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#f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>天津DNA甲基化熒光定量PCR實(shí)驗(yàn)技術(shù)服務(wù)</p><p><span>***更新：</span>2020-06-25 14:52:13</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="c23skhe" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="84zg7cj" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="tnpwlcz" class="box"><p><p style="text-indent:25px">SYBR Green I法：SYBR Green I是一種具有綠色激發(fā)波長(zhǎng)的染料，比較大吸收波長(zhǎng)約為 497 nm，發(fā)射波長(zhǎng)比較大約為 520 nm，可以和所有的 dsDNA 雙螺旋小溝區(qū)域結(jié)合。因?yàn)樵谟坞x狀態(tài)下 SYBR Green I 發(fā)出的熒光較弱，但是當(dāng)它與雙鏈 DNA 結(jié)合后，天津DNA甲基化熒光定量PCR實(shí)驗(yàn)技術(shù)服務(wù)，熒光就會(huì)**增強(qiáng)，而且熒光信號(hào)的增加與 PCR 產(chǎn)物的增加完全同步，天津DNA甲基化熒光定量PCR實(shí)驗(yàn)技術(shù)服務(wù)。此法的優(yōu)點(diǎn)是它可以監(jiān)測(cè)任何 dsDNA 序列的擴(kuò)增，檢測(cè)方法較為簡(jiǎn)單，成本較低，天津DNA甲基化熒光定量PCR實(shí)驗(yàn)技術(shù)服務(wù)，但也正是由于熒光染料能和任何dsDNA 結(jié)合，如非特異性擴(kuò)增產(chǎn)物和引物二聚體也能與染料結(jié)合而產(chǎn)生熒光信號(hào)，使實(shí)驗(yàn)產(chǎn)生假陽(yáng)性結(jié)果，因此其特異性不如探針?lè)?。因?yàn)榉翘禺愋援a(chǎn)物和引物二聚體的變性溫度要比目標(biāo)產(chǎn)物的低，所以可以在熔解曲線反應(yīng)過(guò)程中利用軟件分析儀器收集到信號(hào)進(jìn)行鑒別</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津DNA甲基化熒光定量PCR實(shí)驗(yàn)技術(shù)服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253046152344/6852880.png"></div><p> 轉(zhuǎn)基因：利用熒光定量PCR技術(shù)將轉(zhuǎn)基因信號(hào)擴(kuò)增放大。 </p> <p> 在環(huán)境監(jiān)測(cè)方面的應(yīng)用實(shí)時(shí)熒光定量PCR技術(shù)已經(jīng)被國(guó)內(nèi)外應(yīng)用在環(huán)境監(jiān)測(cè)中，提高了監(jiān)測(cè)水平。此項(xiàng)技術(shù)可以檢測(cè)河流中環(huán)境微生物隨著季節(jié)變化的情況。還可以用來(lái)對(duì)地表水和飲用水中致病菌進(jìn)行檢測(cè)以便及時(shí)預(yù)告地表水污染情況以及采取相應(yīng)有效措施避免大范圍污染 </p> <p> 儀器擺放：實(shí)時(shí)熒光定量 PCR 儀應(yīng)安放在濕度較低、灰塵較少、遠(yuǎn)離水池的平穩(wěn)臺(tái)面上，不能有強(qiáng)光直射，室內(nèi)應(yīng)通風(fēng)良好，無(wú)腐蝕性氣體 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津DNA甲基化熒光定量PCR實(shí)驗(yàn)技術(shù)服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253046357422/4616552.png"></div><p> 熒光定量PCR技術(shù)是指在PCR反應(yīng)體系中加入熒光基團(tuán)，通過(guò)熒光信號(hào)不斷累積而實(shí)現(xiàn)實(shí)時(shí)監(jiān)測(cè)PCR全程，然后通過(guò)標(biāo)準(zhǔn)曲線對(duì)未知模板進(jìn)行定量分析的方法。在實(shí)時(shí)熒光定量PCR中，對(duì)全程PCR擴(kuò)增過(guò)程進(jìn)行實(shí)時(shí)檢測(cè)，根據(jù)反應(yīng)時(shí)間和熒光信號(hào)的變化可以繪制成一條曲線 </p> <p> Ct值：C**Cycle，t**threshold，Ct值的含義是每個(gè)反應(yīng)管內(nèi)的熒光信號(hào)到達(dá)設(shè)定的域值時(shí)所經(jīng)歷的循環(huán)數(shù) </p> <p> 熒光背景信號(hào)階段：在熒光背景信號(hào)階段，擴(kuò)增的熒光信號(hào)與背景無(wú)法區(qū)分，無(wú)法判斷產(chǎn)物量的變化 </p></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/387682.html</p></div></div></div></div></div><div id="t2yaxeq" class="page-right twoColRt"><div id="kzrt8zr" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="laspwyq" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" alt="福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></div><p>福建ZF配件哪家強(qiáng) 信息推薦自業(yè)物資供應(yīng)</p></a></div><div id="ixprtkh" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/846569.html"><div><img 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