng)的距離之比值稱為相對(duì)遷移率，相對(duì)遷移率和分子質(zhì)量的對(duì)數(shù)成直線關(guān)系。以標(biāo)準(zhǔn)蛋白質(zhì)分子質(zhì)量的對(duì)數(shù)和其相對(duì)遷移率作圖，得到標(biāo)準(zhǔn)曲線。將未知蛋白質(zhì)在同樣條件下電泳，根據(jù)測(cè)得的樣品相對(duì)遷移率，從標(biāo)準(zhǔn)曲線上便可查出其分子量。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="浙江專業(yè)蛋白純化系統(tǒng)制造廠家,蛋白純化系統(tǒng)" src="http://tyunfile.71360.com/UpLoadFile/szyss/2020/1/637138172282251484/6371381722822514847644105.jpg"></div><p> 蛋白質(zhì)純化方法-親和層析親和層析法包括特異性配體親和層析法以及通用性配體親和層析法。比較這兩種親和層析法。特異性配體親和層析法通常將復(fù)雜的生命大分子物質(zhì)作為配體，它的結(jié)合力比較大河吸附選擇性比較強(qiáng)。而通用性配體親和層析法通常將簡(jiǎn)單的小分子物質(zhì)作為配體，因此，它具有低廉的成本，吸附容量比較高，通過對(duì)吸附和脫附條件的改善能夠使得層析的分辨率提高。原理親和層析的原理類似于酶-底物，***-受體與抗原-抗體等特異性反應(yīng)的機(jī)理。有一定的親和力在每對(duì)反應(yīng)物之間。與在酶與底物的反應(yīng)中，只有特異的底物才可以結(jié)合一定的酶，使復(fù)合物產(chǎn)生相同。在親和層析中，只有特異的配體才可以和一定的生命大分子有親和力，并且使復(fù)合物產(chǎn)生。親和層析配體呈固相，而酶與底物的反應(yīng)底物呈液相是它們的不同點(diǎn)。事實(shí)上，親和層析是在含有活化基團(tuán)的基質(zhì)M上以共價(jià)鍵的方式固化具有識(shí)別能力的配體L，將固相載體制作成功而固化后的配體束縛特異物質(zhì)的能力依然保持。所以當(dāng)在小層析柱裝入固相載體，使得欲分離的樣品在該柱經(jīng)過。此時(shí)，樣品中對(duì)配體有親和力的物質(zhì)S就能夠通過結(jié)構(gòu)互補(bǔ)效應(yīng)、范德瓦爾力與靜電引力等作用在固相載體上吸附著。浙江專業(yè)蛋白純化系統(tǒng)制造廠家</p><p style="text-indent:25px">浙江專業(yè)蛋白純化系統(tǒng)制造廠家</p><p style="text-indent:25px">儀器儀表行業(yè)飛速發(fā)展一是因?yàn)槲覈?guó)的經(jīng)濟(jì)高速穩(wěn)定發(fā)展的運(yùn)行;按照過去的經(jīng)驗(yàn)，如果GDP的增長(zhǎng)在10%以上時(shí)，儀表行業(yè)的增長(zhǎng)率則在26%～30%之間。二是因?yàn)槲覈?guó)宏觀調(diào)控對(duì)儀表行業(yè)的影響有一個(gè)滯后期，儀表往往在工程的后期才交付使用，因此，因宏觀調(diào)控政策而減少的收入對(duì)儀表行業(yè)的影響不會(huì)太大。隨著網(wǎng)絡(luò)消費(fèi)的不斷遞增，而互聯(lián)網(wǎng)的的商業(yè)價(jià)值不斷被挖掘出來(lái)，呈爆發(fā)式增長(zhǎng)，傳統(tǒng)的營(yíng)銷模式將逐步被取代。其他內(nèi)資企業(yè)企業(yè)要抓住機(jī)遇，融入到互聯(lián)網(wǎng)發(fā)展的行業(yè)中，為行業(yè)的發(fā)展提高競(jìng)爭(zhēng)力。蛋白純化色譜系統(tǒng)，凝膠凈化色譜系統(tǒng)，制備液相色譜，柱塞泵產(chǎn)業(yè)是國(guó)民經(jīng)濟(jì)的基礎(chǔ)性、戰(zhàn)略性產(chǎn)業(yè)，是信息化和工業(yè)化深度融合的源頭，對(duì)促進(jìn)工業(yè)轉(zhuǎn)型升級(jí)、發(fā)展戰(zhàn)略性新興產(chǎn)業(yè)、推動(dòng)現(xiàn)代**建設(shè)、保證和提高大家生活水平具有重要作用。我們必須承認(rèn)，在科學(xué)儀器上，我們跟其他地區(qū)相比，還有很大的差距。這個(gè)差距，就是我們提升的空間。合相關(guān)部門、大學(xué)和企業(yè)之力，中國(guó)的生產(chǎn)型必將在不遠(yuǎn)的將來(lái)，在相關(guān)領(lǐng)域的基礎(chǔ)研究和重點(diǎn)光學(xué)部件研發(fā)上取得突破，產(chǎn)品進(jìn)入世界中**水平，企業(yè)得到臺(tái)階式上升，迎頭趕上，與全球出名企業(yè)并駕齊驅(qū)。浙江專業(yè)蛋白純化系統(tǒng)制造廠家</p><p style="text-indent:25px">蘇州英賽斯智能科技有限公司主要經(jīng)營(yíng)范圍是儀器儀表，擁有一支專業(yè)技術(shù)團(tuán)隊(duì)和良好的市場(chǎng)口碑。英賽斯致力于為客戶提供良好的蛋白純化色譜系統(tǒng)，凝膠凈化色譜系統(tǒng)，制備液相色譜，柱塞泵，一切以用戶需求為中心，深受廣大客戶的歡迎。公司將不斷增強(qiáng)企業(yè)重點(diǎn)競(jìng)爭(zhēng)力，努力學(xué)習(xí)行業(yè)知識(shí)，遵守行業(yè)規(guī)范，植根于儀器儀表行業(yè)的發(fā)展。英賽斯秉承“客戶為尊、服務(wù)為榮、創(chuàng)意為先、技術(shù)為實(shí)”的經(jīng)營(yíng)理念，全力打造公司的重點(diǎn)競(jìng)爭(zhēng)力。</p></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/1947032.html</p></div></div></div></div></div><div id="8wcopw8" class="page-right twoColRt"><div id="wahyard" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="ud8nz3m" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" alt="福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></div><p>福建ZF配件哪家強(qiáng) 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