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onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="adnhoa7" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>上海四色免疫組化實(shí)驗(yàn)服務(wù)</p><p><span>***更新：</span>2020-06-18 15:41:02</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="2ik3ikh" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="jdkzbik" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="wldac8l" class="box"><p><p> 免疫組化細(xì)胞凋亡檢測細(xì)胞凋亡原理：正常凋亡細(xì)胞通過檢測凋亡細(xì)胞DNA片段進(jìn)行染色，正常的、人為造成凋亡的細(xì)胞很少能夠被染色。以甲基綠復(fù)染，便于從形態(tài)學(xué)上分辨評估正常細(xì)胞和凋亡細(xì)胞。首先確定目標(biāo)凋亡細(xì)胞的組織部位（如眼球組織，由于玻璃體結(jié)構(gòu)內(nèi)沒有細(xì)胞核，因此看不到凋亡，在整個組織的**外面有單層視網(wǎng)膜細(xì)胞，因此只能在**邊緣查看凋亡細(xì)胞的陽性產(chǎn)物）免疫組化關(guān)于掉片HE、Masson、番紅O、Nissl、Mallory等染色方法很少有掉片現(xiàn)象；免疫組化和細(xì)胞凋亡檢測我們采用專門購買的防脫片，由于整個實(shí)驗(yàn)流程比較長，在實(shí)驗(yàn)過程中產(chǎn)生掉片是很正常的現(xiàn)象。對于容易掉片的組織，如：骨組織、腦組織、皮膚組織等掉片有時候是不可避免的。免疫組化經(jīng)驗(yàn)總結(jié)編輯做過病理實(shí)驗(yàn)的人都知道，實(shí)驗(yàn)看似容易，上海四色免疫組化實(shí)驗(yàn)服務(wù)，但真想把它做好，還是需要花上很長一段時間去積累和摸索經(jīng)驗(yàn)。我從事病理實(shí)驗(yàn)已有6年多了，上海四色免疫組化實(shí)驗(yàn)服務(wù)，在這段時間里，我做過許許多多病理相關(guān)實(shí)驗(yàn)，上海四色免疫組化實(shí)驗(yàn)服務(wù)，剛開始啥也不懂，一味按照網(wǎng)上操作流程來做，但做的結(jié)果往往并不是十分理想，**終結(jié)果未能達(dá)到預(yù)期的效果。經(jīng)過一段時間的瀏覽和學(xué)習(xí)，從一些在論壇內(nèi)學(xué)習(xí)、請教，并結(jié)合實(shí)踐操作，我經(jīng)歷了初級——查找資料和摸索方法。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海四色免疫組化實(shí)驗(yàn)服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899751093750/8037763.png"></div><p> 6）滴加通用型IgG抗體-Fab段-HRP多聚體，室溫/37℃孵育30分鐘-1h，PBS/TBS沖洗，3分鐘×5次。7）應(yīng)用DAB溶液顯色。8）蒸餾水沖洗、復(fù)染、脫水、透明、封片。三、免疫熒光技術(shù)（略）1、酶免疫組化的關(guān)鍵環(huán)節(jié)1）標(biāo)本固定固定的目的是①防止標(biāo)本從玻片上脫落；②除去妨礙抗原-抗體結(jié)合的類脂，使抗原抗體結(jié)合物易于獲得良好的染色結(jié)果；③固定的標(biāo)本易于保存。固定劑的選擇一般用4%多聚甲醛，但睪丸組織、眼可能要選用Bouin’s液或mDF液效果較好。2）脫水、石蠟包埋和制片脫水用梯度乙醇（由低到高）充分脫水、對組織要完全浸蠟、切片時刀片要干凈和鋒利。否則，容易裂片和脫片等。3）脫蠟和水化這是為了后面的抗體等試劑能夠充分與組織中抗原等結(jié)合反應(yīng)。脫蠟可以先60℃20min，然后立即二甲苯1-3分別10min（這個時間是由二甲苯新鮮程度和室溫等綜合決定的），但當(dāng)天制好的切片一般先60℃3-4h。水化用梯度乙醇（由高到低）。若脫蠟和水化不全易出現(xiàn)局灶性反應(yīng)和浸洗不全，而產(chǎn)生非特異性背景著色。4）抗原修復(fù)由于組織中部分抗原在甲醛或多聚甲醛固定過程中，發(fā)生了蛋白之間交聯(lián)及醛基的封閉作用，從而失去抗原性；通過抗原修復(fù)，使得細(xì)胞內(nèi)抗原決定族重新暴露。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海四色免疫組化實(shí)驗(yàn)服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899748701172/8129573.png"></div><p> 在病理診斷中廣為使用的有ABC法、SP三步法、即用型二步法檢測系統(tǒng)等。3）免疫膠體金技術(shù)免疫膠體金技術(shù)是以膠體金這樣一種特殊的金屬顆粒作為標(biāo)記物。膠體金是指金的水溶膠，它能迅速而穩(wěn)定地吸附蛋白，對蛋白的生物學(xué)活性則沒有明顯的影響。因此，用膠體金標(biāo)記一抗、二抗或其他能特異性結(jié)合免疫球蛋白的分子（如葡萄球菌A蛋白)等作為探針，就能對組織或細(xì)胞內(nèi)的抗原進(jìn)行定性、定位，甚至定量研究。由于膠體金有不同大小的顆粒，且膠體金的電子密度高，所以免疫膠體金技術(shù)特別適合于免疫電鏡的單標(biāo)記或多標(biāo)記定位研究。由于膠體金本身呈淡至深紅色，因此也適合進(jìn)行光鏡觀察。如應(yīng)用銀加強(qiáng)的免疫金銀法則更便于光鏡觀察。5、被檢測的物質(zhì)組織或細(xì)胞中凡是能作為抗原或半抗原，如蛋白質(zhì)、多肽、氨基酸、多糖、磷脂、受體、酶、***、核酸及病原體等都可用相應(yīng)的特異性抗體進(jìn)行檢測。6、特點(diǎn)1）特異性強(qiáng)免疫學(xué)的基本原理決定抗原與抗體之間的結(jié)合具有高度特異性，因此，免疫組化從理論上講也是組織細(xì)胞中抗原的特定顯示，如角蛋白（keratin）顯示上皮成分，LCA顯示淋巴細(xì)胞成分。只有當(dāng)組織細(xì)胞中存在交叉抗原時，才會出現(xiàn)交叉反應(yīng)。2）敏感性高在應(yīng)用免疫組化的起始階段。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/303292.html</p></div></div></div></div></div><div id="bqnfmdf" class="page-right twoColRt"><div id="vpm2kmt" class="box01 margin10 clearfix"><h3><a 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