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#f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>北京多色熒光免疫組化實(shí)驗(yàn)服務(wù)</p><p><span>***更新：</span>2020-06-19 05:22:29</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="bvh2g3j" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="xlnu2ah" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="ztvsm2v" class="box"><p><p> 是一種小分子維生素，分子量為244，是轉(zhuǎn)氨甲?；^程中的輔酶?！た股锼?avidin)，又稱卵白素或親和素，是一種分子量為67000的堿性蛋白，對生物素具有很強(qiáng)的親和力，比抗原抗體間的親和力要高出100萬倍。它由4個(gè)亞基組成，每個(gè)亞基都有生物素的結(jié)合位點(diǎn)?！烧呔膳c抗體等大分子生物活性物質(zhì)相偶聯(lián)，北京多色熒光免疫組化實(shí)驗(yàn)服務(wù)，又可被酶類等多種示蹤物所標(biāo)記，形成生物素-抗生物素系統(tǒng)。–該系統(tǒng)一端偶聯(lián)大分子生物反應(yīng)體系，另一端連結(jié)標(biāo)記物，后者加入酶的底物，產(chǎn)生顏色反應(yīng)，北京多色熒光免疫組化實(shí)驗(yàn)服務(wù)。(1)標(biāo)記抗生物素—生物素法(labelledavidin-biotinmethod,LAB)：分為直接法和間接法?！ぶ苯臃ㄓ蒙锼貥?biāo)記***抗體，與抗原結(jié)合；酶標(biāo)記抗生物素，與生物素結(jié)合，然后進(jìn)行酶呈色反應(yīng)?！らg接法用生物素標(biāo)記二抗，酶標(biāo)記抗生物素，先用***抗體與組織抗原結(jié)合，再將第二抗體與***抗體相連結(jié)，北京多色熒光免疫組化實(shí)驗(yàn)服務(wù)，***進(jìn)行呈色反應(yīng)。(2)橋抗生物素一生物素法(bridgeavidin-biotinmethod，BRAB)–此法是用生物素分別標(biāo)記抗體和酶，以抗生物素為橋，把二者連接起來，進(jìn)行呈色反應(yīng)。(3)抗生物素-生物素-過氧化物酶法(ABC法)·ABC法是在BRAB和LAB的基礎(chǔ)上改良的方法?！BC復(fù)合物是將過氧化物酶結(jié)合在生物素上。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="北京多色熒光免疫組化實(shí)驗(yàn)服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750849610/4353685.png"></div><p> 我一般初次做一種新抗體，喜歡先用說明書建議的低濃度和高濃度做一次，然后決定是向高還是低方向摸索。一般先決定二抗的濃度（工作液就好辦了，直接滴加），重點(diǎn)摸索一抗的**適濃度。注意：免疫反應(yīng)中存在前帶和后帶效應(yīng)，這提示不是濃度越高，陽性率就越高，反之亦然。⑷重要原因排除之后，也不要忽視抗體的質(zhì)量：原裝抗體一般比較穩(wěn)定，效果較好；而進(jìn)口分裝次之；工作液可能要注意質(zhì)量問題。3、同時(shí)，DAB的孵育時(shí)間可能要適當(dāng)延長，在鏡下觀察，有時(shí)可延長至30min。但一般3-10min比較好，此時(shí)背景也較淺。否則，說明抗體濃度不合適。4、***，血清封閉時(shí)間也可相應(yīng)縮短。一般10-30min，但這個(gè)時(shí)間可以調(diào)整，封閉主要是降低切片的總體背景著色。5、此外，細(xì)胞通透也不可忽視。許多戰(zhàn)友認(rèn)為石蠟切片一般不需細(xì)胞通透，因?yàn)榍衅瑫r(shí)可能已經(jīng)把細(xì)胞切開了，但對于胞**白，建議還是通透一下，促進(jìn)抗體等試劑充分進(jìn)入?yún)⑴c反應(yīng)。6、以上原因，都是針對實(shí)際中常見原因來進(jìn)行分析的，前提是排除操作者的操作錯(cuò)誤而引起陰性結(jié)果，這就需要新手還是要設(shè)置陽性對照以排除方法的問題。還有抗體稀釋液的pH值過低等其它原因的干擾?？傊氚衙庖呓M化做好，可能每一個(gè)環(huán)節(jié)都很重要。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="北京多色熒光免疫組化實(shí)驗(yàn)服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750869141/7134533.png"></div><p> 6、Indo-1Indo-1是典型的雙發(fā)射熒光探針，無鈣時(shí)在485nm左右有發(fā)射峰，結(jié)合鈣后，則在405nm處有發(fā)射峰，兩者的比值與細(xì)胞內(nèi)游離鈣離子濃度成線性關(guān)系，將此比值與標(biāo)準(zhǔn)曲線相比即可得出細(xì)胞內(nèi)游離鈣濃度，因此，可利用此探針定量檢測細(xì)胞內(nèi)游離鈣離子濃度。7、量子點(diǎn)(quantumdot)量子點(diǎn)是近年來研制的一種新型熒光染料，又稱半導(dǎo)體納米晶體，是由幾百或幾千個(gè)納米級顆粒構(gòu)成的半導(dǎo)體材料，性質(zhì)穩(wěn)定，溶于水，細(xì)胞本身不能合成和組裝。量子點(diǎn)具有熒光時(shí)間長、產(chǎn)生多種顏色、檢測方便和應(yīng)用范圍廣等優(yōu)點(diǎn)。8、辣根過氧化物酶辣根過氧化物酶是一種糖蛋白，由于在辣根中該酶的含量很高，故得名。它以鐵卟啉為輔基，在過氧化氫存在時(shí)能催化苯酚、苯胺及其取代物聚合。通常用做光學(xué)和電子顯微鏡的組織化學(xué)示蹤物。該酶比活性高，穩(wěn)定，分子量小，純酶容易制備，所以較為常用，不但可以用于免疫組化染色，也***運(yùn)用于免疫類（ELISA）試劑盒。過氧化物酶作為多個(gè)試劑盒顯色體系的關(guān)鍵成分，對試劑盒的質(zhì)量有重要影響。 </p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/309951.html</p></div></div></div></div></div><div id="2c28z4t" class="page-right twoColRt"><div id="srya87a" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="srdpjfh" 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