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class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>江蘇轉(zhuǎn)基因熒光定量PCR課題承包</p><p><span>***更新：</span>2020-06-11 05:22:55</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="jtdf4bi" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話(huà): </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線(xiàn)] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="rcris2k" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="fe2p8ce" class="box"><p><p> 實(shí)時(shí)熒光定量PCR具有靈敏度高、特異性高和精確性高的優(yōu)點(diǎn)，此項(xiàng)技術(shù)已被應(yīng)用于分子生物學(xué)、醫(yī)學(xué)、食品檢測(cè)和環(huán)境監(jiān)測(cè)等多個(gè)領(lǐng)域。在分子生物學(xué)領(lǐng)域的研究應(yīng)用定量核酸濃度：傳統(tǒng)方法是用瓊脂糖凝膠電泳或者分光光度計(jì)測(cè)定核酸濃度，其結(jié)果不準(zhǔn)確而且易污染，實(shí)時(shí)熒光定量PCR可以解決這些問(wèn)題，它的準(zhǔn)確性、靈敏度高且無(wú)污染，對(duì)一些傳染性疾病進(jìn)行定量分析、病原微生物和***含量檢測(cè)，江蘇轉(zhuǎn)基因熒光定量PCR課題承包，此項(xiàng)技術(shù)都是優(yōu)先 </p> <p> 研究基因表達(dá)：應(yīng)用實(shí)時(shí)定量PCR技術(shù)可以對(duì)基因時(shí)間、空間表達(dá)水平差異進(jìn)行比較。例如，江蘇轉(zhuǎn)基因熒光定量PCR課題承包，江蘇轉(zhuǎn)基因熒光定量PCR課題承包，對(duì)特定基因用物理、化學(xué)、***等不同方法處理后的差異進(jìn)行比較，為人們的科學(xué)研究提供依據(jù) </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇轉(zhuǎn)基因熒光定量PCR課題承包" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253046152344/6852880.png"></div><p> PCR檢測(cè)方法在臨床醫(yī)學(xué)及實(shí)驗(yàn)室檢驗(yàn)中**有價(jià)值的應(yīng)用領(lǐng)域就是對(duì)***性疾病的診斷。理論上，只要樣本有一個(gè)病原體存在，PCR方法就可以檢測(cè)到。該方法對(duì)病原體的檢測(cè)解決了免疫學(xué)檢測(cè)的“窗口期”問(wèn)題，常用于疾病的早期診斷，可判斷疾病是否處于隱性或亞臨床狀態(tài)。 </p> <p> 大量的熒光定量PCR研究資料表明，病原體數(shù)量與***性疾病病情的輕重程度、傳染性及***效果均有相關(guān)性。因此，通過(guò)熒光定量PCR方法得到的檢測(cè)結(jié)果，一定程度上可以準(zhǔn)確反映疾病***的情況。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇轉(zhuǎn)基因熒光定量PCR課題承包" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253043593750/8100830.png"></div><p style="text-indent:25px">PCR 反應(yīng)的效率也會(huì)影響 Ct 值。在低效率條件下進(jìn)行連續(xù)稀釋擴(kuò)增，與高效率條件下相比，可能會(huì)產(chǎn)生斜率不同的標(biāo)準(zhǔn)曲線(xiàn)。在圖 5 中，兩個(gè)樣品（X 和 Y）分別在低效率和高效率條件下擴(kuò)增，在靶濃度相同的條件下得到了不同的 Ct 值。在此示例中，盡管高效率條件（圖 5 中的藍(lán)色曲線(xiàn)）在高濃度時(shí)產(chǎn)生的 Ct 值更晚，但它在靶濃度低時(shí)卻更為靈敏。PCR 效率取決于實(shí)驗(yàn)、預(yù)混液性能和樣品質(zhì)量。通常情況下，反應(yīng)效率在 90-110% 之間都是可以接受的。另一方面，假定所有其他因素如儀器、試劑和實(shí)驗(yàn)完全相同，該效率也有助于得出***個(gè)樣品的模板量更少的結(jié)論。然而，如果產(chǎn)生兩個(gè) Ct值使用的是不同的儀器、試劑、引物和探針或反應(yīng)體積，該結(jié)論就不成立。因此，只有在使用上文定義的相同反應(yīng)條件來(lái)比較實(shí)驗(yàn)時(shí)，Ct ***值的比較才有意義。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇轉(zhuǎn)基因熒光定量PCR課題承包" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253047636719/652001.png"></div></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/220708.html</p></div></div></div></div></div><div id="th4lk3a" class="page-right twoColRt"><div id="lu4oq33" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="z8aeizq" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" alt="福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)" class="vcenter" 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