浙江智能蛋白純化系統(tǒng)制造廠家蘇州英賽斯智能科技供應(yīng)-b2b網(wǎng)站_b2b電子商務(wù)平臺【買產(chǎn)品】-上?？谄蚴称酚邢薰?/title><meta name="description" content="蛋白質(zhì)分離純化步驟（二）2.2蛋白質(zhì)的抽提通常選擇適當?shù)木彌_液溶劑把蛋白質(zhì)提取出來。抽提所用緩沖液的pH、離子強度、組成成分等條件的選擇應(yīng)根據(jù)欲制備的蛋白質(zhì)的性質(zhì)而定。如膜蛋白的抽提，抽提緩沖液中一般要加入表面活性劑（十二烷基磺酸鈉、tri"><meta name="keywords" content="浙江智能蛋白純化系統(tǒng)制造廠家,蛋白純化系統(tǒng)"><link rel="canonical" href="http://www.cdcfah.com/"/><meta name="applicable-device" content="pc"><link rel="shortcut icon" href="/static/new_net_01/images/favicon.ico"><link rel="stylesheet" href="/static/new_net_01/css/slick.css" /><link rel="stylesheet" href="/static/new_net_01/css/addnew.css"><link rel="stylesheet" href="/static/new_net_01/css/sidenav.css"><link rel="stylesheet" href="/static/new_net_01/css/style.css" /><link rel="stylesheet" href="/static/new_net_01/css/tab.css"><link rel="stylesheet" href="/static/new_net_01/css/404.css"> <meta name="baidu-site-verification" content="OBVzrC9aaQ" /> <meta http-equiv="Cache-Control" 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onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="cbyvczi" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UpLoadFile/szyss/2020/1/637138171759963463/6371381717599634633342360.jpg" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="ds3aah4" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>江蘇省</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>浙江智能蛋白純化系統(tǒng)制造廠家,蛋白純化系統(tǒng)</p><p><span>***更新：</span>2020-12-09 01:03:01</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="rp4olic" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">蘇州英賽斯智能科技有限公司</a></p><p><span>聯(lián)系人：</span>李大為</p><p><span>郵箱: </span>570745634@qq.com</p><p><span>電話: </span>18606243033</p><p><span>傳真: </span>0512_63937379</p><p><span>網(wǎng)址: </span>http://www.inscinstech.com.cn</p><p><span>手機: </span>0512-63937378</p><p><span>地址: </span>吳江經(jīng)濟技術(shù)開發(fā)區(qū)聯(lián)楊路南側(cè)、龍橋路西側(cè)</p><p>[當前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="cqfstk9" class="page-proshow02"><p class="title">詳細說明</p><div id="cqkr338" class="box"><p><p style="text-indent:25px">蛋白質(zhì)分離純化步驟（二） 2.2 蛋白質(zhì)的抽提通常選擇適當?shù)木彌_液溶劑把蛋白質(zhì)提取出來。抽提所用緩沖液的pH、離子強度、組成成分等條件的選擇應(yīng)根據(jù)欲制備的蛋白質(zhì)的性質(zhì)而定。如膜蛋白的抽提，抽提緩沖液中一般要加入表面活性劑（十二烷基磺酸鈉、tritonX-100等），使膜結(jié)構(gòu)破壞，利于蛋白質(zhì)與膜分離。在抽提過程中，應(yīng)注意溫度，避免劇烈攪拌等，以防止蛋白質(zhì)的變性。 2，浙江智能蛋白純化系統(tǒng)制造廠家.3蛋白質(zhì)粗制品的獲得選用適當?shù)姆椒▽⑺牡鞍踪|(zhì)與其它雜蛋白分離開來。比較方便的有效方法是根據(jù)蛋白質(zhì)溶解度的差異進行的分離。常用的有下列幾種方法： 1. 等電點沉淀法不同蛋白質(zhì)的等電點不同，可用等電點沉淀法使它們相互分離。 2. 鹽析法不同蛋白質(zhì)鹽析所需要的鹽飽和度不同，所以可通過調(diào)節(jié)鹽濃度將目的蛋白沉淀析出。被鹽析沉淀下來的蛋白質(zhì)仍保持其天然性質(zhì)，并能再度溶解而不變性。 3. 有機溶劑沉淀法中性有機溶劑如乙醇、**，它們的介電常數(shù)比水低，浙江智能蛋白純化系統(tǒng)制造廠家。能使大多數(shù)球狀蛋白質(zhì)在水溶液中的溶解度降低，進而從溶液中沉淀出來，因此可用來沉淀蛋白質(zhì)。此外，有機溶劑會破壞蛋白質(zhì)表面的水化層，促使蛋白質(zhì)分子變得不穩(wěn)定而析出，浙江智能蛋白純化系統(tǒng)制造廠家。由于有機溶劑會使蛋白質(zhì)變性，使用該法時，要注意在低溫下操作.浙江智能蛋白純化系統(tǒng)制造廠家</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="浙江智能蛋白純化系統(tǒng)制造廠家,蛋白純化系統(tǒng)" src="http://tyunfile.71360.com/UpLoadFile/szyss/2020/1/637138171759963463/6371381717599634633342360.jpg"></div><p style="text-indent:25px">蛋白質(zhì)分離純化步驟（二） 2.4.3. 親和層析親和層析（affinity-chromatography）分離技術(shù)是根據(jù)許多蛋白質(zhì)對特定的化學基團具有專一性結(jié)合的原理。這些能被生物大分子如蛋白質(zhì)所識別并與之結(jié)合的基團稱為配基或配體（ligand）。親和層析是一種極有效的分離純化蛋白質(zhì)的方法。例如酶對它的底物具有特殊的親和力；抗原和抗體互為配基。以伴刀豆球蛋白A（concanavalin A）的分離純化為例，由于該蛋白對葡萄糖有專一性親和吸附，因此可把葡萄糖通過適當?shù)幕瘜W反應(yīng)共價地連接到像瓊脂糖凝膠一類的載體表面上。為了防止載體表面的空間位阻影響待分離的蛋白質(zhì)大分子與其配基的結(jié)合，在配基和載體之間往往插入一段所謂的連接臂（或稱為間隔臂，spacerarm），使配體與載體之間保持足夠的距離。將這種多糖顆粒裝入一定規(guī)格的玻璃管中就制成了一根親和層析柱。當含有伴刀豆球蛋白的提取液加到層析柱的上部，并沿柱從上往下過時，待純化的蛋白質(zhì)與其特異性配基結(jié)合而被吸附到柱上，其他蛋白因不能與葡萄糖配基結(jié)合將通過柱子而流出（圖2-28a）。然后采用一定的洗脫條件，如濃的葡萄糖溶液進行洗脫，即可把該蛋白質(zhì)洗脫下來，達到與其它蛋白質(zhì)分離的目的。浙江智能蛋白純化系統(tǒng)制造廠家</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="浙江智能蛋白純化系統(tǒng)制造廠家,蛋白純化系統(tǒng)" src="http://tyunfile.71360.com/UpLoadFile/szyss/2020/1/637138172066720957/6371381720667209577791021.jpg"></div><p style="text-indent:25px">蛋白質(zhì)分離純化步驟（二） 2.4 樣品的進一步分離純化用等電點沉淀法、鹽析法所得到的蛋白質(zhì)一般含有其他蛋白質(zhì)雜質(zhì)，須進一步分離提純才能得到有一定純度的樣品。常用的純化方法有：凝膠過濾層析、離子交換纖維素層析、親和層析等等。有時還需要這幾種方法聯(lián)合使用才能得到較高純度的蛋白質(zhì)樣品。 1. 凝膠過濾層析凝膠過濾層析（gel-filtration chromatography）又稱為分子排阻層析或分子篩層析。它是將葡聚糖凝膠（Sephadex）裝入一個柱子中，制成凝膠柱。這種凝膠顆粒具有網(wǎng)狀結(jié)構(gòu)，不同類型凝膠的網(wǎng)孔大小不同。當把蛋白質(zhì)混合樣品加到凝膠柱中時，比凝膠網(wǎng)孔小的蛋白質(zhì)可進入網(wǎng)孔內(nèi)，而大于網(wǎng)孔的分子則不能進入被排阻在凝膠顆粒之外。當用洗脫液洗脫時，被排阻的分子量大的蛋白質(zhì)直接通過凝膠之間的縫隙先被洗脫下來，而比網(wǎng)孔小的蛋白質(zhì)可連續(xù)不斷地進入網(wǎng)孔內(nèi)。這樣的小分子不但流經(jīng)的路程長，而且受到來自凝膠內(nèi)部的阻力也很大，所以蛋白質(zhì)越小，從柱子上洗脫下來所需時間越長。由于不同蛋白質(zhì)的分子大小不同，進入網(wǎng)孔的程度不同，因此流出的速度不同，洗脫所用體積及時間不同，從而達到分離的目的。</p><p style="text-indent:25px">實驗室及中試放大規(guī)模蛋白純化系統(tǒng) Unique Autopure系列蛋白純化系統(tǒng) 主要應(yīng)用領(lǐng)域： – 蛋白純化系統(tǒng)主要應(yīng)用于單抗、重組蛋白、疫苗、生化藥、、天然產(chǎn)物和多糖等生物制藥領(lǐng)域。用于生物制品的下游工藝的分離純化。 Unique AutoPure 系列蛋白純化系統(tǒng) ：產(chǎn)品特點及優(yōu)勢： – 模塊化設(shè)計，提供多個配置，滿足特殊工藝流程需求 - 低脈動高精度雙柱塞輸液泵，保證優(yōu)異的分離重現(xiàn)性 ; – 全系統(tǒng)與液體接觸的地方均由生物兼容性材料組成、符合FDA、USP VI等相關(guān)法規(guī)要求 ; – DAD全譜直讀檢測器，避免了傳統(tǒng)的機械切換式檢測器所帶來的不穩(wěn)定性及高故障率 ; – 固定波長檢測器燈為長壽命LED燈，壽命大于8000小時，降低維護成本 ; – **技術(shù)紫外檢測器流通池，低死體積、低峰拓展，提高了色譜分離度 ; – 高靈敏度在線pH、電導(dǎo)率、紫外檢測器，低死體積、低峰拓展，提高了色譜分離度 ; – 集中了buffer選擇閥，自動進樣閥、柱位閥等自動化組件，提高了純化的自動化程度 ; – 具有柱前、柱后、壓差報警，氣泡傳感器檢測等功能，極大的保護了系統(tǒng)及層析柱的安全性 ; – 層析工作站軟件是一款功能強大、性能先進、高穩(wěn)定性的色譜數(shù)據(jù)管理系</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="浙江智能蛋白純化系統(tǒng)制造廠家,蛋白純化系統(tǒng)" src="http://tyunfile.71360.com/UpLoadFile/szyss/2020/1/637138172377738528/6371381723777385289828518.jpg"></div><p style="text-indent:25px">三、蛋白質(zhì)分子量的測定蛋白質(zhì)分子量測定的方法很多，目前常用的方法有以下幾種。 3.1 凝膠過濾法凝膠過濾法測定蛋白質(zhì)分子量的原理如“分離純化方法”中所述。一定型號的凝膠顆粒上具有一定大小的孔隙，只允許較小的分子進入膠粒，而大于孔隙的分子則不能進入膠粒而被排阻在膠粒外面。用洗脫液洗脫時，被排阻的分子量大的蛋白質(zhì)先被洗脫下來，隨后能夠進入顆粒的蛋白質(zhì)也按分子量大小而先后被洗脫下來，分子量越小的越后被洗脫下來。由于不同排阻范圍的葡聚糖凝膠有一特定的蛋白質(zhì)分子量范圍，在此范圍內(nèi)，分子量的對數(shù)和洗脫體積之間成線性關(guān)系。因此，用幾種已知分子量的蛋白質(zhì)為標準進行層析分析，以每種蛋白質(zhì)的洗脫體積對它們的分子量的對數(shù)作圖，繪制出標準洗脫曲線。未知蛋白質(zhì)在同樣的條件下進行層析分析，根據(jù)其所用的洗脫體積，從標準洗脫曲線上可求出此未知蛋白質(zhì)對應(yīng)的分子量來。浙江化學分析蛋白純化系統(tǒng)上門維修</p><p style="text-indent:25px">浙江智能蛋白純化系統(tǒng)制造廠家</p><p style="text-indent:25px">蛋白質(zhì)分離純化步驟（二） 2.4.2 纖維素柱層析法該法是利用蛋白質(zhì)的酸堿性質(zhì)作為分離的基礎(chǔ)。離子交換纖維素（cellulose ion-exchanger）是人工合成的纖維素衍生物，它具有松散的親水性網(wǎng)狀結(jié)構(gòu)，有較大的表面積，使蛋白質(zhì)大分子可以自由通過。因此常用于蛋白質(zhì)的分離。（1）羧甲基纖維素（CM-纖維素）在纖維素顆粒上帶有羧甲基基團。在中性pH條件下，羧甲基上的質(zhì)子可解離下來（圖2-27a），而溶液中帶正電荷的蛋白質(zhì)分子可與纖維素顆粒上的羧甲基負電荷結(jié)合。可交換的基團帶正電，因此是一種陽離子交換劑。蛋白質(zhì)與離子交換纖維素之間結(jié)合能力的大小取決于彼此間相反電荷基團之間靜電吸引。 (2）二乙氨基乙基纖維素（DEAE-纖維素）在中性pH條件下，它含有帶正電荷的基團，可與溶液中的帶負電荷的蛋白質(zhì)結(jié)合，可交換的基團帶負電荷，因此是一種陰離子交換劑。當某一蛋白質(zhì)混合溶液通過裝有DEAE-纖維素的層析柱時，帶正電荷的蛋白質(zhì)不能結(jié)合而隨著洗脫液的流動先被洗脫下來。帶負電荷的蛋白質(zhì)將被結(jié)合到柱上。結(jié)合力取決于彼此相反電荷基團間的靜電吸引。然后選用一定pH和離子強度的緩沖液進行洗脫，改變蛋白質(zhì)分子所帶的靜電荷，依次從層析柱流出達到相互分離的目的。浙江智能蛋白純化系統(tǒng)制造廠家</p><p style="text-indent:25px">蘇州英賽斯智能科技有限公司總部位于吳江經(jīng)濟技術(shù)開發(fā)區(qū)聯(lián)楊路南側(cè)、龍橋路西側(cè)，是一家機器人與自動化設(shè)備、機械電子設(shè)備的研發(fā)、生產(chǎn)、銷售及技術(shù)咨詢；光學檢測設(shè)備、光電產(chǎn)品研發(fā)、制造、銷售；實驗室設(shè)備、儀器儀表的研發(fā)、制造、銷售及提供相關(guān)的技術(shù)咨詢和售后服務(wù)；自營和代理各類商品及技術(shù)的進出口業(yè)務(wù)（限定企業(yè)經(jīng)營或禁止進出口的商品和技術(shù)除外）。（依法需經(jīng)批準的項目，經(jīng)相關(guān)部門批準后方可開展經(jīng)營活動）的公司。公司自創(chuàng)立以來，投身于蛋白純化色譜系統(tǒng)，凝膠凈化色譜系統(tǒng)，制備液相色譜，柱塞泵，是儀器儀表的主力軍。英賽斯致力于把技術(shù)上的創(chuàng)新展現(xiàn)成對用戶產(chǎn)品上的貼心，為用戶帶來良好體驗。英賽斯始終關(guān)注自身，在風云變化的時代，對自身的建設(shè)毫不懈怠，高度的專注與執(zhí)著使英賽斯在行業(yè)的從容而自信。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/2321622.html</p></div></div></div></div></div><div id="aeqcqr4" class="page-right twoColRt"><div id="s8cpre4" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="tsubipj" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img 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