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class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>北京七色免疫組化技術(shù)服務(wù),免疫組化</p><p><span>***更新：</span>2020-06-16 13:19:56</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="ib8dczb" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="p2elkbd" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="xlaw3ea" class="box"><p><p> 收藏查看我的收藏0有用+1已投票0免疫組織化學(xué)技術(shù)編輯鎖定免疫組織化學(xué)技術(shù)又稱為免疫細(xì)胞化學(xué)技術(shù)，是指用標(biāo)記的特異性抗原或抗體在組織細(xì)胞原位通過抗原抗體的免疫反應(yīng)和組織化學(xué)的呈色反應(yīng)，對(duì)相應(yīng)的抗原或抗體進(jìn)行定性、定位、定量測(cè)定的一項(xiàng)免疫學(xué)檢測(cè)方法。中文名免疫組織化學(xué)技術(shù)外文名Immunohistochemistrytechnique目錄1發(fā)展簡(jiǎn)史2現(xiàn)狀與展望免疫組織化學(xué)技術(shù)發(fā)展簡(jiǎn)史編輯免疫熒光組織化學(xué)是現(xiàn)***物學(xué)和醫(yī)學(xué)中廣泛應(yīng)用的技術(shù)之一，北京七色免疫組化技術(shù)服務(wù)，是由Coons和他的同事(1941)建立，免疫熒光技術(shù)與形態(tài)學(xué)技術(shù)相結(jié)合發(fā)展成免疫熒光細(xì)胞（或組織）化學(xué)。它與葡萄球菌A蛋白（SPA）、生物素與卵白素、植物血凝素（ConA等）相結(jié)合拓寬了領(lǐng)域；與激光技術(shù)、電子計(jì)算機(jī)，北京七色免疫組化技術(shù)服務(wù)，掃描電視和雙光子顯微鏡等技術(shù)結(jié)合發(fā)展為定量免疫熒光組織化學(xué)技術(shù)；熒光***細(xì)胞分類器Fluorescinactivatedcellsorter（FACS）的應(yīng)用，激光共聚焦顯微鏡的問世，北京七色免疫組化技術(shù)服務(wù)，使免疫熒光細(xì)胞技術(shù)發(fā)展到更高的階段，開創(chuàng)了免疫熒光技術(shù)的新領(lǐng)域。細(xì)胞顯微分光光度計(jì)與圖像分析儀的結(jié)合使免疫熒光組織化學(xué)的定量檢測(cè)更加準(zhǔn)確。北京七色免疫組化技術(shù)服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="北京七色免疫組化技術(shù)服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899748603516/652413.png"></div><p> 此方法尤其適合于內(nèi)源性生物素含量高的組織抗原檢測(cè)。4、幾種常用免疫組化方法簡(jiǎn)單介紹1）免疫熒光方法是**早建立的免疫組織化學(xué)技術(shù)。它利用抗原抗體特異性結(jié)合的原理，先將已知抗體標(biāo)上熒光素，以此作為探針檢查細(xì)胞或組織內(nèi)的相應(yīng)抗原，在熒光顯微鏡下觀察。當(dāng)抗原抗體復(fù)合物中的熒光素受激發(fā)光的照射后即會(huì)發(fā)出一定波長(zhǎng)的熒光，從而可確定組織中某種抗原的定位，進(jìn)而還可進(jìn)行定量分析。由于免疫熒光技術(shù)特異性強(qiáng)、靈敏度高、快速簡(jiǎn)便，所以在臨床病理診斷、檢驗(yàn)中應(yīng)用較廣。2）免疫酶標(biāo)方法免疫酶標(biāo)方法是繼免疫熒光后，于60年代發(fā)展起來(lái)的技術(shù)。基本原理是先以酶標(biāo)記的抗體與組織或細(xì)胞作用，然后加入酶的底物，生成有色的不溶性產(chǎn)物或具有一定電子密度的顆粒，通過光鏡或電鏡，對(duì)細(xì)胞表面和細(xì)胞內(nèi)的各種抗原成分進(jìn)行定位研究。免疫酶標(biāo)技術(shù)是**常用的技術(shù)。該方法與免疫熒光技術(shù)相比的主要優(yōu)點(diǎn)是：定位準(zhǔn)確，對(duì)比度好，染色標(biāo)本可長(zhǎng)期保存，適合于光、電鏡研究等。免疫酶標(biāo)方法的發(fā)展非常迅速，已經(jīng)衍生出了多種標(biāo)記方法，且隨著方法的不斷改進(jìn)和創(chuàng)新，其特異性和靈敏度都在不斷提高，使用也越來(lái)越方便。北京七色免疫組化技術(shù)服務(wù)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="北京七色免疫組化技術(shù)服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899748330079/709907.png"></div><p> 是一種小分子維生素，分子量為244，是轉(zhuǎn)氨甲?；^程中的輔酶。·抗生物素(avidin)，又稱卵白素或親和素，是一種分子量為67000的堿性蛋白，對(duì)生物素具有很強(qiáng)的親和力，比抗原抗體間的親和力要高出100萬(wàn)倍。它由4個(gè)亞基組成，每個(gè)亞基都有生物素的結(jié)合位點(diǎn)?！烧呔膳c抗體等大分子生物活性物質(zhì)相偶聯(lián)，又可被酶類等多種示蹤物所標(biāo)記，形成生物素-抗生物素系統(tǒng)。–該系統(tǒng)一端偶聯(lián)大分子生物反應(yīng)體系，另一端連結(jié)標(biāo)記物，后者加入酶的底物，產(chǎn)生顏色反應(yīng)。(1)標(biāo)記抗生物素—生物素法(labelledavidin-biotinmethod,LAB)：分為直接法和間接法。·直接法用生物素標(biāo)記***抗體，與抗原結(jié)合；酶標(biāo)記抗生物素，與生物素結(jié)合，然后進(jìn)行酶呈色反應(yīng)?！らg接法用生物素標(biāo)記二抗，酶標(biāo)記抗生物素，先用***抗體與組織抗原結(jié)合，再將第二抗體與***抗體相連結(jié)，***進(jìn)行呈色反應(yīng)。(2)橋抗生物素一生物素法(bridgeavidin-biotinmethod，BRAB)–此法是用生物素分別標(biāo)記抗體和酶，以抗生物素為橋，把二者連接起來(lái)，進(jìn)行呈色反應(yīng)。(3)抗生物素-生物素-過氧化物酶法(ABC法)·ABC法是在BRAB和LAB的基礎(chǔ)上改良的方法?！BC復(fù)合物是將過氧化物酶結(jié)合在生物素上。</p><p> 6、Indo-1Indo-1是典型的雙發(fā)射熒光探針，無(wú)鈣時(shí)在485nm左右有發(fā)射峰，結(jié)合鈣后，則在405nm處有發(fā)射峰，兩者的比值與細(xì)胞內(nèi)游離鈣離子濃度成線性關(guān)系，將此比值與標(biāo)準(zhǔn)曲線相比即可得出細(xì)胞內(nèi)游離鈣濃度，因此，可利用此探針定量檢測(cè)細(xì)胞內(nèi)游離鈣離子濃度。7、量子點(diǎn)(quantumdot)量子點(diǎn)是近年來(lái)研制的一種新型熒光染料，又稱半導(dǎo)體納米晶體，是由幾百或幾千個(gè)納米級(jí)顆粒構(gòu)成的半導(dǎo)體材料，性質(zhì)穩(wěn)定，溶于水，細(xì)胞本身不能合成和組裝。量子點(diǎn)具有熒光時(shí)間長(zhǎng)、產(chǎn)生多種顏色、檢測(cè)方便和應(yīng)用范圍廣等優(yōu)點(diǎn)。8、辣根過氧化物酶辣根過氧化物酶是一種糖蛋白，由于在辣根中該酶的含量很高，故得名。它以鐵卟啉為輔基，在過氧化氫存在時(shí)能催化苯酚、苯胺及其取代物聚合。通常用做光學(xué)和電子顯微鏡的組織化學(xué)示蹤物。該酶比活性高，穩(wěn)定，分子量小，純酶容易制備，所以較為常用，不但可以用于免疫組化染色，也***運(yùn)用于免疫類（ELISA）試劑盒。過氧化物酶作為多個(gè)試劑盒顯色體系的關(guān)鍵成分，對(duì)試劑盒的質(zhì)量有重要影響。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="北京七色免疫組化技術(shù)服務(wù),免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750107422/8042334.png"></div><p style="text-indent:25px">2. 免疫酶細(xì)胞化學(xué)技術(shù)是目前免疫組織化學(xué)研究中**常用的技術(shù)．基本原理是先以酶標(biāo)記的抗體與組織或細(xì)胞作用，然后加入酶的底物，生成有色的不溶性產(chǎn)物或具有一定電子密度的顆粒，通過光鏡或電鏡，對(duì)細(xì)胞或組織內(nèi)的相應(yīng)抗原進(jìn)行定位或定性研究．3. 免疫膠體金技術(shù)就是用膠體金標(biāo)記一抗，二抗或其他的能特異性的結(jié)合免疫球蛋白的分子（如葡萄球菌A蛋白）等作為探針對(duì)組織或細(xì)胞內(nèi)的抗原進(jìn)行定性，定位或定量研究．由于膠體金的電子密度高，多用于免疫電鏡的單標(biāo)記或多標(biāo)記的定位研究．天津多靶點(diǎn)免疫組化技術(shù)服務(wù)</p><p style="text-indent:25px">北京七色免疫組化技術(shù)服務(wù)</p><p> 免疫細(xì)胞化學(xué)技術(shù)組織印片·將潔凈載玻片輕壓于已暴露病灶的新鮮組織切面，細(xì)胞即粘附于玻片，晾干后浸入冷**或醋酸-乙醇固定10min，自然干燥后染片或-20℃保存。免疫細(xì)胞化學(xué)技術(shù)細(xì)胞培養(yǎng)片(細(xì)胞爬片)·貼壁細(xì)胞培養(yǎng)時(shí)，置蓋片于培養(yǎng)瓶中，使細(xì)胞在蓋片上生長(zhǎng)，達(dá)適當(dāng)密度后取出固定(**-20?C固定10～20min)，再進(jìn)行免疫染色。–蓋片的處理方法同載玻片的處理，但泡酸時(shí)間2h即可。–為了防止細(xì)胞脫片，可用多聚賴氨酸處理。免疫細(xì)胞化學(xué)技術(shù)細(xì)胞涂片·大多數(shù)細(xì)胞涂片由細(xì)胞懸液制成，包括：–血液、尿液、腦脊液；–體腔積液；–組織穿刺吸取，如骨髓、淋巴結(jié)或其他實(shí)質(zhì)性組織–懸浮培養(yǎng)的細(xì)胞或貼壁細(xì)胞經(jīng)消化后形成的懸液。免疫細(xì)胞化學(xué)技術(shù)細(xì)胞涂片(續(xù))·細(xì)胞涂片的方法：–手涂法·將細(xì)胞濃度調(diào)節(jié)到106/ml左右，可直接涂于載玻片上，但要均勻、不重疊?！D片范圍應(yīng)小于1cm直徑，以節(jié)約試劑。–涂片機(jī)涂片法·將細(xì)胞樣品制成2×105～6/ml細(xì)胞懸液，吸取50～100?l(1～2×104～5cells)加入涂片機(jī)內(nèi)，1000rpm離心2min后細(xì)胞就均勻分布于玻片上。免疫細(xì)胞化學(xué)技術(shù)常用染色方法根據(jù)標(biāo)記物的不同分為免疫熒光法、免疫酶標(biāo)法、親和組織化學(xué)法。北京七色免疫組化技術(shù)服務(wù)</p><p style="text-indent:25px">上海朝瑞生物科技有限公司創(chuàng)立于2003-01-22，總部位于上海市，是一家1.發(fā)布科研技術(shù) 2.發(fā)布應(yīng)用技術(shù) 3.發(fā)布專業(yè)專長(zhǎng) 4.發(fā)布科研成果 5.發(fā)布我的需求 6.發(fā)布篩選檢驗(yàn)檢測(cè)服務(wù) 7.發(fā)布協(xié)同代研發(fā)服務(wù) 8.發(fā)布升級(jí)改造產(chǎn)品服務(wù) 9.發(fā)布實(shí)驗(yàn)室及儀器設(shè)備共享 10.發(fā)布培訓(xùn)基地共享 11.發(fā)布工廠代加工及共享車間 12.發(fā)布科研/項(xiàng)目團(tuán)隊(duì)招聘13.發(fā)布研究生婚戀 14.發(fā)布項(xiàng)目整包服務(wù) 15.發(fā)布資源交換業(yè)務(wù)的公司。上海朝瑞科技深耕行業(yè)多年，始終以客戶的需求為向?qū)?，為客戶提?**的[ "科研技術(shù)服務(wù)", "應(yīng)用技術(shù)服務(wù)", "科研成果轉(zhuǎn)化", "科學(xué)產(chǎn)品銷售" ]。公司終堅(jiān)持自主研發(fā)創(chuàng)新發(fā)展理念，不斷優(yōu)化的技術(shù)、產(chǎn)品為客戶帶來(lái)效益，目前年?duì)I業(yè)額達(dá)到2000-3000萬(wàn)元。上海朝瑞科技創(chuàng)始人仲緒軍，始終關(guān)注客戶，以優(yōu)化創(chuàng)新的科技，竭誠(chéng)為客戶提供比較好的服務(wù)。</p></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/278521.html</p></div></div></div></div></div><div id="bebtxeq" class="page-right twoColRt"><div id="la8z7qn" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="rgdkmd3" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img 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