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id="qhdkrdk" class="smallimg"><div><p class="pic"><img src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253046357422/4616552.png" class="vcenter" onerror="javascript:this.src='/static/new_net_01/images/nopic.jpg';"></p></div></div></div><div id="vmdugxd" class="content"><p><span>需求數(shù)量：</span>0</p><p><span>價(jià)格要求：</span>面議</p><p><span>所在地：</span>上海市</p><p><span>包裝要求：</span><span style="color: #f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>江蘇水體基因組熒光定量PCR課題承包</p><p><span>***更新：</span>2020-06-29 08:23:08</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="htvc3r3" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="znjawif" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="2i7r3mv" class="box"><p><p> 熒光定量PCR技術(shù)是指在PCR反應(yīng)體系中加入熒光基團(tuán)，江蘇水體基因組熒光定量PCR課題承包，通過(guò)熒光信號(hào)不斷累積而實(shí)現(xiàn)實(shí)時(shí)監(jiān)測(cè)PCR全程，然后通過(guò)標(biāo)準(zhǔn)曲線對(duì)未知模板進(jìn)行定量分析的方法。在實(shí)時(shí)熒光定量PCR中，江蘇水體基因組熒光定量PCR課題承包，對(duì)全程PCR擴(kuò)增過(guò)程進(jìn)行實(shí)時(shí)檢測(cè)，根據(jù)反應(yīng)時(shí)間和熒光信號(hào)的變化可以繪制成一條曲線 </p> <p> Ct值：C**Cycle，江蘇水體基因組熒光定量PCR課題承包，t**threshold，Ct值的含義是每個(gè)反應(yīng)管內(nèi)的熒光信號(hào)到達(dá)設(shè)定的域值時(shí)所經(jīng)歷的循環(huán)數(shù) </p> <p> 熒光背景信號(hào)階段：在熒光背景信號(hào)階段，擴(kuò)增的熒光信號(hào)與背景無(wú)法區(qū)分，無(wú)法判斷產(chǎn)物量的變化 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇水體基因組熒光定量PCR課題承包" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253046357422/4616552.png"></div><p> 熒光定量PCR(Flurogenic Quantitative Polymerase ChainReaction，F(xiàn)Q-PCR；real-time quantitative PCR, RT-qPCR or qPCR)，是1996 年由美國(guó)Applied Biosystems 公司推出的一種新定量試驗(yàn)技術(shù)，它是通過(guò)熒光染料或熒光標(biāo)記的特異性探針，標(biāo)記**PCR產(chǎn)物進(jìn)行實(shí)時(shí)監(jiān)測(cè)反應(yīng)，利用與之相適應(yīng)的軟件對(duì)產(chǎn)物進(jìn)行分析，計(jì)算待測(cè)樣品模板的初始濃度 [1] 。 </p> <p> 熒光域值(threshold)的設(shè)定：PCR反應(yīng)的前15個(gè)循環(huán)的熒光信號(hào)作為熒光本底信號(hào)，熒光域值的缺省設(shè)置是3～15個(gè)循環(huán)的熒光信號(hào)標(biāo)準(zhǔn)偏差的10倍 </p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="江蘇水體基因組熒光定量PCR課題承包" src="http://tyunfile.71360.com/UploadFile/shchaorui/637116253043593750/2359026.png"></div><p> ***定量：用已知的標(biāo)準(zhǔn)曲線對(duì)未知樣本的量進(jìn)行推算的方法為***定量法。此種方法需要明確標(biāo)準(zhǔn)樣品的濃度情況，然后稀釋標(biāo)準(zhǔn)樣品，分為幾個(gè)不同梯度濃度然后進(jìn)行反應(yīng)測(cè)試。橫坐標(biāo)為標(biāo)準(zhǔn)品拷貝數(shù)的對(duì)數(shù)值，縱坐標(biāo)為測(cè)得的Ct值，進(jìn)而繪制出標(biāo)準(zhǔn)曲線。以標(biāo)準(zhǔn)曲線為基礎(chǔ)，在定量分析未知樣品過(guò)程中根據(jù)其ct值能夠?qū)悠鹗伎截悢?shù)推算出 </p> <p> 實(shí)時(shí)熒光定量PCR技術(shù)總結(jié)為以下幾點(diǎn)。特異性強(qiáng) 靈敏度高重復(fù)性好檢測(cè)速度快自動(dòng)化程度高 </p> <p> 實(shí)時(shí)熒光定量PCR具有靈敏度高、特異性高和精確性高的優(yōu)點(diǎn)，此項(xiàng)技術(shù)已被應(yīng)用于分子生物學(xué)、醫(yī)學(xué)、食品檢測(cè)和環(huán)境監(jiān)測(cè)等多個(gè)領(lǐng)域 </p></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/432822.html</p></div></div></div></div></div><div id="eyuriu7" class="page-right twoColRt"><div id="dxe8yfe" class="box01 margin10 clearfix"><h3><a href="http://www.cdcfah.com/cp/">猜你喜歡</a></h3><div id="im27ugs" class="recomPic likePro"><a href="http://www.cdcfah.com/cp/814588.html"><div><img 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