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onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="aztxjli" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>河南宏病毒組測(cè)序檢測(cè),病毒宏基因組測(cè)序</p><p><span>***更新：</span>2021-01-24 04:16:41</p><p><span id="click" >瀏覽次數(shù)：1次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="3suo2jl" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海探普生物科技有限公司</a></p><p><span>聯(lián)系人：</span>喻利</p><p><span>郵箱: </span>380467011@qq.com</p><p><span>電話: </span>13166221237</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span>http://www.tpbio.com.cn</p><p><span>手機(jī): </span>021-13166221</p><p><span>地址: </span>放鶴路1088號(hào)6號(hào)樓307</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="z3jgskc" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="a3zlyvc" class="box"><p><p style="text-indent:25px">上海探普生物科技有限公司對(duì)樣本進(jìn)行宏病毒組測(cè)序?qū)嶒?yàn)是如何進(jìn)行的？探普生物對(duì)樣本進(jìn)行宏病毒組測(cè)序?qū)嶒?yàn)基于二代測(cè)序技術(shù)。經(jīng)過(guò)核酸純化-文庫(kù)構(gòu)建-生物信息學(xué)分析這3大基本流程后，樣本轉(zhuǎn)換成了序列數(shù)據(jù)。首先，在核酸純化環(huán)節(jié)，探普提供專門(mén)針對(duì)病毒的核酸純化樣本指南，以提高純度和得率，與此同時(shí)探普生物也提供核酸純化服務(wù)。第二，文庫(kù)構(gòu)建環(huán)節(jié)。樣本的核酸具備濃度低，總量少的特點(diǎn)。探普生物專門(mén)針對(duì)這一點(diǎn)開(kāi)發(fā)了超微量核酸文庫(kù)構(gòu)建，河南宏病毒組測(cè)序檢測(cè)，可以將0.01ng/μl甚至更低濃度的核酸構(gòu)建成測(cè)序文庫(kù)。第三，生物信息學(xué)分析環(huán)節(jié)，河南宏病毒組測(cè)序檢測(cè)。寄生性質(zhì)的生物下機(jī)數(shù)據(jù)一般都伴隨大量的宿主和其他微生物的數(shù)據(jù)。探普生物基于該特點(diǎn)，河南宏病毒組測(cè)序檢測(cè)，優(yōu)化了自有數(shù)據(jù)庫(kù)，搭載了**的生物信息學(xué)分析流程，可處理復(fù)雜背景下的目標(biāo)物種序列?？茖W(xué)家通過(guò)對(duì)病毒進(jìn)行全基因組測(cè)序，可以了解病毒不同傳播階段的變異情況。河南宏病毒組測(cè)序檢測(cè)</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="河南宏病毒組測(cè)序檢測(cè),病毒宏基因組測(cè)序" src="https://tyunfile.71360.com/UploadFile/tpswkj/637444036090032294/3752810.png"></div><p style="text-indent:25px">在探普生物進(jìn)行病毒宏基因組測(cè)序，樣品具備什么條件才可以獲得比較質(zhì)量的宏基因組分析效果？其他公司對(duì)用于測(cè)序的樣本的要求較高。而在探普生物進(jìn)行病毒宏基因組測(cè)序，基于探普的專有流程，樣本要求非常低，不要求總量到達(dá)微克，探普有專門(mén)的收樣標(biāo)準(zhǔn)和送樣流程。為了保證后續(xù)數(shù)據(jù)的有效利用率，需要客戶配合完成合格的取樣步驟和樣本前處理步驟。當(dāng)然探普也提供適當(dāng)?shù)臉颖咎幚矸?wù)。核酸性質(zhì)有RNA和DNA之分，核酸鏈還有單雙鏈之分，而核酸本質(zhì)不同和單雙鏈的不同，進(jìn)行同樣的實(shí)驗(yàn)步驟其效率也不一樣。這些特點(diǎn)決定了宏基因組測(cè)序是相對(duì)個(gè)性化定制化的一類服務(wù)，出于對(duì)結(jié)果真實(shí)性的尊重和保證，需要客戶和銷售人員充分溝通，設(shè)定合理的樣本處理方案。河南宏病毒組測(cè)序檢測(cè)對(duì)樣本進(jìn)行宏病毒組測(cè)序有什么意義？</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="河南宏病毒組測(cè)序檢測(cè),病毒宏基因組測(cè)序" src="https://tyunfile.71360.com/UploadFile/tpswkj/637444035692510323/329466.png"></div><p style="text-indent:25px">病毒宏基因組學(xué)文庫(kù)中包含兆級(jí)的短序列片段（Reads）。通過(guò)不同的序列拼接軟件將Reads拼接較長(zhǎng)的DNA序列片斷（Contigs）。數(shù)據(jù)組裝可通過(guò)K-mer分析評(píng)估各個(gè)樣品的測(cè)序深度，通過(guò)對(duì)SOAPdenovo設(shè)置不同K值，篩選較好組裝結(jié)果，對(duì)較好組裝結(jié)果進(jìn)行校正，并統(tǒng)計(jì)Reads利用率。接著利用已測(cè)序生物體的DNA序列構(gòu)建數(shù)據(jù)庫(kù)，將拼接后的Contigs通過(guò)不同的鑒定方案，與數(shù)據(jù)庫(kù)里的DNA序列信息進(jìn)行比對(duì)，確定該序列來(lái)自的生物群落，篩選有用的基因信息。此外，還可以用一些工具對(duì)序列進(jìn)行基因預(yù)測(cè)（如Metagene、GeneMark、FragGeneScan等）。</p><p style="text-indent:25px">基因測(cè)序技術(shù)在此次**中起到了至關(guān)重要的作用，我國(guó)科研單位在一個(gè)月之內(nèi)迅速發(fā)現(xiàn)了正確的致病病毒并完成測(cè)序，得到了WHO的贊許。我國(guó)科學(xué)研究者對(duì)病毒序列、變異、核酸檢測(cè)手段的分享也為全世界應(yīng)對(duì)COVID-19**帶來(lái)了極有力的支持。在病毒檢測(cè)過(guò)程中，一種測(cè)序技術(shù)獲得了市場(chǎng)的普遍關(guān)注，這個(gè)技術(shù)就是宏基因組測(cè)序（mNGS）技術(shù)。宏基因組學(xué)（Metagenomics），是以特定環(huán)境樣品中整個(gè)微生物群落基因組作為研究對(duì)象，無(wú)需分離培養(yǎng)，直接提取環(huán)境樣本的DNA進(jìn)行高通量測(cè)序。對(duì)于臨床而言，mNGS可以準(zhǔn)確的分析患者樣本全部微生物，這一點(diǎn)對(duì)于傳染性疾病的病原體研究具有極高的應(yīng)用價(jià)值。用二代測(cè)序?qū)颖具M(jìn)行宏病毒組測(cè)序，每一步都是很大的挑戰(zhàn)。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="河南宏病毒組測(cè)序檢測(cè),病毒宏基因組測(cè)序" src="https://tyunfile.71360.com/UploadFile/tpswkj/637444035971066778/7326012.png"></div><p style="text-indent:25px">近年來(lái)，新發(fā)人畜共患病呈現(xiàn)逐年遞增的趨勢(shì)，人類想要征服新發(fā)人畜共患病，就必須提前發(fā)掘潛在的新的致病的病原，并針對(duì)新病原進(jìn)行基因組分析、流行病學(xué)調(diào)查、致病機(jī)制及疫苗開(kāi)發(fā)等方面的研究。病毒宏基因組學(xué)是在宏基因組學(xué)基礎(chǔ)上發(fā)展起來(lái)研究特定環(huán)境中病毒的新興技術(shù)，該技術(shù)結(jié)合深度測(cè)序技術(shù)（第二代、第三代測(cè)序技術(shù)）已經(jīng)在人類、動(dòng)物、特定環(huán)境中挖掘出大量的新病毒，該技術(shù)不依賴于病毒培養(yǎng)及病毒序列，在國(guó)內(nèi)外被普遍應(yīng)用于新發(fā)人畜共患病病原的挖掘與臨床診斷。就病毒宏基因組學(xué)在動(dòng)物病毒研究中的應(yīng)用及研究進(jìn)展進(jìn)行了介紹。對(duì)樣本進(jìn)行宏病毒組測(cè)序于臨床，可以輔助臨床醫(yī)生進(jìn)行微生物侵染類疾病診斷并在一定程度提供用藥指導(dǎo)。河南宏病毒組測(cè)序檢測(cè)</p><p style="text-indent:25px">目前，構(gòu)建的病毒宏基因組學(xué)文庫(kù)主要有載體克隆文庫(kù)和基于高通量測(cè)序技術(shù)的加接頭的文庫(kù)。河南宏病毒組測(cè)序檢測(cè)</p><p style="text-indent:25px">病毒宏基因組測(cè)序又稱宏病毒組(Virome),是在宏基因組學(xué)理論的基礎(chǔ)上,結(jié)合現(xiàn)有的病毒分子生物學(xué)檢測(cè)技術(shù)而興起的一個(gè)新的學(xué)科分支。而所謂宏基因組學(xué)(metagenomics)就是一種以環(huán)境樣品中的微生物群體基因組為研究對(duì)象,以功能基因篩選和測(cè)序分析為研究手段,以微生物多樣性、種群結(jié)構(gòu)、進(jìn)化關(guān)系、功能活性、相互協(xié)作關(guān)系及與環(huán)境之間的關(guān)系為研究目的的新的微生物研究方法。一般包括從環(huán)境樣品中提取基因組DNA,克隆DNA到合適的載體,導(dǎo)入宿主菌體,篩選目的轉(zhuǎn)化子等工作。河南宏病毒組測(cè)序檢測(cè)</p><p style="text-indent:25px">上海探普生物科技有限公司致力于醫(yī)藥健康，是一家服務(wù)型的公司。公司業(yè)務(wù)分為病毒測(cè)序，病毒全基因組測(cè)序，病毒宏基因組測(cè)序，未知病原鑒定等，目前不斷進(jìn)行創(chuàng)新和服務(wù)改進(jìn)，為客戶提供良好的產(chǎn)品和服務(wù)。公司將不斷增強(qiáng)企業(yè)重點(diǎn)競(jìng)爭(zhēng)力，努力學(xué)習(xí)行業(yè)知識(shí)，遵守行業(yè)規(guī)范，植根于醫(yī)藥健康行業(yè)的發(fā)展。在社會(huì)各界的鼎力支持下，持續(xù)創(chuàng)新，不斷鑄造***服務(wù)體驗(yàn)，為客戶成功提供堅(jiān)實(shí)有力的支持。</p></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/2984117.html</p></div></div></div></div></div><div id="od8ug3c" class="page-right twoColRt"><div id="poyk7km" class="box01 margin10 clearfix"><h3><a 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