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clearfix tcPro"><section class="home-main container clearfix"><div id="xlht4tq" class="twoCol"><div id="qe2txsw" class="page-proshow clearfix"><p class="current"> 當前位置:<a href="http://www.cdcfah.com/">首頁?<a href="http://www.cdcfah.com/cp/">產(chǎn)品供應</a>?<a href="http://www.cdcfah.com/cp/second-_nongye/">農業(yè)</a>?<a href="http://www.cdcfah.com/cp/third-nongye_nlmyxmhz/">農林牧漁項目合作</a>?天津重組人源Claudin18.2蛋白***的選擇 **** 上海朝瑞生物科技供應</p><div id="u7g3lst" class="leftbox clearfix"><h1>天津重組人源Claudin18.2蛋白***的選擇 **** 上海朝瑞生物科技供應</h1><div id="x8w7evm" class="proBig"><div id="sr7ahyz" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637123969973388672/964933.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="wfwyxyp" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637123969973388672/964933.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="xfbyccj" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關鍵詞：</span>天津重組人源Claudin18.2蛋白***的選擇</p><p><span>***更新：</span>2020-05-25 05:34:06</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="cw8gizv" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機: </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="gov2amy" class="page-proshow02"><p class="title">詳細說明</p><div id="rfx337u" class="box"><p><p> 其序列的一致性為，該結果表明它們在進化上呈功能高度保守性。Claudin的分子質量為22-27kD。每一個Claudin分子均具有相同的結構，其肽鏈包含有4個跨膜區(qū)、2個細胞外環(huán)形結構和位于胞質中的羧基端、氨基端，天津重組人源Claudin18.2蛋白***的選擇。第l和第4個跨膜區(qū)以及第2個細胞外環(huán)的氨基酸序列具有高度保守性，天津重組人源Claudin18.2蛋白***的選擇。第4個跨膜區(qū)對閉鎖蛋白準確定位于緊密連接起重要的作用，若丟失將會導致閉鎖蛋白的羧基末端錯位到細胞外間隙。兩個細胞外環(huán)參與同種或異種Claudin蛋白之間的結合，對緊密連接條帶形成和離子通透選擇性具有重要作用。Claiidins***分布于正常組織和不同**組織，且存在差異性的表達。研究其在**發(fā)生、生長和轉移過程中的作用是近年的一個熱點。近年，在胃*前病變和胃*中也發(fā)現(xiàn)幾種claudins蛋白表達異常，并與預后有關?，F(xiàn)實生活中，8胃*、胰腺*、食道*以及轉移和未轉移卵巢*免疫***中存在著手術切除復發(fā)率高、化療和放療毒副作用強以及單抗***費用高的問題，迫切需要新的技術產(chǎn)生，而通過對claudins在胃*中異常分布和表達的研究，天津重組人源Claudin18.2蛋白***的選擇，人們能更好得理解胃*的發(fā)***展機制，從而有利于胃*的診斷和***。Claudins在胃*前病變、胃*各期和各型中的表達的研究是近年熱點。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津重組人源Claudin18.2蛋白***的選擇" src="http://tyunfile.71360.com/UploadFile/shchaorui/637123969973388672/964933.png"></div><p> 操作方法20包被先取96孔ELISA板，將(ug/ml),按100nL/孔加入板孔中，4t:包被過夜；倒掉包被液，加入封閉液，室溫下封存2h;棄去封閉液，用洗滌液洗6次，每次2min;分別加入倍比稀釋的山羊抗人(500ixg/ml)和抗血清，100pL/L，室溫下孵育lh;棄去抗體和抗血清，用洗漆液洗6次，每次3min;加入HRP標記的抗山羊二抗，100uL/孔，室溫孕育lh;棄去二抗，用洗滌液洗6次，每次3min;加入底物顯色液，100uL/L，室溫，顯色10-20min;加入終止液，50uL/孔，終止反應；酶標儀讀數(shù)，測定沒孔在490nm的吸光值。(3)免疫印跡反應測定血清抗分別取人胃*KATOin和胰腺*PANC-1細胞以及小鼠胃*MFC和胰腺*MPC-83細胞按照，結束后，按Bio-Rad產(chǎn)品說明，凝膠靠近陰極一側，硝酸纖維膜(NC膜)靠近陽極一側，置轉移緩沖液中(25mmol/LTris、192mmol/LGlycine、20%甲醇)，100V恒壓lh,將蛋白從凝膠電轉移至NC膜上，電轉移結束后，取出NC膜，用洗滌液TBST(含mol/LTBS、Tween20)室溫洗3次，浸入封閉液(含2%BSA的TBST)中37'C，1h，洗滌液(TOST)室溫洗3次，加荷瘤小鼠抗血清，37。C孵育1h，TBST室溫洗膜3次，加入兔抗山羊IgG-HRP二抗(中杉公司)，37。C孵育lh，TBST室溫洗膜3次，再用TBS洗3次。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津重組人源Claudin18.2蛋白***的選擇" src="http://tyunfile.71360.com/UploadFile/shchaorui/637123969946435547/5682602.png"></div><p style="text-indent:25px">上海朝瑞生物科技有限公司是一家成立于2003年的****，總部坐落于上海。公司自成立以來，秉承“真誠服務、合作雙贏”的經(jīng)營理念和良好的運作模式，現(xiàn)已發(fā)展成為產(chǎn)品種類豐富、技術力量雄厚、配套設施齊全的前列生物技術企業(yè)。我公司擁有良好的進貨渠道及國外采購合作伙伴，能夠為您提供全球數(shù)百家公司的數(shù)百萬種產(chǎn)品的現(xiàn)貨及期貨訂購服務（見列表）。同時，我公司竭誠為您提供以下生物工程技術服務：（1）美國Affymetrix系列基因芯片服務；（2）Illumina SNP平臺服務基因分型；（3）microRNA技術服務；（4）基因SNP課題研究服務；（5）生物信息學服務；（6）適時熒光定量（Realtime）PCR檢測服務；（7）RNA干擾服務；（8）全基因人工合成；（9）DNA合成服務；（10）多肽合</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津重組人源Claudin18.2蛋白***的選擇" src="http://tyunfile.71360.com/UploadFile/shchaorui/637123969950605469/9979953.png"></div></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/33072.html</p></div></div></div></div></div><div id="c8v9ara" class="page-right twoColRt"><div id="jipgkq8" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="tm3nzb2" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img 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