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href="http://www.cdcfah.com/cp/third-nongye_nlmyxmhz/">農(nóng)林牧漁項(xiàng)目合作</a>?上海多標(biāo)記免疫組化實(shí)驗(yàn)服務(wù) 歡迎來電上海朝瑞生物科技供應(yīng)</p><div id="sjfh2wa" class="leftbox clearfix"><h1>上海多標(biāo)記免疫組化實(shí)驗(yàn)服務(wù) 歡迎來電上海朝瑞生物科技供應(yīng)</h1><div id="ztvce87" class="proBig"><div id="ft2dk3t" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750869141/7134533.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="3gkbnmy" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750869141/7134533.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="2oqc8sp" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>上海多標(biāo)記免疫組化實(shí)驗(yàn)服務(wù)</p><p><span>***更新：</span>2020-06-19 05:22:29</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="xb82khe" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="yikrypb" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="jdkb7r3" class="box"><p><p> 免疫細(xì)胞化學(xué)技術(shù)組織印片·將潔凈載玻片輕壓于已暴露病灶的新鮮組織切面，細(xì)胞即粘附于玻片，晾干后浸入冷**或醋酸-乙醇固定10min，自然干燥后染片或-20℃保存。免疫細(xì)胞化學(xué)技術(shù)細(xì)胞培養(yǎng)片(細(xì)胞爬片)·貼壁細(xì)胞培養(yǎng)時(shí)，上海多標(biāo)記免疫組化實(shí)驗(yàn)服務(wù)，置蓋片于培養(yǎng)瓶中，使細(xì)胞在蓋片上生長，達(dá)適當(dāng)密度后取出固定(**-20?C固定10～20min)，再進(jìn)行免疫染色。–蓋片的處理方法同載玻片的處理，但泡酸時(shí)間2h即可。–為了防止細(xì)胞脫片，可用多聚賴氨酸處理。免疫細(xì)胞化學(xué)技術(shù)細(xì)胞涂片·大多數(shù)細(xì)胞涂片由細(xì)胞懸液制成，包括：–血液、尿液、腦脊液；–體腔積液；–組織穿刺吸取，如骨髓、淋巴結(jié)或其他實(shí)質(zhì)性組織–懸浮培養(yǎng)的細(xì)胞或貼壁細(xì)胞經(jīng)消化后形成的懸液。免疫細(xì)胞化學(xué)技術(shù)細(xì)胞涂片(續(xù))·細(xì)胞涂片的方法：–手涂法·將細(xì)胞濃度調(diào)節(jié)到106/ml左右，可直接涂于載玻片上，但要均勻、不重疊?！D片范圍應(yīng)小于1cm直徑，以節(jié)約試劑。–涂片機(jī)涂片法·將細(xì)胞樣品制成2×105～6/ml細(xì)胞懸液，吸取50～100?l(1～2×104～5cells)加入涂片機(jī)內(nèi)，1000rpm離心2min后細(xì)胞就均勻分布于玻片上，上海多標(biāo)記免疫組化實(shí)驗(yàn)服務(wù)，上海多標(biāo)記免疫組化實(shí)驗(yàn)服務(wù)。免疫細(xì)胞化學(xué)技術(shù)常用染色方法根據(jù)標(biāo)記物的不同分為免疫熒光法、免疫酶標(biāo)法、親和組織化學(xué)法。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海多標(biāo)記免疫組化實(shí)驗(yàn)服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750869141/7134533.png"></div><p> 多重?zé)晒饷庖呓M化與顯色法多重免疫組化不同，多重?zé)晒饷庖呓M化（mIHC）是基于酪胺信號放大（ Tyramide Signal Amplification，TSA）技術(shù)，可實(shí)現(xiàn)在同一組織切片樣本上對多個靶標(biāo)進(jìn)行區(qū)別標(biāo)記，進(jìn)而分析組織微環(huán)境中蘊(yùn)含的復(fù)雜信息。多靶標(biāo)組織原位的定位，半定量分析，數(shù)據(jù)更有價(jià)值高清共染圖片，助力高水平文章發(fā)表大幅提升低豐度蛋白的檢出幾率節(jié)省抗體用量，節(jié)約寶貴時(shí)間同一來源種屬抗體的多重標(biāo)記，無需更換經(jīng)典克隆節(jié)約珍貴樣本，小切片提供大信息。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海多標(biāo)記免疫組化實(shí)驗(yàn)服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750458985/5221034.png"></div><p> 我身邊有個研究生同學(xué)在我們實(shí)驗(yàn)室初次涉入免疫組化，聽說步驟不難，跟我后面做了幾次之后，就自己開始做了，連續(xù)做了三次，結(jié)果均是陰性染色。很掃興，不知是什么原因?qū)е碌?，后來我還是請教了上海舜田生物老師，她**提供給我我很大的幫助，解決問題的思路也逐步清晰。問題及其解答：免疫組化染色呈陰性結(jié)果的原因有哪些？1、抗體濃度和質(zhì)量問題以及抗體來源選擇錯誤。不知抗體是進(jìn)口的還是國產(chǎn)的工作液，怎么這么高稀釋度也沒能做出陽性結(jié)果？另外，不是抗體濃度越高就越易出現(xiàn)陽性結(jié)果，抗原抗體反應(yīng)有前帶和后帶效應(yīng)，必須摸索比較好濃度。2、抗原修復(fù)不全，對于甲醛固定的組織必須用充分抗原修復(fù)來打開抗原表位，以利于與抗體結(jié)合；建議微波修復(fù)用高火4次*6min試試。有人做過實(shí)驗(yàn)，這是比較好的時(shí)間和次數(shù)。若不行，還可高壓修復(fù)。3、組織切片本身這種抗原含量低；4、血清封閉時(shí)間過長。5、DAB孵育時(shí)間過短。6、細(xì)胞通透不全，抗體未能充分進(jìn)入胞內(nèi)參與反應(yīng)。7、開始做免疫組化，我建議你一定要首先做個陽性對照片，排除抗體等外的方法問題。我的實(shí)際解決方案：1、首先，排除組織切片內(nèi)的抗原有無丟失及其含量多少。免疫組化中兩個**重要的因素是抗原和抗體。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/309950.html</p></div></div></div></div></div><div id="2lx3qco" class="page-right twoColRt"><div id="7t8npbn" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="vfuwd8j" class="recomPic likePro"><a 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