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src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256581992187/3487203.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="xmegd7j" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>上海EBV法細胞永生化構(gòu)建服務</p><p><span>***更新：</span>2020-06-26 11:21:30</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="ot7hovc" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機: </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="7vv3gi7" class="page-proshow02"><p class="title">詳細說明</p><div id="baspbtf" class="box"><p><p> 建立人卵巢*永生化細胞系,為卵巢*體外研究提供實驗模型。方法利用基因重組技術(shù)構(gòu)建猴腎virus40(SV40)T抗原基因的gaoxiao真核表達載體pcD2SV40。將其轉(zhuǎn)染10例原代培養(yǎng)的早期傳代細胞,經(jīng)G418篩選,抗性克隆擴大培養(yǎng),建立永生化細胞系。免疫組化檢測細胞的上皮起源，上海EBV法細胞永生化構(gòu)建服務,用PCR、RTPCR和SouthernBlot方法鑒定SV40T基因在轉(zhuǎn)染細胞中的表達。觀察所建立的永生化細胞系的染色體核型。結(jié)果10例轉(zhuǎn)染細胞中,6例篩選出抗性克隆，上海EBV法細胞永生化構(gòu)建服務。角蛋白免疫組化證實3例為上皮起源。其中2例上皮起源細胞得以擴大培養(yǎng)成系,長期穩(wěn)定傳代,分別命名為BUPH:OVSC2和BUPH:OVCA3。經(jīng)鑒定兩種細胞系中存在SV40T在基因水平及轉(zhuǎn)錄水平的表達，上海EBV法細胞永生化構(gòu)建服務。其染色體核型均符合人體惡性細胞特征。結(jié)論利用SV40T抗原基因進行人卵巢*原代細胞的永生化是一種可行的、可重復的建系方法,可提高卵巢*細胞成系機率。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海EBV法細胞永生化構(gòu)建服務" src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256581992187/3487203.png"></div><p> 眾所周知，原代細胞**真實地反映特定細胞在體內(nèi)的生理狀態(tài)。然而，它們一般傳不了幾代就會停止增殖并死亡。對于一些需要大量細胞進行的實驗比如生化分析，遺傳篩選等，無法使用原代細胞。這阻礙了原代細胞在實驗室的應用。另外，一些稀有疾病獲得的血液或者組織樣本比較少，導致獲得的細胞數(shù)目比較有限。限制了后續(xù)實驗的正常進行。而對于可以無限傳代的細胞系，雖然沒有衰老的問題，但它們通常包含許多遺傳突變，表現(xiàn)出不穩(wěn)定的核型，蛋白質(zhì)表達模式也與細胞類型不相稱。問題來了，是否有一種辦法可以使原代細胞獲得永生化的同時維持正常細胞的生理特性呢？引起細胞永生化的因素很多，其中端粒酶在這一過程中發(fā)揮重要作用。端粒是真核細胞染色體末端的一種特殊結(jié)構(gòu)。由端粒DNA和端粒蛋白質(zhì)組成。正常動物細胞DNA的端粒隨著細胞分裂而縮短，當縮短到一定長度時細胞將停止增殖并死亡。將原代細胞轉(zhuǎn)染端粒酶逆轉(zhuǎn)錄酶cDNA（humantelomerasereversetranscriptase,hTERT），可以***端粒酶活性，維持端粒長度，阻止染色體端粒降解，誘導細胞永生化。有研究表明，hTERT介導的永生化細胞可保持正常細胞的生理特性。通過對多種hTERT永生化細胞系研究確認。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海EBV法細胞永生化構(gòu)建服務" src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256594443359/8073132.png"></div><p style="text-indent:25px">B細胞永生化是一個歷久彌新的研究課題,如何維持B細胞體外連續(xù)傳代增殖并表達抗體的特性,對于研制***和診斷用抗體類***和制品具有重要的意義。B細胞永生化的方法包括雜交瘤技術(shù),EB***或SV40***永生化以及遺傳修飾法。永生化B細胞能快速有效的產(chǎn)生單克隆抗體,這種抗體能克服異源抗體產(chǎn)生的免疫排斥,是制備單克隆抗體的理想來源,成為研制***性單抗的重要方法。本文主要概述了B細胞永生化方法的研究進展與未來的發(fā)展方向,以供從事這方面研究的人員參考。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/398184.html</p></div></div></div></div></div><div id="wgnfho3" class="page-right twoColRt"><div id="rv7my3e" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="mwyqxzg" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img src="http://tyunfile.71360.com/UpLoadFile/2019/11/1/14/637082168864691056_lgpj_5374157.jpg" alt="福建ZF配件哪家強信息推薦自業(yè)物資供應" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></div><p>福建ZF配件哪家強信息推薦自業(yè)物資供應</p></a></div><div id="mw8hx3w" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/846569.html"><div><img 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