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clearfix"><h1>四川樣品前處理蛋白純化系統(tǒng) 蘇州英賽斯智能科技供應</h1><div id="kiphjla" class="proBig"><div id="8lspmt7" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UpLoadFile/szyss/2020/1/637138171935864644/6371381719358646441189533.jpg" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="vzli8m3" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UpLoadFile/szyss/2020/1/637138171935864644/6371381719358646441189533.jpg" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="hv3eq8f" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>江蘇省</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>四川樣品前處理蛋白純化系統(tǒng),蛋白純化系統(tǒng)</p><p><span>***更新：</span>2020-12-12 23:14:05</p><p><span id="click" >瀏覽次數(shù)：19次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="uol28qi" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">蘇州英賽斯智能科技有限公司</a></p><p><span>聯(lián)系人：</span>李大為</p><p><span>郵箱: </span>570745634@qq.com</p><p><span>電話: </span>18606243033</p><p><span>傳真: </span>0512_63937379</p><p><span>網(wǎng)址: </span>http://www.inscinstech.com.cn</p><p><span>手機: </span>0512-63937378</p><p><span>地址: </span>吳江經(jīng)濟技術(shù)開發(fā)區(qū)聯(lián)楊路南側(cè)、龍橋路西側(cè)</p><p>[當前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="7n2s7dk" class="page-proshow02"><p class="title">詳細說明</p><div id="l8s3cra" class="box"><p><p style="text-indent:25px">蛋白質(zhì)分離純化步驟（二） 2.4.3. 親和層析親和層析（affinity-chromatography）分離技術(shù)是根據(jù)許多蛋白質(zhì)對特定的化學基團具有專一性結(jié)合的原理。這些能被生物大分子如蛋白質(zhì)所識別并與之結(jié)合的基團稱為配基或配體（ligand）。親和層析是一種極有效的分離純化蛋白質(zhì)的方法。例如酶對它的底物具有特殊的親和力；抗原和抗體互為配基。以伴刀豆球蛋白A（concanavalin A）的分離純化為例，由于該蛋白對葡萄糖有專一性親和吸附，因此可把葡萄糖通過適當?shù)幕瘜W反應共價地連接到像瓊脂糖凝膠一類的載體表面上。為了防止載體表面的空間位阻影響待分離的蛋白質(zhì)大分子與其配基的結(jié)合，在配基和載體之間往往插入一段所謂的連接臂（或稱為間隔臂，spacerarm），使配體與載體之間保持足夠的距離。將這種多糖顆粒裝入一定規(guī)格的玻璃管中就制成了一根親和層析柱，四川樣品前處理蛋白純化系統(tǒng)。當含有伴刀豆球蛋白的提取液加到層析柱的上部，四川樣品前處理蛋白純化系統(tǒng)，并沿柱從上往下過時，四川樣品前處理蛋白純化系統(tǒng)，待純化的蛋白質(zhì)與其特異性配基結(jié)合而被吸附到柱上，其他蛋白因不能與葡萄糖配基結(jié)合將通過柱子而流出（圖2-28a）。然后采用一定的洗脫條件，如濃的葡萄糖溶液進行洗脫，即可把該蛋白質(zhì)洗脫下來，達到與其它蛋白質(zhì)分離的目的。四川樣品前處理蛋白純化系統(tǒng)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="四川樣品前處理蛋白純化系統(tǒng),蛋白純化系統(tǒng)" src="http://tyunfile.71360.com/UpLoadFile/szyss/2020/1/637138171935864644/6371381719358646441189533.jpg"></div><p style="text-indent:25px">蛋白質(zhì)分離純化步驟蛋白質(zhì)分離純化的一般原則：大多數(shù)蛋白質(zhì)在組織細胞中都是和核酸等生物分子結(jié)合在一起，而且每種類型的細胞都含有成千上萬種不同的蛋白質(zhì)。許多蛋白質(zhì)在結(jié)構(gòu)、性質(zhì)上有許多相似之處，所以蛋白質(zhì)的分離提純是一項復雜的工作。到目前為止，還沒有一**成的方法能把任何一種蛋白質(zhì)從復雜的混合物中提取出來。但是對于任何一種蛋白質(zhì)都有可能選擇一種較合適的分離純化程序以獲得高純度的制品。且分離的關(guān)鍵步驟、基本手段還是共同的。蛋白質(zhì)提純的目的是增加產(chǎn)品的純度和產(chǎn)量，同時又要保持和提高產(chǎn)品的生物活性。因此，要分離純化某一種蛋白質(zhì)，首先應選擇一種含目的蛋白質(zhì)較豐富的材料。其次，應設法避免蛋白質(zhì)變性，以制備有活性的蛋白質(zhì)。對于大多數(shù)蛋白質(zhì)來說，純化操作都是在0～4℃的低溫下進行的。同時也應避免過酸、過堿的條件以及劇烈的攪拌和振蕩。另外，還要設法除去變性的蛋白質(zhì)和其它雜蛋白，從而達到增加純度和提高產(chǎn)量的目的。四川樣品前處理蛋白純化系統(tǒng)上門維修</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="四川樣品前處理蛋白純化系統(tǒng),蛋白純化系統(tǒng)" src="http://tyunfile.71360.com/UpLoadFile/szyss/2020/1/637138171674741306/6371381716747413062985954.jpg"></div><p style="text-indent:25px">實驗室及中試放大規(guī)模蛋白純化系統(tǒng) Unique Autopure系列蛋白純化系統(tǒng) 主要應用領域： – 蛋白純化系統(tǒng)主要應用于單抗、重組蛋白、疫苗、生化藥、、天然產(chǎn)物和多糖等生物制藥領域。用于生物制品的下游工藝的分離純化。 Unique AutoPure 系列蛋白純化系統(tǒng) ：產(chǎn)品特點及優(yōu)勢： – 模塊化設計，提供多個配置，滿足特殊工藝流程需求 - 低脈動高精度雙柱塞輸液泵，保證優(yōu)異的分離重現(xiàn)性 ; – 全系統(tǒng)與液體接觸的地方均由生物兼容性材料組成、符合FDA、USP VI等相關(guān)法規(guī)要求 ; – DAD全譜直讀檢測器，避免了傳統(tǒng)的機械切換式檢測器所帶來的不穩(wěn)定性及高故障率 ; – 固定波長檢測器燈為長壽命LED燈，壽命大于8000小時，降低維護成本 ; – **技術(shù)紫外檢測器流通池，低死體積、低峰拓展，提高了色譜分離度 ; – 高靈敏度在線pH、電導率、紫外檢測器，低死體積、低峰拓展，提高了色譜分離度 ; – 集中了buffer選擇閥，自動進樣閥、柱位閥等自動化組件，提高了純化的自動化程度 ; – 具有柱前、柱后、壓差報警，氣泡傳感器檢測等功能，極大的保護了系統(tǒng)及層析柱的安全性 ; – 層析工作站軟件是一款功能強大、性能先進、高穩(wěn)定性的色譜數(shù)據(jù)管理系</p><p style="text-indent:25px">蛋白質(zhì)分離純化步驟（二）分離純化蛋白質(zhì)的一般程序 2.1 材料的預處理及細胞破碎分離提純某一種蛋白質(zhì)時，首先要把蛋白質(zhì)從組織或細胞中釋放出來并保持原來的天然狀態(tài)，不喪失活性。所以要采用適當?shù)姆椒▽⒔M織和細胞破碎。常用的破碎組織細胞的方法有： 1. 機械破碎法這種方法是利用機械力的剪切作用，使細胞破碎。常用設備有，高速組織搗碎機、勻漿器、研缽等。 2. 滲透破碎法這種方法是在低滲條件使細胞溶脹而破碎。 3. 反復凍融法生物組織經(jīng)凍結(jié)后，細胞內(nèi)液結(jié)冰膨脹而使細胞脹破。這種方法簡單方便，但要注意那些對溫度變化敏感的蛋白質(zhì)不宜采用此法。 4. 超聲波法使用超聲波震蕩器使細胞膜上所受張力不均而使細胞破碎。 5. 酶法如用溶菌酶破壞微生物細胞等。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="四川樣品前處理蛋白純化系統(tǒng),蛋白純化系統(tǒng)" src="http://tyunfile.71360.com/UpLoadFile/szyss/2020/1/637138171561815352/6371381715618153525073938.jpg"></div><p style="text-indent:25px">蛋白質(zhì)分離純化步驟（二） 2.4 樣品的進一步分離純化用等電點沉淀法、鹽析法所得到的蛋白質(zhì)一般含有其他蛋白質(zhì)雜質(zhì)，須進一步分離提純才能得到有一定純度的樣品。常用的純化方法有：凝膠過濾層析、離子交換纖維素層析、親和層析等等。有時還需要這幾種方法聯(lián)合使用才能得到較高純度的蛋白質(zhì)樣品。 1. 凝膠過濾層析凝膠過濾層析（gel-filtration chromatography）又稱為分子排阻層析或分子篩層析。它是將葡聚糖凝膠（Sephadex）裝入一個柱子中，制成凝膠柱。這種凝膠顆粒具有網(wǎng)狀結(jié)構(gòu)，不同類型凝膠的網(wǎng)孔大小不同。當把蛋白質(zhì)混合樣品加到凝膠柱中時，比凝膠網(wǎng)孔小的蛋白質(zhì)可進入網(wǎng)孔內(nèi)，而大于網(wǎng)孔的分子則不能進入被排阻在凝膠顆粒之外。當用洗脫液洗脫時，被排阻的分子量大的蛋白質(zhì)直接通過凝膠之間的縫隙先被洗脫下來，而比網(wǎng)孔小的蛋白質(zhì)可連續(xù)不斷地進入網(wǎng)孔內(nèi)。這樣的小分子不但流經(jīng)的路程長，而且受到來自凝膠內(nèi)部的阻力也很大，所以蛋白質(zhì)越小，從柱子上洗脫下來所需時間越長。由于不同蛋白質(zhì)的分子大小不同，進入網(wǎng)孔的程度不同，因此流出的速度不同，洗脫所用體積及時間不同，從而達到分離的目的。樣品前處理專業(yè)蛋白純化系統(tǒng)</p><p style="text-indent:25px">四川樣品前處理蛋白純化系統(tǒng)</p><p style="text-indent:25px">三、蛋白質(zhì)分子量的測定 3.3 沉降法沉降法又稱超速離心法。蛋白質(zhì)溶液在受到強大的離心力作用時，蛋白質(zhì)分子趨于下沉，沉降速度與蛋白質(zhì)分子的大小、密度和分子形狀有關(guān)，也與溶劑的密度和粘度有關(guān)。蛋白質(zhì)顆粒在離心場中的沉降速度用每單位時間內(nèi)顆粒下沉的距離來表示。在離心場中，蛋白質(zhì)分子所受到的凈離心力（離心力減去浮力）與溶劑的摩擦力平衡時，每單位離心場強度的沉降速度稱為沉降系數(shù)（Sedimentation Coefficient）。國際上采用Svedberg單位作為沉降系數(shù)的單位，用S表示，以紀念超速離心法的創(chuàng)始人，瑞典***的蛋白質(zhì)化學家T.Svedberg。一個Svedberg單位（或直接稱一個S）為1×10—13s。蛋白質(zhì)的沉降系數(shù)大約在1×10—13～200×10—13s范圍內(nèi)，即1S～200S。四川樣品前處理蛋白純化系統(tǒng)</p><p style="text-indent:25px">蘇州英賽斯智能科技有限公司是一家機器人與自動化設備、機械電子設備的研發(fā)、生產(chǎn)、銷售及技術(shù)咨詢；光學檢測設備、光電產(chǎn)品研發(fā)、制造、銷售；實驗室設備、儀器儀表的研發(fā)、制造、銷售及提供相關(guān)的技術(shù)咨詢和售后服務；自營和代理各類商品及技術(shù)的進出口業(yè)務（限定企業(yè)經(jīng)營或禁止進出口的商品和技術(shù)除外）。（依法需經(jīng)批準的項目，經(jīng)相關(guān)部門批準后方可開展經(jīng)營活動）的公司，是一家集研發(fā)、設計、生產(chǎn)和銷售為一體的專業(yè)化公司。公司自創(chuàng)立以來，投身于蛋白純化色譜系統(tǒng)，凝膠凈化色譜系統(tǒng)，制備液相色譜，柱塞泵，是儀器儀表的主力軍。英賽斯不斷開拓創(chuàng)新，追求出色，以技術(shù)為先導，以產(chǎn)品為平臺，以應用為重點，以服務為保證，不斷為客戶創(chuàng)造更高價值，提供更優(yōu)服務。英賽斯始終關(guān)注儀器儀表行業(yè)。滿足市場需求，提高產(chǎn)品價值，是我們前行的力量。</p></p><br 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