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class="wrap clearfix"><section class="container clearfix tcPro"><section class="home-main container clearfix"><div id="u88dkho" class="twoCol"><div id="xjgikhj" class="page-proshow clearfix"><p class="current"> 當前位置:<a href="http://www.cdcfah.com/">首頁?<a href="http://www.cdcfah.com/cp/">產(chǎn)品供應</a>?<a href="http://www.cdcfah.com/cp/second-_nongye/">農(nóng)業(yè)</a>?<a href="http://www.cdcfah.com/cp/third-nongye_nlmyxmhz/">農(nóng)林牧漁項目合作</a>?上海五色免疫組化技術(shù)服務(wù) 歡迎來電上海朝瑞生物科技供應</p><div id="8v8hjv3" class="leftbox clearfix"><h1>上海五色免疫組化技術(shù)服務(wù) 歡迎來電上海朝瑞生物科技供應</h1><div id="tsfhega" class="proBig"><div id="rgiu8km" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750107422/8042334.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="mb3cjac" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750107422/8042334.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="8tsol2m" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>上海五色免疫組化技術(shù)服務(wù),免疫組化</p><p><span>***更新：</span>2020-06-12 10:16:11</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="wvnzw33" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機: </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="esj8t7d" class="page-proshow02"><p class="title">詳細說明</p><div id="rgyvskr" class="box"><p><p> 提高抗原檢測率。常用的修復方法從強到弱一般分為三種，高壓修復、微波修復、胰酶修復。修復液也分為若干種（具體的可以查閱相關(guān)資料，大量的：中性的、高pH的等）。我們實驗室一般用微波修復中火6min*4次，效果不錯。注意微波修復后自然冷卻30min左右（只要你覺得修復液的溫度達室溫即可）。5）細胞通透目的是使抗體能夠充分地進入胞內(nèi)進行結(jié)合反應。一般用TritonX-100、蛋白酶k等通透液。如TritonX-100可以溶解細胞膜，上海五色免疫組化技術(shù)服務(wù)、細胞核膜、細胞器膜上的脂質(zhì)而使抗體及大分子結(jié)構(gòu)的物質(zhì)進入胞漿和胞核內(nèi)，故在細胞免疫組化時尤為推薦使用，這樣抗體就能順利進入胞內(nèi)與相應抗原結(jié)合。在免疫組織化學（>10um厚切片）和免疫細胞化學中一般用TritonX-100作為細胞通透劑，在膜上打孔。同時也是一種去污劑，一般在PBS中加入后終濃度是，而前者終濃度是。石蠟切片4um左右可以不通透，因為細胞已經(jīng)被切開了。6）滅活內(nèi)源性過氧化物酶和生物素在傳統(tǒng)的ABC法和SP法中，免疫組化反應結(jié)果容易收到內(nèi)源性過氧化物酶和生物素的干擾，必須用過氧化氫和卵白素等進行滅活，上海五色免疫組化技術(shù)服務(wù)。滅活內(nèi)源性POD一般3%過氧化氫滅活時間短點，可以10min左右，上海五色免疫組化技術(shù)服務(wù)，而，一般10~30min。上海五色免疫組化技術(shù)服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海五色免疫組化技術(shù)服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750107422/8042334.png"></div><p style="text-indent:25px"> 近年來，隨著免疫組織化學技術(shù)的發(fā)展和各種特異性抗體的出現(xiàn)，使許多疑難**得到了明確診斷。在常規(guī)**病理診斷中，5%-10%的病例單靠H.E.染色難以作出明確的形態(tài)學診斷。尤其是免疫組化在**診斷和鑒別診斷中的實用價值受到了普遍的認可，其在低分化或未分化**的鑒別診斷時，準確率可達50%-75%。免疫組織化學的臨床應用主要包括以下幾方面：⑴惡性**的診斷與鑒別診斷；⑵確定轉(zhuǎn)移性惡性**的原發(fā)部位；⑶對某類**進行進一步的病理分型；⑷軟組織**的zhiliao一般需根據(jù)正確的組織學分類，因其種類多、組織形態(tài)相像，有時難以區(qū)分其組織來源，應用多種標志進行免疫組化研究對軟組織**的診斷是不可缺少的；⑸發(fā)現(xiàn)微小轉(zhuǎn)移灶，有助于臨床zhiliao方案的確定，包括手術(shù)范圍的確定。⑹為臨床提供zhiliao方案的選擇。江蘇多色熒光免疫組化技術(shù)服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海五色免疫組化技術(shù)服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899752167969/2304287.png"></div><p> 乳化劑的鑒定·判斷乳化是否充分，可將一滴乳化好的液體滴在水面上(冷水中)，如能長時間保持圓珠形而不散開，表示乳化達到要求。(3)免疫方法——免疫途徑·常有靜脈、腹腔、肌肉、皮下、皮內(nèi)、淋巴結(jié)、腳掌等注射。·一般采用多點注射方法–常在足、掌、腋窩淋巴結(jié)周圍、背部兩側(cè)、頜下、耳后等處的皮內(nèi)或皮下。–皮內(nèi)易引起細胞免疫反應，有利于提高抗體的效價。(3)免疫方法——免疫途徑(續(xù))·幾點說明：–大動物一般不用腹腔注射–顆?？乖褪褂米魟r不能靜脈注射–抗原寶貴可采用淋巴結(jié)內(nèi)微量注射法，只需10～100?g抗原即可獲得較好的免疫效果。–皮內(nèi)注射較困難，特別是天冷時更難注入。(3)免疫方法——次數(shù)及間隔時間·次數(shù)一般為2～3次(初次免疫和加強免疫)–***注射后，10～15天再加強注射；–劑量同***或為***的一半，用不全佐劑或不用佐劑。·間隔時間，一般而言，動物越大，間隔越長–豚鼠、大鼠為7～8天，兔子為10～15天，羊為14～28天；–第三次注射的間隔時間更長些，效果更好。舉例1：家兔的免疫·初次免疫–用50～200?gAg加入FCA，在背部皮下注射6～8點，每點～，也可肌肉內(nèi)或皮內(nèi)注射；·兩周后，加強免疫–將50～200?gAg于PBS或FIA中。</p><p style="text-indent:25px">2. 免疫酶細胞化學技術(shù)是目前免疫組織化學研究中**常用的技術(shù)．基本原理是先以酶標記的抗體與組織或細胞作用，然后加入酶的底物，生成有色的不溶性產(chǎn)物或具有一定電子密度的顆粒，通過光鏡或電鏡，對細胞或組織內(nèi)的相應抗原進行定位或定性研究．3. 免疫膠體金技術(shù)就是用膠體金標記一抗，二抗或其他的能特異性的結(jié)合免疫球蛋白的分子（如葡萄球菌A蛋白）等作為探針對組織或細胞內(nèi)的抗原進行定性，定位或定量研究．由于膠體金的電子密度高，多用于免疫電鏡的單標記或多標記的定位研究．</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海五色免疫組化技術(shù)服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899751181641/619319.png"></div><p> 6、看來主要禍根是抗體稀釋液的pH值出問題了，于是我又用PBS替代抗體稀釋液和新試劑盒內(nèi)的試劑對組織切片做了免疫組化，結(jié)果還是沒有做出來：背景很深。這真把我搞慘了。難道還有什么原因嗎？通過仔細分析：一抗一定是結(jié)合上去了（老SP試劑盒結(jié)果很好），問題是二抗沒有結(jié)合上去也不對（因為***背景很深，這說明二抗可能也結(jié)合上去了），DAB孵育時間現(xiàn)在只用1、5min也是背景深而特異性染色淺，那我又懷疑封閉血清了。7、我做了兩張切片：一張用老試劑盒內(nèi)還剩下一點的血清而另一張用新試劑盒內(nèi)的封閉血清，其余試劑（二抗、SP）均用新試劑盒提供的，結(jié)果是前一張效果很好，而后一張能夠看到特異性染色，但背景太深，拍照效果不佳?？磥矸忾]血清也是罪會禍首之一。結(jié)果分析顯示：抗體稀釋液的PH值偏低和封閉血清的失效導致了我的免疫組化結(jié)果的不佳，望大家在以后的組化實驗中也要注意這兩個不太引人注意的關(guān)鍵問題。慘痛的教訓，值得引以為鑒。案例二DAB染色后切片著色呈陰性結(jié)果背景：其實免疫組化在DAB染色后一般有四種情況：陽性染色效果很好、陰性染色、非特異性染色很深、陰陽臉（染色不均勻）。而陰性染色是許多初學者經(jīng)常遇到的頭疼問題。江蘇多色熒光免疫組化技術(shù)服務(wù)</p><p style="text-indent:25px">上海五色免疫組化技術(shù)服務(wù)</p><p> pH)漂洗5min×3/次，不時震蕩(洗去多余游離的熒光素標記的抗體)?！ぞ彌_甘油封片–分析純無熒光的甘油9份+pH，。·鏡檢：在熒光顯微鏡下觀察。·優(yōu)點：方法簡便、特異性高，非特異性熒光染色少?！と秉c：敏感性偏低；而且每檢查一種抗原就需要制備一種熒光抗體。若檢測多種抗原需制備多種相應的熒光標記抗體。?直接免疫熒光法的注意事項·對熒光標記的抗體的稀釋：要保證抗體的蛋白有一定的濃度；·一般稀釋度不應超過1:20，抗體濃度過低，會導致產(chǎn)生的熒光過弱，影響結(jié)果的觀察。?直接免疫熒光法的注意事項(續(xù))·染色溫度和時間需要根據(jù)各種不同的標本及抗原而變化；–染色時間：從10min到數(shù)小時，一般30min；–染色溫度：多采用室溫(25?C)，高于37?C可加強染色效果，但對不耐熱的抗原(如流行性乙型腦炎***)可采用0-2?C的低溫，延長染色時間。–低溫染色過夜較37?C30min效果好的多。?直接免疫熒光法的注意事項(續(xù))·試驗時需設(shè)置下列對照：–自發(fā)熒光對照(空白對照)：標本加，。–陽性對照：用已知的陽性標本加熒光標記的特異性抗體。–特異性對照(***試驗)：標本加未標記的特異性抗體，再加熒光標記的特異性抗體。上海五色免疫組化技術(shù)服務(wù)</p><p style="text-indent:25px">上海朝瑞生物科技有限公司致力于化工，以科技創(chuàng)新實現(xiàn)***管理的追求。上海朝瑞科技擁有一支經(jīng)驗豐富、技術(shù)創(chuàng)新的專業(yè)研發(fā)團隊，以高度的專注和執(zhí)著為客戶提供[ "科研技術(shù)服務(wù)", "應用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學產(chǎn)品銷售" ]。公司堅持以技術(shù)創(chuàng)新為發(fā)展引擎，以客戶滿意為動力，目前擁有5~10人專業(yè)人員，年營業(yè)額達到2000-3000萬元。上海朝瑞科技始終關(guān)注化工市場，以敏銳的市場洞察力以準確定位，實現(xiàn)與客戶的成長共贏。</p></p><br /><p>文章來源地址: 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