上海新手RNA提取試劑盒***的選擇來電咨詢上海朝瑞生物科技供應(yīng)-b2b網(wǎng)站_b2b電子商務(wù)平臺(tái)【買產(chǎn)品】-上?？谄蚴称酚邢薰?/title><meta name="description" content="向吸附柱中加入600ul漂洗液(使用前請(qǐng)先檢查是否已加入無水乙醇)，2-812,000rpm℃離心2min，棄廢液。向吸附柱中加入600ul漂洗液，2-812,000rpm℃離心2min，棄廢液。12000rpm離心2min，棄掉收集管，上"><meta name="keywords" content="上海新手RNA提取試劑盒***的選擇,RNA提取試劑盒"><link rel="canonical" href="http://www.cdcfah.com/"/><meta name="applicable-device" content="pc"><link rel="shortcut icon" href="/static/new_net_01/images/favicon.ico"><link rel="stylesheet" href="/static/new_net_01/css/slick.css" /><link rel="stylesheet" href="/static/new_net_01/css/addnew.css"><link rel="stylesheet" href="/static/new_net_01/css/sidenav.css"><link rel="stylesheet" href="/static/new_net_01/css/style.css" /><link rel="stylesheet" href="/static/new_net_01/css/tab.css"><link rel="stylesheet" href="/static/new_net_01/css/404.css"> <meta name="baidu-site-verification" content="OBVzrC9aaQ" /> <meta http-equiv="Cache-Control" content="no-transform" /> <meta 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class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>上海新手RNA提取試劑盒***的選擇,RNA提取試劑盒</p><p><span>***更新：</span>2020-06-12 19:19:06</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="rakwxyk" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="nqw2vb3" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="2zlmst3" class="box"><p><p> 向吸附柱中加入600ul漂洗液(使用前請(qǐng)先檢查是否已加入無水乙醇)，2-8 12,000 rpm ℃ 離心2min，棄廢液。 </p> <p> 向吸附柱中加入600ul漂洗液，2-8 12,000 rpm ℃ 離心2min，棄廢液。 </p> <p> 12000rpm離心2min，棄掉收集管，上海新手RNA提取試劑盒***的選擇，將吸附柱置于室溫放置數(shù)分鐘將吸附柱中殘余的漂洗液去除。 </p> <p> 將吸附柱放入新管中，向膜**滴加50-100ul RNase free ddH2O，室溫放置5min，12000rpm室溫離心2min即得到RNA </p> <p> <p> 所有相關(guān)器皿耗材都應(yīng)為RNase-free產(chǎn)品，操作過程要小心，帶口罩，上海新手RNA提取試劑盒***的選擇，上海新手RNA提取試劑盒***的選擇、手套避免環(huán)境中RNA酶污染樣品。 </p> <p> <br /> </p> 從成立之初，凡知醫(yī)學(xué)就致力于基因快速診斷產(chǎn)品開發(fā)，立志從上游**技術(shù)攻關(guān)實(shí)現(xiàn)進(jìn)口替代。上海新手RNA提取試劑盒***的選擇</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海新手RNA提取試劑盒***的選擇,RNA提取試劑盒" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739423857422/1376989.png"></div><p style="text-indent:25px">通用RNA提取試劑盒是經(jīng)過改進(jìn)開發(fā)的新一代產(chǎn)品，提高了裂解液的裂解能力和提取的靈敏度，同時(shí)通過可在特定適宜的條件下特異、可逆地結(jié)合RNA，而各種蛋白質(zhì)和其他雜質(zhì)均可被去除掉對(duì)硅基質(zhì)膜的改進(jìn)增強(qiáng)了對(duì)RNA的吸附能力，得到的RNA 純度更好，質(zhì)量更高。該試劑盒可從各種細(xì)胞或組織中快速提取總RNA，每個(gè)吸附柱每次可處理50–100 mg 組織或5×106 細(xì)胞，可同時(shí)處理大量不同樣品。一個(gè)小時(shí)內(nèi)即可完成反應(yīng)，提取的總RNA 沒有DNA 和蛋白的污染，可用于Northern blot、Dot blot、polyA 篩選、體外翻譯、RNase 保護(hù)分析和分子克隆。江蘇新手RNA提取試劑盒500T/5865元會(huì)等很長(zhǎng)一段時(shí)間）。普通的提取實(shí)驗(yàn)用國(guó)產(chǎn)的試劑盒就足以。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海新手RNA提取試劑盒***的選擇,RNA提取試劑盒" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116738986650391/4176717.png"></div><p> 樣品勻漿后，加入氯仿前，可在-80°C 凍存放置一個(gè)月。 </p> <p> RNA 沉淀可以保存在 75%乙醇中，2-8°C 放置一周或-20°C 放置一年。 </p> <p> 該試劑盒***于專業(yè)人員的科學(xué)研究使用，不得用于臨床診斷或***，更不得用于食品或藥品中。 </p> <p> 實(shí)驗(yàn)步驟、細(xì)胞裂解或組織勻漿： </p> <p> a貼壁細(xì)胞：吸盡培液，按照比例加入 Trizol 消化細(xì)胞，即每 10 平方厘米細(xì)胞中加入 1 ml Trizol，六孔板每孔加 1 ml Trizol，12 孔板每孔加 0.5 ml Trizol。晃動(dòng) 3-5 下，再用移液器反復(fù)吹打幾下，確保細(xì)胞全部裂解，然后將溶液轉(zhuǎn)移至離心管中?！咀ⅰ考尤?Trizol 量不足時(shí)，會(huì)導(dǎo)致 DNA 污染。 </p><p> 樣品處理 </p> <p> 組織：將組織在液氮中磨碎。每 50–100 mg 組織加1 ml 溶液 RL，用勻漿儀進(jìn)行勻漿處理。樣品體積不應(yīng)超過裂解液RZ 體積的十分之一。 </p> <p> 單層培養(yǎng)細(xì)胞：直接在培養(yǎng)板中加入裂解液RZ 裂解細(xì)胞，每10 cm2 面積加1 ml溶液 RL。用取樣器抽打幾次。 </p> <p> 注意：溶液 RL 的加入量根據(jù)培養(yǎng)瓶面積決定，不是由細(xì)胞數(shù)決定。如果加量不足，可能導(dǎo)致提取的RNA中有DNA污染。 </p> <p> 細(xì)胞懸液：離心取細(xì)胞，棄上清。每5–10×106 動(dòng)物細(xì)胞和植物細(xì)胞加入1 ml 溶液 RL。加溶液 RL 前不要洗滌細(xì)胞，以免降解mRNA。 </p> <p> 將勻漿樣品在15–30℃放置5 min，使得核酸蛋白復(fù)合物完全分離。向吸附柱中加入600ul漂洗液(使用前請(qǐng)先檢查是否已加入無水乙醇)，2-8 12,000 rpm ℃ 離心2min，棄廢液。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海新手RNA提取試劑盒***的選擇,RNA提取試劑盒" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739414511719/6417652.png"></div><p> RNA試劑盒用于各種植物、動(dòng)物、微生物的RNA提取，在每個(gè)試劑盒里都有一份說明使用說明書。實(shí)驗(yàn)者只需要按照說明書上的步驟操作即可。使用時(shí)無需配制其它試劑，非常方便，適合于RNA的快速提取。所提取的RNA完整性好、純度高，可用于分子生物學(xué)后實(shí)驗(yàn)。但較好的試劑盒價(jià)格比較貴，在實(shí)驗(yàn)室可以根據(jù)自己的實(shí)驗(yàn)需要來定奪試劑盒的使用 </p> <p> （以離心柱型為例）：一般包括：成分?jǐn)?shù)量?jī)?chǔ)藏溫度有效期裂解液100ml2-8℃一年漂洗液30mlRT一年洗柱液100mlRT一年RNasefreeddH2O30mlRT一年RNasefree吸附柱100個(gè)RT一年RNasefree收集管(2ml)100個(gè)RT一年此外，還配有一份試劑盒使用說明書，根據(jù)不同的生產(chǎn)商，可能還配有不同的試劑，如：DNA酶、溶菌酶等。價(jià)格不高，基本上各地均有現(xiàn)貨，如天根（國(guó)內(nèi)生產(chǎn)的試劑盒中銷售面比較廣的一種）等。北京四步完成RNA提取試劑盒****</p><p style="text-indent:25px">芯片和試劑，對(duì)操作人員技術(shù)要求低，全程只需20分鐘，患者等待周期短，成本低。上海新手RNA提取試劑盒***的選擇</p><p> Trizol法提取RNA實(shí)驗(yàn)步驟及原理 </p> <p> 1.在提取組織RNA時(shí),每50～100mg組織用1mL Trizol試劑對(duì)組織進(jìn)行裂解；提取細(xì)胞RNA時(shí),先用PBS洗滌/離心沉淀細(xì)胞,每5-10 ╳106個(gè)細(xì)胞加1mL Trizol后, 反復(fù)用吹打或劇烈振蕩以裂解細(xì)胞. </p> <p> <br /> </p> <p> 2、將組織或細(xì)胞的Trizol裂解液轉(zhuǎn)入EP管中, 在室溫15~30℃下放置5分鐘；在EP管中,按照每1mL TRIZOL加0.2mL氯仿的量加入氯仿(即為1/5體積), 蓋上EP管蓋子, 在手中用力震蕩15秒(10次), 在室溫下（15℃～30℃）放置2～3分鐘后, 14000rpm（4℃）離心15分鐘. </p> <p> 【注：離心后分為3層，RNA在上層水相, DNA在中間層, 蛋白質(zhì)在下層有機(jī)相】 </p> <p> <br /> </p> <p> 3、取上層水相置于新EP管中(～500uL, 用200uL的黃色*頭小心吸取, 約吸取3次, 杜絕取到中間層/下層), 按照每1mL Trizol加0.5mL異丙醇的量加入異丙醇(即為1/2體積), 4℃下靜置20分鐘, 沉淀RNA；14000rpm（4℃）離心15分鐘. </p> <p> </p> <p> 4、按照每1mL TRIZOL加1mL75%乙醇進(jìn)行洗滌(即為1倍體積, 注:用DEPC水配置75%乙醇), 混合(#用移液器輕輕吹打即可,不用將沉淀打碎), 14000rpm（4℃）離心20分鐘, 棄上清(#比較好用真空泵吸去上清)；讓沉淀的RNA在室溫下自然干燥(#或在超凈臺(tái)中小風(fēng)吹干,比較好置于干凈的環(huán)境中，防止灰塵雜質(zhì)進(jìn)入)；用45uL Rnase-free water 溶解RNA沉淀. 上海新手RNA提取試劑盒***的選擇</p><p style="text-indent:25px">上海朝瑞生物科技有限公司創(chuàng)建于2003-01-22，注冊(cè)資金 700-1000萬元，辦公設(shè)施齊全，辦公環(huán)境優(yōu)越，已***實(shí)行網(wǎng)絡(luò)化辦公，**提高了速度和效率。在上海朝瑞科技近多年發(fā)展歷史，公司旗下現(xiàn)有品牌zrbiorise,SPRICELL,scienceladde等。公司以用心服務(wù)為**價(jià)值,希望通過我們的專業(yè)水平和不懈努力,將1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長(zhǎng) 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗(yàn)檢測(cè)服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級(jí)改造產(chǎn)品服務(wù) 9.發(fā)布實(shí)驗(yàn)室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項(xiàng)目團(tuán)隊(duì)招聘13.發(fā)布研究生婚戀 14.發(fā)布項(xiàng)目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù)等業(yè)務(wù)進(jìn)行到底。上海朝瑞生物科技有限公司主營(yíng)業(yè)務(wù)涵蓋[ "科研技術(shù)服務(wù)", 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