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id="rz2fbpf" class="proBig"><div id="2s32eul" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899751181641/619319.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="mpgctkl" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899751181641/619319.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="y3maqw3" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>北京多色熒光免疫組化</p><p><span>***更新：</span>2020-06-25 04:25:30</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="btuv38r" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話(huà): </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線(xiàn)] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="2tcxyea" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="hk8rhso" class="box"><p><p> 使用的玻片等載體，都必須厚度均勻，北京多色熒光免疫組化，無(wú)明顯的自發(fā)熒光，如果使用油鏡，北京多色熒光免疫組化，還必須保證鏡油為無(wú)熒光鏡油；電源比較好裝穩(wěn)壓器，否則電壓不穩(wěn)不僅會(huì)降低汞燈的壽命，也會(huì)影響鏡檢的效果。3、免疫組化和免疫熒光結(jié)果分析：見(jiàn)***場(chǎng)試驗(yàn)講座。案例一DAB染色后切片著色一片黃/背景深背景：奧運(yùn)會(huì)期間我們課題組研究生都在實(shí)驗(yàn)室做實(shí)驗(yàn)，許多從北京購(gòu)買(mǎi)的試劑買(mǎi)不到，對(duì)我們的許多實(shí)驗(yàn)產(chǎn)生了很大的影響。我以前一直用中杉金橋的SP三步法染色試劑盒，效果不錯(cuò)，在奧運(yùn)會(huì)期間我準(zhǔn)備做同批實(shí)驗(yàn)分不同批次酶免疫組化實(shí)驗(yàn)，***批組化實(shí)驗(yàn)結(jié)果很好，抗體濃度感覺(jué)比較合適（SantaCruz公司1：200），等做第二批時(shí)發(fā)現(xiàn)封閉血清不夠了，為了保證實(shí)驗(yàn)順利進(jìn)行，我又從福州邁新頂了一個(gè)SP試劑盒，同時(shí)抗體稀釋液也不夠了，我又重新從博士德公司買(mǎi)了一小瓶10ml。從第二批實(shí)驗(yàn)開(kāi)始，組化實(shí)驗(yàn)結(jié)果一直顯示強(qiáng)背景著色，特異性染色很難辨別。后來(lái)我將標(biāo)本拿到上海舜田生物去做，北京多色熒光免疫組化，做了效果很不錯(cuò)，于是我就請(qǐng)教了他們公司從事病理30多年經(jīng)驗(yàn)的老師，她告訴我應(yīng)該如何去分析和解決問(wèn)題，很快我就找到了思路，結(jié)果問(wèn)題終于解決了。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="北京多色熒光免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899751181641/619319.png"></div><p> 9、堿性磷酸酶堿性磷酸酶（AKP），又稱(chēng)堿性磷酸單酯酶，是酶組織化學(xué)中**常用的酶之一。分布***，常見(jiàn)于活躍運(yùn)輸?shù)哪ど?。該酶在堿性環(huán)境下能催化各種醇和酚的磷酸酯水解，**適pH為，可被Mg2+、Mn2+和某些氨基酸所***；**物、碘液、砷酸鹽等可***其活性。顯示AKP的方法很多，主要有鈣鈷法和同時(shí)偶聯(lián)法。前者所需試劑便宜，容易購(gòu)買(mǎi)，操作簡(jiǎn)單，但會(huì)出現(xiàn)假陽(yáng)性。后者方法較靈敏，而且在正常組織內(nèi)不存在萘酚，因此不會(huì)出現(xiàn)假陽(yáng)性，但所需試劑較難購(gòu)買(mǎi)。10、生物素（biotin）生物素是B族維生素之一。生物素在羧化、脫羧和轉(zhuǎn)羧化反應(yīng)中起輔酶作用。生物素是含硫水溶性維生素。廣布于動(dòng)物及植物組織，已從肝提取物和蛋黃中分離，是多種羧化酶輔基的成分。生物素標(biāo)記反應(yīng)簡(jiǎn)單、溫和且很少***抗體活性，生物素標(biāo)記的抗體可存放多年而不失活。能與生物素結(jié)合的二級(jí)試劑素，所需的各種標(biāo)記物均可購(gòu)置。此外，親和素和鏈霉親和素的結(jié)合背景水平非常低，結(jié)合緊密且快速，抗體很容易與經(jīng)過(guò)化學(xué)修飾的活化生物素結(jié)合。生物素又可以與結(jié)合有酶、熒光染料的親和素或鏈霉親和素連接，后者已有商品試劑供應(yīng)。這樣只需將純化抗體與生物素結(jié)合，就可用各種不同的標(biāo)記物來(lái)檢測(cè)。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="北京多色熒光免疫組化" src="http://tyunfile.71360.com/UploadFile/shchaorui/637124899748603516/652413.png"></div><p> 免疫細(xì)胞化學(xué)技術(shù)免疫熒光法【原理】·用于免疫熒光的標(biāo)記物是小分子的熒光素，可標(biāo)記抗體或抗原；免疫熒光技術(shù)·熒光素經(jīng)某種特定波長(zhǎng)的光照射激發(fā)后，能發(fā)射出一種比激發(fā)光波波長(zhǎng)更長(zhǎng)而且能量較低的熒光，籍此可作定位觀察或示蹤；·借助于熒光顯微鏡進(jìn)行觀察。1、常用的熒光素·(1)異硫氰酸熒光素(FluoresceinIsothiocyanate,FITC)·(2)四甲基異硫氰酸羅丹明(TetramethylRhodamineIsothiocyanate,TRITC)·(3)四乙基羅丹明(RB200)·(4)碘化丙啶(propidiumiodide,PI)(1)異硫氰酸熒光素(FITC)·易溶于水和乙醇?！け容^大吸收光譜為490～495nm，比較大發(fā)射光譜為520～530nm．呈翠綠色熒光，分子量。·在堿性條件下，F(xiàn)ITC的異硫氰酸基在水溶液中與Ig的自由氨基形成共價(jià)鍵，成為標(biāo)記的熒光抗體。一個(gè)lgG分子上**多能標(biāo)記15～20個(gè)FITC分子。(2)四甲基異硫氰酸羅丹明(TRITC)·比較大吸收光譜550nm，比較大發(fā)射光譜620nm，呈紅色熒光，分子量為444?！づc蛋白質(zhì)結(jié)合的方式同F(xiàn)ITC。(3)四乙基羅丹明(RB200)·不溶于水，易溶于乙醇和**?！け容^大吸收光譜為570nm，比較大發(fā)射光譜為595～600nm，呈橙紅色熒光，分子量為580。·RB200在五氯化磷(PCl5)作用下轉(zhuǎn)變成磺酰氯(SO2Cl)。</p></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/382291.html</p></div></div></div></div></div><div id="wo8d7lc" class="page-right twoColRt"><div 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