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#f30;"></span></p><p><span>產(chǎn)品關(guān)鍵詞：</span>溫州微滴式數(shù)字PCR服務(wù)</p><p><span>***更新：</span>2020-06-29 15:27:37</p><p><span id="click" >瀏覽次數(shù)：0次</span></p><a rel="nofollow" class="button">聯(lián)系我們</a></div></div><div id="jtlnz8p" class="rightbox"><h3>公司基本資料信息</h3><p style="border:0;"><a rel="nofollow">上海朝瑞生物科技有限公司</a></p><p><span>聯(lián)系人：</span>仲先生</p><p><span>郵箱: </span>scienceladder@163.com</p><p><span>電話: </span>18621867065</p><p><span>傳真: </span>021_</p><p><span>網(wǎng)址: </span></p><p><span>手機(jī): </span>021-54133071</p><p><span>地址: </span>上海市松江區(qū)廣富林路697號(hào)昂立大廈2113室</p><p>[當(dāng)前離線] <a rel="nofollow"> [加為商友] </a><a rel="nofollow"> [發(fā)送信件] </a></p></div></div><div id="keld7yn" class="page-proshow02"><p class="title">詳細(xì)說(shuō)明</p><div id="eykhovx" class="box"><p><p style="text-indent:25px">QuantaLife公司開(kāi)發(fā)出的微滴數(shù)字PCR技術(shù)。該產(chǎn)品還獲得了2011年度Frost & Sullivan北美新產(chǎn)品創(chuàng)新獎(jiǎng)。2011年10月，Bio-Rad公司收購(gòu)了QuantaLife和ddPCR技術(shù)，相繼推出了QX100、QX200微滴式數(shù)字PCR系統(tǒng)。與其他數(shù)字PCR技術(shù)不同，Bio-Rad對(duì)樣品進(jìn)行微滴化處理。據(jù)該公司基因表達(dá)部門(mén)的銷售經(jīng)理Richard Kurtz介紹，他們的獨(dú)特優(yōu)勢(shì)是能夠產(chǎn)生非常均一、重復(fù)的1納升液滴。這樣的好處是每個(gè)樣品形成20,000個(gè)液滴，溫州微滴式數(shù)字PCR服務(wù)，而其他系統(tǒng)只能分成760-3，溫州微滴式數(shù)字PCR服務(wù)，溫州微滴式數(shù)字PCR服務(wù),000個(gè)部分。分得越多，則意味著分析越準(zhǔn)確。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="溫州微滴式數(shù)字PCR服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909691865235/2119471.jpg"></div><p style="text-indent:25px">DNase I footprinting是一種精確測(cè)定DNA結(jié)合蛋白在DNA分子上的結(jié)合位點(diǎn)的方法。凝膠阻滯實(shí)驗(yàn)（EMSA）和DNase I足跡分析實(shí)驗(yàn)是目前兩種常用體外研究核酸與蛋白相互作用的方法，凝膠阻滯實(shí)驗(yàn)可以確定轉(zhuǎn)錄因子是否與DNA直接結(jié)合，而DNase I足跡分析實(shí)驗(yàn)則可以精確鑒定其結(jié)合的DNA序列，從而幫助我們研究基因轉(zhuǎn)錄調(diào)控的機(jī)制。本技術(shù)服務(wù)利用熒光標(biāo)記法結(jié)合測(cè)序的方法來(lái)進(jìn)行DNase I足跡分析實(shí)驗(yàn)。檢測(cè)靈敏高，獲得的保護(hù)區(qū)域清晰，圖片的質(zhì)量好。</p><div style="width:100%;text-align: center;"><img style="max-width: 640px; max-height: 320px;" alt="溫州微滴式數(shù)字PCR服務(wù)" src="http://tyunfile.71360.com/UploadFile/shchaorui/637110909630185547/4431438.jpg"></div><p> 由于絕大部分微液滴中只含單個(gè)或不含模板分子，使得低豐度DNA模板分子的擴(kuò)增不受高豐度模板分子擴(kuò)增的競(jìng)爭(zhēng)，與此同時(shí)，樣品中可能存在的抑劑也在分配到微液滴的過(guò)程中進(jìn)行了相對(duì)稀釋，作用于單個(gè)微液滴內(nèi)模板擴(kuò)增的抑劑數(shù)量大幅降低，從而提高PCR擴(kuò)增對(duì)抑劑的耐受程度，因此可應(yīng)用于諸多臨床樣品（如血液、尿液、糞便、痰液、胸腹水、腦脊液等）中低豐度痕量核酸標(biāo)記物的檢測(cè)。作為表觀遺傳學(xué)研究中重要的一個(gè)研究方向——甲基化程度分析，現(xiàn)階段有不同的方法或技術(shù)來(lái)進(jìn)行研究：如傳統(tǒng)的克隆測(cè)序法、抗體檢測(cè)法、定量PCR檢測(cè)法等受限于方法學(xué)的問(wèn)題，不能獲得甲基化程度的精確定量，而數(shù)字PCR系統(tǒng)通過(guò)對(duì)樣品的微液滴處理及目標(biāo)分子的拷貝數(shù)定量，為甲基化程度的精確定量檢測(cè)提供了一種可靠的技術(shù)。 </p></p><br /><p>文章來(lái)源地址: http://www.cdcfah.com/cp/436784.html</p></div></div></div></div></div><div id="yifxzgs" class="page-right twoColRt"><div id="gvsuwy3" class="box01 margin10 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