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onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="91xtjp7" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>江蘇lncRNA熒光定量PCR分析服務(wù),熒光定量PCR</p><p><span>***更新：</span>2020-06-14 16:20:40</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="nrfntzf" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="b9jpdjp" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="dj9rp99" class="box"><p><p> 轉(zhuǎn)基因：利用熒光定量PCR技術(shù)將轉(zhuǎn)基因信號(hào)擴(kuò)增放大。 </p> <p> 在環(huán)境監(jiān)測(cè)方面的應(yīng)用實(shí)時(shí)熒光定量PCR技術(shù)已經(jīng)被國(guó)內(nèi)外應(yīng)用在環(huán)境監(jiān)測(cè)中，提高了監(jiān)測(cè)水平。此項(xiàng)技術(shù)可以檢測(cè)河流中環(huán)境微生物隨著季節(jié)變化的情況。還可以用來(lái)對(duì)地表水和飲用水中致病菌進(jìn)行檢測(cè)以便及時(shí)預(yù)告地表水污染情況以及采取相應(yīng)有效措施避免大范圍污染 </p> <p> 儀器擺放：實(shí)時(shí)熒光定量 PCR 儀應(yīng)安放在濕度較低，江蘇lncRNA熒光定量PCR分析服務(wù)，江蘇lncRNA熒光定量PCR分析服務(wù)、灰塵較少，江蘇lncRNA熒光定量PCR分析服務(wù)、遠(yuǎn)離水池的平穩(wěn)臺(tái)面上，不能有強(qiáng)光直射，室內(nèi)應(yīng)通風(fēng)良好，無(wú)腐蝕性氣體江蘇lncRNA熒光定量PCR分析服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇lncRNA熒光定量PCR分析服務(wù),熒光定量PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253049677734/8789186.png"></div><p style="text-indent:25px">SNP基因分型技術(shù)原理是PCR擴(kuò)增含有SNP的基因組片段，主要特點(diǎn)是準(zhǔn)確性高、靈活性強(qiáng)、通量大，主要方法是TaqMan探針法。TaqMan探針法針對(duì)染色體上的不同SNP位點(diǎn)分別設(shè)計(jì)PCR引物和TaqMan探針，進(jìn)行實(shí)時(shí)熒光PCR擴(kuò)增。探針的5’-端和3’-端分別標(biāo)記一個(gè)報(bào)告熒光基團(tuán)和一個(gè)淬滅熒光基團(tuán)。當(dāng)溶液中存在PCR產(chǎn)物時(shí)，該探針與模板退火，即產(chǎn)生了適合于核酸外切酶活性的底物，從而將探針5’-端連接的熒光分子從探針上切割下來(lái)，破壞兩熒光分子間的PRET，發(fā)出熒光。通常用于少量SNP位點(diǎn)分析。江蘇lncRNA熒光定量PCR分析服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇lncRNA熒光定量PCR分析服務(wù),熒光定量PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253043603516/9770963.png"></div><p> 熒光定量PCR 技術(shù)，是通過(guò)在PCR 反應(yīng)體系中加入熒光基團(tuán)，利用熒光信號(hào)的變化實(shí)時(shí)監(jiān)測(cè)PCR 擴(kuò)增反應(yīng)中每一個(gè)循環(huán)擴(kuò)增產(chǎn)物量的變化，對(duì)起始模板進(jìn)行定量分析的方法。實(shí)驗(yàn)采用兩種定量方式，即***定量和相對(duì)定量。熒光定量PCR 可用于mRNA 表達(dá)量水平檢測(cè)、MicroRNA 表達(dá)量水平檢測(cè)和基因拷貝數(shù)檢測(cè)。實(shí)驗(yàn)的方法可分為SYBR Green I 法和TaqMan 探針法。 </p> <p> <br /> </p> <p> 客戶提供提供樣本 </p> <p> 細(xì)胞：細(xì)胞數(shù)>10^6； </p> <p> 組織：總量>100 mg； </p> <p> DNA 或RNA 樣品：總量>2 μg。 </p> <p> 可選樣本引物：可由客戶提供，沒有引物的情況下，可由生工生物設(shè)計(jì)合成 </p> <p> 提供信息 </p> <p> 靶基因信息：包括基因序列或GenBank Accession Number 等；背景資料：包括生物物種信息（Human、Mouse、Rat 等）、DNA/RNA 來(lái)源、基因豐度等。**終交付交付報(bào)告 </p> <p> 實(shí)驗(yàn)報(bào)告：包括實(shí)驗(yàn)方法、步驟、所用試劑、儀器、照片及相關(guān)分析數(shù)據(jù)等。 </p> <p> <br /> </p><p> 熒光定量PCR實(shí)驗(yàn)步驟： </p> <p> 取凍存已裂解的細(xì)胞，室溫放置5分鐘使其完全溶解。 </p> <p> 兩相分離每1ml的TRIZOL試劑裂解的樣品中加入0.2ml的lvfang，蓋緊管蓋。手動(dòng)劇烈振蕩管體15秒后，15到30℃孵育2到3分鐘。4℃下12000rpm離心15分鐘。離心后混合液體將分為下層的紅色酚lvfang相，中間層以及無(wú)色水相上層。RNA全部被分配于水相中。水相上層的體積大約是勻漿時(shí)加入的TRIZOL試劑的60%。 </p> <p> RNA沉淀將水相上層轉(zhuǎn)移到一干凈無(wú)RNA酶的離心管中。加等體積異丙醇混合以沉淀其中的RNA，混勻后15到30℃孵育10分鐘后，于4℃下12000rpm 離心10分鐘。此時(shí)離心前不可見的RNA沉淀將在管底部和側(cè)壁上形成膠狀沉淀塊。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇lncRNA熒光定量PCR分析服務(wù),熒光定量PCR" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253046611328/9318580.png"></div><p> 一個(gè)評(píng)估 PCR 效率的關(guān)鍵參數(shù)是 R2，它是說(shuō)明如何使用一個(gè)數(shù)值預(yù)測(cè)另一個(gè)數(shù)值的相關(guān)程度的統(tǒng)計(jì)學(xué)術(shù)語(yǔ)。如果 R2 為 1，那么 Y 值 (Ct) 可以用來(lái)準(zhǔn)確預(yù)測(cè) X 值（圖7A）。如果 R2 為 0，那么就不能通過(guò) Y 值預(yù)測(cè) X 值（圖 7B）。R2 值 >0.99 表示兩個(gè)數(shù)值之間相關(guān)的置信度良好。 </p> <p> 精確度 </p> <p> 標(biāo)準(zhǔn)差（偏差的平方根）是**常用的精確度計(jì)量方法。如果許多數(shù)據(jù)點(diǎn)都靠**均值，則標(biāo)準(zhǔn)差就??；如果許多數(shù)據(jù)點(diǎn)都遠(yuǎn)離平均值，則標(biāo)準(zhǔn)差就大。 </p> <p> 實(shí)際上，足夠多重復(fù)次數(shù)產(chǎn)生的數(shù)據(jù)**形成大致的正態(tài)分布。這經(jīng)?？赏ㄟ^(guò)經(jīng)典的中心極限定理來(lái)證明，該定理稱大量**同分布隨機(jī)變量的和在無(wú)限多時(shí)趨向于正態(tài)分布。如圖 8A 所示，約 68% 的值在平均值的 1 個(gè)標(biāo)準(zhǔn)差之內(nèi)，約 95% 的值在平均值的 2 個(gè)標(biāo)準(zhǔn)差之內(nèi)，約 99.7% 的值在平均值的 3 個(gè)標(biāo)準(zhǔn)差之內(nèi)。江蘇lncRNA熒光定量PCR分析服務(wù)</p><p style="text-indent:25px">江蘇lncRNA熒光定量PCR分析服務(wù)</p><p> 實(shí)時(shí)熒光定量 PCR，又稱為定量 PCR 或 qPCR，可以為測(cè)定樣品中的靶標(biāo)序列或基因數(shù)量提供簡(jiǎn)單、簡(jiǎn)潔的方法。該方法高度簡(jiǎn)化，有時(shí)會(huì)忽略實(shí)現(xiàn)方法方面的關(guān)鍵因素，因此導(dǎo)致出現(xiàn)問題。本文將重點(diǎn)強(qiáng)調(diào)設(shè)置和評(píng)估實(shí)時(shí)熒光定量 PCR 反應(yīng)時(shí)必須考慮的這些因素。 </p> <p> <br /> 江蘇lncRNA熒光定量PCR分析服務(wù)</p> 實(shí)時(shí)熒光定量 PCR 數(shù)據(jù)的圖形表示。Rn 是報(bào)告基團(tuán)染料的熒光除以惰性參比染料的熒光的比值；即，Rn 是歸一化到 Applied Biosystems? ROX? 染料的熒光信號(hào)的報(bào)告基團(tuán)信號(hào)。(A) 在此視圖中，Rn 根據(jù) PCR 循環(huán)數(shù)進(jìn)行繪制。(B) ΔRn 為 Rn 減去基線；ΔRn 根據(jù) PCR 循環(huán)數(shù)進(jìn)行繪制。(C) 擴(kuò)增圖顯示對(duì)數(shù) (Δrn) 隨 PCR 循環(huán)數(shù)的變化。<p style="text-indent:25px">上海朝瑞生物科技有限公司成立于2003-01-22，是一家服務(wù)型的公司。公司業(yè)務(wù)涵蓋[ "科研技術(shù)服務(wù)", "應(yīng)用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學(xué)產(chǎn)品銷售" ]等，價(jià)格合理，品質(zhì)有保證。公司秉持誠(chéng)信為本的經(jīng)營(yíng)理念，在化工深耕多年，以技術(shù)為先導(dǎo)，以自主產(chǎn)品為**，發(fā)揮人才優(yōu)勢(shì)，打造化工質(zhì)量品牌。截止當(dāng)前，我公司年?duì)I業(yè)額度達(dá)到2000-3000萬(wàn)元，爭(zhēng)取在一公分的領(lǐng)域里做出一公里的深度。</p></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/258312.html</p></div></div></div></div></div><div id="7tj7nvv" class="page-right twoColRt"><div id="jn97dtj" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="fb9f9rx" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img 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