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id="jsu8uvc" class="twoCol"><div id="ae3gsri" class="page-proshow clearfix"><p class="current"> 當(dāng)前位置:<a href="http://www.cdcfah.com/">首頁?<a href="http://www.cdcfah.com/cp/">產(chǎn)品供應(yīng)</a>?<a href="http://www.cdcfah.com/cp/second-_nongye/">農(nóng)業(yè)</a>?<a href="http://www.cdcfah.com/cp/third-nongye_nlmyxmhz/">農(nóng)林牧漁項(xiàng)目合作</a>?江蘇HPV法細(xì)胞永生化專業(yè)定制歡迎來電上海朝瑞生物科技供應(yīng)</p><div id="thodkev" class="leftbox clearfix"><h1>江蘇HPV法細(xì)胞永生化專業(yè)定制歡迎來電上海朝瑞生物科技供應(yīng)</h1><div id="u8vfcoq" class="proBig"><div id="s7moahe" class="bigimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256590175781/9998280.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div><div id="k4nur73" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256590175781/9998280.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="3jdpmtq" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>江蘇HPV法細(xì)胞永生化專業(yè)定制</p><p><span>***更新：</span>2020-06-27 10:21:41</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="5u889v7" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="vgxewn8" class="page-proshow02"><p class="title">詳細(xì)說明</p><div id="ef8xevx" class="box"><p><p> 已傳25代,傳代時(shí)間為5-6天,傳代比為1:2-3。USEC-1細(xì)胞在無血清培養(yǎng)基中能正常生長。軟瓊脂糖培養(yǎng)、裸鼠致瘤試驗(yàn)陰性。結(jié)論本實(shí)驗(yàn)成功建立了大鼠橢圓囊上皮細(xì)胞永生化細(xì)胞系USEC-1,并進(jìn)行了初步的鑒定。本細(xì)胞系基本上保留了原代USEC細(xì)胞的表形特征,又不具有瘤細(xì)胞特征,可用于毛細(xì)胞分裂，江蘇HPV法細(xì)胞永生化專業(yè)定制、分化、再生及內(nèi)耳分子遺傳機(jī)制研究?！咀髡邌挝弧浚赫憬≈嗅t(yī)院【分類號(hào)】：R764下載全文更多同類文獻(xiàn)PDF全文下載CAJ全文下載(如何獲取全文？歡迎：購買知網(wǎng)充值卡、在線充值、在線咨詢)CAJViewer閱讀器支持CAJ、PDF文件格式，AdobeReader*支持PDF格式【相似文獻(xiàn)】中國期刊全文數(shù)據(jù)庫**條1鄭貴亮;郭維;翟所強(qiáng);;脂質(zhì)體轉(zhuǎn)染前庭橢圓囊上皮細(xì)胞的影響因素[J];山東醫(yī)藥;2007年33期2楊仲昆;;人內(nèi)耳橢圓囊內(nèi)淋巴管瓣膜及其與橢圓囊管的關(guān)系[J];解剖學(xué)通報(bào);1965年03期3楊占泉;;美尼爾氏病橢圓囊和內(nèi)淋巴管的基底膜病理[J];國外醫(yī)學(xué)，江蘇HPV法細(xì)胞永生化專業(yè)定制.耳鼻咽喉科學(xué)分冊(cè);1986年04期4;成纖維細(xì)胞生長因子受體在***鏈霉素?fù)p傷的成年鼠橢圓囊中的表達(dá)[J];國外醫(yī)學(xué)(耳鼻咽喉科學(xué)分冊(cè));1999年06期5賈宏博,于立身，江蘇HPV法細(xì)胞永生化專業(yè)定制,王奎年,戴建國,畢紅哲;橢圓囊和球囊在線加速度前庭知覺中的相互作用[J];中華航空醫(yī)學(xué)雜志;1996年04期6。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇HPV法細(xì)胞永生化專業(yè)定制" src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256590175781/9998280.png"></div><p> 建株方法續(xù)4、棄上清液，再加6ml1640全培養(yǎng)液進(jìn)行第二次洗滌，離心1500轉(zhuǎn)15分鐘。5、棄上清液，加入2ml1640全培養(yǎng)基（全培養(yǎng)基加入前，置37度水浴10分鐘），然后加入環(huán)胞霉素A（4%）和，充分混勻后移入到25平方厘米的培養(yǎng)瓶，置37度，5%CO2培養(yǎng)箱中。6、每2-3天加入3ml新鮮配制的培養(yǎng)基（1MHEPES緩沖液；15%胎牛血清（FBS）；1%青霉素和鏈霉素；PRMI1640補(bǔ)足到***）。7天左右鏡下觀察，可見淋巴細(xì)胞明顯增大，出現(xiàn)聚集現(xiàn)象。7、如果細(xì)胞增長慢，或細(xì)胞密度低，或培養(yǎng)液pH值呈酸性，吸出半量培養(yǎng)液，進(jìn)行等量換液。8、當(dāng)總量達(dá)到14ml時(shí)轉(zhuǎn)移到75ml培養(yǎng)瓶中，每2-3周加入5-10ml新鮮培養(yǎng)基。9、約6-8周，當(dāng)總量達(dá)到45ml時(shí)，置50ml離心管中，離心1500轉(zhuǎn)，10分鐘。棄上清，加入3ml凍存培養(yǎng)基（5%二甲基亞楓(dimethylsufoxide)，95%FBS）混勻，成細(xì)胞懸浮液。凍存管分裝，1ml/管，立即置零下80度，1-2小時(shí)后移入液氮罐中。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇HPV法細(xì)胞永生化專業(yè)定制" src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256581992187/1600974.png"></div><p> 二、建株方法 </p> <p> 1、取外周血3-4ml，肝素抗凝（血液室溫可放置48小時(shí)），應(yīng)用液濃度5u/ml，可加入5-10ml外周血。 </p> <p> 2、將血液與等量1640培養(yǎng)液混勻后，沿管壁漸漸加入到含有4ml淋巴細(xì)胞分離液的離心管中（混合血：淋巴細(xì)胞分離液=6：4），3000轉(zhuǎn)離心10分鐘。 </p> <p> 3、吸取白細(xì)胞層，移入另一支離心管中，加入不含血清的1640培養(yǎng)液6ml，輕輕混勻，進(jìn)行***次洗滌。1500轉(zhuǎn)離心15分鐘。 </p> <p> 4、棄上清液，再加6ml1640全培養(yǎng)液進(jìn)行第二次洗滌，離心1500轉(zhuǎn)15分鐘。 </p> <p> 5、棄上清液，加入2ml1640全培養(yǎng)基（全培養(yǎng)基加入前，置37度水浴10分鐘），然后加入環(huán)胞霉素A（4%）和1.2mlEBV液/份，充分混勻后移入到25平方厘米的培養(yǎng)瓶，置37度，5%CO2培養(yǎng)箱中。 </p> <p> 6、每2-3天加入3ml新鮮配制的培養(yǎng)基（1.6% 1M HEPES 緩沖液；15%胎牛血清（FBS）；1%青霉素和鏈霉素；PRMI 1640補(bǔ)足到**）。7天左右鏡下觀察，可見淋巴細(xì)胞明顯增大，出現(xiàn)聚集現(xiàn)象。 </p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/409798.html</p></div></div></div></div></div><div id="icurjqs" class="page-right twoColRt"><div id="y873dph" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="3zt8ik8" 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