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class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>上海細胞永生化技術(shù)服務(wù)</p><p><span>***更新：</span>2020-06-28 11:24:24</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="uimjlsr" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機: </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號昂立大廈2113室</p><p>[當前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="mbvryz3" class="page-proshow02"><p class="title">詳細說明</p><div id="thols8l" class="box"><p><p> EBV細胞永生化實驗步驟1、淋巴細胞分離液：避光4度保存，用前在37度水浴中加溫。整個分離過程中，溫度應(yīng)控制在8-28度，溫度過高或過低均影響分離質(zhì)量。2、全培養(yǎng)基（用前配置）：RPM1640培養(yǎng)基，內(nèi)含20%胎牛血清（Gibco）{56℃滅活30分鐘}，1M的HEPES，，1%青霉素和鏈霉素。3、環(huán)胞酶素A（CyA）：5ml(250mg)/瓶，用1640培養(yǎng)基稀釋成，使用時終濃度為2ug/ml()。4、EBV液購自中國科學(xué)院遺傳所，儲存于零下80度。使用時從冰箱中取出，上海細胞永生化技術(shù)服務(wù)，37度迅速融化，上海細胞永生化技術(shù)服務(wù)，用，不要超過。二，上海細胞永生化技術(shù)服務(wù)、建株方法1、取外周血3-4ml，肝素抗凝（血液室溫可放置48小時），應(yīng)用液濃度5u/ml，可加入5-10ml外周血。2、將血液與等量1640培養(yǎng)液混勻后，沿管壁漸漸加入到含有4ml淋巴細胞分離液的離心管中（混合血：淋巴細胞分離液=6：4），3000轉(zhuǎn)離心10分鐘。3、吸取白細胞層，移入另一支離心管中，加入不含血清的1640培養(yǎng)液6ml，輕輕混勻，進行次洗滌。1500轉(zhuǎn)離心15分鐘。 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海細胞永生化技術(shù)服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256615136718/5744080.png"></div><p style="text-indent:25px">細胞永生化(cell immortalization) 指體外培養(yǎng)的細胞經(jīng)過自發(fā)的或受外界因素的影響從增殖衰老危機中逃離, 從而具有無限增殖能力的過程. 自發(fā)永生化的幾率非常小, 嚙齒類動物為10-5~10-6, 而人類細胞則更為罕見, 小于10-12. 通過基因轉(zhuǎn)染等技術(shù)將外源性永生化基因, 如***、原*基因和抑*基因突變體等, 導(dǎo)入目的細胞內(nèi), 以增加永生化的發(fā)生率, 進而建立永生化細胞株(immortalized cell strains), 以達到使體外培養(yǎng)的細胞具有無限增殖能力且細胞間無差異的目的。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="上海細胞永生化技術(shù)服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637122256592958984/8981407.png"></div><p> 結(jié)果提示著端粒酶在**中起著更為復(fù)雜的作用。論文通信作者、加州大學(xué)伯克利分校分子與細胞生物學(xué)助理教授DirkHockemeyer說：“我們的研究結(jié)果對如何考慮促進**產(chǎn)生的**早過程和將端粒酶作為***靶點產(chǎn)生影響。這也意味著端粒生物學(xué)在**產(chǎn)生的早期階段發(fā)揮的作用已被極大地忽視。我們在黑色素瘤中的發(fā)現(xiàn)很可能也適合其他的**類型，這也就意味著人們會更加仔細地研究較早的端?？s短作為一種*****機制所發(fā)揮的作用。”從痣到**Hockemeyer、他在加州大學(xué)伯克利分校的同事們與加州大學(xué)舊金山分校皮膚病理學(xué)家BorisBastian及其同事合作，發(fā)現(xiàn)細胞永生化是一個兩步驟過程，**初由***端粒酶的突變引起，但這種***水平較低。該突變位于端粒酶基因上游的一個啟動子（被稱作TERT），該啟動子調(diào)節(jié)著端粒酶的表達水平。四年前，已有人報道過，大約70％的惡性黑色素瘤在TERT啟動子中具有這種相同的突變。Hockemeyer說，這種TERT啟動子突變不能產(chǎn)生足夠的端端粒酶來使得前*細胞永生化，但會延緩正常的細胞衰老，從而允許有更多的時間來讓更多的***端粒酶的變化發(fā)生。他猜測端粒酶水平足以延長**短的端粒，但不能讓它們一直具有較長的長度和保持健康。</p></p><br /><p>文章來源地址: http://www.cdcfah.com/cp/422526.html</p></div></div></div></div></div><div id="mfqhok9" class="page-right twoColRt"><div id="i872f8d" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="yxevxos" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img 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