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href="http://www.cdcfah.com/zx/"><span>新聞資訊</span></a></li><li id = "index_article"><a href="http://www.cdcfah.com/xw/"><span>行業(yè)資訊</span></a></li><li id = "index_show"><a href="http://www.cdcfah.com/zh/"><span>展會信息</span></a></li></ul></div></nav><section class="wrap clearfix"><section class="container clearfix tcPro"><section class="home-main container clearfix"><div id="fuwd8ie" class="twoCol"><div id="vpbicnp" class="page-proshow clearfix"><p class="current"> 當前位置:<a href="http://www.cdcfah.com/">首頁?<a href="http://www.cdcfah.com/cp/">產(chǎn)品供應</a>?<a href="http://www.cdcfah.com/cp/second-_nongye/">農(nóng)業(yè)</a>?<a href="http://www.cdcfah.com/cp/third-nongye_nlmyxmhz/">農(nóng)林牧漁項目合作</a>?天津SV40法細胞永生化技術服務來電咨詢上海朝瑞生物科技供應</p><div id="z332abs" class="leftbox clearfix"><h1>天津SV40法細胞永生化技術服務來電咨詢上海朝瑞生物科技供應</h1><div id="8dkmov3" class="proBig"><div id="mamjvh9" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256615136718/5744080.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="ajqhtke" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256615136718/5744080.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="8l73wyx" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關鍵詞：</span>天津SV40法細胞永生化技術服務</p><p><span>***更新：</span>2020-06-26 02:52:36</p><p><span id="click" >瀏覽次數(shù)：1次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="ixzgxj2" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機: </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="ptam8ev" class="page-proshow02"><p class="title">詳細說明</p><div id="qpg7b3b" class="box"><p><p> 《2016年浙江省醫(yī)學會耳鼻咽喉頭頸外科學學術年會論文匯編》2016年收藏|手機打開手機客戶端打開本文大鼠橢圓囊感覺上皮細胞永生化細胞系的建立劉俊黃海林吳佳源【摘要】：目的通過SV40T抗原在大鼠橢圓囊感覺上皮細胞(utricularsensoryepithelialcell,USEC)表達,建立無限增殖細胞系(USEC-1),為毛***，天津SV40法細胞永生化技術服務、基因表達及信號轉導的研究提供豐富細胞來源。方法取出生1d(postnatalday1,P1)大鼠的橢圓囊感覺上皮,嗜熱菌蛋白酶(thermolysin)消化處理,胰酶+膠原酶消化,獲得具活性的純橢圓囊感覺上皮細胞。然后加入猿猴肉瘤***SV40(SimianVirus,SV40)轉染培養(yǎng)，天津SV40法細胞永生化技術服務,通過行態(tài)學及增殖能力的變化粗篩出USEC細胞,傳代培養(yǎng)。并通過顯微鏡及透射電鏡對細胞的行態(tài)、生長特征觀察;細胞角蛋白18、波形蛋白的表達;RT-PCR檢測SV40T抗原;細胞計數(shù)分析USEC-1細胞的增殖能力;軟瓊脂糖培養(yǎng)、裸鼠致瘤試驗來鑒定永生化細胞系USEC-1的特征。結果USEC-1細胞系保持了原代細胞的表形特征,呈扁平、多角形、核大而圓的上皮細胞形態(tài),細胞之間連接緊密，天津SV40法細胞永生化技術服務,形成單層時呈"鋪路石樣"外觀?？梢娪沙砂賯€SEC-1細胞包繞液體而成的dome。細胞角蛋白表達陽性,波形蛋白表達陰性。不同世代USEC-1均表達SV40T抗原。該系已培養(yǎng)6個月。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津SV40法細胞永生化技術服務" src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256615136718/5744080.png"></div><p> 實驗證明端粒酶的活性與抑*基因表達產(chǎn)物的下調(diào)有相關關系，推測抑*基因的失活協(xié)同端粒酶的***共同參與細胞永生化進程。目前有多種細胞永生化的方法，但不是每一種方法都可使一個特定的細胞永生化。由于細胞的來源動物、組織不同，其細胞的增殖、分化調(diào)控也不盡相同，因此，每類細胞的永生化的分子機制不同，其永生化細胞的方法也不同。優(yōu)勢細胞永生化服務永生化效率高，國際先進技術平臺，專業(yè)定制，售后保障北京安必奇生物的研究團隊在細胞永生化服務方面，擁有多年的研究經(jīng)驗，已經(jīng)成功建立了多種永生化細胞株，包括人源、鼠源、牛源及犬源細胞。安必奇生物引進其先進的細胞永生化定制平臺，依靠多位經(jīng)驗豐富的研究人員，能夠為國內(nèi)科研工作者提供專業(yè)的永生化細胞定制服務。您可以提供自主分離的特定原代細胞，也可以從我們的原代細胞產(chǎn)品中選擇合適的目的細胞。詢價及訂購感謝您選擇我公司的細胞永生化服務。如有任何相關的問題，請您聯(lián)系我們的工作人員，我們即刻為您解答和服務。參考文獻：莊金秋,梅建國,王文秀,沈志強.細胞永生化技術及其應用研究進展[J].生命科學研究,2011,15(4):363-368.王珊珊,王偉,于瑩瑩,李輝。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津SV40法細胞永生化技術服務" src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256583232422/1705951.png"></div><p> HSAS4，規(guī)格：>5×100000cells/瓶Cs-(004)-006833大長舌菓蝠肺細胞；DBL1，規(guī)格：>5×100000cells/瓶Cs-(004)-006834恒河猴肺細胞；RM-L1，規(guī)格：>5×100000cells/瓶Cs-(004)-006835小鼠肺成纖維細胞；WML2，規(guī)格：>5×100000cells/瓶Cs-(004)-006836小鼠胚胎細胞；BABL/C-E，規(guī)格：>5×100000cells/瓶Cs-(004)-006837大長舌菓蝠肌肉細胞；DBM1，規(guī)格：>5×100000cells/瓶Cs-(004)-006838豚鼠肺細胞；GP-F1，規(guī)格：>5×100000cells/瓶Cs-(004)-006839豚鼠皮膚細胞；GP-S1，規(guī)格：>5×100000cells/瓶Cs-(004)-006840豚鼠皮膚細胞；GP-S2，規(guī)格：>5×100000cells/瓶Cs-(004)-006841豚鼠心肌細胞；GP-H1，規(guī)格：>5×100000cells/瓶Cs-(004)-006842豚鼠皮膚細胞；GP-S3，規(guī)格：>5×100000cells/瓶Cs-(004)-006843敘利亞倉鼠肌肉細胞；GH-M1，規(guī)格：>5×100000cells/瓶Cs-(004)-006844敘利亞倉鼠皮膚細胞；GH-S1，規(guī)格：>5×100000cells/瓶Cs-(004)-006845大鼠肺細胞；WTRL1，規(guī)格：>5×100000cells/瓶Cs-(004)-006846小耳豬肺細胞；SEP-L1，規(guī)格：>5×100000cells/瓶Cs-(004)-006847小耳豬肺細胞；SEP-L2，規(guī)格：>。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/393572.html</p></div></div></div></div></div><div id="rg2nxjn" class="page-right twoColRt"><div id="lv9vbse" class="box01 margin10 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