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class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750869141/7134533.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="vvjr4id" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>江蘇五色免疫組化技術(shù)服務(wù)</p><p><span>***更新：</span>2020-06-21 05:21:03</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="vlvehw8" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="0sbbakt" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="7p95azm" class="box"><p><p> 多光譜成像技術(shù)多光譜成像技術(shù)，可俗稱免疫熒光多標(biāo)，即將我們常見的免疫熒光雙標(biāo)提高到六標(biāo)，實(shí)現(xiàn)了在同一張組織切片上同時標(biāo)記3~6種蛋白，解決了科研中經(jīng)常遇到的標(biāo)記蛋白種類太少，無法在同一張切片上觀察不同細(xì)胞或者多種蛋白分布情況的難題。那么多光譜成像技術(shù)是什么工作原理，同時又具有哪些更加強(qiáng)大的功能？ </p> <p> 多光譜成像技術(shù)由多標(biāo)染色、圖像采集和圖像分析三大模塊組成，江蘇五色免疫組化技術(shù)服務(wù)。多標(biāo)染色采用的是Opal/TSA多標(biāo)記免疫熒光技術(shù)，打破了常規(guī)免疫熒光技術(shù)的局限性，不受一抗的種屬來源限制，能夠在同一組織切片樣本上復(fù)染多達(dá)7種熒光標(biāo)記（含DAPI在內(nèi)）。圖像采集采用了Vectra全光譜圖像采集技術(shù)，江蘇五色免疫組化技術(shù)服務(wù)，江蘇五色免疫組化技術(shù)服務(wù)，這是***一代的組織切片成像分析系統(tǒng)，創(chuàng)新性的將光譜成像和切片全景掃描功能完美融合，實(shí)現(xiàn)了**性的技術(shù)跨越，通過光譜拆分能夠去除自發(fā)熒光的干擾，有效識別多重?zé)晒庑盘?，從而獲得***圖像，確保能夠進(jìn)行精細(xì)的定量定位分析。 </p> <p> <br /> </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇五色免疫組化技術(shù)服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750869141/7134533.png"></div><p> 5、免疫組化技術(shù)掌握與否的鑒定標(biāo)準(zhǔn)是同一切片或不同切片中不同抗原均從摸索濃度或條件而做出優(yōu)良的染色切片。我發(fā)現(xiàn)許多研究生把網(wǎng)上或者說明書摸索的反應(yīng)條件、濃度、方法步驟，重復(fù)運(yùn)用于同一性質(zhì)的切片和同一種抗體，做出來后就覺得自己已經(jīng)掌握了免疫組化方法，更換一種抗體后，居然連二抗的種屬來源都拿錯了。失敗往往促進(jìn)你去思考試驗(yàn)原理和過程，成功有時也讓自己很自傲。6、免疫組化的應(yīng)用***，是當(dāng)前實(shí)驗(yàn)研究的**重要方法之一。如今發(fā)SCI論文時，明顯感覺*靠量化的數(shù)據(jù)來發(fā)文章很難，加一些形態(tài)學(xué)數(shù)據(jù)或圖片，老外十分歡迎，可能是怕你學(xué)術(shù)造假吧。當(dāng)然也不能做假陽性或假陰性結(jié)果。7、實(shí)驗(yàn)方法需要動手和動腦。經(jīng)過了反復(fù)的動手和動腦，把理論原理運(yùn)用于實(shí)踐，在把實(shí)踐中發(fā)現(xiàn)的問題帶到理論知識中去解決，**終把理論與實(shí)踐融會貫通。必要時去各大論壇或者一些**公司尋求幫助，個人感覺上海舜田生物技術(shù)支持不錯，服務(wù)質(zhì)量也很好。下面先介紹下免疫組化的概念和常用方法，以便讓大家對免疫組化更深入地理解和掌握。概念和常用方法介紹1、定義用標(biāo)記的特異性抗體對組織切片或細(xì)胞標(biāo)本中某些化學(xué)成分的分布和含量進(jìn)行組織和細(xì)胞原位定性、定位或定量研究。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇五色免疫組化技術(shù)服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899750380860/9179133.png"></div><p> 在放入氨水中返蘭即可。13）封片為了長期保存，我們一般用中性樹膠等封片，避免產(chǎn)生氣泡，方法是直接在載玻片組織上滴一滴封片液，然后一手拿住蓋片某一拐角，而另一手拿對面的那個拐角，接近封片液近端的拐角先降低，直至接觸到液體時為止；當(dāng)發(fā)現(xiàn)液體接觸面在不斷彌散時，則可以緩慢降低另一拐角，這樣一般不會產(chǎn)生氣泡。2、免疫熒光方法中的重要環(huán)節(jié)1）冰凍切片制備建議用新鮮組織，否則組織細(xì)胞內(nèi)部結(jié)構(gòu)破壞，易使抗原彌散。選用干凈鋒利的刀片、組織一定要冷凍適度等，防止裂片和脫片嚴(yán)重。2）組織切片固定切好片風(fēng)干后立即用冰**等固定液進(jìn)行固定5-10min，尤其要較長時間保存的白片，一定要及時固定和適當(dāng)保存。3）血清封閉為防止內(nèi)源性非特異性蛋白抗原的結(jié)合，需要在一抗孵育前先用血清（與二抗來源一致）封閉，減弱背景著色。血清封閉的時間是可以調(diào)整的，一般10-30min。4）一抗孵育條件在免疫組化反應(yīng)中**重要，包括孵育時間和抗體濃度。一抗孵育溫度有幾種：4℃、室溫、37℃，其中4℃效果比較好；孵育時間：這與溫度、抗體濃度有關(guān)，一般37℃1-2h，而4℃過夜和從冰箱拿出后37℃復(fù)溫45min。具體條件還要摸索。5）二抗孵育條件二抗一般室溫或37℃30min-1h。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/334021.html</p></div></div></div></div></div><div id="6z2ueqh" class="page-right twoColRt"><div id="twl8nll" class="box01 margin10 clearfix"><h3><a 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