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clearfix"><p class="current"> 當前位置:<a href="http://www.cdcfah.com/">首頁?<a href="http://www.cdcfah.com/cp/">產(chǎn)品供應</a>?<a href="http://www.cdcfah.com/cp/second-_nongye/">農(nóng)業(yè)</a>?<a href="http://www.cdcfah.com/cp/third-nongye_nlmyxmhz/">農(nóng)林牧漁項目合作</a>?天津非編碼RNA 熒光定量PCR課題承包歡迎咨詢上海朝瑞生物科技供應</p><div id="oyqnznm" class="leftbox clearfix"><h1>天津非編碼RNA 熒光定量PCR課題承包歡迎咨詢上海朝瑞生物科技供應</h1><div id="lzb3jbt" class="proBig"><div id="2oe9oqx" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253049365234/1538935.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="lvsrd8g" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253049365234/1538935.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="tnpja9a" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關鍵詞：</span>天津非編碼RNA 熒光定量PCR課題承包</p><p><span>***更新：</span>2020-06-20 21:24:15</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="furtqu7" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機: </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="n2wd8ur" class="page-proshow02"><p class="title">詳細說明</p><div id="mgy7jl2" class="box"><p><p style="text-indent:25px">有關轉(zhuǎn)基因及其產(chǎn)品的安全性的爭論在國內(nèi)外愈演愈烈，各國**紛紛出臺一系列的政策、法規(guī)，天津非編碼RNA 熒光定量PCR課題承包，要求對轉(zhuǎn)基因作物進行標識，有些國家對轉(zhuǎn)基因的比較低含量做了限定，其閾值大小從1-5％不等。這就要求必須對轉(zhuǎn)基因及其產(chǎn)品進行檢測。常規(guī)的檢測方法多采用PCR法，如PCR-凝膠電泳、PCR-ELISA等。這些方法普遍存在著PCR產(chǎn)物污染，天津非編碼RNA 熒光定量PCR課題承包、特異性不高及速度慢等問題。熒光PCR技術是一種新近發(fā)展起來的檢測技術，它的應用將從根本上解決這些問題。 1.收集了國內(nèi)外商品化的轉(zhuǎn)基因作物品種，并通過查閱有關文獻及網(wǎng)站初步確定了各種作物的轉(zhuǎn)基因構建圖； 2．于反應體系中引入UNG去污染酶，建立了無污染熒光PCR擴增體系； 3．以植物內(nèi)源基因18SrRNA為靶標，設計了特異性引物和熒光探針，建立了以18SrRNA基因的擴增與否來判斷核酸提取質(zhì)量好壞的方法； 4．以FAM熒光素為標記，建立了以35S，天津非編碼RNA 熒光定量PCR課題承包、NOS、NPTⅡ、Pat、Bar、FMV和CryⅢa為篩選目標的轉(zhuǎn)基因農(nóng)作物熒光PCR定性檢測體系； 5．分別以FAM和TET兩種熒光素標記外源基因和內(nèi)源基因，建立了轉(zhuǎn)基因大豆及轉(zhuǎn)基因玉米的熒光定量PCR檢測體系。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津非編碼RNA 熒光定量PCR課題承包" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253049365234/1538935.png"></div><p> Ct（閾值循環(huán)）是擴增曲線與閾值線的交叉點（圖 1B）。它是 PCR 反應中靶標濃度的相對測量。除靶標濃度之外，還有很多因素會影響 Ct 的***值。我們將要討論最常見的可能影響 Ct 值的非模板依賴性因素，并描述如何評估實時熒光定量 PCR 反應的性能。 </p> <p> 顯示實時反應擴增圖的幾個參數(shù)。圖 1B 中的**期對應于圖 1C 中的線性期。閾值必須設在擴增圖的線性期中。Ct 值隨模板量減少而增加。然而，反應混合物或儀器中的任何干擾，只要能影響與 Ct 計算相關的熒光測定，都會使 Ct 值產(chǎn)生非模板依賴性的變化。因此，在不同條件下或用不同試劑進行的 PCR 反應產(chǎn)生的 Ct 值不可以直接進行比較。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津非編碼RNA 熒光定量PCR課題承包" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253046152344/6852880.png"></div><p style="text-indent:25px">一般而言，熒光擴增曲線可以分成三個階段：熒光背景信號階段、熒光信號**擴增階段和平臺期。在熒光背景信號階段，擴增的熒光信號被熒光背景信號所掩蓋，無法判斷產(chǎn)物量的變化;在平臺期，擴增產(chǎn)物已不再呈**級增加，PCR終產(chǎn)物量與起始模板量之間沒有線性關系，無法計算起始模板拷貝數(shù)。因此只有在熒光信號**擴增階段，PCR產(chǎn)物量的對數(shù)值與起始模板量之間存在線性關系，故選擇在這個階段進行定量分析。為了定量方便，在實時熒光定量 PCR 技術中引入了幾個重要的概念：擴增曲線、熒光閾值、CT值。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/330932.html</p></div></div></div></div></div><div id="sb8bqev" class="page-right twoColRt"><div id="icjbdkh" class="box01 margin10 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