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#f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>四川專業(yè)蛋白純化系統(tǒng)制造廠家,蛋白純化系統(tǒng)</p><p><span>***更新：</span>2020-12-17 18:08:57</p><p><span id="click" >瀏覽次數(shù)：4次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="2o2jlnu" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">蘇州英賽斯智能科技有限公司</a></p><p><span>聯(lián)系人：</span>李大為</p><p><span>郵箱: </span>570745634@qq.com</p><p><span>電話: </span>18606243033</p><p><span>傳真: </span>0512_63937379</p><p><span>網(wǎng)址: </span>http://www.inscinstech.com.cn</p><p><span>手機(jī): </span>0512-63937378</p><p><span>地址: </span>吳江經(jīng)濟(jì)技術(shù)開發(fā)區(qū)聯(lián)楊路南側(cè)、龍橋路西側(cè)</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="y78qikr" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="2r4vsz8" class="box"><p><p> 蛋白質(zhì)純化方法-親和層析親和層析法包括特異性配體親和層析法以及通用性配體親和層析法。比較這兩種親和層析法，四川專業(yè)蛋白純化系統(tǒng)制造廠家。特異性配體親和層析法通常將復(fù)雜的生命大分子物質(zhì)作為配體，它的結(jié)合力比較大河吸附選擇性比較強(qiáng)。而通用性配體親和層析法通常將簡(jiǎn)單的小分子物質(zhì)作為配體，因此，它具有低廉的成本，吸附容量比較高，通過(guò)對(duì)吸附和脫附條件的改善能夠使得層析的分辨率提高。原理親和層析的原理類似于酶-底物，四川專業(yè)蛋白純化系統(tǒng)制造廠家，***-受體與抗原-抗體等特異性反應(yīng)的機(jī)理。有一定的親和力在每對(duì)反應(yīng)物之間。與在酶與底物的反應(yīng)中，四川專業(yè)蛋白純化系統(tǒng)制造廠家，只有特異的底物才可以結(jié)合一定的酶，使復(fù)合物產(chǎn)生相同。在親和層析中，只有特異的配體才可以和一定的生命大分子有親和力，并且使復(fù)合物產(chǎn)生。親和層析配體呈固相，而酶與底物的反應(yīng)底物呈液相是它們的不同點(diǎn)。事實(shí)上，親和層析是在含有活化基團(tuán)的基質(zhì)M上以共價(jià)鍵的方式固化具有識(shí)別能力的配體L，將固相載體制作成功而固化后的配體束縛特異物質(zhì)的能力依然保持。所以當(dāng)在小層析柱裝入固相載體，使得欲分離的樣品在該柱經(jīng)過(guò)。此時(shí)，樣品中對(duì)配體有親和力的物質(zhì)S就能夠通過(guò)結(jié)構(gòu)互補(bǔ)效應(yīng)、范德瓦爾力與靜電引力等作用在固相載體上吸附著。四川專業(yè)蛋白純化系統(tǒng)制造廠家</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="四川專業(yè)蛋白純化系統(tǒng)制造廠家,蛋白純化系統(tǒng)" src="http://tyunfile.71360.com/UpLoadFile/szyss/2020/1/637138171561815352/6371381715618153525073938.jpg"></div><p style="text-indent:25px">實(shí)驗(yàn)室及中試放大規(guī)模蛋白純化系統(tǒng) Unique Autopure系列蛋白純化系統(tǒng) 主要應(yīng)用領(lǐng)域： – 蛋白純化系統(tǒng)主要應(yīng)用于單抗、重組蛋白、疫苗、生化藥、、天然產(chǎn)物和多糖等生物制藥領(lǐng)域。用于生物制品的下游工藝的分離純化。 Unique AutoPure 系列蛋白純化系統(tǒng) ：產(chǎn)品特點(diǎn)及優(yōu)勢(shì)： – 模塊化設(shè)計(jì)，提供多個(gè)配置，滿足特殊工藝流程需求 - 低脈動(dòng)高精度雙柱塞輸液泵，保證優(yōu)異的分離重現(xiàn)性 ; – 全系統(tǒng)與液體接觸的地方均由生物兼容性材料組成、符合FDA、USP VI等相關(guān)法規(guī)要求 ; – DAD全譜直讀檢測(cè)器，避免了傳統(tǒng)的機(jī)械切換式檢測(cè)器所帶來(lái)的不穩(wěn)定性及高故障率 ; – 固定波長(zhǎng)檢測(cè)器燈為長(zhǎng)壽命LED燈，壽命大于8000小時(shí)，降低維護(hù)成本 ; – **技術(shù)紫外檢測(cè)器流通池，低死體積、低峰拓展，提高了色譜分離度 ; – 高靈敏度在線pH、電導(dǎo)率、紫外檢測(cè)器，低死體積、低峰拓展，提高了色譜分離度 ; – 集中了buffer選擇閥，自動(dòng)進(jìn)樣閥、柱位閥等自動(dòng)化組件，提高了純化的自動(dòng)化程度 ; – 具有柱前、柱后、壓差報(bào)警，氣泡傳感器檢測(cè)等功能，極大的保護(hù)了系統(tǒng)及層析柱的安全性 ; – 層析工作站軟件是一款功能強(qiáng)大、性能先進(jìn)、高穩(wěn)定性的色譜數(shù)據(jù)管理系四川智能蛋白純化系統(tǒng)制造廠家</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="四川專業(yè)蛋白純化系統(tǒng)制造廠家,蛋白純化系統(tǒng)" src="http://tyunfile.71360.com/UpLoadFile/szyss/2020/1/637138171347973309/6371381713479733098214210.jpg"></div><p style="text-indent:25px">三、蛋白質(zhì)分子量的測(cè)定 3.3 沉降法沉降法又稱超速離心法。蛋白質(zhì)溶液在受到強(qiáng)大的離心力作用時(shí)，蛋白質(zhì)分子趨于下沉，沉降速度與蛋白質(zhì)分子的大小、密度和分子形狀有關(guān)，也與溶劑的密度和粘度有關(guān)。蛋白質(zhì)顆粒在離心場(chǎng)中的沉降速度用每單位時(shí)間內(nèi)顆粒下沉的距離來(lái)表示。在離心場(chǎng)中，蛋白質(zhì)分子所受到的凈離心力（離心力減去浮力）與溶劑的摩擦力平衡時(shí)，每單位離心場(chǎng)強(qiáng)度的沉降速度稱為沉降系數(shù)（Sedimentation Coefficient）。國(guó)際上采用Svedberg單位作為沉降系數(shù)的單位，用S表示，以紀(jì)念超速離心法的創(chuàng)始人，瑞典***的蛋白質(zhì)化學(xué)家T.Svedberg。一個(gè)Svedberg單位（或直接稱一個(gè)S）為1×10—13s。蛋白質(zhì)的沉降系數(shù)大約在1×10—13～200×10—13s范圍內(nèi)，即1S～200S。</p><p style="text-indent:25px">蛋白質(zhì)分離純化步驟（二） 2.4 樣品的進(jìn)一步分離純化用等電點(diǎn)沉淀法、鹽析法所得到的蛋白質(zhì)一般含有其他蛋白質(zhì)雜質(zhì)，須進(jìn)一步分離提純才能得到有一定純度的樣品。常用的純化方法有：凝膠過(guò)濾層析、離子交換纖維素層析、親和層析等等。有時(shí)還需要這幾種方法聯(lián)合使用才能得到較高純度的蛋白質(zhì)樣品。 1. 凝膠過(guò)濾層析凝膠過(guò)濾層析（gel-filtration chromatography）又稱為分子排阻層析或分子篩層析。它是將葡聚糖凝膠（Sephadex）裝入一個(gè)柱子中，制成凝膠柱。這種凝膠顆粒具有網(wǎng)狀結(jié)構(gòu)，不同類型凝膠的網(wǎng)孔大小不同。當(dāng)把蛋白質(zhì)混合樣品加到凝膠柱中時(shí)，比凝膠網(wǎng)孔小的蛋白質(zhì)可進(jìn)入網(wǎng)孔內(nèi)，而大于網(wǎng)孔的分子則不能進(jìn)入被排阻在凝膠顆粒之外。當(dāng)用洗脫液洗脫時(shí)，被排阻的分子量大的蛋白質(zhì)直接通過(guò)凝膠之間的縫隙先被洗脫下來(lái)，而比網(wǎng)孔小的蛋白質(zhì)可連續(xù)不斷地進(jìn)入網(wǎng)孔內(nèi)。這樣的小分子不但流經(jīng)的路程長(zhǎng)，而且受到來(lái)自凝膠內(nèi)部的阻力也很大，所以蛋白質(zhì)越小，從柱子上洗脫下來(lái)所需時(shí)間越長(zhǎng)。由于不同蛋白質(zhì)的分子大小不同，進(jìn)入網(wǎng)孔的程度不同，因此流出的速度不同，洗脫所用體積及時(shí)間不同，從而達(dá)到分離的目的。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="四川專業(yè)蛋白純化系統(tǒng)制造廠家,蛋白純化系統(tǒng)" src="http://tyunfile.71360.com/UpLoadFile/szyss/2020/1/637138171759963463/6371381717599634633342360.jpg"></div><p style="text-indent:25px">蛋白質(zhì)分離純化步驟（二） 2.2 蛋白質(zhì)的抽提通常選擇適當(dāng)?shù)木彌_液溶劑把蛋白質(zhì)提取出來(lái)。抽提所用緩沖液的pH、離子強(qiáng)度、組成成分等條件的選擇應(yīng)根據(jù)欲制備的蛋白質(zhì)的性質(zhì)而定。如膜蛋白的抽提，抽提緩沖液中一般要加入表面活性劑（十二烷基磺酸鈉、tritonX-100等），使膜結(jié)構(gòu)破壞，利于蛋白質(zhì)與膜分離。在抽提過(guò)程中，應(yīng)注意溫度，避免劇烈攪拌等，以防止蛋白質(zhì)的變性。 2.3蛋白質(zhì)粗制品的獲得選用適當(dāng)?shù)姆椒▽⑺牡鞍踪|(zhì)與其它雜蛋白分離開來(lái)。比較方便的有效方法是根據(jù)蛋白質(zhì)溶解度的差異進(jìn)行的分離。常用的有下列幾種方法： 1. 等電點(diǎn)沉淀法不同蛋白質(zhì)的等電點(diǎn)不同，可用等電點(diǎn)沉淀法使它們相互分離。 2. 鹽析法不同蛋白質(zhì)鹽析所需要的鹽飽和度不同，所以可通過(guò)調(diào)節(jié)鹽濃度將目的蛋白沉淀析出。被鹽析沉淀下來(lái)的蛋白質(zhì)仍保持其天然性質(zhì)，并能再度溶解而不變性。 3. 有機(jī)溶劑沉淀法中性有機(jī)溶劑如乙醇、**，它們的介電常數(shù)比水低。能使大多數(shù)球狀蛋白質(zhì)在水溶液中的溶解度降低，進(jìn)而從溶液中沉淀出來(lái)，因此可用來(lái)沉淀蛋白質(zhì)。此外，有機(jī)溶劑會(huì)破壞蛋白質(zhì)表面的水化層，促使蛋白質(zhì)分子變得不穩(wěn)定而析出。由于有機(jī)溶劑會(huì)使蛋白質(zhì)變性，使用該法時(shí)，要注意在低溫下操作.四川專業(yè)蛋白純化系統(tǒng)制造廠家</p><p style="text-indent:25px">四川專業(yè)蛋白純化系統(tǒng)制造廠家</p><p style="text-indent:25px">蛋白質(zhì)分離純化步驟（二） 2.4.2 纖維素柱層析法該法是利用蛋白質(zhì)的酸堿性質(zhì)作為分離的基礎(chǔ)。離子交換纖維素（cellulose ion-exchanger）是人工合成的纖維素衍生物，它具有松散的親水性網(wǎng)狀結(jié)構(gòu)，有較大的表面積，使蛋白質(zhì)大分子可以自由通過(guò)。因此常用于蛋白質(zhì)的分離。（1）羧甲基纖維素（CM-纖維素）在纖維素顆粒上帶有羧甲基基團(tuán)。在中性pH條件下，羧甲基上的質(zhì)子可解離下來(lái)（圖2-27a），而溶液中帶正電荷的蛋白質(zhì)分子可與纖維素顆粒上的羧甲基負(fù)電荷結(jié)合?？山粨Q的基團(tuán)帶正電，因此是一種陽(yáng)離子交換劑。蛋白質(zhì)與離子交換纖維素之間結(jié)合能力的大小取決于彼此間相反電荷基團(tuán)之間靜電吸引。 (2）二乙氨基乙基纖維素（DEAE-纖維素）在中性pH條件下，它含有帶正電荷的基團(tuán)，可與溶液中的帶負(fù)電荷的蛋白質(zhì)結(jié)合，可交換的基團(tuán)帶負(fù)電荷，因此是一種陰離子交換劑。當(dāng)某一蛋白質(zhì)混合溶液通過(guò)裝有DEAE-纖維素的層析柱時(shí)，帶正電荷的蛋白質(zhì)不能結(jié)合而隨著洗脫液的流動(dòng)先被洗脫下來(lái)。帶負(fù)電荷的蛋白質(zhì)將被結(jié)合到柱上。結(jié)合力取決于彼此相反電荷基團(tuán)間的靜電吸引。然后選用一定pH和離子強(qiáng)度的緩沖液進(jìn)行洗脫，改變蛋白質(zhì)分子所帶的靜電荷，依次從層析柱流出達(dá)到相互分離的目的。四川專業(yè)蛋白純化系統(tǒng)制造廠家</p><p style="text-indent:25px">蘇州英賽斯智能科技有限公司是一家機(jī)器人與自動(dòng)化設(shè)備、機(jī)械電子設(shè)備的研發(fā)、生產(chǎn)、銷售及技術(shù)咨詢；光學(xué)檢測(cè)設(shè)備、光電產(chǎn)品研發(fā)、制造、銷售；實(shí)驗(yàn)室設(shè)備、儀器儀表的研發(fā)、制造、銷售及提供相關(guān)的技術(shù)咨詢和售后服務(wù)；自營(yíng)和代理各類商品及技術(shù)的進(jìn)出口業(yè)務(wù)（限定企業(yè)經(jīng)營(yíng)或禁止進(jìn)出口的商品和技術(shù)除外）。（依法需經(jīng)批準(zhǔn)的項(xiàng)目，經(jīng)相關(guān)部門批準(zhǔn)后方可開展經(jīng)營(yíng)活動(dòng)）的公司，致力于發(fā)展為創(chuàng)新務(wù)實(shí)、誠(chéng)實(shí)可信的企業(yè)。英賽斯擁有一支經(jīng)驗(yàn)豐富、技術(shù)創(chuàng)新的專業(yè)研發(fā)團(tuán)隊(duì)，以高度的專注和執(zhí)著為客戶提供蛋白純化色譜系統(tǒng)，凝膠凈化色譜系統(tǒng)，制備液相色譜，柱塞泵。英賽斯不斷開拓創(chuàng)新，追求出色，以技術(shù)為先導(dǎo)，以產(chǎn)品為平臺(tái)，以應(yīng)用為重點(diǎn)，以服務(wù)為保證，不斷為客戶創(chuàng)造更高價(jià)值，提供更優(yōu)服務(wù)。英賽斯始終關(guān)注自身，在風(fēng)云變化的時(shí)代，對(duì)自身的建設(shè)毫不懈怠，高度的專注與執(zhí)著使英賽斯在行業(yè)的從容而自信。</p></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/2444702.html</p></div></div></div></div></div><div id="zikcub7" class="page-right twoColRt"><div id="ptl3au7" class="box01 margin10 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