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href="http://www.cdcfah.com/cp/second-_nongye/">農(nóng)業(yè)</a>?<a href="http://www.cdcfah.com/cp/third-nongye_nlmyxmhz/">農(nóng)林牧漁項(xiàng)目合作</a>?上海多靶點(diǎn)免疫組化染色服務(wù) 歡迎來(lái)電上海朝瑞生物科技供應(yīng)</p><div id="7rlspry" class="leftbox clearfix"><h1>上海多靶點(diǎn)免疫組化染色服務(wù) 歡迎來(lái)電上海朝瑞生物科技供應(yīng)</h1><div id="laxuwdk" class="proBig"><div id="odfczwi" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899748330079/709907.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="fu38heb" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899748330079/709907.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="8l7dho7" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>上海多靶點(diǎn)免疫組化染色服務(wù)</p><p><span>***更新：</span>2020-06-18 08:23:27</p><p><span id="click" >瀏覽次數(shù)：1次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="8ynfcjq" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="7ipw3nk" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="ism728u" class="box"><p><p> 這樣你才能大膽地去**和糾正一些錯(cuò)誤的步驟，上海多靶點(diǎn)免疫組化染色服務(wù)，如抗體孵育條件主要是抗體濃度、溫度、時(shí)間，這三者一般是相互成反比的（相對(duì)），其中濃度是**重要的先決條件，溫度決定反應(yīng)的速度、時(shí)間決定反應(yīng)的量。比如溫度有4℃、室溫、37℃。我推薦4℃比較好，反應(yīng)**溫和，背景較淺；而37℃反應(yīng)速度較快，時(shí)間較短；室溫我不太提倡，除非你每次都把環(huán)境溫度控制在一定的范圍，否則，盡量選擇前兩者。2、定位和定性是免疫組化比較大的優(yōu)勢(shì)。相比于其他蛋白檢測(cè)方法，上海多靶點(diǎn)免疫組化染色服務(wù)，上海多靶點(diǎn)免疫組化染色服務(wù)，免疫組化具有定位較直接準(zhǔn)確、定性靈敏度高，是定位檢測(cè)分析優(yōu)先方法。尤其對(duì)于有些因子的轉(zhuǎn)位研究十分有用。3、免疫組化結(jié)果定量分析的前提是高質(zhì)量的染色切片。免疫組化結(jié)果也能定量分析，但必須是背景染色淺而特異性染色較深的情況下，分析**為準(zhǔn)確，這種原則可能也是我們?nèi)粘徃鍟r(shí)判定研究結(jié)果的必備條件。4、免疫組化實(shí)驗(yàn)一定要設(shè)置陽(yáng)性和陰性對(duì)照。陽(yáng)性對(duì)照一般是用肯定表達(dá)這種抗原的切片來(lái)做；陰性對(duì)照一般是用PBS或非一抗替代一抗來(lái)進(jìn)行反應(yīng)，其余步驟均一致。前者是排除方法和實(shí)驗(yàn)系統(tǒng)有無(wú)問(wèn)題；后者是排除有無(wú)一抗外的非特異性染色。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海多靶點(diǎn)免疫組化染色服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899748330079/709907.png"></div><p> 免疫組織化學(xué)是一項(xiàng)在醫(yī)學(xué)基礎(chǔ)與臨床科研中被廣泛應(yīng)用的一項(xiàng)技術(shù)，免疫組化染色服務(wù)也是生物公司的一項(xiàng)常規(guī)服務(wù)之一。該技術(shù)融合了抗原和抗體特異性結(jié)合的免疫學(xué)技術(shù)與組織學(xué)技術(shù)，通過(guò)化學(xué)反應(yīng)使標(biāo)記抗體的顯色劑顯色，來(lái)對(duì)組織或細(xì)胞內(nèi)抗原進(jìn)行定位和定量研究。肽類、***、神經(jīng)遞質(zhì)、細(xì)胞因子、受體、表面抗原等，它們的組織細(xì)胞內(nèi)具有抗原性的物質(zhì)，所以均可以用免疫組織化學(xué)方法顯示。免疫組化常用的顯色劑包括熒光素、酶、生物素和鐵蛋白等。11、金屬離子和金屬蛋白復(fù)合物 </p> <p> 金屬離子和金屬蛋白復(fù)合物如鐵蛋白、金和汞可以作為免疫組化反應(yīng)中的標(biāo)記物。如鐵蛋白等，主要應(yīng)用于免疫電鏡。免疫金銀法的基本原理是用特異性抗體與抗原反應(yīng)，隨后用金標(biāo)記的間接抗體或SPA蛋白，再與特異性抗體結(jié)合，用乳酸銀處理，使銀顆粒沉積在金顆粒上，還原銀反應(yīng)而顯示出黑褐色。適用于石蠟切片、冰凍切片，培養(yǎng)細(xì)胞，細(xì)胞涂片和樹(shù)脂包埋的電鏡切片。這種方法敏感性高，可檢測(cè)組織中微量抗原，定位準(zhǔn)確、無(wú)擴(kuò)散現(xiàn)象且背景清洗，對(duì)比度好、方法簡(jiǎn)便。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海多靶點(diǎn)免疫組化染色服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899748701172/8129573.png"></div><p> ）問(wèn)題及其解答：如何降低非特異性熒光染色和避免陰性著色？1、非特異性染色產(chǎn)生原因及其解決方案：⑴游離熒光素殘留在二抗中。一部分熒光素未與蛋白質(zhì)結(jié)合，形成了聚合物和衍化物，而不能被透析除去。二抗配置時(shí)用透析法或?qū)游龇ǚ蛛x熒光素標(biāo)記的二抗和游離的二抗。購(gòu)買高質(zhì)量、高純度的熒光素二抗。⑵抗體以外的血清蛋白與熒光素結(jié)合形成熒光素脲蛋白，可與組織成分結(jié)合。⑶組織抗原封閉不全。除去檢查的抗原以外，組織中還可能存在類屬抗原（如Forssman氏抗原），可與組織中特異性抗原以外之之相應(yīng)抗體結(jié)合。延長(zhǎng)血清封閉時(shí)間。⑷從組織中難于提純抗原性物質(zhì)，所以制備的免疫血清中往往混雜一些抗其他組織成分的抗體，以致容易混淆。⑸抗體分子上標(biāo)記的熒光素分子太多，這種過(guò)量標(biāo)記的抗體分子帶過(guò)多的陰離子，可吸附于正常組織上而呈現(xiàn)非特異性染色。⑹熒光素不純、標(biāo)本固定不當(dāng)?shù)?。⑺一抗和二抗孵育條件和濃度不合適。調(diào)整一抗和二抗孵育條件和濃度至比較好。⑻一抗和二抗孵育后的清洗不充分。增加清洗次數(shù)和延長(zhǎng)清洗時(shí)間。2、陰性染色產(chǎn)生的原因有：⑴一抗和二抗?jié)舛炔缓线m或孵育條件不妥。⑵熒光素提前衰退。</p></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/299342.html</p></div></div></div></div></div><div id="mrjgykh" class="page-right twoColRt"><div id="2dkce7e" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="ixpm8ia" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img 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