北京新手RNA提取試劑盒50T/690元 **** 上海朝瑞生物科技供應(yīng)-b2b網(wǎng)站_b2b電子商務(wù)平臺(tái)【買產(chǎn)品】-上?？谄蚴称酚邢薰?/title><meta name="description" content=".樣品處理a，北京新手RNA提取試劑盒50T/690元.植物組織：取新鮮或-70℃凍存100mg組織在液氮中研磨，把粉末加入到1ml裂解液中混勻。b.動(dòng)物組織：取新鮮或-70℃凍存100mg組織加1ml裂解液，用組織研磨杵或勻漿器勻漿處理。"><meta name="keywords" content="北京新手RNA提取試劑盒50T/690元"><link rel="canonical" href="http://www.cdcfah.com/"/><meta name="applicable-device" content="pc"><link rel="shortcut icon" href="/static/new_net_01/images/favicon.ico"><link rel="stylesheet" href="/static/new_net_01/css/slick.css" /><link rel="stylesheet" href="/static/new_net_01/css/addnew.css"><link rel="stylesheet" href="/static/new_net_01/css/sidenav.css"><link rel="stylesheet" href="/static/new_net_01/css/style.css" /><link rel="stylesheet" href="/static/new_net_01/css/tab.css"><link rel="stylesheet" href="/static/new_net_01/css/404.css"> <meta name="baidu-site-verification" content="OBVzrC9aaQ" /> <meta http-equiv="Cache-Control" content="no-transform" /> <meta 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class="proBig"><div id="tnzbnkw" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739414501953/9962850.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="feln2x3" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739414501953/9962850.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="m229rac" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>北京新手RNA提取試劑盒50T/690元</p><p><span>***更新：</span>2020-06-25 05:22:09</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="8g2lvxe" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="pbd2n97" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="imt224z" class="box"><p><p style="text-indent:25px">. 樣品處理a，北京新手RNA提取試劑盒50T/690元. 植物組織：取新鮮或-70℃凍存100mg組織在液氮中研磨，把粉末加入到1ml裂解液中混勻。b. 動(dòng)物組織：取新鮮或-70℃凍存100mg組織加1ml裂解液，用組織研磨杵或勻漿器勻漿處理。c. 貼壁細(xì)胞：直接在培養(yǎng)板中加入裂解液裂解細(xì)胞，北京新手RNA提取試劑盒50T/690元，每106細(xì)胞加1ml 裂解液。用取樣器吹打混勻。d. 細(xì)胞懸液：離心收集細(xì)胞。每106動(dòng)物、植物和酵母細(xì)胞或每107細(xì)菌細(xì)胞加1ml裂解液混勻。e. 血液處理：取0.2-1ml新鮮血液加3倍體積紅細(xì)胞裂解液，北京新手RNA提取試劑盒50T/690元，混勻后室溫放置10分鐘， 10000rpm離心1分鐘。棄上清，若沉淀含有紅細(xì)胞，可加入2倍體積紅細(xì)胞裂解液重復(fù)裂解步驟。離心后沉淀加入1 ml裂解液混勻。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="北京新手RNA提取試劑盒50T/690元" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739414501953/9962850.png"></div><p> 樣品勻漿后，加入氯仿前，可在-80°C 凍存放置一個(gè)月。 </p> <p> RNA 沉淀可以保存在 75%乙醇中，2-8°C 放置一周或-20°C 放置一年。 </p> <p> 該試劑盒***于專業(yè)人員的科學(xué)研究使用，不得用于臨床診斷或***，更不得用于食品或藥品中。 </p> <p> 實(shí)驗(yàn)步驟、細(xì)胞裂解或組織勻漿： </p> <p> a貼壁細(xì)胞：吸盡培液，按照比例加入 Trizol 消化細(xì)胞，即每 10 平方厘米細(xì)胞中加入 1 ml Trizol，六孔板每孔加 1 ml Trizol，12 孔板每孔加 0.5 ml Trizol?；蝿?dòng) 3-5 下，再用移液器反復(fù)吹打幾下，確保細(xì)胞全部裂解，然后將溶液轉(zhuǎn)移至離心管中?！咀ⅰ考尤?Trizol 量不足時(shí)，會(huì)導(dǎo)致 DNA 污染。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="北京新手RNA提取試劑盒50T/690元" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116739429267578/3679475.png"></div><p> RNA 完整性檢測(cè) </p> <p> 取 1 μl RNA 加入適當(dāng) RNA loading buffer，混勻，進(jìn)行電泳檢測(cè)。若出現(xiàn)清晰的三條帶，分別為 28S、18S 和 5S，且條帶之間界限清晰，就證明 RNA 樣品的完整性非常好?？蓞⒖家韵聢D示： </p> <p> RNA 純度檢測(cè) </p> <p> 利用分光光度計(jì)或者NanoDrop 儀檢測(cè)RNA 樣品在260nm、280nm 處的OD 值，并計(jì)算 A260/A280 的比值。純的 RNA 的比值應(yīng)在 2.0 左右。 </p> <p> <br /> </p> <p> 提取到的 RNA 得率很低?？赡艿脑蛉缦拢?</p> <p> 樣品裂解或勻漿處理不徹底。 </p> <p> RNA 沉淀?xiàng)l件和時(shí)間太短，沒有充分沉淀 RNA 造成 RNA 損失。 </p> <p> ***得到的 RNA 沉淀未完全溶解。 </p> <p> <br /> </p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/382800.html</p></div></div></div></div></div><div id="qfhofr3" class="page-right twoColRt"><div id="ktk2aul" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="la2gnug" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" alt="福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></div><p>福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)</p></a></div><div id="oszw248" class="recomPic likePro"><a 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