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tcPro"><section class="home-main container clearfix"><div id="utfr2sj" class="twoCol"><div id="dseqcjv" class="page-proshow clearfix"><p class="current"> 當(dāng)前位置:<a href="http://www.cdcfah.com/">首頁?<a href="http://www.cdcfah.com/cp/">產(chǎn)品供應(yīng)</a>?<a href="http://www.cdcfah.com/cp/second-_nongye/">農(nóng)業(yè)</a>?<a href="http://www.cdcfah.com/cp/third-nongye_nlmyxmhz/">農(nóng)林牧漁項目合作</a>?江蘇siRNA熒光定量PCR技術(shù)服務(wù) 歡迎咨詢上海朝瑞生物科技供應(yīng)</p><div id="duwypln" class="leftbox clearfix"><h1>江蘇siRNA熒光定量PCR技術(shù)服務(wù) 歡迎咨詢上海朝瑞生物科技供應(yīng)</h1><div id="8dur8t3" class="proBig"><div id="ra3qlxe" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253046611328/9318580.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="2vmdubd" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253046611328/9318580.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="eiu2t2d" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>江蘇siRNA熒光定量PCR技術(shù)服務(wù)</p><p><span>***更新：</span>2020-06-21 19:21:43</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="tnpb32g" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="dseg8zf" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="vpwn7kb" class="box"><p><p> 實時熒光定量PCR具有靈敏度高、特異性高和精確性高的優(yōu)點，此項技術(shù)已被應(yīng)用于分子生物學(xué)、醫(yī)學(xué)、食品檢測和環(huán)境監(jiān)測等多個領(lǐng)域。在分子生物學(xué)領(lǐng)域的研究應(yīng)用定量核酸濃度：傳統(tǒng)方法是用瓊脂糖凝膠電泳或者分光光度計測定核酸濃度，其結(jié)果不準(zhǔn)確而且易污染，實時熒光定量PCR可以解決這些問題，它的準(zhǔn)確性、靈敏度高且無污染，對一些傳染性疾病進(jìn)行定量分析、病原微生物和***含量檢測，江蘇siRNA熒光定量PCR技術(shù)服務(wù)，此項技術(shù)都是優(yōu)先 </p> <p> 研究基因表達(dá)：應(yīng)用實時定量PCR技術(shù)可以對基因時間、空間表達(dá)水平差異進(jìn)行比較。例如，江蘇siRNA熒光定量PCR技術(shù)服務(wù)，江蘇siRNA熒光定量PCR技術(shù)服務(wù)，對特定基因用物理、化學(xué)、***等不同方法處理后的差異進(jìn)行比較，為人們的科學(xué)研究提供依據(jù) </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇siRNA熒光定量PCR技術(shù)服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253046611328/9318580.png"></div><p> RNA清洗移去上清液，每1mlTRIZOL試劑裂解的樣品中加入至少1ml的75%乙醇(75%乙醇用DEPCH2O配制)，清洗RNA沉淀?；靹蚝?，4℃下7000rpm離心5分鐘。 </p> <p> RNA干燥小心吸去大部分乙醇溶液，使RNA沉淀在室溫空氣中干燥5-10分鐘。 </p> <p> 溶解RNA沉淀溶解RNA時，先加入無RNA酶的水40μl用***反復(fù)吹打幾次，使其完全溶解，獲得的RNA溶液保存于-80℃待用。 1)紫外吸收法測定 </p> <p> 先用稀釋用的TE溶液將分光光度計調(diào)零。然后取少量RNA溶液用TE稀釋(1:100)后，讀取其在分光光度計260nm和280nm處的吸收值，測定RNA溶液濃度和純度。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇siRNA熒光定量PCR技術(shù)服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253049365234/1538935.png"></div><p> ***定量：用已知的標(biāo)準(zhǔn)曲線對未知樣本的量進(jìn)行推算的方法為***定量法。此種方法需要明確標(biāo)準(zhǔn)樣品的濃度情況，然后稀釋標(biāo)準(zhǔn)樣品，分為幾個不同梯度濃度然后進(jìn)行反應(yīng)測試。橫坐標(biāo)為標(biāo)準(zhǔn)品拷貝數(shù)的對數(shù)值，縱坐標(biāo)為測得的Ct值，進(jìn)而繪制出標(biāo)準(zhǔn)曲線。以標(biāo)準(zhǔn)曲線為基礎(chǔ)，在定量分析未知樣品過程中根據(jù)其ct值能夠?qū)悠鹗伎截悢?shù)推算出 </p> <p> 實時熒光定量PCR技術(shù)總結(jié)為以下幾點。特異性強(qiáng) 靈敏度高重復(fù)性好檢測速度快自動化程度高 </p> <p> 實時熒光定量PCR具有靈敏度高、特異性高和精確性高的優(yōu)點，此項技術(shù)已被應(yīng)用于分子生物學(xué)、醫(yī)學(xué)、食品檢測和環(huán)境監(jiān)測等多個領(lǐng)域 </p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/341650.html</p></div></div></div></div></div><div id="abtk7mo" 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