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onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="zdv28gs" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253049365234/1538935.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="w7blxwf" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>上海病原微生物熒光定量PCR課題承包</p><p><span>***更新：</span>2020-06-11 10:26:40</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="v8kmt74" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機: </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="kovpbq4" class="page-proshow02"><p class="title">詳細說明</p><div id="drogyv9" class="box"><p><p style="text-indent:25px">Rn 值是由 Applied Biosystems? FAM? 染料的熒光除以 ROX 染料的熒光而計算得出的比率。因此，假定 FAM 染料產(chǎn)生的熒光信號保持不變，ROX 染料量越少，上海病原微生物熒光定量PCR課題承包，產(chǎn)生的 Rn 值就越高。這將導致 Rn 基線上升，以及隨后 ΔRn 的減少和 Ct 值的變化。通過降低 ROX 染料水平而得到不同 Ct 值，上海病原微生物熒光定量PCR課題承包，沒有對反應(yīng)的真實靈敏度產(chǎn)生任何影響，卻有著意想不到的后果。如圖 4 所示，上海病原微生物熒光定量PCR課題承包，降低 ROX 染料的濃度可能會增加 Ct 值的標準差。標準差越大，分辨靶濃度之間的細微區(qū)別的可信度就越低（參加下文的精確度一節(jié)）。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海病原微生物熒光定量PCR課題承包" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253049365234/1538935.png"></div><p style="text-indent:25px">PCR 反應(yīng)的效率也會影響 Ct 值。在低效率條件下進行連續(xù)稀釋擴增，與高效率條件下相比，可能會產(chǎn)生斜率不同的標準曲線。在圖 5 中，兩個樣品（X 和 Y）分別在低效率和高效率條件下擴增，在靶濃度相同的條件下得到了不同的 Ct 值。在此示例中，盡管高效率條件（圖 5 中的藍色曲線）在高濃度時產(chǎn)生的 Ct 值更晚，但它在靶濃度低時卻更為靈敏。PCR 效率取決于實驗、預(yù)混液性能和樣品質(zhì)量。通常情況下，反應(yīng)效率在 90-110% 之間都是可以接受的。另一方面，假定所有其他因素如儀器、試劑和實驗完全相同，該效率也有助于得出***個樣品的模板量更少的結(jié)論。然而，如果產(chǎn)生兩個 Ct值使用的是不同的儀器、試劑、引物和探針或反應(yīng)體積，該結(jié)論就不成立。因此，只有在使用上文定義的相同反應(yīng)條件來比較實驗時，Ct ***值的比較才有意義。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海病原微生物熒光定量PCR課題承包" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253043593750/2901163.png"></div><p style="text-indent:25px">使用可高壓處理的移液器，由于移液器容易受產(chǎn)物氣溶膠或標本 DNA 的污染，因此要使用可高壓處理的移液器。嚴格按照試驗的分區(qū)進行操作，按照提取區(qū)、擴增區(qū)、檢測區(qū)單方向流動，物品、樣品和試劑不可逆向流動。操作多份樣品時，制備反應(yīng)混合液，先將熒光 PCR 反應(yīng)液、熒光探針和酶混合好，然后分裝到每個 PCR 管中，這樣即可以減少操作次數(shù)，避免污染，又可以增加反應(yīng)的精確度。加樣品、加蛋白酶 K 和加樣品過程中，特別注意防止樣品的交叉污染和酶的降解。操作時設(shè)立陰性及陽性對照，可驗證熒光 PCR反應(yīng)的準確性和可信性</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海病原微生物熒光定量PCR課題承包" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253047636719/652001.png"></div></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/225166.html</p></div></div></div></div></div><div id="bzb2zau" class="page-right twoColRt"><div id="lfm3suo" class="box01 margin10 clearfix"><h3><a 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