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class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256581992187/3487203.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="hcmbjs3" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256581992187/3487203.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="kgl81iq" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>江蘇HPV法細(xì)胞永生化實(shí)驗(yàn)服務(wù),細(xì)胞永生化</p><p><span>***更新：</span>2020-06-13 08:15:33</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="wsxqbub" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="onc3djt" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="0ocl37u" class="box"><p><p> virus基因轉(zhuǎn)染法 </p> <p> 很多virus轉(zhuǎn)染能夠誘導(dǎo)細(xì)胞永生化，例如EBvirus(epstein-barr virus, EBV)、猿猴virus40 (simian virus40, SV40)和人**瘤virus(human papillomavirus, HPV)。這些virus轉(zhuǎn)染其天然宿主細(xì)胞，能誘導(dǎo)細(xì)胞永生化。 </p> <p> EBV能使B淋巴母細(xì)胞永生化形成淋巴母細(xì)胞樣細(xì)胞系，它是通過(guò)潛伏蛋白activate細(xì)胞因子與其受體相互作用的途徑使細(xì)胞永生的。EBV永生化B細(xì)胞的一個(gè)**明顯的特點(diǎn)是端粒酶活性的提高，這可能是導(dǎo)致B細(xì)胞永生的主要原因。目前，EBvirus介導(dǎo)的細(xì)胞永生化應(yīng)用**多的是B淋巴細(xì)胞，其他細(xì)胞中的應(yīng)用很少。 </p> <p> SV40是簡(jiǎn)單的真核細(xì)胞virus，江蘇HPV法細(xì)胞永生化實(shí)驗(yàn)服務(wù)，SV40的T抗原片段是**常用的目的片段，將其整合入靶細(xì)胞核內(nèi)并表達(dá)，可導(dǎo)致細(xì)胞增殖活力的改變并表現(xiàn)出多種與**相關(guān)的轉(zhuǎn)化顯型，江蘇HPV法細(xì)胞永生化實(shí)驗(yàn)服務(wù)。應(yīng)用的方法包括：野生型SV40virus共培養(yǎng)轉(zhuǎn)染靶細(xì)胞，江蘇HPV法細(xì)胞永生化實(shí)驗(yàn)服務(wù)、磷酸鈣法、電穿孔以及逆轉(zhuǎn)錄virus載體法等。江蘇HPV法細(xì)胞永生化實(shí)驗(yàn)服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇HPV法細(xì)胞永生化實(shí)驗(yàn)服務(wù),細(xì)胞永生化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256581992187/3487203.png"></div><p> 細(xì)胞永生化(cell immortalization) 指體外培養(yǎng)的細(xì)胞經(jīng)過(guò)自發(fā)的或受外界因素的影響從增殖衰老危機(jī)中逃離, 從而具有無(wú)限增殖能力的過(guò)程. 自發(fā)永生化的幾率非常小, 嚙齒類動(dòng)物為10-5~10-6, 而人類細(xì)胞則更為罕見(jiàn), 小于10-12. 通過(guò)基因轉(zhuǎn)染等技術(shù)將外源性永生化基因, 如、原*基因和抑*基因突變體等, 導(dǎo)入目的細(xì)胞內(nèi), 以增加永生化的發(fā)生率, 進(jìn)而建立永生化細(xì)胞株(immortalized cell strains), 以達(dá)到使體外培養(yǎng)的細(xì)胞具有無(wú)限增殖能力且細(xì)胞間無(wú)差異的目的。細(xì)胞永生化的研究意義：了解細(xì)胞增殖與衰老的分子機(jī)制；為研究**、控制**細(xì)胞增殖以及移植等奠定基礎(chǔ)；可使傳代困難、增殖慢、易衰老的細(xì)胞獲得永生，獲得更多的細(xì)胞資源。SV40 Large T （SV40大T抗原）的溫度敏感突變株tsA58表達(dá)的蛋白在允許溫度（permissive temperature）33℃下有活性，在非允許溫度（non-permissive temperature）39℃下失活，因而用SV40 Large T (tsA58) 永生化的細(xì)胞系具有永生化和溫控性雙重特性。 </p> <p> <br /> 江蘇HPV法細(xì)胞永生化實(shí)驗(yàn)服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇HPV法細(xì)胞永生化實(shí)驗(yàn)服務(wù),細(xì)胞永生化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256597792968/4214172.png"></div><p style="text-indent:25px">細(xì)胞永生化(cell immortalization) 指體外培養(yǎng)的細(xì)胞經(jīng)過(guò)自發(fā)的或受外界因素的影響從增殖衰老危機(jī)中逃離, 從而具有無(wú)限增殖能力的過(guò)程. 自發(fā)永生化的幾率非常小, 嚙齒類動(dòng)物為10-5~10-6, 而人類細(xì)胞則更為罕見(jiàn), 小于10-12. 通過(guò)基因轉(zhuǎn)染等技術(shù)將外源性永生化基因, 如***、原*基因和抑*基因突變體等, 導(dǎo)入目的細(xì)胞內(nèi), 以增加永生化的發(fā)生率, 進(jìn)而建立永生化細(xì)胞株(immortalized cell strains), 以達(dá)到使體外培養(yǎng)的細(xì)胞具有無(wú)限增殖能力且細(xì)胞間無(wú)差異的目的。</p><p> 這些細(xì)胞仍然具有相應(yīng)的表型和穩(wěn)定的核型。而使用原*基因?qū)е录?xì)胞永生化的，細(xì)胞往往會(huì)發(fā)生核型變化，特別是當(dāng)細(xì)胞被多次傳代以后。通過(guò)hTERT介導(dǎo)的永生化細(xì)胞可以觀察到以下特性：1、正常細(xì)胞周期控制；2、接觸***；3、錨定依賴；4、保留對(duì)血清和有絲分裂原的正常生長(zhǎng)反應(yīng)；5、需要生長(zhǎng)因子進(jìn)行增殖；6、具有正常核型；7、沒(méi)有與轉(zhuǎn)化相關(guān)的變化，如致瘤性，可以在軟瓊脂上生長(zhǎng)。hTERT介導(dǎo)的永生化細(xì)胞系結(jié)合了原代細(xì)胞的生理屬性和細(xì)胞系的長(zhǎng)期培養(yǎng)特性，同時(shí)避免前者的衰老和后者的不穩(wěn)定核型。此外，在許多研究中，hTERT介導(dǎo)永生化細(xì)胞可以誘導(dǎo)分化為不同的細(xì)胞，這些分化后的細(xì)胞具有特定組織特性并表達(dá)分化細(xì)胞特異性蛋白，并可以形成在只有在體內(nèi)才能形成的某型結(jié)構(gòu)。ATCC人端粒酶逆轉(zhuǎn)錄酶（hTERT）永生化細(xì)胞系**了細(xì)胞生物學(xué)研究的一個(gè)重大突破，聯(lián)合了原代細(xì)胞的生化屬性以及傳統(tǒng)細(xì)胞系長(zhǎng)期培養(yǎng)的特性。?即使在高代次，也能夠維持細(xì)胞在體內(nèi)的生物學(xué)特性；?生命周期是原代細(xì)胞的5倍；?基本維持二倍體核型；?與原代細(xì)胞比較，基因表達(dá)基本不發(fā)生變化；?沒(méi)有批間差；?減少實(shí)驗(yàn)結(jié)果的不一致性。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇HPV法細(xì)胞永生化實(shí)驗(yàn)服務(wù),細(xì)胞永生化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256592958984/8981407.png"></div><p> 細(xì)胞永生化端粒酶法 </p> <p> 端粒酶是一種能延長(zhǎng)端粒末端的核糖**白酶，由RNA和蛋白質(zhì)組成。端粒酶能以自身的RNA為模板，從端粒DNA3’-OH 末端延伸端?；蚝铣尚碌亩肆NA，以補(bǔ)償細(xì)胞分裂時(shí)染色體末端的縮短，從而維持端粒的長(zhǎng)度，使細(xì)胞不會(huì)因端粒耗盡而出現(xiàn)凋亡。正常情況下，大多數(shù)體細(xì)胞端粒酶的表達(dá)是嚴(yán)格調(diào)控的，表達(dá)是瞬時(shí)和低水平的。在原代培養(yǎng)的細(xì)胞中穩(wěn)定表達(dá)人端粒酶催化亞基(hTERT)基因能穩(wěn)定端粒的長(zhǎng)度，使細(xì)胞易于永生化，因此hTERT的activate是細(xì)胞永生化的重要步驟。雖然activate端粒酶活性能夠使多種人和動(dòng)物的細(xì)胞永生化，但是對(duì)于有些細(xì)胞，重建端粒酶活性并不足以使細(xì)胞永生化，還需要借助*基因法。上海細(xì)胞永生化哪家專業(yè)</p><p style="text-indent:25px">江蘇HPV法細(xì)胞永生化實(shí)驗(yàn)服務(wù)</p><p> 這并不令人吃驚雖然大多數(shù)**似乎需要端粒酶變得永生化，但已知只有約10％至20％的**在端粒酶基因上游的啟動(dòng)子中發(fā)生單核苷酸變化。然而，這包括大約70％的黑色素瘤和50％的肝和膀胱*。Hockemeyer說(shuō)，用來(lái)支持這種TERT啟動(dòng)子突變上調(diào)端粒酶表達(dá)的理論的證據(jù)一直是自相矛盾的：*細(xì)胞往往具有較短端粒的染色體，但具有更高水平的端粒酶，這種高水平應(yīng)當(dāng)會(huì)產(chǎn)生更長(zhǎng)的端粒。根據(jù)這些新的發(fā)現(xiàn)，端粒在*前細(xì)胞（precancerouscell）中比較短，這是因?yàn)槎肆Ｃ竸偤米阋跃S持而不延長(zhǎng)端粒。Hockemeyer說(shuō)，“我們的論文調(diào)和了關(guān)于攜帶這種突變的**的相互矛盾的信息。”該發(fā)現(xiàn)還解決了另外一個(gè)**近的違反直覺(jué)的發(fā)現(xiàn)：具有更短端粒的人更能抵抗黑色素瘤。他說(shuō)，其中的原因在于如果一種TERT啟動(dòng)子突變會(huì)促進(jìn)*前病灶（痣）向黑色素瘤轉(zhuǎn)化，那么在具有更短端粒的人中，在細(xì)胞上調(diào)表達(dá)端粒酶和讓它們變得永生化之前，這些細(xì)胞將會(huì)死掉。該研究還涉及對(duì)由人多能性干細(xì)胞分化的細(xì)胞進(jìn)行基因改造，使之?dāng)y帶TERT啟動(dòng)子突變，隨后追蹤它們變得永生化的進(jìn)展過(guò)程。這些結(jié)果與從加州大學(xué)舊金山分校海倫迪勒家族綜合**中心的患者體內(nèi)獲得的人皮膚病灶樣品中觀察到的進(jìn)展過(guò)程是相同的。江蘇HPV法細(xì)胞永生化實(shí)驗(yàn)服務(wù)</p><p style="text-indent:25px">上海朝瑞生物科技有限公司坐落在上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室，是一家專業(yè)的1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長(zhǎng) 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗(yàn)檢測(cè)服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級(jí)改造產(chǎn)品服務(wù) 9.發(fā)布實(shí)驗(yàn)室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項(xiàng)目團(tuán)隊(duì)招聘13.發(fā)布研究生婚戀 14.發(fā)布項(xiàng)目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù)公司。目前我公司在職員工達(dá)到5~10人人，是一個(gè)有活力有能力有創(chuàng)新精神的高效團(tuán)隊(duì)。公司業(yè)務(wù)范圍主要包括：[ "科研技術(shù)服務(wù)", "應(yīng)用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學(xué)產(chǎn)品銷售" ]等。公司奉行顧客至上、質(zhì)量為首、的經(jīng)營(yíng)宗旨，深受客戶好評(píng)。公司憑著雄厚的技術(shù)力量、飽滿的工作態(tài)度、扎實(shí)的工作作風(fēng)、良好的職業(yè)道德，樹(shù)立了優(yōu)良的[ "科研技術(shù)服務(wù)", "應(yīng)用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學(xué)產(chǎn)品銷售" ]形象，贏得了社會(huì)各界的信任和認(rèn)可。</p></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/243564.html</p></div></div></div></div></div><div id="5cgv0z3" class="page-right twoColRt"><div id="7a0ged9" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="st9jhgk" class="recomPic likePro"><a 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