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class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>長沙病原微生物多樣性測序分析,病毒宏基因組測序</p><p><span>***更新：</span>2021-01-15 00:25:19</p><p><span id="click" >瀏覽次數(shù)：2次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="ym7xz3y" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海探普生物科技有限公司</a></p><p><span>聯(lián)系人：</span>喻利</p><p><span>郵箱: </span>380467011@qq.com</p><p><span>電話: </span>13166221237</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span>http://www.tpbio.com.cn</p><p><span>手機: </span>021-13166221</p><p><span>地址: </span>放鶴路1088號6號樓307</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="3nmjqsu" class="page-proshow02"><p class="title">詳細說明</p><div id="j7gc2ip" class="box"><p><p style="text-indent:25px">病毒是地球上數(shù)量多的生物實體，其中細菌病毒(即噬菌體)約有1031個類群，從海洋到陸地再到人體幾乎都是它們的棲息地。研究者將病毒視為調(diào)節(jié)人類生態(tài)系統(tǒng)的重要成員，人體內(nèi)主要包括真核病毒和噬菌體，包括雙鏈DNA(double-strandedDNA，長沙病原微生物多樣性測序分析,dsDNA)，單鏈DNA(single-strandedDNA,ssDNA)和RNA病毒。隨著對病毒研究的普遍開展，“病毒組”與“病毒組學(xué)”的概念也應(yīng)運而生，這些術(shù)語分別涵蓋了棲息在生態(tài)系統(tǒng)中的所有病毒及其基因組和對它們的研究(LefkowitzEJ,etal，長沙病原微生物多樣性測序分析，長沙病原微生物多樣性測序分析,2017)。根據(jù)病毒不同的特征進行分類，包括病毒的宿主范圍；病毒的形態(tài)學(xué)；病毒的基因組大?。徊《镜暮怂峤M成成分以及病毒的致病性。雖然所有的性狀在病毒分類學(xué)的確定中都很重要，但目前利用平均核苷酸同源性（ANI）和系統(tǒng)發(fā)育關(guān)系進行序列比較被視為定義和區(qū)分病毒群類的主要標準。宏基因組測序是在未知病原檢測中發(fā)揮了難以替代的作用。長沙病原微生物多樣性測序分析</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="長沙病原微生物多樣性測序分析,病毒宏基因組測序" src="https://tyunfile.71360.com/UploadFile/tpswkj/637444035971066778/7326012.png"></div><p style="text-indent:25px">病毒宏基因組學(xué)文庫中包含兆級的短序列片段（Reads）。通過不同的序列拼接軟件將Reads拼接較長的DNA序列片斷（Contigs）。數(shù)據(jù)組裝可通過K-mer分析評估各個樣品的測序深度，通過對SOAPdenovo設(shè)置不同K值，篩選較好組裝結(jié)果，對較好組裝結(jié)果進行校正，并統(tǒng)計Reads利用率。接著利用已測序生物體的DNA序列構(gòu)建數(shù)據(jù)庫，將拼接后的Contigs通過不同的鑒定方案，與數(shù)據(jù)庫里的DNA序列信息進行比對，確定該序列來自的生物群落，篩選有用的基因信息。此外，還可以用一些工具對序列進行基因預(yù)測（如Metagene、GeneMark、FragGeneScan等）。四川病原微生物多樣性測序分析找哪家通過對樣本進行宏病毒組測序，我們可以了解單個樣本里有什么病毒，相對含量是多少，優(yōu)勢物種是什么。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="長沙病原微生物多樣性測序分析,病毒宏基因組測序" src="https://tyunfile.71360.com/UploadFile/tpswkj/637444036025168597/2680991.png"></div><p style="text-indent:25px">宏基因組（也稱微生物環(huán)境基因組或元基因組）是1998年提出的新名詞，其定義為“thegenomesofthetotalmicrobiotafoundinnature”,即生境中全部微小生物遺傳物質(zhì)的總和。它包含了可培養(yǎng)的和未可培養(yǎng)的微生物的基因，目前主要指環(huán)境樣品中的細菌和的基因組總和。而所謂宏基因組學(xué)(或元基因組學(xué)，metagenomics)就是一種以環(huán)境樣品中的微生物群體基因組為研究對象，以功能基因篩選和/或測序分析為研究手段，以微生物多樣性、種群結(jié)構(gòu)、進化關(guān)系、功能活性、相互協(xié)作關(guān)系及與環(huán)境之間的關(guān)系為研究目的的新的微生物研究方法。一般包括從環(huán)境樣品中提取基因組DNA,進行高通量測序分析，或克隆DNA到合適的載體，導(dǎo)入宿主菌體，篩選目的轉(zhuǎn)化子等工作。</p><p style="text-indent:25px">病毒宏基因組學(xué)的應(yīng)用：病毒宏基因組學(xué)已經(jīng)應(yīng)用到人類、動物和環(huán)境中，涉及到農(nóng)業(yè)、工業(yè)及畜牧業(yè)等各個領(lǐng)域，其應(yīng)用范圍已延伸到海洋、湖水、熱泉、下水道等無機環(huán)境，以及組織病料、血液、呼吸道、動物排泄物等有機環(huán)境。應(yīng)用病毒宏基因組學(xué)的方法研究佛羅里達綠海龜?shù)睦w維狀瘤組織中發(fā)現(xiàn)了新型單鏈DNA病毒（Seaturtletornovirus1，STTV1）。分析了來自馬里蘭淡水湖中的RNA病毒宏基因組，結(jié)果獲得淡水湖中30多個RNA病毒家族序列，其中包含小RNA病毒、小雙股病毒及正黏病毒等。所謂宏基因組學(xué)(metagenomics)就是一種以環(huán)境樣品中的微生物群體基因組為研究對象。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="長沙病原微生物多樣性測序分析,病毒宏基因組測序" src="https://tyunfile.71360.com/UploadFile/tpswkj/637444036090032294/3752810.png"></div><p style="text-indent:25px">病原宏基因組測序（mNGS）可與實時熒光定量PCR（RT-PCR）技術(shù)聯(lián)合使用，實現(xiàn)優(yōu)勢互補?！癛T-PCR由于其檢測速度快、操作流程簡單、成本較低的原因，更適用于大規(guī)模的篩查中，以便快速獲得是否為核酸陽性的初步證據(jù)，而對于RT-PCR檢測陰性、但臨床表型高度疑似的患者，利用mNGS進一步確認可有效提高檢測結(jié)果的可靠性，并獲得是否有其他病原體傳染的信息?！睂τ赗T-PCR檢測陽性的樣本，也可以進一步利用mNGS進行病毒全序列的分析，從而幫助獲得病毒是否發(fā)生變異的信息，為疫苗、藥物研發(fā)等方面提供可靠證據(jù)。在探普生物進行病毒宏基因組測序的流程非常簡單。長沙病原微生物多樣性測序分析</p><p style="text-indent:25px">全國開設(shè)病毒宏基因組測序的公司不超過3家，只有探普生物是專門為病毒研究者服務(wù)的。長沙病原微生物多樣性測序分析</p><p style="text-indent:25px">探普生物對樣本進行宏病毒組測序?qū)嶒灮诙鷾y序技術(shù)。經(jīng)過核酸純化-文庫構(gòu)建-生物信息學(xué)分析這3大基本流程后，樣本轉(zhuǎn)換成了序列數(shù)據(jù)。先，在核酸純化環(huán)節(jié)，探普提供專門針對病毒的核酸純化樣本指南，以提高純度和得率，與此同時探普生物也提供核酸純化服務(wù)。第二，文庫構(gòu)建環(huán)節(jié)。樣本的核酸具備濃度低，總量少的特點。探普生物專門針對這一點開發(fā)了超微量核酸文庫構(gòu)建，可以將0.01ng/μl甚至更低濃度的核酸構(gòu)建成測序文庫。第三，生物信息學(xué)分析環(huán)節(jié)。寄生性質(zhì)的生物下機數(shù)據(jù)一般都伴隨大量的宿主和其他微生物的數(shù)據(jù)。探普生物基于該特點，優(yōu)化了自有數(shù)據(jù)庫，搭載了**的生物信息學(xué)分析流程，可處理復(fù)雜背景下的目標物種序列。長沙病原微生物多樣性測序分析</p><p style="text-indent:25px">上海探普生物科技有限公司是一家從事生物科技專業(yè)領(lǐng)域內(nèi)技術(shù)開發(fā)、技術(shù)轉(zhuǎn)讓、技術(shù)咨詢、技術(shù)服務(wù)，化工原料及產(chǎn)品（除危險化學(xué)品、監(jiān)控化學(xué)品、煙花爆竹、民用物品、易制毒化學(xué)品），實驗室設(shè)備，計算機、軟件及輔助設(shè)備，電子產(chǎn)品銷售，計算機軟件開發(fā)及維護，計算機信息系統(tǒng)集成服務(wù)等。的公司，是一家集研發(fā)、設(shè)計、生產(chǎn)和銷售為一體的專業(yè)化公司。上海探普生物深耕行業(yè)多年，始終以客戶的需求為向?qū)В瑸榭蛻籼峁?**的病毒測序，病毒全基因組測序，病毒宏基因組測序，未知病原鑒定。上海探普生物繼續(xù)堅定不移地走高質(zhì)量發(fā)展道路，既要實現(xiàn)基本面穩(wěn)定增長，又要聚焦關(guān)鍵領(lǐng)域，實現(xiàn)轉(zhuǎn)型再突破。上海探普生物創(chuàng)始人喻利，始終關(guān)注客戶，創(chuàng)新科技，竭誠為客戶提供良好的服務(wù)。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/2847051.html</p></div></div></div></div></div><div id="m8acj2m" class="page-right twoColRt"><div id="3yst78n" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="t3tvcoq" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" 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