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id="vfcolk8" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>天津**基因熒光定量PCR技術(shù)服務(wù)</p><p><span>***更新：</span>2020-06-26 12:22:00</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="r7fsg87" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="wvn7e53" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="r2mg2oa" class="box"><p><p> PCR檢測(cè)方法在臨床醫(yī)學(xué)及實(shí)驗(yàn)室檢驗(yàn)中**有價(jià)值的應(yīng)用領(lǐng)域就是對(duì)***性疾病的診斷，天津**基因熒光定量PCR技術(shù)服務(wù)。理論上，只要樣本有一個(gè)病原體存在，天津**基因熒光定量PCR技術(shù)服務(wù)，PCR方法就可以檢測(cè)到，天津**基因熒光定量PCR技術(shù)服務(wù)。該方法對(duì)病原體的檢測(cè)解決了免疫學(xué)檢測(cè)的“窗口期”問題，常用于疾病的早期診斷，可判斷疾病是否處于隱性或亞臨床狀態(tài)。 </p> <p> 大量的熒光定量PCR研究資料表明，病原體數(shù)量與***性疾病病情的輕重程度、傳染性及***效果均有相關(guān)性。因此，通過熒光定量PCR方法得到的檢測(cè)結(jié)果，一定程度上可以準(zhǔn)確反映疾病***的情況。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津**基因熒光定量PCR技術(shù)服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253049365234/1538935.png"></div><p> 在PCR擴(kuò)增時(shí)加入一對(duì)引物的同時(shí)加入一個(gè)特異性的熒光探針，該探針為一寡核苷酸：5’端標(biāo)記一個(gè)報(bào)告熒光基團(tuán)，3’端標(biāo)記一個(gè)淬滅熒光基團(tuán)。探針完整時(shí)，報(bào)告基團(tuán)發(fā)射的熒光信號(hào)被淬滅基團(tuán)吸收;PCR擴(kuò)增時(shí)，Taq酶的5'-3'外切酶活性將探針酶切降解，使報(bào)告熒光基團(tuán)和淬滅熒光基團(tuán)分離，從而熒光監(jiān)測(cè)系統(tǒng)可接收到熒光信號(hào)。 </p> <p> 在熒光擴(kuò)增曲線上人為設(shè)定的一個(gè)值，它可以設(shè)定在熒光信號(hào)**擴(kuò)增階段任意位置上，但一般熒光閾值的設(shè)置是PCR反應(yīng)**-15個(gè)循環(huán)熒光信號(hào)標(biāo)準(zhǔn)偏差的10倍。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="天津**基因熒光定量PCR技術(shù)服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253049667969/2728037.png"></div><p> 熒光定量PCR 技術(shù)，是通過在PCR 反應(yīng)體系中加入熒光基團(tuán)，利用熒光信號(hào)的變化實(shí)時(shí)監(jiān)測(cè)PCR 擴(kuò)增反應(yīng)中每一個(gè)循環(huán)擴(kuò)增產(chǎn)物量的變化，對(duì)起始模板進(jìn)行定量分析的方法。實(shí)驗(yàn)采用兩種定量方式，即***定量和相對(duì)定量。熒光定量PCR 可用于mRNA 表達(dá)量水平檢測(cè)、MicroRNA 表達(dá)量水平檢測(cè)和基因拷貝數(shù)檢測(cè)。實(shí)驗(yàn)的方法可分為SYBR Green I 法和TaqMan 探針法。 </p> <p> <br /> </p> <p> 客戶提供提供樣本 </p> <p> 細(xì)胞：細(xì)胞數(shù)>10^6； </p> <p> 組織：總量>100 mg； </p> <p> DNA 或RNA 樣品：總量>2 μg。 </p> <p> 可選樣本引物：可由客戶提供，沒有引物的情況下，可由生工生物設(shè)計(jì)合成 </p> <p> 提供信息 </p> <p> 靶基因信息：包括基因序列或GenBank Accession Number 等；背景資料：包括生物物種信息（Human、Mouse、Rat 等）、DNA/RNA 來源、基因豐度等。**終交付交付報(bào)告 </p> 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