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onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="xbjldkm" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>北京*基因法細胞永生化實驗服務(wù),細胞永生化</p><p><span>***更新：</span>2020-06-12 09:15:26</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="bqskr8e" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機: </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="ueg2xmi" class="page-proshow02"><p class="title">詳細說明</p><div id="pjlxe7b" class="box"><p><p> virus基因轉(zhuǎn)染法 </p> <p> 很多virus轉(zhuǎn)染能夠誘導(dǎo)細胞永生化，北京*基因法細胞永生化實驗服務(wù)，例如EBvirus(epstein-barr virus, EBV)、猿猴virus40 (simian virus40, SV40)和人**瘤virus(human papillomavirus, HPV)。這些virus轉(zhuǎn)染其天然宿主細胞，能誘導(dǎo)細胞永生化。 </p> <p> EBV能使B淋巴母細胞永生化形成淋巴母細胞樣細胞系，它是通過潛伏蛋白activate細胞因子與其受體相互作用的途徑使細胞永生的。EBV永生化B細胞的一個**明顯的特點是端粒酶活性的提高，這可能是導(dǎo)致B細胞永生的主要原因。目前，EBvirus介導(dǎo)的細胞永生化應(yīng)用**多的是B淋巴細胞，北京*基因法細胞永生化實驗服務(wù)，其他細胞中的應(yīng)用很少。 </p> <p> SV40是簡單的真核細胞virus，SV40的T抗原片段是**常用的目的片段，將其整合入靶細胞核內(nèi)并表達，北京*基因法細胞永生化實驗服務(wù)，可導(dǎo)致細胞增殖活力的改變并表現(xiàn)出多種與**相關(guān)的轉(zhuǎn)化顯型。應(yīng)用的方法包括：野生型SV40virus共培養(yǎng)轉(zhuǎn)染靶細胞、磷酸鈣法、電穿孔以及逆轉(zhuǎn)錄virus載體法等。北京*基因法細胞永生化實驗服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="北京*基因法細胞永生化實驗服務(wù),細胞永生化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256592626953/2952123.png"></div><p> 建株方法續(xù)4、棄上清液，再加6ml1640全培養(yǎng)液進行第二次洗滌，離心1500轉(zhuǎn)15分鐘。5、棄上清液，加入2ml1640全培養(yǎng)基（全培養(yǎng)基加入前，置37度水浴10分鐘），然后加入環(huán)胞霉素A（4%）和，充分混勻后移入到25平方厘米的培養(yǎng)瓶，置37度，5%CO2培養(yǎng)箱中。6、每2-3天加入3ml新鮮配制的培養(yǎng)基（1MHEPES緩沖液；15%胎牛血清（FBS）；1%青霉素和鏈霉素；PRMI1640補足到***）。7天左右鏡下觀察，可見淋巴細胞明顯增大，出現(xiàn)聚集現(xiàn)象。7、如果細胞增長慢，或細胞密度低，或培養(yǎng)液pH值呈酸性，吸出半量培養(yǎng)液，進行等量換液。8、當(dāng)總量達到14ml時轉(zhuǎn)移到75ml培養(yǎng)瓶中，每2-3周加入5-10ml新鮮培養(yǎng)基。9、約6-8周，當(dāng)總量達到45ml時，置50ml離心管中，離心1500轉(zhuǎn)，10分鐘。棄上清，加入3ml凍存培養(yǎng)基（5%二甲基亞楓(dimethylsufoxide)，95%FBS）混勻，成細胞懸浮液。凍存管分裝，1ml/管，立即置零下80度，1-2小時后移入液氮罐中。北京*基因法細胞永生化實驗服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="北京*基因法細胞永生化實驗服務(wù),細胞永生化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256586035156/2235082.png"></div><p> HSAS4，規(guī)格：>5×100000cells/瓶Cs-(004)-006833大長舌菓蝠肺細胞；DBL1，規(guī)格：>5×100000cells/瓶Cs-(004)-006834恒河猴肺細胞；RM-L1，規(guī)格：>5×100000cells/瓶Cs-(004)-006835小鼠肺成纖維細胞；WML2，規(guī)格：>5×100000cells/瓶Cs-(004)-006836小鼠胚胎細胞；BABL/C-E，規(guī)格：>5×100000cells/瓶Cs-(004)-006837大長舌菓蝠肌肉細胞；DBM1，規(guī)格：>5×100000cells/瓶Cs-(004)-006838豚鼠肺細胞；GP-F1，規(guī)格：>5×100000cells/瓶Cs-(004)-006839豚鼠皮膚細胞；GP-S1，規(guī)格：>5×100000cells/瓶Cs-(004)-006840豚鼠皮膚細胞；GP-S2，規(guī)格：>5×100000cells/瓶Cs-(004)-006841豚鼠心肌細胞；GP-H1，規(guī)格：>5×100000cells/瓶Cs-(004)-006842豚鼠皮膚細胞；GP-S3，規(guī)格：>5×100000cells/瓶Cs-(004)-006843敘利亞倉鼠肌肉細胞；GH-M1，規(guī)格：>5×100000cells/瓶Cs-(004)-006844敘利亞倉鼠皮膚細胞；GH-S1，規(guī)格：>5×100000cells/瓶Cs-(004)-006845大鼠肺細胞；WTRL1，規(guī)格：>5×100000cells/瓶Cs-(004)-006846小耳豬肺細胞；SEP-L1，規(guī)格：>5×100000cells/瓶Cs-(004)-006847小耳豬肺細胞；SEP-L2，規(guī)格：>。</p><p> 細胞永生化(cell immortalization) 指體外培養(yǎng)的細胞經(jīng)過自發(fā)的或受外界因素的影響從增殖衰老危機中逃離, 從而具有無限增殖能力的過程. 自發(fā)永生化的幾率非常小, 嚙齒類動物為10-5~10-6, 而人類細胞則更為罕見, 小于10-12. 通過基因轉(zhuǎn)染等技術(shù)將外源性永生化基因, 如、原*基因和抑*基因突變體等, 導(dǎo)入目的細胞內(nèi), 以增加永生化的發(fā)生率, 進而建立永生化細胞株(immortalized cell strains), 以達到使體外培養(yǎng)的細胞具有無限增殖能力且細胞間無差異的目的。細胞永生化的研究意義：了解細胞增殖與衰老的分子機制；為研究**、控制**細胞增殖以及移植等奠定基礎(chǔ)；可使傳代困難、增殖慢、易衰老的細胞獲得永生，獲得更多的細胞資源。SV40 Large T （SV40大T抗原）的溫度敏感突變株tsA58表達的蛋白在允許溫度（permissive temperature）33℃下有活性，在非允許溫度（non-permissive temperature）39℃下失活，因而用SV40 Large T (tsA58) 永生化的細胞系具有永生化和溫控性雙重特性。 </p> <p> <br /> </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="北京*基因法細胞永生化實驗服務(wù),細胞永生化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256583134765/3971833.png"></div><p> 5×100000cells/瓶Cs-(004)-006819EB***轉(zhuǎn)化的人B淋巴細胞（拉祜族）；KM9406，規(guī)格：>5×100000cells/瓶Cs-(004)-006820EB***轉(zhuǎn)化的人B淋巴細胞（彝族）；HYL，規(guī)格：>5×100000cells/瓶Cs-(004)-006821人胚肺二倍體細胞；HEL-2，規(guī)格：>5×100000cells/瓶Cs-(004)-006822馬鐵菊頭蝠皮膚細胞；GHBS3，規(guī)格：>5×100000cells/瓶Cs-(004)-006823EB***轉(zhuǎn)化的人B淋巴細胞；KM933，規(guī)格：>5×100000cells/瓶Cs-(004)-006824EB***轉(zhuǎn)化的人B淋巴細胞；KM9401，規(guī)格：>5×100000cells/瓶Cs-(004)-006825EB***轉(zhuǎn)化的人B淋巴細胞；KM9402，規(guī)格：>5×100000cells/瓶Cs-(004)-006826EB***轉(zhuǎn)化的人B淋巴細胞；KM9801，規(guī)格：>5×100000cells/瓶Cs-(004)-006827EB***轉(zhuǎn)化的人B淋巴細胞；KM9803，規(guī)格：>5×100000cells/瓶Cs-(004)-006828EB***轉(zhuǎn)化的人B淋巴細胞；KM0501，規(guī)格：>5×100000cells/瓶Cs-(004)-006829人皮膚成纖維細胞；HSAS1，規(guī)格：>5×100000cells/瓶Cs-(004)-006830人皮膚成纖維細胞；HSAS2，規(guī)格：>5×100000cells/瓶Cs-(004)-006831人皮膚成纖維細胞；HSAS3，規(guī)格：>5×100000cells/瓶Cs-(004)-006832人皮膚成纖維細胞。上海EBV法細胞永生化技術(shù)服務(wù)</p><p style="text-indent:25px">北京*基因法細胞永生化實驗服務(wù)</p><p> 原代細胞、干細胞、免疫細胞材料原代細胞取材于原代細胞的永生化的細胞系可以用作更復(fù)雜的生物系統(tǒng)的簡單模型，例如，永生化的神經(jīng)細胞系可以用于研究神經(jīng)病模型中的神經(jīng)元發(fā)育和功能恢復(fù)。干細胞干細胞已被證明可以提供大量可再生的細胞。然而，正如對干細胞所期望的那樣，在培養(yǎng)中進行有限數(shù)量的細胞分裂后，干細胞**終將經(jīng)歷生長停滯和衰老。這限制了它們在生物技術(shù)領(lǐng)域和依賴于大規(guī)模，高通量分析的藥理研究中的開發(fā)。因此，永生化的干細胞提供了克服這些限制的初步嘗試，從而導(dǎo)致了永生化的干細胞系的建立。永生化的人類MSC可以繼續(xù)產(chǎn)生血管生成因子和細胞因子，例如HGF和VEGF。免疫細胞免疫療法是一種**療法，可增強人體對***癥的天然防御能力。永生化是產(chǎn)生大量免疫細胞進行***的比較好方法。例如，Wuetal.開發(fā)了一種建立永生化和組成型活化的人原代血液樹突細胞系的方法，并探索了對樹突細胞的各種修飾以增強其免疫***潛力。B細胞的永生化是單克隆抗體研究方面的熱點，通過B細胞的永生化，可以為篩選一些功能特殊的抗體和病原特異性單克隆抗體提供條件。同種屬來源的單克隆抗體能有效克服異源抗體產(chǎn)生的免疫排斥，從而為疾病的預(yù)防和***起到不可替代的作用。北京*基因法細胞永生化實驗服務(wù)</p><p style="text-indent:25px">上海朝瑞生物科技有限公司創(chuàng)辦于2003-01-22，是一家服務(wù)型的公司。經(jīng)過多年不斷的歷練探索和創(chuàng)新發(fā)展，上海朝瑞科技是一家有限責(zé)任公司企業(yè)，一直貫徹“以人為本，服務(wù)于社會”的經(jīng)營理念;“質(zhì)量高速，誠守信譽，持續(xù)發(fā)展”的質(zhì)量方針。公司業(yè)務(wù)涵蓋[ "科研技術(shù)服務(wù)", "應(yīng)用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學(xué)產(chǎn)品銷售" 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