上海9分鐘完成RNA提取試劑盒500T/5865元歡迎來(lái)電上海朝瑞生物科技供應(yīng)-b2b網(wǎng)站_b2b電子商務(wù)平臺(tái)【買產(chǎn)品】-上?？谄蚴称酚邢薰?/title><meta name="description" content="用于各種植物、動(dòng)物、微生物的RNA提取，在每個(gè)試劑盒里都有一份說(shuō)明使用說(shuō)明書(shū)。實(shí)驗(yàn)者只需要按照說(shuō)明書(shū)上的步驟操作即可。使用時(shí)無(wú)需配制其它試劑，非常方便，適合于RNA的快速提取。所提取的RNA完整性好、純度高，可用于分子生物學(xué)后實(shí)驗(yàn)。但較好的"><meta name="keywords" content="上海9分鐘完成RNA提取試劑盒500T/5865元,RNA提取試劑盒"><link rel="canonical" href="http://www.cdcfah.com/"/><meta name="applicable-device" content="pc"><link rel="shortcut icon" href="/static/new_net_01/images/favicon.ico"><link rel="stylesheet" href="/static/new_net_01/css/slick.css" /><link rel="stylesheet" href="/static/new_net_01/css/addnew.css"><link rel="stylesheet" href="/static/new_net_01/css/sidenav.css"><link rel="stylesheet" href="/static/new_net_01/css/style.css" /><link rel="stylesheet" href="/static/new_net_01/css/tab.css"><link rel="stylesheet" href="/static/new_net_01/css/404.css"> <meta name="baidu-site-verification" 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clearfix"><h1>上海9分鐘完成RNA提取試劑盒500T/5865元歡迎來(lái)電上海朝瑞生物科技供應(yīng)</h1><div id="2esosok" class="proBig"><div id="2cymgc2" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739414521485/5765636.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="m4awcqm" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739414521485/5765636.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="gukomsw" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>上海9分鐘完成RNA提取試劑盒500T/5865元,RNA提取試劑盒</p><p><span>***更新：</span>2020-06-16 02:19:34</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="2quayea" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="ko2soc2" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="2s2gmqw" class="box"><p><p> 用于各種植物、動(dòng)物、微生物的RNA提取，在每個(gè)試劑盒里都有一份說(shuō)明使用說(shuō)明書(shū)。實(shí)驗(yàn)者只需要按照說(shuō)明書(shū)上的步驟操作即可。使用時(shí)無(wú)需配制其它試劑，非常方便，適合于RNA的快速提取。所提取的RNA完整性好、純度高，可用于分子生物學(xué)后實(shí)驗(yàn)。但較好的試劑盒價(jià)格比較貴，在實(shí)驗(yàn)室可以根據(jù)自己的實(shí)驗(yàn)需要來(lái)定奪試劑盒的使用。 </p> <p> 一般包括：成分?jǐn)?shù)量?jī)?chǔ)藏溫度有效期裂解液100ml2-8℃一年漂洗液30mlRT一年洗柱液100mlRT一年RNasefreeddH2O30mlRT一年RNasefree吸附柱100個(gè)RT一年RNasefree收集管(2ml)100個(gè)RT一年此外，還配有一份試劑盒使用說(shuō)明書(shū)，根據(jù)不同的生產(chǎn)商，可能還配有不同的試劑，上海9分鐘完成RNA提取試劑盒500T/5865元，如：DNA酶、溶菌酶等。也是壓在國(guó)內(nèi)企業(yè)和患者身上的三座大山，上海9分鐘完成RNA提取試劑盒500T/5865元，導(dǎo)致國(guó)內(nèi)基因檢測(cè)企業(yè)運(yùn)轉(zhuǎn)效率低，上海9分鐘完成RNA提取試劑盒500T/5865元、盈利難、競(jìng)爭(zhēng)激烈。上海9分鐘完成RNA提取試劑盒500T/5865元</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海9分鐘完成RNA提取試劑盒500T/5865元,RNA提取試劑盒" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739414521485/5765636.png"></div><p> RNA提取注意事項(xiàng)（3） </p> <p> <br /> </p> <p> 破壁方法 </p> <p> 細(xì)胞或組織的徹底勻漿對(duì)RNA提取來(lái)說(shuō)，是一個(gè)很關(guān)鍵的步驟，它能夠防止RNA的損失和降解。勻漿的方法應(yīng)根據(jù)細(xì)胞或組織的類型來(lái)選擇。大部分培養(yǎng)的細(xì)胞可以置于細(xì)胞裂解液中，通過(guò)簡(jiǎn)單的渦旋震蕩來(lái)勻漿；而動(dòng)物組織、植物組織、酵母和細(xì)菌、真核細(xì)菌則常常需要更加劇烈的方法，通常用液氮研磨。比如說(shuō)細(xì)菌（特別是革蘭氏陽(yáng)性菌）的細(xì)胞壁，就需要溶菌酶消化來(lái)實(shí)現(xiàn)徹底的細(xì)胞裂解和RNA的比較大回收。酵母和革蘭氏陽(yáng)性菌通常還需要液氮研磨過(guò)程中加入玻璃微珠幫助破壁，酵母提取也可以加入諸如Lyticase等破壁酶幫助破壁，再用TRIpure或RNApure進(jìn)行提取。 </p> <p> <br /> 上海操作簡(jiǎn)單RNA提取試劑盒QQ 804036498將處理后的樣品在室溫放置5分鐘，使得核酸蛋白復(fù)合物完全分離。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海9分鐘完成RNA提取試劑盒500T/5865元,RNA提取試劑盒" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739419404297/3651782.png"></div><p> 意：此步驟目的是將純化柱中殘余的漂洗液去除，離心后將純化柱在室溫放置片刻，或置于超凈工作臺(tái)上通風(fēng)片刻，以充分晾干。如果有漂洗液殘留，可能會(huì)影響后續(xù)的RT等實(shí)驗(yàn)操作。 </p> <p> 將純化柱轉(zhuǎn)入一個(gè)新的離心管中，加30–100 μl RNase-free ddH2O，室溫放置2 min，4℃ 12,000 rpm (~13,400×g)離心2 min。 </p> <p> 洗脫緩沖液體積不應(yīng)少于30 μl，體積過(guò)小影響回收效率。且RNA應(yīng)保存在-70℃，以防降解。 </p> <p> 注意：如果想提高RNA得率，可重復(fù)上步操作一次，合并兩次得到的溶液。 </p><p> 緩慢加入0.5 倍體積無(wú)水乙醇，混勻(此時(shí)可能會(huì)出現(xiàn)沉淀)。將得到溶液和沉淀一起轉(zhuǎn)入純化柱中，4℃ 12,000 rpm (~13,400×g)離心30 s，若一次不能將全部溶液和混合物加入純化柱，請(qǐng)分兩次轉(zhuǎn)入純化柱中，4℃ 12,000 rpm (~13,400×g)離心30 s，棄掉收集管中的廢液。 </p> <p> 向純化柱中加入500 μl 溶液 RPI(使用前請(qǐng)先檢查是否已加入乙醇)，4℃ 12,000 rpm (~13,400×g)離心30 s，棄廢液。 </p> <p> 向純化柱中加入500 μl 溶液 RW(使用前請(qǐng)先檢查是否已加入乙醇)，室溫靜置2 min，4℃ 12,000 rpm (~13,400×g)離心30 s，棄廢液。 </p> <p> 向純化柱中加入500 μl 溶液 RW，室溫靜置2 分鐘，4℃ 12,000 rpm(~13,400×g)離心30 s，去除殘余液體。 </p> <p> 將純化柱入2ml 收集管中，4℃ 12,000 rpm (~13,400×g)離心2 min，去除殘余液體。芯片和試劑，對(duì)操作人員技術(shù)要求低，全程只需20分鐘，患者等待周期短，成本低。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海9分鐘完成RNA提取試劑盒500T/5865元,RNA提取試劑盒" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116738986650391/4176717.png"></div><p> RNA提取注意事項(xiàng)（1）: </p> <p> 迅速滅活內(nèi)源的RNA酶，以防止RNA降解。以下3個(gè)方法均可有效使內(nèi)源RNA酶失活： 1）用含離液（如胍鹽）的細(xì)胞裂解液收獲樣品，并立即勻漿。 2）用液氮瞬間凍結(jié)樣品。值得特別注意的是：組織塊必須保證足夠小，在浸入液氮的瞬間就能凍結(jié)，以確保瞬間令RNA酶失活。3）立即將樣品置于RNAfixer無(wú)液氮RNA樣品儲(chǔ)存液中。它是一種水相、的收集試劑，能立即穩(wěn)定并保護(hù)完整、未凍結(jié)的組織和細(xì)胞樣品中的RNA。關(guān)鍵要點(diǎn)是組織樣品切片一定要夠?。?0.5 cm），這樣RNAfixer才能在RNase破壞RNA之前迅速滲入組織塊中。但較好的試劑盒價(jià)格比較貴，在實(shí)驗(yàn)室可以根據(jù)自己的實(shí)驗(yàn)需要來(lái)定奪試劑盒的使用。上海操作簡(jiǎn)單RNA提取試劑盒QQ 804036498</p><p style="text-indent:25px">RNA在水溶液中OD值可能在1.5-1.9之間，但這并不表示RNA不純，需電泳檢測(cè)。上海9分鐘完成RNA提取試劑盒500T/5865元</p><p> 動(dòng)物組織：取新鮮或-70℃凍存100mg組織加1ml裂解液，用組織研磨杵或勻漿器勻漿處理。 </p> <p> 貼壁細(xì)胞：直接在培養(yǎng)板中加入裂解液裂解細(xì)胞，每106細(xì)胞加1ml 裂解液。用取樣器吹打混勻。 </p> <p> 細(xì)胞懸液：離心收集細(xì)胞。每106動(dòng)物、植物和酵母細(xì)胞或每107細(xì)菌細(xì)胞加1ml裂解液混勻。 </p> <p> 血液處理：取0.2-1ml新鮮血液加3倍體積紅細(xì)胞裂解液，混勻后室溫放置10分鐘，10000rpm離心1分鐘。棄上清，若沉淀含有紅細(xì)胞，可加入2倍體積紅細(xì)胞裂解液重復(fù)裂解步驟。離心后沉淀加入1 ml裂解液混勻。 </p> <p> 將處理后的樣品在室溫放置5分鐘，使得核酸蛋白復(fù)合物完全分離。上海9分鐘完成RNA提取試劑盒500T/5865元</p><p style="text-indent:25px">上海朝瑞生物科技有限公司創(chuàng)建于2003-01-22，注冊(cè)資金 700-1000萬(wàn)元，辦公設(shè)施齊全，辦公環(huán)境優(yōu)越，已全面實(shí)行網(wǎng)絡(luò)化辦公，大大提高了速度和效率。致力于創(chuàng)造高品質(zhì)的產(chǎn)品與服務(wù)，以誠(chéng)信、敬業(yè)、進(jìn)取為宗旨，以建zrbiorise,SPRICELL,scienceladde名牌產(chǎn)品為目標(biāo)，努力打造成為同行業(yè)中具有影響力的企業(yè)。公司不僅僅提供專業(yè)的1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長(zhǎng) 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗(yàn)檢測(cè)服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級(jí)改造產(chǎn)品服務(wù) 9.發(fā)布實(shí)驗(yàn)室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項(xiàng)目團(tuán)隊(duì)招聘13.發(fā)布研究生婚戀 14.發(fā)布項(xiàng)目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù),同時(shí)還建立了完善的售后服務(wù)體系,為客戶提供優(yōu)質(zhì)的產(chǎn)品和服務(wù)。上海朝瑞生物科技有限公司主營(yíng)業(yè)務(wù)涵蓋[ "科研技術(shù)服務(wù)", "應(yīng)用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學(xué)產(chǎn)品銷售" ]，堅(jiān)持“質(zhì)量第一、優(yōu)質(zhì)服務(wù)、顧客滿意”的質(zhì)量方針，贏得廣大客戶的支持和信賴。</p></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/273046.html</p></div></div></div></div></div><div id="kagekyu" class="page-right twoColRt"><div 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